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Chemical Research in Chinese Universities 2023年第2期39卷 202-207页

作者： li Pengyuan FENG Lu li guangyue BAI Fuquan International Joint Research Laboratory of Nano-Micro Architecture Chemistry Institute of Theoretical Chemistry and College of ChemistryJilin UniversityChangchun130021P.R.China College of Chemical Engineering North China University of Science and TechnologyTangshan063210P.R.China International Innovation Center for Forest Chemicals and Materials Nanjing Forestry UniversityNanjing210037P.R.China

The geometries and electronic structures of a series of electron donor-acceptor radical molecules have been studied *** computational results show that the introduction of substituents with strong electron donating ability into tri-(2,4,6-trichlorophenyl)methyl(TTM)radicals enables the radical molecules to form the non-Aufbau electronic *** difficulty of forming the non-Aufbau electronic structure decreases with the enhancement of the electron donating ability of the substituent,but the expansion of the molecular conjugated system is not conducive to the *** hybridization of different fragments in molecular orbitals results in the disproportionation of orbital energy level and forms a staggered energy level *** electronic structure of radical molecules can be adjusted by substituents and molecular skeleton profoundly,which is a very effective means for molecular design.

关键词： Radical emitter Non-Aufbau electronic structure Molecular orbital characteristics Density functional theory and time-dependent density functional theory(DFT and TDDFT)

Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

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Journal of Integrative Agriculture 2024年第3期23卷 948-959页

作者： Jiaxing Wei Hong Yan Jie Ren guangyue li Bo Zhang Xuenong Xu Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory for Biology of Plant Diseases and Insect Pests Beijing 100193China Key Laboratory of Natural Enemies Insects Ministry of Agriculture and Rural AffairsBeijing 100193China

The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems *** effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting *** this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on *** and its predator Neoseiulus californicus using the highly puri?ed ***1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) *** the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days *** concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the ***,neither concentration had any adverse effects on the survival or reproduction of the predatory mite *** results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological *** study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

关键词： pest management predatory mite Xcn1 morphology developmental inhibition transcriptome

A novel secreted protein FgHrip1 from Fusarium graminearum triggers immune responses in plants

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Journal of Integrative Agriculture 2024年第11期23卷 3774-3787页

作者： Zhenchao Fu Huiqian Zhuang Vincent Ninkuu Jianpei Yan guangyue li Xiufen Yang Hongmei Zeng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Fusarium graminearum,the primary pathogenic fungus responsible for Fusarium head blight(FHB)in wheat,secretes abundant chemical compounds that interact with host *** this study,a secreted protein FgHrip1,isolated from the culture filtrate of ***,was found to induce typical cell death in *** FgHrip1 gene was then cloned and expressed in Escherichia *** bioassay analysis showed that the recombinant FgHrip1 induced early defense induction events,such as reactive oxygen species(ROS)production,callose deposition,and up-regulation of defense-related genes in ***,FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae *** 6605(Pst.6605)and tobacco mosaic virus(TMV).FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB *** the expression of FgHrip1 was induced during the infection process,the deletion of the gene impaired the virulence of *** results suggest that FgHrip1triggers immunity and induces disease resistance in tobacco and wheat,thereby providing new insight into strategy for biocontrol of FHB.

关键词： FgHrip1 secreted protein plant immunity Fusarium graminearum wheat

Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1

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Science China(life Sciences) 2010年第12期53卷 1410-1417页

作者： li guangyue YANG XiuFen ZENG HongMei MAO JianJun liU Hua ZHANG YunHua QIU DeWen Key Laboratory for Biological Control of the Ministry of Agriculture Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100081China

The protein elicitor from the mycelium of Alternaria tenuissima has been *** elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species,but without causing hypersensitive *** elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton,but its mechanism is not yet *** this study,the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis.A significant difference in the relative abundances for the expression of different proteins related to metabolism,modification,regulatory,defense,stress and antioxidation was found in Arabidopsis.

关键词： protein elicitor stable isotope labelling relative abundances PeaT1

Ionic liquid-based in situ dynamically self-assembled cationic lipid nanocomplexes(CLNs)for enhanced intranasal siRNA delivery

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Chinese Chemical Letters 2024年第7期35卷 367-371页

作者： Luyu Zhang Zirong Dong Shuai Yu guangyue li Weiwen Kong Wenjuan liu Haisheng He Yi Lu Wei Wu Jianping Qi Key Laboratory of Smart Drug Delivery of MOE School of PharmacyFudan UniversityShanghai 201203China School of Pharmacy Shandong Academy of SciencesQilu University of TechnologyJi'nan 250353China College of Chemical Engineering North China University of Science and TechnologyTangshan 063210China

This research aims to develop a non-invasive strategy for small interfering RNA(siRNA)nasal delivery based on ionic liquids(ILs)and cationic lipid(2,3-dioleoyloxy-propyl)-trimethylammonium-chloride(DOTAP).Other than the classical role of penetration enhancer,ILs also acted as superior solvents to simultaneously load siRNA and DOTAP,forming siRNA-DOTAP-ILs(siRNA-DILs)*** nasal mucosa penetration,DOTAP and ILs components self-assembled into cationic lipid nanocomplexes to load siRNA for enhanced in situ *** siRNA-DILs demonstrated resistance against RNase,significant mucosa penetration,prolonged nasal retention,and satisfying gene-silencing efficacy at lower ***,DILs were also able to deliver KCa3.1-targeted siRNA effectively for the treatment of allergic rhinitis in rat model by nasal ***,DILs have great potentials to deliver biological macromolecules across nasal mucosa by in situ dynamic self-assembly.

关键词： Ionic liquids Cationic lipids lipid nanoparticles siRNA Allergic rhinitis

金属离子与乳酸菌素对沙门氏菌的协同抑菌作用

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中国食品学报 2021年第1期21卷 65-71页

作者：李光月陈漪汶李雪玲华南农业大学食品学院广州510642

通过检测唾液乳杆菌O2A发酵上清液及其所产乳酸菌素对沙门氏菌的抑菌活性,考察金属离子对乳酸菌素抑菌活性的协同作用及其机理。结果表明,单独的乳酸菌素和乳酸菌素-金属离子复配均对沙门氏菌有较好的抑菌效果;不同抑菌剂对沙门氏菌处理... 详细信息

通过检测唾液乳杆菌O2A发酵上清液及其所产乳酸菌素对沙门氏菌的抑菌活性,考察金属离子对乳酸菌素抑菌活性的协同作用及其机理。结果表明,单独的乳酸菌素和乳酸菌素-金属离子复配均对沙门氏菌有较好的抑菌效果;不同抑菌剂对沙门氏菌处理18 h后,细菌的生长曲线发生较大变化,且Zn2+(30 mmol/L)-乳酸菌素、Zn2+(30 mmol/L)-发酵上清液的抑菌效果较佳。扫描电子显微镜和透射电子显微镜结果表明,与标准沙门氏菌菌株相比,处理组的菌株细胞完整性均受到不同程度的破坏,破坏程度最严重的Zn2+(30 mmol/L)-乳酸菌素处理组,其抑菌机理可能是破坏细胞结构的完整性,使菌体细胞壁和细胞膜受到损伤,从而抑制细菌生长甚至杀死细菌。

关键词：唾液乳杆菌乳酸菌素金属离子沙门氏菌协同抑菌抑菌机理

Identification of Phoxim and Omethoate Using α-hemolysin Nanopore and Aptamers

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Identification of Phoxim and Omethoate Using α-hemolysin Na...

不同类型凝析气藏在低渗多孔介质中的相态及采收率研究

中国植物病理学会2024年学术年会

作者： li Bin WANG Junxiao ZHANG Chengling li guangyue WANG Ying Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers College of Life ScienceLinyi University Xuzhou Institute of Agricultural Sciences Shandong Dingyi Ecological Agriculture Co.LTD

Contamination of pesticides has inflicted substantial harm on human health,so it is great significance to develop rapid,ultra-sensitive,and no-labeling simultaneous detection methods for multiple ***,we show that α-hemolysin（α-HL） nanopore sensor can detect and discriminated organophosphorus pesticides of phoxim and omethoate in a single nanopore without the need for labels of the probes or purification of the pesticides in real *** specifically recognize and bind pesticides to obtain pesticide-aptamer complexes,which produce characteristic current signals during passing through the *** and omethoate are accurately distinguished by a portable instrument within minutes,and the detective sensitivity is up to femtomole level whether detected alone or *** detection limits of phoxim and omethoate are 8.13×10 M and 4.16×10 *** recoveries of phoxim and omethoate from pear,tomato,and cucumber samples are 82.0%～107.0 %and 80.0%-100.4 % respectively,with coefficient of variable below 8.0 %.The developed nanopore sensor has a powerful tool for food safety research and human health.

关键词： α-Hemolysin nanopore Aptamer Phoxim Omethoate Sensor Detection

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石油学报 2006年第1期27卷 73-76页

作者：李广月郭平林春明南京大学地球科学系江苏南京210093 西南石油大学油气藏地质及开发工程国家重点实验室四川成都610500

用凝析油含量不同的凝析气分别在PVT筒和长岩心中进行了衰竭实验,并应用超声波测试技术测试了其饱和度以及高温、高压平衡凝析油气相渗曲线和常温、低压常规油气相渗曲线。实验表明,凝析油含量高的凝析气在多孔介质中凝析油的采收率比PV... 详细信息

用凝析油含量不同的凝析气分别在PVT筒和长岩心中进行了衰竭实验,并应用超声波测试技术测试了其饱和度以及高温、高压平衡凝析油气相渗曲线和常温、低压常规油气相渗曲线。实验表明,凝析油含量高的凝析气在多孔介质中凝析油的采收率比PVT筒中约高1倍。采用不同相渗曲线及CMG数值模拟软件对长岩心衰竭实验中凝析油的采收率进行了预测,结果表明,造成凝析油采收率差别的主要原因是界面张力导致凝析油气相渗曲线的差别。在数值模拟中,应当使用真实相渗曲线。对于含凝析油低的凝析气藏,多孔介质中定容衰竭凝析油饱和度远高于PVT筒中凝析油采收率,多孔介质中蒸发现象不明显。凝析油饱和度达到最大值后,随压力的降低不再变化,这表明低渗凝析气藏中尽管凝析油含量低,其污染仍然存在,不能忽视。

关键词：凝析气藏低渗多孔介质相态相渗曲线凝析油采收率

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微波辐照对方铅矿微裂纹发育影响的模拟研究

稀有金属 2017年第4期41卷 416-421页

作者：王浩胡南李广悦李峰喻清丁德馨南华大学铀矿冶生物技术国防重点学科实验室湖南衡阳421001 中南大学资源与安全工程学院湖南长沙410083

采用颗粒流软件建立了基质为方解石、内含方铅矿颗粒的方铅矿二维模型,研究了脉冲微波功率密度、重复频率、脉宽、辐照时间对方铅矿微裂纹发育的影响。结果表明:由微波诱导产生的微裂纹集中在方铅矿和方解石的晶粒边界,并随辐照时间的... 详细信息

采用颗粒流软件建立了基质为方解石、内含方铅矿颗粒的方铅矿二维模型,研究了脉冲微波功率密度、重复频率、脉宽、辐照时间对方铅矿微裂纹发育的影响。结果表明:由微波诱导产生的微裂纹集中在方铅矿和方解石的晶粒边界,并随辐照时间的增长呈发散状向周围扩展延伸,微裂纹类型以张性裂纹为主,剪切裂纹占极少比例;在输入能量相同的情况下,微波功率密度和辐照时间对矿石颗粒的损伤程度及微裂纹的发育有重大影响,高功率密度和短辐照时间比低功率密度和长辐照时间更有利于矿石颗粒内部微裂纹的发育;在一定范围内提高重复频率、增大脉宽有助于提高矿石颗粒内部微裂纹的发育程度及增大矿石颗粒的损伤程度,这有利于后续磨矿的进行;在其他条件一定的情况下,辐照时间并不是越长越好,选定最佳的最短辐照时间对于降低矿石的力学强度及减少能耗有重大意义。

关键词：微波辐照方铅矿热力耦合微裂纹

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厦门过海隧道超前帷幕注浆方案优化研究

隧道建设（中英文） 2018年第11期38卷 1888-1894页

作者：阳云李广跃中铁隧道股份有限公司河南郑州450001 中铁隧道局集团有限公司勘察设计研究院广东广州511458

为了解决过海隧道建设过程中安全快速穿越不良地质段的问题,依托厦门地铁3号线本岛至翔安过海隧道工程,针对其不均匀软弱围岩,对超前帷幕注浆方案进行研究。针对原"99孔-25 m"设计方案存在施工效率低下的问题,通过采取以"减少钻孔、配... 详细信息

为了解决过海隧道建设过程中安全快速穿越不良地质段的问题,依托厦门地铁3号线本岛至翔安过海隧道工程,针对其不均匀软弱围岩,对超前帷幕注浆方案进行研究。针对原"99孔-25 m"设计方案存在施工效率低下的问题,通过采取以"减少钻孔、配套优化"为主的系列优化措施,确定"56孔-25 m分段前进式"超前帷幕注浆方案:1)径向加固范围为工作面及开挖轮廓线外5 m;2)纵向加固范围为25 m; 3)浆液扩散半径为1. 5 m; 4)注浆终孔间距为2. 2 m; 5)使用普通硅酸盐水泥单液浆、超细水泥-水玻璃双液浆2种注浆材料; 6)注浆终压为0. 5～1. 5 MPa。结果表明:方案优化后的扫孔效率提升至200 m/d以上,注浆效率提高3倍以上,综合施工速度平均达到1. 72 m/d,为原计划的2倍,证明了优化方案的合理性和有效性。

关键词：过海隧道软弱围岩超前帷幕注浆方案优化