Ionic liquid-based in situ dynamically self-assembled cationic lipid nanocomplexes(CLNs)for enhanced intranasal siRNA delivery

作     者：Luyu Zhang Zirong Dong Shuai Yu Guangyue Li Weiwen Kong Wenjuan Liu Haisheng He Yi Lu Wei Wu Jianping Qi Luyu Zhang;Zirong Dong;Shuai Yu;Guangyue Li;Weiwen Kong;Wenjuan Liu;Haisheng He;Yi Lu;Wei Wu;Jianping Qi

作者机构：Key Laboratory of Smart Drug Delivery of MOESchool of PharmacyFudan UniversityShanghai 201203China School of PharmacyShandong Academy of SciencesQilu University of TechnologyJi'nan 250353China College of Chemical EngineeringNorth China University of Science and TechnologyTangshan 063210China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第7期

页      面：367-371页

核心收录：

学科分类：1002[医学-临床医学] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by National Natural Science Foundation of China(No.82073801) 

主　　题：Ionic liquids Cationic lipids Lipid nanoparticles siRNA Allergic rhinitis 

摘      要：This research aims to develop a non-invasive strategy for small interfering RNA(siRNA)nasal delivery based on ionic liquids(ILs)and cationic lipid(2,3-dioleoyloxy-propyl)-trimethylammonium-chloride(DOTAP).Other than the classical role of penetration enhancer,ILs also acted as superior solvents to simultaneously load siRNA and DOTAP,forming siRNA-DOTAP-ILs(siRNA-DILs)*** nasal mucosa penetration,DOTAP and ILs components self-assembled into cationic lipid nanocomplexes to load siRNA for enhanced in situ *** siRNA-DILs demonstrated resistance against RNase,significant mucosa penetration,prolonged nasal retention,and satisfying gene-silencing efficacy at lower ***,DILs were also able to deliver KCa3.1-targeted siRNA effectively for the treatment of allergic rhinitis in rat model by nasal ***,DILs have great potentials to deliver biological macromolecules across nasal mucosa by in situ dynamic self-assembly.