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Characterization of a Novel Mesophilic Bacterial Amylase Secreted by ZW2531-1, a Strain Newly Isolated from Soil

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Chemical Research in Chinese Universities 2009年第2期25卷 198-202页

作者： WANG Yang li Fan GAO chao-hui ZHANG Ying-Jiu Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

A novel mesophilic bacterial amylase, named oligosaccharide-producing multifunctional amylase（OPMA）, was discovered and characterized. OPMA is an extracellular enzyme secreted by ZW253 1-1, a strain newly isolated from Chinese soil. It could be purified to homogeneity from the culture supernatant of ZW2531-1 by 30%-60% saturated ammonium sulfate precipitation, followed by twice Sephadex gel filtration chromatography. OPMA is a 66 kDa protein based on SDS-PAGE and has an isoelectric point（p/） at pH=5.3 by Isoelectric focusing electrophoresis（WE）. It only catalyzes the degradation of starch, rather than other alpha-l,4- and/or 1,6-glucan polysaccbarides such as fl-cyclomaltodextrin and pullulan. OPMA degraded starch to produce several oligosccharides including maltose, maltotriose, and isomaltotriose as the major end-products, and perhaps other oligosaccharides such as isomalto- tetraose, rather than glucose. OPMA exhibited optimal catalytic activity at a reaction temperature of 50 ℃ and pH=6.0, as determined by orthogonal test. Under the optimal reaction conditions, purified OPMA bad a specific activity of 13.75 U/rag. These findings suggest that OPMA could be used for the production of some oligosaccharides beneficial to the food industry and medicine.

关键词： Amylase Bacterium Oligosaccharide Purification Starch

Mutations in FgPrp6 suppressive to the Fgprp4 mutant in Fusarium graminearum

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Journal of Integrative Agriculture 2022年第5期21卷 1375-1388页

作者： li chao-hui FAN Zhi-li HUANG Xin-yi WANG Qin-hu JIANG Cong XU Jin-rong JIN Qiao-jun NWAFU-PU Joint Research Center/State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYang ling 712100P.R.China Institute of Plant Protection/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN47907USA

The pre-mRNA processing factor Prp6 is an essential component of the U4/U6.U5 tri-small nuclear ribonucleoprotein(snRNP).In a previous study,mutations were identified in the PRP6 ortholog in four suppressors of Fgprp4 that was deleted of the only kinase FgPrp4 among the spliceosome components in the plant pathogenic fungus Fusarium *** this study,we identified additional suppressor mutations in FgPrp6 and determined the suppressive effects of selected *** total,12 mutations of FgPRP6 were identified in 20 suppressors of Fgprp4 by sequencing *** three mutation sites are in the linker region of FgPrp6,seven are in the first two HAT ***-seq analysis showed that suppressor mutations on different sites caused different splicing efficiency *** suppressive effects of E308K and R230H were *** to human and fission yeast,the FgPrp6 was phosphorylated by the FgPrp4 ***,the conserved Prp4-phosphorylation sites T261,T219&T221,and predicted phosphorylation sites T199&T200 on FgPrp6 were dispensable for the function of FgPrp6 in hyphal growth and sexual reproduction but important in plant *** are required for the infectious growth of *** in wheat ***-seq analysis of the wheat lemma infected with Fgprp6/FgPRP6^(Δ199-221)-GFP or Fgprp6/FgPRP6^(Δ250-262)-GFP showed that 28 and 35% introns had splicing defects,respectively,which may be responsible for their defects in plant infection.

关键词： RNA splicing FgPrp6 suppressor plant infection phosphorylation

The day-and-night solar lighting system

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The day-and-night solar lighting system

A systematic review and meta-analysis of the Chinese literature for the treatment of achalasia

第八届中国功能材料及其应用学术会议

作者： LOU Xiu-li ZHANG Xiu-xia WEN Jun-xia JIANG Dao-jie li chao-hui

ISBN: (纸本)9783037859698

In this paper,anovel light-absorpting shade and optical fiber and long persistent luminescent phosphor materialsprovided a safe and reliable dayand-night solar lighting *** was not only save resource,reduce pollution,at the same timeintroducedsolar light into the indoorto satisfy the lighting *** novel light-absorpting shade fabricated nano-diamond film to achieve the goal of self-cleaning,corrosion-resistant and corrosion-high *** novel solarlighting system used of total reflection characteristic of optical fiber,andlong persistent luminescent phosphor materials’ short storage time,high brightness to realize the purpose of dayand-night lighting.

关键词： light-absorpting shade optical fiber materials long persistent luminescent phosphor materials

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World Journal of Gastroenterology 2008年第38期14卷 5900-5906页

作者： Lan Wang You-Ming li Lan li chao-hui Yu Department ofGastroenterology the First Affiliated Hospital Medical CollegeZhejiang University Hangzhou 310003 Zhejiang ProvinceChina

AIM: To evaluate the effect of different approaches in the treatment of achalasia in China. METHODS: We performed a systematic review and meta-analysis of Chinese literature by searching the Chinese Biomedical Database and Chinese scientific Journals database (up to March 2008). All cohort studies (controlled or uncontrolled) in which the patients were observed for more than a year were reviewed in detail. Dichotomous outcomes were reported as relative risks (RR) with 95% confidence interval (CI) for controlled trials. The efficacy in uncontrolled trials was assessed by a pooled estimate of response rate with individual studies weighted in proportion to the sample size. RESULTS: Seven controlled trials compared the effect of botulinum toxin injection (BoTx) with pneumatic dilation (PD). PD was superior to BoTx [65.2% vs 45.3%; RR 1.47 (95% CI 1.23-1.77), P linical relapse rate (BoTx 30.2% vs PD 10%, RR 0.32 (0.16-0.65), P = 0.001). Heller myotomy (HM) had superior remission rate compared to PD [HM 94.0% vs PD 64.1%, RR 1.48 (1.15-1.99), P = 0.002]. In uncontrolled trials, the effectiveness of PD was 86.6% (23.9%) vs 94.8% (10.6%) for HM. The main complications of PD were perforation and gastroesophageal reflux disease. CONCLUSION: HM is the most effective long-term treatment for patients with achalasia in China. In the future, controlled clinical trials on the treatment of achalasia should focus on comparing laparoscopic myotomy with or without antireflux procedure,including different partial and total fundoplication techniques.

关键词： Meta-analysis Achalasia Esophageal Treatment Pneumatic dilation Botulinum toxin injection Laparoscopic myotomy

Tissue-engineered conduit using bladder acellular matrix and bladder epithelial cells for urinary diversion in rabbits

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Chinese Medical Journal 2013年第2期126卷 335-339页

作者： liAO Wen-biao SONG chao li Yong-wei YANG Si-xing MENG lin-chao li Xin-hui Department of Urology Renmin Hospital of Wuhan UniversityWuhan Hubei 430060 China

Background For muscle invasive bladder cancer, radical cystectomy is the most effective treatment now and urinary diversion is often necessary. The use of intestinal tissue for urinary diversion is frequently associated with complications. In this study, we aimed to make a tissue-engineered conduit （TEC） using bladder epithelial cells and bladder acellular matrix （BAM） for urinary diversion in rabbits. Methods Bladder epithelial cells of rabbit were cultivated and expanded in vitro, then seeded on BAM, and cultured for 7 days. Then cell-seeded graft was used to make TEC. In the experimental group, most of bladder of the rabbit was removed while bladder trigone was retained. The proximal end of TEC was anastomosed with bladder trigone and the distal end was anastomosed with the abdominal stoma. In the control group, TEC was made using unseeded BAM. Haematoxylin and eosin staining was conducted, respectively, at 1, 2, 4, and 8 weeks postoperatively. Immunohistochemistry was performed 8 weeks postoperatively. Intravenous urography, retrograde pyelography, and cystoscopy of TEC were made at 12 weeks postoperatively. Results All animals were alive in the experimental group. Haematoxylin and eosin staining showed epithelial coverage in TEC. Immunohistochemistry showed anti-cytokeratin AEI/AE3 antibody and anti-ZO1 antibody positive, confirming there were mature and functional epithelial cells on the lumen of TEC. Retrograde pyelography and intravenous urography showed that TEC developed well and that there was no obstruction. In the control group, four rabbits were dead within 2 weeks and scar formation, atresia, and severe hydronephrosis were found. Conclusions We successfully made TEC using BAM and bladder epithelial cells for urinary diversion in rabbits. The lumen of this new TEC covered mature epithelial cells and could prevent urinary extravasation.

关键词： bladder cancer bladder acellular matrix epithelial cells urinary diversion tissue engineer

The super capacitor with Nano-SiC film electrode

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The super capacitor with Nano-SiC film electrode

Decoding the nexus:branched-chain amino acids and their connection with sleep,circadian rhythms,and cardiometabolic health

第八届中国功能材料及其应用学术会议

作者： ZHANG Xiu-xia li chao-hui HUANG Xin-hua LOU Xiu-li WEN Jun-xia JIANG Dao-jie JIANG Yong GENG Gui-hong School of Electronics and Information Engineering Northnational university School of Material and Engineering Northnational university

ISBN: (纸本)9783037859698

Nano-SiC was mixed with nanocrystalline graphite and ethylic cellulose to *** order to fabricate Nano-SiC film electrode of super capacitor,a series of sol-gel Nano-SiC film with different proportions are designed test ***-SiC film was fixed on metal substrates using three-steps sintering *** perovskite structure CaCuTiO(of CCTO) ceramics has giant permittivity,low dielectric loss,good thermal stability and dielectric constant in a wide temperature range has very broad application prospects in *** this paper,the solid state reaction sintering can be divided into two categories:sintering at 1373 K at different times;first heating to 1423 K then immediately cooling to 1373 K and keeping for different *** constantanddielectric lossdecreasedwith frequencyrises.A super capacitor was designed based on CCTO ceramics dielectric and Nano-SiC film electrode.

关键词： Nano-SiC Film electrode super capacitor CCTO ceramicsdielectric

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Neural Regeneration Research 2025年第5期20卷 1350-1363页

作者： hui li Laurent Seugnet Department of Neurology Xijing HospitalXi’anShaanxi ProvinceChina Centre de Recherche en Neurosciences de Lyon Integrated Physiology of the Brain Arousal Systems(WAKING)UniversitéClaude Bernard Lyon 1INSERM U1028CNRS UMR 5292BronFrance

The sleep-wake cycle stands as an integrative process essential for sustaining optimal brain function and,either directly or indirectly,overall body health,encompassing metabolic and cardiovascular *** the heightened metabolic activity of the brain,there exists a considerable demand for nutrients in comparison to other *** these,the branched-chain amino acids,comprising leucine,isoleucine,and valine,display distinctive significance,from their contribution to protein structure to their involvement in overall metabolism,especially in cerebral *** the first amino acids that are released into circulation post-food intake,branched-chain amino acids assume a pivotal role in the regulation of protein synthesis,modulating insulin secretion and the amino acid sensing pathway of target of ***-chain amino acids are key players in influencing the brain's uptake of monoamine precursors,competing for a shared *** their involvement in protein synthesis,these amino acids contribute to the metabolic cycles ofγ-aminobutyric acid and glutamate,as well as energy ***,they impact GABAergic neurons and the excitation/inhibition *** rhythmicity of branchedchain amino acids in plasma concentrations,observed over a 24-hour cycle and conserved in rodent models,is under circadian clock *** mechanisms underlying those rhythms and the physiological consequences of their disruption are not fully *** sleep,obesity,diabetes,and cardiovascular diseases can elevate branched-chain amino acid concentrations or modify their oscillatory *** mechanisms driving these effects are currently the focal point of ongoing research efforts,since normalizing branched-chain amino acid levels has the ability to alleviate the severity of these *** this context,the Drosophila model,though underutilized,holds promise in shedding new light on these *** findings indicate its po

关键词： branched-chain amino acids cardiovascular health circadian clock drosophila insulin metabolism sleep γ-aminobutyric acid

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沉默——日本人的非语言交流

思想战线 2006年第1期32卷 111-117页

作者：李朝辉中央民族大学国际教育学院北京100081

沉默是日本人社会交际时的重要组成部分,它可以传情达意,具有丰富的内涵。对日本人来说,相对于言语,沉默所传递的信息更能体现说话者的意图。在日本,沉默现象是高度依赖语境的传统文化的遗存,同时它也反映出日本人的集团主义和耻感文化... 详细信息

沉默是日本人社会交际时的重要组成部分,它可以传情达意,具有丰富的内涵。对日本人来说,相对于言语,沉默所传递的信息更能体现说话者的意图。在日本,沉默现象是高度依赖语境的传统文化的遗存,同时它也反映出日本人的集团主义和耻感文化的主题以及“以心传心”的文化直觉。

关键词：日本传统文化沉默现象非语言现象文化直觉耻感文化

Context and Teaching of EFL Reading

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海外英语 2014年第23期 126-127页

作者：李超慧 li Tao 鲁东大学外国语学院

Both linguistics and language teaching take language as their subject. linguistics can provide language teaching with theories and language teaching can further prove the validity of linguistics. To some extent, linguistics is more theoretical while language teaching is more practical. Since linguistics is defined as the scientific study of language, it seems obvious that such a study would have direct relations to language teaching. This paper attempts to make an analysis of difference between Contextual theory of meaning and the teaching of EFL reading.

关键词： context contextual theory EFL reading discourse an