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Structure of precursor micro RNA＇s terminal loop regulates human Dicer＇s dicing activity by switching DExH/D domain

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Protein & Cell 2015年第3期6卷 185-193页

作者： Zhongmin liu Jia {1. Gang li Hong-Wei {1. department of Bio{1. and Molecular Biology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China Tsinghua-Peking Joint Center for life Sciences School of Life Sciences Tsinghua University Beijing 100084 China

Almost all pre-miRNAs in eukaryotic cytoplasm are recognized and processed into double-stranded microRNAs by the endonuclease Dicer protein comprising of multiple domains. As a key player in the small RNA induced gene silencing pathway, the major domains of Dicer are conserved among different species with the exception of the N-terminal components. Human Dicer＇s N-terminal domain has been shown to play an autoinhibitory function of the protein＇s dicing activity. Such an auto-inhibition can be released when the human Dicer protein dimerizes with its partner protein, such as TRBP, PACT through the N-terminal DExH/D （ATPase- helicase） domain. The typical feature of a pre-miRNA contains a terminal loop and a stem duplex, which {1.d to human Dicer＇s DExH/D （ATPase-helicase） domain and PAZ domain respectively during the dicing reaction. Here, we show that pre-miRNA＇s terminal loop can regulate human Dicer＇s enzymatic activity by interacting with the DExH/D （ATPase-helicase） domain. We found that various editing products of pre-miR-1.1.by the ADAR1.1.0 protein, an A-to-I editing enzyme that modifies pre-miRNAs sequence, have different terminal loop structures and different activity regulatory effects on human Dicer. Single particle electron microscopy reconstruction revealed that pre-miRNAs with different terminal loop structures induce human Dicer＇s DExH/D （ATPase-helicase） domain into different conformational states, in correlation with their activity regulatory effects.

关键词： human Dicer DExH/D (ATPase-helicase)domain pre-miRNA-1.1 A-to-I editing single particle electron microscopy

Coupling effect of Brownian motion and laminar shear flow on colloid coagulation:a Brownian dynamics simulation study

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Chinese Physics B 2012年第5期21卷 364-371页

作者：徐升华孙祉伟李旭 Jin Tong wang Key Laboratory of Microgravity Institute of Mechanics Chinese Academy of SciencesBeijing 100190China National Microgravity Laboratory Institute of MechanicsChinese Academy of SciencesBeijing 100190China department of Physics Southern University and A&M CollegeBaton RougeLA 70813USA

Simultaneous orthokinetic and perikinetic coagulations（SOPCs） are studied for small and large Peclet numbers（P e） using Brownian dynamics *** results demonstrate that the contributions of the Brownian motion and the shear flow to the overall coagulation rate are basically not *** the early stages of coagulation with small Peclet numbers,the ratio of overall coagulation rate to the rate of pure perikinetic coagulation is proportional to P 1.2 e,while with high Peclet numbers,the ratio of overall coagulation rate to the rate of pure orthokinetic coagulation is proportional to P 1.2 ***,our results show that the aggregation rate generally changes with time for the SOPC,which is different from that for pure perikinetic and pure orthokinetic *** comparing the SOPC with pure perikinetic and pure orthokinetic coagulations,we show that the redistribution of particles due to Brownian motion can play a very important role in the *** addition,the effects of redistribution in the directions perpendicular and parallel to the shear flow direction are *** perspective explains the behavior of coagulation due to the joint effects of the Brownian motion（perikinetic） and the fluid motion（orthokinetic）.

关键词： simultaneous orthokinetic and perikinetic coagulation Brownian motion shear flow,colloidal aggregation

DWI联合MRI斑块参数对老年急性脑梗死rt-PA溶栓治疗预后的预测价值

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海南医学 2024年第19期35卷 2828-2833页

作者：王瑞杰李博程星瑶李磊义马煤业集团股份有限公司总医院放射科河南义马472300 四川大学华西医院放射科四川成都610041 河南科技大学第一附属医院放射科河南洛阳471000

目的探讨磁共振扩散加权成像(DWI)联合磁共振成像(MRI)斑块参数对老年急性脑梗死(ACI)重组组织型纤溶酶原激活剂(rt-PA)溶栓治疗预后的预测价值。方法前瞻性选取2021.1.至2024年1.义马煤业集团股份有限公司总医院收治1.9例行rt-PA溶... 详细信息

目的探讨磁共振扩散加权成像(DWI)联合磁共振成像(MRI)斑块参数对老年急性脑梗死(ACI)重组组织型纤溶酶原激活剂(rt-PA)溶栓治疗预后的预测价值。方法前瞻性选取2021.1.至2024年1.义马煤业集团股份有限公司总医院收治1.9例行rt-PA溶栓治疗的老年ACI患者作为研究对象,根据治疗90 d后改良Rankin量表(mRS)评分分为预后良好组和预后不良组。比较两组患者的一般资料、DWI定量参数[表观扩散系数(ADC)]及MRI斑块参数(纤维帽厚度、脂核面积、斑块面积、脂核/斑块面积),采用Logistic回归分析老年ACI患者rt-PA溶栓预后的影响因素,采用受试者工作特征(ROC)曲线分析DWI定量参数联合MRI斑块参数对rt-PA溶栓预后不良的预测价值。结果随访90 d,1.9例患者共1.脱落,1.8例患者中预后良好1.3例,预后不良35例;预后良好组和预后不良组患者的吸烟史[21.68%vs 40.00%]、饮酒史[24.48%vs 45.71.]和治疗前美国国立卫生院卒中量表(NIHSS)评分[(1..49±1.05)分vs(1..27±1.1.)分]比较差异均有统计学意义(P1.0.22.1.^(-3)mm^(2)/s、(0.64±0.1.)mm,明显小于预后良好组的(0.96±0.25)1.^(-3)mm^(2)/s、(1.02±0.33)mm,脂核面积、斑块面积、脂核/斑块面积值分别为(4.61.1.29)mm^(2)、(9.54±1.31.mm^(2)、(48.32.1..43)%,明显大于预后良好组的(2.49±0.78)mm^(2)、(8.1.±1.02)mm^(2)、(30.55±9.76)%,差异均有统计学意义(P1.,约登指数为0.767,优于各指标单独检测效能。结论DWI、MRI斑块参数与老年ACI rt-PA溶栓治疗预后独立相关,联合应用对预后不良具有较高预测价值,可作为临床预测预后的有效方案,并可为临床防治工作提供理论依据。

关键词：急性脑梗死磁共振扩散加权成像斑块参数溶栓预后预测

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老年高血压合并衰弱住院患者的营养状况调查及相关因素分析

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中华老年心脑血管病杂志 2024年第8期26卷 886-891页

作者：郑淑娟李瑜欧阳菊艳田甜米叶赛·艾尼瓦尔王亚楠王红新疆医科大学第一附属医院综合内二科乌鲁木齐830000

目的评估老年高血压合并衰弱住院患者的营养状况,探讨营养和衰弱的相关性。方法连续纳入2022.1.月至2023年1.月新疆医科大学第一附属医院收治的老年高血压患者801.,通过Fried表型衰弱量表评估患者衰弱程度,分为衰弱组(≥3分)276例、... 详细信息

目的评估老年高血压合并衰弱住院患者的营养状况,探讨营养和衰弱的相关性。方法连续纳入2022.1.月至2023年1.月新疆医科大学第一附属医院收治的老年高血压患者801.,通过Fried表型衰弱量表评估患者衰弱程度,分为衰弱组(≥3分)276例、衰弱前期组(1.3分)362例、非衰弱组(0分)1.3例。同时采用微型营养评定量表(micro nutrition assessment,MNA)对患者进行营养风险评估,记录日常生活活动能力量表(activities of daily {1.ving,ADL)评分及年龄校正Charlson合并症指数(age-adjusted Charlson comorbidity index,ACCI)评分。用logistic回归分析老年高血压患者发生衰弱的影响因素,用Spearnman相关性分析衰弱和营养相关指标的关系,用ROC曲线分析营养指标对衰弱发生的预测能力。结果3组患者年龄、平均收缩压、其他慢性病、多重用药、平均握力、小腿周径、血红蛋白、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、总胆固醇、白蛋白、25-羟维生素D[25(OH)D]、骨钙素、维生素B_(1.)、同型半胱氨酸、MNA评分、MNA评分分级、ADL评分、ACCI评分等比较,差异有统计学意义(P1.。多因素logistic回归分析显示,多重用药(OR=2.361.95%CI:1.332~4.1.3)、MNA评分≥24分(OR=0.298,95%CI:0.1.0~0.808)、ADL评分(OR=1.094,95%CI:1.028~1.1.6)、白蛋白(OR=0.963,95%CI:0.934~0.994)、25(OH)D(OR=0.989,95%CI:0.980~0.998)是老年高血压患者发生衰弱的独立影响因素(P1.。衰弱与年龄、多重用药、其他慢性病、ACCI评分呈正相关(P1.,与MNA评分、MNA评分分级、血红蛋白、白蛋白、25(OH)D、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、同型半胱氨酸、平均握力、小腿周径呈负相关(P1.。ROC曲线显示,25(OH)D、白蛋白和MNA评分预测衰弱发生的曲线下面积分别为0.607、0.588、0.700。结论老年高血压合并衰弱患者营养状况差,识别早期衰弱有赖于营养量表风险评估。衰弱患者营养不良风险高,影响因素较多,其中白蛋白、MNA评分、25(OH)D能有效预测老年高血压住院患者衰弱的发生,其中MNA营养评估预测性更强。

关键词：高血压衰弱营养不良数据相关性住院患者

Processing Seismic Ambient Noise Data to Obtain Reliable Surface Wave Dispersion Measurements

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Earthquake Research in China 2015年第2期29卷 214-224页

作者： li lingli wang Weitao Zhu liangbao Chen Haopeng wang Qingdong Miao Peng wang Jun Earthquake Administration of Anhui Province Mengcheng National Geophysical Observatory Institute of Geophysics CEA department of Geophysics School of Geodesy and GeometricsWuhan University

Ambient noise tomography is a rapidly emerging field of seismological research. This paper presents the current status of ambient noise data processing and its development history over the past several years, with the intention to explain and justify this development through salient examples. The ambient noise data processing procedure can be divided into four principal phases： ① single station data preparation; ② cross- correlation and temporal stacking; ③ measurements of dispersion curves （ performed with frequency-time analysis for both group and phase speeds） ; ④ quality control, including SNR analysis and selection of the acceptable measurements. In addition, we provide a specific solution for a better use of the seismic station data to ambient noise study.

关键词： Seismic ambient noise Cross-correlation Rayleigh surface wave Dispersion curves

Debulking treatment with CT-guided percutaneous radiofrequency ablation and hepatic artery infusion of floxuridine improves survival of patients with unresectable pulmonary and hepatic metastases of colorectal cancer

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Chinese Journal of Cancer 2014年第6期33卷 295-305页

作者： Sheng li Ni He {1. li Pei-Hong Wu department of Medical Imaging & Interventional Radiology Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine

The survival of most patients with both unresectable hepatic and pulmonary metastases of colorectal cancer is poor. In this retrospective study, we investigated the efficacy of computed tomography(CT)-guided radiofrequency ablation(RFA) and systemic chemotherapy plus hepatic artery infusion of floxuridine(HAIFUDR). Sixty-one patients were selected from 1.1.6 patients with pulmonary and hepatic metastases from colorectal cancer. Patients were treated with RFA and systemic chemotherapy plus HAI-FUDR(ablation group, n = 39) or systemic chemotherapy plus HAI-FUDR(FUDR group, n = 22). Patients in the two groups were matched by sex, age, number of metastases, and calendar year of RFA or FUDR. Survival data were evaluated by using univariate and multivariate analyses. Clinical characteristics were comparable between the two groups. All patients in the ablation group underwent RFA and chemotherapy. Median follow-up was 56.8 months. The 1., 3-, and 5-year overall survival(OS) rates were 97%, 64%, and 37%, respectively, for the ablation group, and 82%, 32%, and 1.%, respectively, for the FUDR group. The 1., 3-, and 5-year survival rates after metastasis were 97%, 49%, and 26% for the ablation group, and 72%, 24%, and 24% for the FUDR group, respectively. The median OS times were 45 and 25 months for the ablation and FUDR groups, respectively. In the multivariate analysis, treatment allocation was a favorable independent prognostic factor for OS(P = 0.001. and survival after metastasis(P = 0.009). These data suggest that the addition of RFA to systemic chemotherapy plus HAI-FUDR improves the survival of patients with both unresectable hepatic and pulmonary metastases from colorectal cancer.

关键词：结直肠癌射频消融肝动脉患者肝脏手术切除治疗灌注

2.1.—2022年山东地区不同人群中段尿分离菌的构成及药敏分析

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中国抗生素杂志 2024年第8期49卷 954-960页

作者：寇琳娜张春艳王梦园纪冰满思金伊茂礼李仁哲王世富山东大学附属儿童医院临床微生物科山东省儿童健康与疾病临床医学研究中心济南250022 山东省儿童微生物组学研究中心儿童细菌&真菌耐药监测研究协作网济南250022 滨州医学院附属医院检验科滨州256603 滕州市中心人民医院检验科滕州277500 烟台毓璜顶医院检验科烟台264000 济宁市第一人民医院检验科济宁272111

目的了解山东地区中段尿标本分离菌的构成和耐药性及患者年龄差异,为临床早期经验性尿路感染抗菌药物选择提供参考依据。方法利用WHONET5.6分析软件,回顾分析山东地区2.1.—2022年间山东省儿童组细菌耐药监测研究协作网成员单位上报... 详细信息

目的了解山东地区中段尿标本分离菌的构成和耐药性及患者年龄差异,为临床早期经验性尿路感染抗菌药物选择提供参考依据。方法利用WHONET5.6分析软件,回顾分析山东地区2.1.—2022年间山东省儿童组细菌耐药监测研究协作网成员单位上报的中段尿标本分离菌的构成及主要分离菌的药敏结果。结果共分离1.3955株菌株,分离的细菌以革兰阴性菌为主,真菌以白念珠菌为主。对年龄分层分析发现,儿童组与成人组的菌种分布差异较大,有统计学意义(χ2=509.4, P1.。儿童组分离前两位的分离菌是大肠埃希菌、屎肠球菌,而成人组分离前两位的分离菌是大肠埃希菌和肺炎克雷伯菌;不论儿童组还是成人组,女性的细菌分离率均较男性高。碳青霉烯类耐药的大肠埃希菌和肺炎克雷伯菌的占比分别为1.7%和5.7%,对大部分受试抗菌药物儿童组菌株的耐药率高于成人组。结论山东地区不同人群中段尿分离菌菌种分布不同,且不同分离菌对各种抗菌药物耐药性存在较大差异,因此对不同人群进行分离菌的分布和耐药性监测,对提高尿路感染的精准诊治水平和指导临床合理用药具有重要指导意义。

关键词：分离菌耐药率尿路感染成人儿童

Microtubule disruption in striatum induced striatonigral degeneration

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Microtubule disruption in striatum induced striatonigral deg...

肿瘤抑制基因PTEN在胃癌中的表达及其临床意义

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： bing-Yin SU~(1.*),Ya-Jie {1.ANG~2,Shu-Rong {1.~3,Yan-{1.ng ZHANG~2,Qiang GUO~2,Can WEN~2,Han-Zhi wang~{1.Sichuan Key Laboratory of Development and Regeneration,department of Histology and Embryology and Neurobiology, Chengdu Medical College,Chengdu 61.083,China 2 department of Neurobiology,Third Mi{1.tary Medical university,Chongqing 400038,China 3 department of Pathology,Chengdu Medical College,Chengdu 61.083,China

＜正＞The observation that the degree of terminal loss in the striatum appears to be more pronounced than the magnitude of SNc DA neuron loss,suggests that striatal DA nerve terminals are the primary target of the degenerative process and that neuronal death in PD may result from a "dying back" *** support for the concept of "dying back" includes the observations that in MPTP-treated monkeys the destruction of striatal terminals precedes that of SNc cell bodies,and in MPTP-treated mice,protection of striatal terminals prevents the loss of SNc DA *** we use intracranial injection method and inject microtubule disruptor to the striatum where axon terminals of dopamine neuron are *** the help of fluorescent tract tracing,immunofluorescence and immunohistochemistry,neurochemical analysis etc,we observed the series of pathological changes that occurred in both SNc and striatum,including neurodegeneration and g{1.al *** the mean time,we also analyzed the behavior of the COL-treated mice so as to assess its *** demonstrated in vivo:（1. Axonal microtubule injury can induce the degeneration of dopamine neurons in SNc（not apoptosis）,which may be caused by dysfunction of retrograde axonal transport.（2） Striatal neurons are sensitive to microtubule injury,which died three days after treatment of COL,though apoptosis appeared seven days after treatment of COL.（3） COL can directily activate microg{1.a,suggesting that it may activate microg{1.a through a dircect way in striatum,triggering its graded activation through days,accompanied by unique activation of astrocytes.（4） Threedimensional recontruction of confocal micropic picture of microg{1.a and astrocytes revealed their intimate spatial relationship, which promote us to propose the concept of inteRg1.al synapse,which may be important for their communication and physiological function.（5） The combined degeneration of striatal neurons and dopamine neurons in SNc could serve as a good m

关键词： Parkinson’s disease dopamine neuron neurodegeneration microtubule striatum multiple system atrophy

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实用肿瘤杂志 2002年第5期17卷 301-303页

作者：李航张祥福王川吴黎敏蔡庆发福建省莆田市第二医院肿瘤科福建莆田351100 福建医科大学附属协和医院肿瘤科福建福州350001 福建省莆田市第二医院病理科福建莆田351100

目的　研究第 1.号染色体同源丢失性磷酸酶 -张力蛋白基因 (phosphatase and tensin homology delet-ed on chrom osom e ten,PTEN)在胃癌组织中的表达水平以及与生物学行为的关系和对预后评价的意义。方法　以免疫组化方法检测 1. 6例... 详细信息

目的　研究第 1.号染色体同源丢失性磷酸酶 -张力蛋白基因 (phosphatase and tensin homology delet-ed on chrom osom e ten,PTEN)在胃癌组织中的表达水平以及与生物学行为的关系和对预后评价的意义。方法　以免疫组化方法检测 1. 6例胃癌组织中 PTEN蛋白的表达水平。结果　 1. 6例胃癌中 ,PTEN表达阳性 72例(6 7.9% ) ,其中弱阳性 1.例 (1. .1. ) ,强阳性 5 7例 (5 3.8% )。在胃癌组织中 ,PTEN蛋白水平与胃壁浸润深度、组织分化类型 ,临床分期等相关 (P1..8% ,但经 L og- rank时序检验示差异无显著性 (P=0 .0 72.1.。 Cox回归模型多变量分析显示 ,PTEN不是一个独立的预后指标。结论　 PTEN与胃癌的发生发展有关。

关键词：肿瘤抑制基因 PTEN 胃癌表达预后免疫组织化学