检索结果-南通市图书馆

Magnetic non-potentiality on the quiet Sun and the filigree

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2009年第8期9卷 933-944页

作者： Meng Zhao jing-Xiu Wang Chun-lan jin Gui-ping Zhou Key Laboratory of Solar Activity National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China

From the observed vector magnetic fields by the Solar Optical Telescope/ Spectro-Polarimeter aboard the satellite Hinode, we have examined whether or not the quiet Sun magnetic fields are non-potential, and how the G-band filigrees and Ca II network bright points （NBPs） are associated with the magnetic non-potentiality. A sizable quiet region in the disk center is selected for this study. The new findings by the study are as follows. （1） The magnetic fields of the quiet region are obviously non-potential. The region-average shear angle is 40°, the average vertical current is 0.016A m^-2, and the average free magnetic energy density, 2.7× 10^2erg cm^-3. The magnitude of these non-potential quantities is comparable to that in solar active regions. （2） There are overall correlations among current helicity, free magnetic energy and longitudinal fields. The magnetic non-potentiality is mostly concentrated in the close vicinity of network elements which have stronger longitudinal fields. （3） The filigrees and NBPs are magnetically characterized by strong longitudinal fields, large electric helicity, and high free energy density. Because the selected region is away from any enhanced network, these new results can generally be applied to the quiet Sun. The findings imply that stronger network elements play a role in high magnetic non-potentiality in heating the solar atmosphere and in conducting the solar wind.

关键词： Sun magnetic fields -- Sun photosphere -- Sun network bright point

The expression and binding properties of the rice WRKY68 protein in the Xa21-mediated resistance response to Xanthomonas oryzae pv. Oryzae

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2016年第11期15卷 2451-2460页

作者： YANG Shuo ZHOU Liang MIAO Liu-yang SHI Jia-nan SUN Cai-qiang FAN Wei lan jin-ping CHEN Hao LIU Li-juan DOU Shi-juan LIU Guo-zhen LI Li-yun College of Life Sciences Hebei Agricultural University Baoding 071001 P.R. China Quantum Design China Beijing 100027 P.R.China Beijing Protein Innovation Co. Ltd. Beijing 101318 P.R.China

Plant WRKY transcription factors are involved in various physiological processes, including biotic and abiotic stress responses, as well as developmental processes. In this study, the expression patterns of the WRKY68 protein during interactions between rice 4021 containing the bacterial blight resistance gene Xa21 and Xanthomonas oryzae pv. oryzae（Xoo） were investigated. A possible modified form of the WRKY68 protein appeared in the Xa21-mediated disease resistance response, and its expression levels were similar in compatible and incompatible responses, but differed significantly from that of the mock control treatment, suggesting that WRKY68 may be involved in the bacterial blight response in rice. To further understand WRKY68＇s roles in the resistance signaling pathway, WRKY68 recombinant protein was expressed in Escherichia coli and a microscale thermophoresis analysis was performed to investigate the interactions between WRKY68 and cis-elements in crucial pathogenesis-related（PR） genes. The results showed that the WRKY68 protein binds to W-boxes in the PR1 b promoter region, with an apparent dissociation constant of 25 nmol L–1, while the binding between WRKY68 and PR10 a was W-box independent. The results suggested that a possible modified form of the WRKY68 protein was induced during the interaction between rice and Xoo, which then regulated the activity of the downstream PR genes by binding with the W-boxes in the PR1 b gene＇s promoter region. Moreover, the constitutive transcription of the WRKY68 gene in dozens of rice tissues and the expression of the WRKY68 protein in leaves during all growth stages suggests that WRKY68 plays important roles in rice during normal growth processes.

关键词： rice bacterial blight WRKY transcription factor W-box Western blot microscale thermophoresis

Western blot detection of PMI protein in transgenic rice

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2016年第4期15卷 726-734页

作者： RONG Rui-juan WU Peng-cheng lan jin-ping WEI Han-fu WEI Jian CHEN Hao SHI Jia-nan HAO Yu-jie LIU Li-juan DOU Shi-juan LI Li-yun WU Lin LIU Si-qi YIN Chang-cheng LIU Guo-zhen College of Life Sciences Hebei Agricultural University Baoding 071001 P.R. China Beijing Protein Innovation Co. Ltd. Beijing 101318 P.R.China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100101 P.R. China

Phosphomannose isomerase （PMI） encoding gene manA is a desirable selective marker in transgenic research. Under- standing of its expression patterns in transgenic plant and establishing highly sensitive detection method based on immunoassay have great impacts on the application of PMI. In this study, PMI-specific monoclonal antibodies were generated using recombinant protein as immunogen, and could be used in Western blot to detect as little as 0.5 ng His-tagged PMI protein or rice expressed PMI protein in sample accounted for 0.4% of single rice grain （about 0.08 mg）. PMI protein driven by CaMV-35S promoter was detected in dozens of tested tissues, including root, stem, leaf, panicle, and seed at all developmental stages during rice growing, and PMI protein accounted for about 0.036% of total protein in the leaves at seedling stage. The established method potentially can be used to monitor PMI protein in rice grains.

关键词： transgenic rice protein expression CaMV-35S promoter phosphomannose isomerase (PMI) Western blot

Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： lan Zhang jingge Wei Xilei Liu Dai Li Xiaoqi Pang Fanglian Chen Hailong Cao ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

在线全文

学校读者我要写书评

暂无评论

带有警戒限的中位值控制图

浙江理工大学学报（自然科学版） 2005年第2期22卷 177-183页

作者：周永华金兰平浙江理工大学理学院杭州310018 浙江中医学院计算机系杭州310053

将Page的带有警戒限的平均值控制图推广到中位值控制图上,设计带有警戒限的中位值控制图,并同其常规控制图加以比较。进而制定与中位值图具有相同伪警报率的平均值控制图,在正态总体下比较了它们的统计效率,说明中位值图改进的优缺点。

关键词：警戒限控制图中位值平均值平均链长

在线全文

学校读者我要写书评

暂无评论

码头打入桩沉桩施工中欧规范比较

水运工程 2020年第9期 85-90,157页

作者：娄学谦桑登峰兰金平胡兴昊陈明杰王幸中交四航工程研究院有限公司广东广州510230 中交四航局第三工程有限公司广东湛江524009

近年来,我国的施工企业在"一带一路"沿线国家承揽了较多码头工程,但因对国外规范了解不深入,给施工开展带来很大困扰。以中国码头施工规范和欧洲挤土桩规范EN 12699为例,比较中国和欧洲两套现行规范在码头打入桩沉桩施工中部分条款之差... 详细信息

近年来,我国的施工企业在"一带一路"沿线国家承揽了较多码头工程,但因对国外规范了解不深入,给施工开展带来很大困扰。以中国码头施工规范和欧洲挤土桩规范EN 12699为例,比较中国和欧洲两套现行规范在码头打入桩沉桩施工中部分条款之差异,主要结果有:1)欧洲规范原则性条文更多,中国规范则有比较细致的定量指标;2)中国规范很多内容在欧洲规范中属于应由设计方考虑的内容;3)欧洲规范对打桩监控、试验、记录有系统的规定,而中国规范仅突出记录。比较结果对于我国施工企业开展国外码头建设有所裨益。

关键词：打入桩施工中国码头规范欧洲规范比较

Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

在线全文

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： Juan Fang Li-Hui Li Ming-Qian He Yue Ji Dong-Yan Lu Li-Bing Zhang jin-lan Yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation

水稻线粒体基因编码蛋白质在种子萌发及叶片发育过程中的表达分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

河北农业大学学报 2016年第2期39卷 1-8页

作者：兰金苹丁国涛刘钊郭亚璐郭美岑史佳楠刘国振河北农业大学生命科学学院河北保定071000 河北北方学院生命科学研究中心河北张家口075000

线粒体是真核生物中主要的能量供应细胞器,虽然多种植物的线粒体基因组序列已知,但对线粒体基因编码蛋白质的表达研究关注甚少。本研究采取基于抗体的蛋白质组学策略,制备了17个线粒体基因编码蛋白质的特异性抗体,包括NADH脱氢酶、细胞... 详细信息

线粒体是真核生物中主要的能量供应细胞器,虽然多种植物的线粒体基因组序列已知,但对线粒体基因编码蛋白质的表达研究关注甚少。本研究采取基于抗体的蛋白质组学策略,制备了17个线粒体基因编码蛋白质的特异性抗体,包括NADH脱氢酶、细胞色素C氧化酶、细胞色素C合成酶、ATP合酶和核糖体蛋白质等5类,利用免疫印迹技术(Western blot)调查了这些蛋白质在水稻种子萌发及不同生长发育时期叶片中的表达情况。结果发现,在种子萌发阶段,ATP合酶中的亚基9和核糖体蛋白质S13亚基表达上调,细胞色素C合成酶CCMB和CCMC表达下调,其余13个表达没有发生变化。在叶片发育阶段,15个蛋白质发生表达上调,且表达模式基本相似,均呈现前期较低,随发育逐渐提高,到成株期达到峰值,其中部分蛋白质在成熟期仍维持在较高的峰值强度(如NAD4、CCMFc、ATP9、RPS1、RPS4和RPS7),提示这些蛋白质在成熟后的灌浆期仍发挥一定作用,而其余蛋白质在成熟期表达量明显下降,提示其功能主要是在营养生长阶段发挥。有意思的是,在叶片发育阶段,RPS19蛋白质表达下调,ATP1蛋白质的表达稳定,它们的特征和功能值得进一步关注。本试验结果直观且相对定量地揭示了线粒体基因编码蛋白质的表达与种子萌发和叶片生长之间的关联,为深入了解其功能提供了有价值的线索。

关键词：水稻线粒体蛋白质表达基于抗体的蛋白质组学免疫印记

补阳还五汤两种提取方法中黄芪甲苷含量的比较

在线全文

学校读者我要写书评

暂无评论

时珍国医国药 2004年第7期15卷 404-405页

作者：陈金兰金萍赵文芬梁晓霞湖北省十堰市人民医院 442000

目的 :探讨两种提取方法对补阳还五汤中黄芪甲苷含量的影响。方法 :建立补阳还五汤中黄芪甲苷的高效液相色谱含量测定方法 ,比较水提液和水提醇沉液中黄芪甲苷含量的差异。结果 :两种方法所得汤剂中黄芪甲苷含量无显著性差异。结论

关键词：补阳还五汤提取方法黄芪甲苷高效液相色谱