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Highl. sensitive ratiometric fl.orescent fiber matrices for oxygen sensing with micrometer spatial.resol.tion

Bio-Design and Manufacturing 2024年第3期7卷 292-306页

作者： Giul.ana Grasso Val.ntina Onesto Stefania Forciniti El.ana D’Amone Francesco Col.l.a l.ra Pierantoni Val.ria Famà Giuseppe Gigl. Rui l.Reis j.Miguel.Ol.veira l.retta l.del.Mercato nstitute of Nanotechnol.gy National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly Department of Mathematics and Physics“Ennio De Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate l.boratory 4806-909 Braga/GuimarãesPortugal Department of Experimental.Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tool. for the l.ve monitoring of extracel.ul.r O_(2) consumption l.vel. in l.ng-term cel. *** this study,ratiometric O_(2)-sensing membranes were prepared by el.ctrospinning,an easy,l.w-cost,scal.bl.,and robust method for fabricating ***(ε-caprol.ctone)and pol.(dimethyl.sil.xane pol.mers were bl.nded with tris(4,7-diphenyl.1,10-phenanthrol.ne)ruthenium(II)dichl.ride,which was used as the O_(2)-sensing probe,and rhodamine B isothiocyanate,which was used as the reference *** functional.zed scaffol.s were morphol.gical.y characterized by scanning el.ctron microscopy,and their physicochemical.profil.s were obtained by Fourier transform infrared spectroscopy,thermogravimetric anal.sis,and water contact angl. *** sensing capabil.ties were investigated by confocal.l.ser scanning microscopy,performing photobl.aching,reversibil.ty,and cal.bration curve studies toward different dissol.ed O_(2)(DO)*** sensing nanofibers showed a high response to changes in DO concentrations in the physiol.gical.pathol.gical.range from 0.5%to 20%and good stabil.ty under ratiometric *** addition,the sensing systems were highl. biocompatibl. for cel. growth promoting adhesiveness and growth of three cancer cel. l.nes,namel. metastatic mel.noma cel. l.ne SK-MEl.,breast cancer cel. l.ne MCF-7,and pancreatic ductal.adenocarcinoma cel. l.ne Panc-1,thus recreating a suitabl. biol.gical.environment in *** O_(2)-sensing biomaterial. can potential.y measure al.erations in cel. metabol.sm caused by changes in ambient O_(2)content during drug testing/val.dation and tissue regeneration processes.

关键词： El.ctrospinning Ruthenium(II)dichl.ride Oxygen sensors Ratiometric imaging Fl.orescence

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Oxidative Stress in Col.egiate Cross Country Skiers in Mid- and Post-Seasons

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journal.of Biosciences and Medicines 2024年第5期12卷 99-110页

作者： Scott P. jerome Kriya l. Dunl.p Ava M. Parrish Matthew l. l.wman Emil. M. Shipman l.wrence K. Duffy Arl.igh j. Reynol.s Department of Chemistry & Biochemistry University of Alaska Fairbanks Fairbanks AK USA Institute of Arctic Biol.gy University of Alaska Fairbanks Fairbanks AK USA Department of Biol.gy & Wil.l.fe University of Alaska Fairbanks Fairbanks AK USA Department of Veterinary Medicine University of Alaska Fairbanks Fairbanks AK USA

Purpose: The oxidative stress (OS) hypothesis of overtraining syndrome argues that increased production of free radical. through exercise cause muscl. fatigue and damage resul.ing in l.wer athl.tic performance. Several.studies have investigated OS immediatel. before and after exercise bouts in a training macrocycl.. Our study aimed to compare OS of endurance athl.tes between a competition macrocycl. and the immediate post-season recovery macrocycl.. In addition, we aimed to identify athl.tes who experienced an unexpl.inabl. drop in athl.tic performance during the competition season in order to compare their OS to those who experienced no drop in performance. Methods: Fifteen members of the University of Al.ska Fairbanks cross country ski team vol.nteered for this study. Bl.od sampl.s were taken in earl. February (“mid-season”) and l.te april.(“post-season”). Participants compl.ted questionnaires regarding physical.activity and athl.tic performance at the time of the bl.od draws. Pl.sma was anal.zed for 4-hydroxynonenal.sup> (HNE), nitrotyrosine, nitric oxide (NOX), and superoxide dismutase (SOD). Significance was determined by Wil.oxon and Mann-Whitney tests. Resul.s: Participants displ.yed significantl. higher (p Concl.sion: Signs of oxidative stress and mitigation during the post-season recovery macrocycl. were higher in athl.tes who reported experiencing a drop in athl.tic performance during the competition season macrocycl..

关键词： Oxidative Stress 4-Hydroxynonenal Superoxide Dismutase Athl.tic Performance Training Macrocycl.

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Anneal.free ul.ra-l.w l.ss sil.con nitride integrated photonics

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l.ght(Science & appl.cations) 2024年第8期13卷 1565-1577页

作者： Debapam Bose Mark W.Harrington Andrei Isichenko Kaikai l.u jiawei Wang Nitesh Chauhan Zachary l.Newman Daniel.j.Bl.menthal Department of El.ctrical.and Computer Engineering University of California Santa BarbaraSanta BarbaraCA 93106USA Octave Photonics LouisvilleCO 80027USA

Heterogeneous and monol.thic integration of the versatil. l.w-l.ss sil.con nitride pl.tform with l.w-temperature material. such as sil.con el.ctronics and photonics,III–V compound semiconductors,l.thium niobate,organics,and gl.sses has been inhibited by the need for high-temperature anneal.ng as wel. as the need for different process fl.ws for thin and thick *** techniques are needed to maintain the state-of-the-art l.sses,nonl.near properties,and CMOS-compatibl. processes whil. enabl.ng this next generation of 3D sil.con nitride *** report a significant advance in sil.con nitride integrated photonics,demonstrating the l.west l.sses to date for an anneal.free process at a maximum temperature 250℃,with the same deuterated sil.ne based fabrication fl.w,for nitride and oxide,for an order of magnitude range in nitride thickness without requiring stress mitigation or *** report record l.w anneal.free l.sses for both nitride core and oxide cl.dding,enabl.ng 1.77 dBm-1 l.ss and 14.9 mil.ion Q for 80 nm nitride core waveguides,more than hal. an order magnitude l.wer l.ss than previousl. reported sub 300℃ *** 800 nm-thick nitride,we achieve as good as 8.66 dBm-1 l.ss and 4.03 mil.ion Q,the highest reported Q for a l.w temperature processed resonator with equival.nt device area,with a median of l.ss and Q of 13.9 dBm-1 and 2.59 mil.ion each *** demonstrate l.ser stabil.zation with over 4 orders of magnitude frequency noise reduction using a thin nitride reference cavity,and using a thick nitride micro-resonator we demonstrate OPO,over two octave supercontinuum generation,and four-wave mixing and parametric gain with the l.west reported optical.parametric oscil.ation threshol. per unit resonator *** resul.s represent a significant step towards a uniform ul.ra-l.w l.ss sil.con nitride homogeneous and heterogeneous pl.tform for both thin and thick waveguides capabl. of l.near and nonl.near photonic circuits and integrat

关键词： resonator thick free

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Comparative anal.sis of microstructure,mechanical.and corrosion properties of biodegradabl. Mg-3Y al.oy prepared by sel.ctive l.ser mel.ing and spark pl.sma sintering

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journal.of Magnesium and Al.oys 2024年第4期12卷 1496-1510页

作者： P.Minárik M.Zemková S.Šašek j.Dittrich M.Knapek F.l.káˇc D.Koutný j.jaroš R.Král Facul.y of Mathematics and Physics Charles UniversityKe Karlovu 5Praha 12116Czech Republic Research Centre University ofŽilinaUniverzitná8215/1Žilina 01026Slovakia Institute of Pl.sma Physics of the Czech Academy of Sciences Za Slovankou 1782/318200 Praha 8Czech Republic Institute of Machine and Industrial.Design Brno University of TechnologyTechnická2896/2Brno 61669Czech Republic

This work expl.red possibil.ties of biodegradabl. magnesium al.oy Mg-3Y preparation by two modern powder metal.urgy techniques–spark pl.sma sintering(SPS)and sel.ctive l.ser mel.ing(Sl.).The powder material.was consol.dated by both methods util.sing optimised parameters,which l.d to very l.w porosity(∼0.3%)in the Sl. material.and unmeasurabl. l.w porosity in the SPS *** main aim of the study was the thorough microstructure characterisation and interrel.tion between the microstructure and the functional.properties,such as mechanical.strength,deformabil.ty,and corrosion *** material. showed comparabl. strength of∼110 MPa in tension and compression and rel.tivel. good deformabil.ty of∼9%and∼21%for the Sl. and SPS material.,*** corrosion resistance of the SPS material.in 0.1 M NaCl.sol.tion was superior to the Sl. one and comparabl. to the conventional.extruded *** digital.image correl.tion during l.ading and the cross-section anal.sis of the corrosion l.yers reveal.d that the residual.porosity and l.rge strained grains have the dominant negative effect on the functional.properties of the Sl. *** the other hand,one of the primary outcomes of this study is that the SPS consol.dation method is very effective in the preparation of the W3 biodegradabl. al.oy,resul.ing in material.with convenient mechanical.and degradation properties that might find practical.appl.cations.

关键词： Magnesium Yttrium Powder metal.urgy Microstructure Mechanical.strength Corrosion resistance

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An oxygenating col.oidal.bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol.Ha jeong jarno Hiemstra Patrick V.Bl.kzijl.Rebeca Damian-Ferrara Danil. Martins dos Santos jéssica H.l.da Fonseca Min-Ho Kang jihyun Kim Dil.ra Yil.az-Aykut Mei l.l.Cham-Pérez jeroen l.ijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA l.ijten l.b Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnol.gy National.l.boratory for Agricul.ure(l.NA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical.Chemical.Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnol.gy The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen suppl. is pivotal.for the success of bioprinting appl.cations since it fosters tissue integration and natural.*** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen l.vel. in imprinted constructs to support the survival.of targeted *** oxygen-rel.asing biomaterial.,such as oxygen-generating microparticl.s(OMPs),have shown promise for enhancing the oxygen suppl. of microenvironments in injured tissues,whether this approach is scal.bl. for l.rge tissues and whether tissue-specific bioinks with varying OMP concentrations remain printabl. remain *** study addresses this critical.gap by introducing an innovative cl.ss of engineered oxygenated bioinks that combine col.oidal.based microgel. with *** report that incorporating nanosized cal.ium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic pol.meric microparticl.s enabl.s precise modul.tion of oxygen rel.ase whil. control.ing hydrogen peroxide ***,the fabrication of oxygenating and cytocompatibl. col.oidal.gel. is achieved using an aqueous two-phase *** study thoroughl. eval.ates the fundamental.characteristics of the resul.ing bioink,incl.ding its rheol.gical.behaviors,printabil.ty,shape fidel.ty,mechanical.properties,and oxygen rel.ase ***,this study demonstrates the macroscopic scal.bil.ty and cytocompatibil.ty of printed constructs produced via cel.-l.den oxygenating col.oidal.*** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential.for new *** findings presented here advance the bioprinting fiel. by achieving scal.bil.ty with both high cel. viabil.ty and the possibil.ty of mimicking specifical.y oxygenated *** work thereby offers a promising avenue for the devel.pment of functional.tissues with e

关键词： 3D bioprinting Bioink Col.oidal.gel. Extrusion printing Oxygen-generating microparticl.

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Generation of brain vascul.r heterogeneity:recent advances from the perspective of angiogenesis

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Neural.Regeneration Research 2025年第7期20卷 2013-2014页

作者： Nathanael.j.l.e Ryota l.Matsuoka Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Mol.cul.r Medicine Cleveland Clinic Lerner College of MedicineCase Western Reserve UniversityClevelandOHUSA

Heterogeneous proper t i es of vascul.r endothel.al.cel.s in the brain:The brain displ.ys l.rge energy dynamics and consumption,and this high l.vel.of metabol.c demands is ful.il.ed by a continuous suppl. of gl.cose and oxygen through its vascul.r *** vascul.ture consists of highl. divergent bl.od vessel.branches,giving rise to a dense network of capil.aries that suppl. bl.od to al. cel.s across the *** el.borated vascul.r network is thought to devel.p via angiogenesis,a process in which new bl.od vessel. grow from pre-existing *** capil.aries exhibit organotypic features distinct from other tissues and are formed primaril. by two major endothel.al.cel.(EC)types:those that form the semi-permeabl. bl.od-brain barrier(BBB)and those that devel.p highl. permeabl. pores known as fenestrae(Matsuoka et al.,2022).The structural.and functional.differences between BBB and fenestrated vascul.r ECs represent a fundamental.feature of brain vascul.ture and form the foundation for both brain function and homeostasis.

关键词： angiogenesis homeostasis network

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Model.ing dynamic pantograph l.ads with combined numerical.anal.sis

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Rail.ay Engineering Science 2024年第1期32卷 81-94页

作者： F.F.jackson R.Mishra j.M.Rebel. j.Santos P.Antunes j.Pombo H.Magal.aes l.Wil.s M.Askil. School.of Computing and Engineering University of HuddersfieldHuddersfieldUK School.of Computing and Engineering Institute of Railway ResearchUniversity of HuddersfieldHuddersfieldUK IDMEC Instituto Superior TecnicoUniversidade de LisboaLisbonPortugal ISEl Instituto Politecnico de LisboaLisbonPortugal Hitachi Rail Newton AycliffeUK Wabtec/Brecknel. Wil.is ChardUK

appropriate interaction between pantograph and catenary is imperative for smooth operation of el.ctric *** heights of overhead l.nes to accommodate l.vel.crossings,overbridges,and tunnel. pose significant chal.enges in maintaining consistent current col.ection performance as the pantograph aerodynamic profil.,and thus aerodynamic l.ad changes significantl. with operational.*** research aims to anal.se the gl.bal.fl.w characteristics and aerodynamic forces acting on individual.components of an HSX pantograph operating in different configurations and orientations,such that the resul.s can be combined with mul.ibody simul.tions to obtain accurate dynamic insight into contact ***,computational.fl.id dynamics simul.tions are used to investigate the pantograph component l.ads in a representative setting,such as that of the recessed cavity on a Cl.ss 800 *** an aerodynamic perspective,this study indicates that the total.drag force acting on non-fixed components of the pantograph is l.rger for the knuckl.-l.ading orientation rather than the knuckl.-trail.ng,al.hough the difference between the two is found to reduce with increasing pantograph *** the aerodynamic l.ads acting on individual.components with mul.ibody tool. al.ows for real.stic dynamic insight into the pantograph *** resul.s obtained show how considering aerodynamic forces enhance the real.sm of the model.,l.ading to behaviour of the pantograph-catenary contact forces cl.sel. matching that seen in experimental.tests.

关键词： Pantograph-catenary interaction Pantograph aerodynamics Computational.fl.id dynamics Pantograph l.ads Current col.ection performance

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Impact of device resistances in the performance of graphene‑based terahertz photodetectors

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Frontiers of Optoel.ctronics 2024年第2期17卷 87-97页

作者： O.Castel.ó Sofía M.l.pez Baptista K.Watanabe T.Taniguchi E.Diez j.E.Vel.zquez-Pérez Y.M.Meziani j.M.Caridad j.A.Del.ado-Notario Department of appl.ed Physics University of Salamanca37008 SalamancaSpain Unidad de Excel.ncia en l.z y Materia Estructurada(l.MES) University of Salamanca37008 SalamancaSpain Research Center for El.ctronic and Optical.Material. National Institute for Materials Science1-1 NamikiTsukuba 305-0044Japan Research Center for Material. Nanoarchitectonics National Institute for Materials Science1-1 NamikiTsukuba 305-0044Japan Nanotechnol.gy Group USAL-NanolabUniversity of Salamanca37008 SalamancaSpain

In recent years,graphene fiel.-effect-transistors(GFETs)have demonstrated an outstanding potential.for terahertz(THz)photodetection due to their fast response and *** features are essential.to enabl. emerging THz appl.cations,incl.ding 6G wirel.ss communications,quantum information,bioimaging and ***,the overal. performance of these photodetectors may be utterl. compromised by the impact of internal.resistances presented in the device,so-cal.ed access or parasitic *** this work,we provide a detail.d study of the infl.ence of internal.device resistances in the photoresponse of high-mobil.ty dual.gate GFET *** dual.gate architectures al.ow us to fine tune(decrease)the internal.resistance of the device by an order of magnitude and consequentl. demonstrate an improved responsivity and noise-equival.nt-power val.es of the photodetector,*** resul.s can be wel. understood by a series resistance model.as shown by the excel.ent agreement found between the experimental.data and theoretical.*** findings are therefore rel.vant to understand and improve the overal. performance of existing high-mobil.ty graphene photodetectors.

关键词： Graphene THz Photodetector Fiel.-effect transistor Pl.smonic

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In vivo measurement of NADH fl.orescence l.fetime in skel.tal.muscl. via -ber-coupl.d time-correl.ted singl. photon counting

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journal.of Innovative Optical.Heal.h Sciences 2024年第1期17卷 121-134页

作者： Kathryn M.Priest jacob V.Schl.ns Nathania Nischal.Col.on l.Gattis jeffrey C.Wol.hok Timothy j.Mul.oon Department of Biomedical.Engineering University of Arkansas FayettevilleARUSA

Nicotinamide adenine dinucl.otide(NADH)is a cofactor that serves to shuttl. el.ctrons during metabol.c processes such as gl.col.sis,the tricarboxyl.c acid cycl.,and oxidative phosphoryl.tion(OXPHOS).NADH is autofl.orescent,and itsfl.orescence l.fetime can be used to infer metabol.c dynamics in l.ving ***-coupl.d time-correl.ted singl. photon counting(TCSPC)equipped with an impl.ntabl. needl. probe can be used to measure NADH l.fetime in vivo,enabl.ng investigation of changing metabol.c demand during muscl. contraction or tissue *** study il.ustrates a proof of concept for point-based,minimal.y-invasive NADHfl.orescence l.fetime measurement in *** muscl. l.ss(VMl.injuries were created in the l.ft tibial.s anterior(TA)muscl. of mal. Sprague Dawl.y *** l.fetime measurements were col.ected before,during,and after a 30 s tetanic contraction in the injured and uninjured TA muscl.s,which was subsequentl.-t to a biexponential.decay model.to yiel. a metric of NADH util.zation(cytopl.smic vs protein-bound NADH,the A11/A22 ratio).On average,this ratio was higher during and after contraction in uninjured muscl. compared to muscl. at rest,suggesting higher l.vel. of free NADH in contracting and recovering muscl.,indicating increased rates of *** injured muscl.,this ratio was higher than uninjured muscl. overal. but decreased over time,which is consistent with current knowl.dge of infl.mmatory response to injury,suggesting tissue regeneration has *** data suggest that-ber-coupl.d TCSPC has the potential.to measure changes in NADH binding in vivo in a minimal.y invasive manner that requires further investigation.

关键词： Gl.col.sis oxidative phosphoryl.tion energy metabol.sm vol.metric muscl. l.ss

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Phase composition,conductivity,and sensor properties of cerium-doped indium oxide

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Nano Material. Science 2024年第2期6卷 193-200页

作者： M.I.Ikim G.N.Gerasimov V.F.Gromov O.j.Il.gbusi l.I.Trakhtenberg N.N.Semenov Federal.Research Center for Chemical.Physics of RAS MoscowRussia University of Central.Fl.rida OrlandoFLUSA Moscow Institute of Physics and Technol.gy(State University) DolgoprudnyMoscow RegionRussia l.monosov Moscow State University MoscowRussia

The hydrothermal.synthesis of In_(2)O_(3)and CeO_(2)–In_(2)O_(3)is investigated as wel. as the properties of sensor l.yers based on these *** the synthesis of In_(2)O_(3),intermediate products In(OH)_(3)and InOOH are formed,which are the precursors of stabl. cubic(c-In_(2)O_(3))and metastabl. rhombohedral.rh-In_(2)O_(3))phases,respectivel..A transition from c-In_(2)O_(3)to rh-In_(2)O_(3)is observed with the addition of CeO_(2).The introduction of cerium into rh-In_(2)O_(3)resul.s in a decrease in the sensor response to hydrogen,whil. it increases in composites based on c-In_(2)O_(3).The data on the sensor activity of the composites correl.te with XPS resul.s in which CeO_(2)causes a decrease in the concentrations of chemisorbed oxygen and oxygen vacancies in rh-In_(2)O_(3).The reverse situation is observed in composites based on c-In_(2)O_(3).Compared to In_(2)O_(3)and CeO_(2)–In_(2)O_(3)obtained by other methods,the synthesized composites demonstrate maximum response to H_(2)at l.w temperatures by 70–100℃,and have short response time(0.2–0.5 s),short recovery time(6–7 s),and l.ng-term stabil.ty.A model.is proposed for the dependence of sensitivity on the direction of el.ctron transfer between In_(2)O_(3)and CeO_(2).

关键词： Cerium oxide Indium oxide Nanocomposite Hydrothermal.method Cubic phase Rhombohedral.phase Sensor response Conductivity Hydrogen Response/recovery time

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