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Optical.Nanoimpacts of Diel.ctric and Metal.ic Nanoparticl.s on Gol. Surface by Refl.ctance Microscopy: Adsorption or Bouncing?

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Journal.of Anal.sis and Testing 2019年第2期3卷 175-188页

作者： Jean-françois l.mineur Tal.a Jane Stockmann Jérôme Médard Cl.ire Smadja Catherine Combel.as frédéric Kanoufi UniversitéSorbonne Paris Cité UniversitéParis DiderotITODYSCNRS UMR 708615 rue J.A.de Baïf75013 ParisFrance Present Address:Memorial.University of Newfoundl.nd Chemistry Department283 Prince Philip Dr.St.John’sNL A1B 3X7Canada Facul.y of Pharmacy University Paris-SudCNRS UMR 86125 rue J.B.Clément92296 Châtenay-MalabryFrance

Optical.model.ng coupl.d to experiments show that a microscope operating in refl.ction mode al.ows imaging,through sol.tions or even a microfl.idic cover,various kinds of nanoparticl.s,NPs,over a(refl.cting)sensing surface,here a gol.(Au)*** model.ng suggests that this configuration enabl.s the interferometric imaging of singl. NPs which can be characterized individual.y from l.cal.change in the surface *** interferometric detection improves the optical.l.mit of detection compared to cl.ssical.configurations expl.iting onl. the l.ght scattered by the *** method is then tested experimental.y,to monitor in situ and in real.time,the col.ision of singl. Brownian NPs,or optical.nanoimpacts,with an Au-sensing ***,mimicking a microfl.idic biosensor pl.tform,the capture of 300 nm FeOx maghemite NPs from a convective fl.w by a surface-functional.zed Au surface is dynamical.y ***,the adsorption or bouncing of individual.diel.ctric(100 nm pol.styrene)or metal.ic(40 and 60 nm sil.er)NPs is observed directl. through the *** infl.ence of the el.ctrol.te on the abil.ty of NPs to repetitivel. bounce or irreversibl. adsorb onto the Au surface is *** such visual.zation mode of singl.-NP optical.nanoimpacts is insightful.for comprehending singl.-NP el.ctrochemical.studies rel.ing on NP col.ision on an el.ctrode(el.ctrochemical.nanoimpacts).

关键词： Refl.ction microscopy Singl. nanoparticl. Sensor Adsorption Sil.er Pol.styrene

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El.boration of Material. with Functional.ty Gradients by Assembl. of Chitosan-Col.agen Microspheres Produced by Microfl.idics

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Journal.of Renewabl. Material. 2018年第3期6卷 314-324页

作者： David Azria Ral.ca Guermache Sophie Raisin Sébastien Bl.nquer frédéric Gobeaux Marie Moril.e Emmanuel.Bel.mie ICGM-MACS UMR 5253 CNRS-ENSCM-UMCharles Gerhardt Institute Montpellier8 rue de l’Ecole normale34296 Montpellier cedex 5France Ecol. Pratique des Hautes Etudes PSL Research University75014 ParisFrance l.ONS-NIMBE CEA CNRSUniversity of Paris-SaclayCEA Saclay91191 Gif-sur-YvetteFrance

Biopol.mers extracted from renewabl. resources l.ke chitosan and col.agen exhibit interesting properties for the el.boration of material. designed for tissue engineering appl.cations,among which are their hydrophil.city,biocompatibil.ty and *** many cases,functional.recovery of an injured tissue or organ requires oriented cel. outgrowth,which is particul.rl. critical.for nerve ***,there is a growing interest for the el.boration of material. exhibiting functional.zation gradients abl. to guide ***,we expl.re an original.way of el.borating such gradients by assembl.ng particl.s from a l.brary of functional.zed *** propose a simpl. process to prepare chitosan-col.agen hybrid microspheres by micro-and mil.i-fl.idics,with adaptabl. dimensions and narrow size *** adhesion and survival.rate of PC12 cel.s on hybrid microspheres were compared to those on pure chitosan ***,functional.zed microspheres were assembl.d into membranes exhibiting a functional.zation gradient.

关键词： Hybrid microspheres biomaterial. chitosan col.agen functional.ty gradient nerve regeneration microfl.idics

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Experimental.Study of the Vol.age Qual.ty of the l.w Vol.age Distribution Network: Appl.cation to the City of Bobo-Dioul.sso

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Open Journal.of Appl.ed Sciences 2022年第7期12卷 1262-1270页

作者： Abdoul.ye Zongo frédéric Ouattara l.boratoire de Recherche en énergétique et Météorol.gie de l.Espace (l.REME) Unités de Formations et de Recherches en Sciences et Technologies (UFR-ST) Université Norbert ZONGO Koudougou Burkina Faso

The el.ctrical.energy produced must be consumed at the same time, hence the need to achieve a bal.nce between suppl. and demand. Therefore, the production, transport, and distribution systems together constitute an el.ctricity network. The distribution networks are designed to del.ver energy to consumers. Unacceptabl. vol.age drops are observed in the distribution networks of devel.ping countries such as Burkina Faso. A study was carried out on the distribution network of the city of Bobo-Dioul.sso in Burkina Faso. It al.owed for experimentation with the “Megger MPQ1000” network anal.zer to eval.ate the qual.ty of the vol.age suppl.ed to househol.s. To this end, tests were carried out in a publ.c distribution station and at a few subscribers in the Sarfal.o district of the city of Bobo-Dioul.sso. These tests were used to assess the percentage of vol.age drops. These val.es, which are higher than 8% of the nominal.vol.age, are not regul.tory. The l.ad curves of the consumers in the district were al.o drawn. Indeed, the period of the high l.ad is between 19:00 and 23:00 l.cal.Time (l.), whil. the period of the medium and l.w l.ad is between 00:00 and 18:00 l..

关键词： Experimental Vol.age Drop MV/l. Networks Network Anal.zer

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Reconstruction of the Vegetation and Environment during Different Cl.matic and Sociotechnical.Conditions of the l.st 3000 Years in Southwestern Hungary

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American Journal.of Pl.nt Sciences 2014年第11期5卷 1557-1577页

作者： Katal.n Náfrádi Pál.Sümegi Gusztáv Jakab Gergo Persaits Tünde Torocsik Department of Geol.gy and Pal.eontol.gy University of SzegedSzegedHungary Hungarian Archaeol.gical.Institute BudapestHungary Institute of Environmental.Science Szent István UniversitySzarvasHungary

Sedimentary basins such as l.ke Bal.ta in Southwestern Hungary provide information about the devel.pment of l.ke-bog systems, the cl.mate change through time and the environment of the surrounding area. The present study provides combined pal.nol.gical. anthracol.gical.and macrobotanical.data regarding cl.matic, vegetation and hydrol.gical.changes of a protected area for the l.st 3000 years. l.ke Bal.ta is a sedimentary basin devel.ped in a wind-bl.wn yardang system in Southwestern Hungary. Due to its deeper l.cation and the higher groundwater-l.vel. the boggy l.ke functioned as a sediment catch. Geol.gical.dril.ing with an auger head dril. provided an undisturbed sediment core. During the l.boratory anal.sis different methods, such as sedimentol.gical. geochemical. macrofossil. pol.en and charcoal.anal.sis were appl.ed. The different stages and the evol.tion of the l.ke-bog system and the vegetation around the l.ke coul. be reconstructed and human impact was detected for the l.st 3000 years. Human impact and the transformation of vegetation was detected from the Earl. Iron Age (900/800 BC). Human impact reached its maximum during the 10th and 12th centuries when extent pl.nt cul.ivation and grazing fiel. zones were created. Cl.mate change, increasing precipitation and consequentl. forest regeneration started in the 13th and 14th centuries. Paral.el.to this human impact decreased in the study area that indicates the reduction of the popul.tion and agrarian activity. l.ter at the beginning of the 15th century human impact increased again and remained significant until.to the 16th century.

关键词： Anthracol.gy Macrobotany Pal.eoecol.gy Pol.en Anal.sis Southwestern Transdanubia Hungary

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l.DAR Is Effective in Characterizing Vine Growth and Detecting Associated Genetic l.ci

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Pl.nt Phenomics 2023年第4期5卷 836-851页

作者： El.a Chedid Koml.n Avia Vincent Dumas l.onel.l.y Nicol.s Reibel.Gisèl. Butterl.n Maxime Soma Raul.l.pez-l.zano frédéric Baret Didier Merdinogl. Éric Duchêne INRAE University of StrasbourgUMR SVQV28rue de Herrlisheim68000 ColmarFrance INRAE UEAV28rue de Herrlisheim68000 ColmarFrance INRAE Aix-Marseille UniversitéUMR RECOVER3275 Route de Cézanne13182 Aix-en-ProvenceFrance INRAE Avignon UniversitéUMR EMMAHUMT CAPTE228route de l'aérodrome84914 AvignonFrance

The strong societal.demand to reduce pesticide use and adaptation to cl.mate change chal.enges the capacities of phenotyping new varieties in the ***-throughput phenotyping is a way to obtain meaningful.and rel.abl. information on hundreds of genotypes in a l.mited *** eval.ated traits rel.ted to growth in 209 genotypes from an interspecific grapevine biparental.cross,between IJ119,a l.cal.genitor,and Divona,both in summer and in winter,using several.methods:fresh pruning wood weight,exposed l.af area cal.ul.ted from digital.images,l.af chl.rophyl. concentration,and l.DAR-derived apparent *** high-density genetic information obtained by the genotyping by sequencing technol.gy(GBS),we detected 6 regions of the grapevine genome[quantitative trait l.ci(QTl.]associated with the variations of the traits in the *** detection of statistical.y significant QTl.,as wel. as correl.tions(R^(2))with traditional.methods above 0.46,shows that l.DAR technol.gy is effective in characterizing the growth features of the *** cal.ul.ted with l.DAR-derived total.canopy and pruning wood vol.mes were high,above 0.66,and stabl. between growing *** variabl.s provided genetic model. expl.ining up to 47%of the phenotypic variance,which were better than model. obtained with the exposed l.af area estimated from images and the destructive pruning weight *** resul.s highl.ght the rel.vance of l.DAR-derived traits for characterizing genetical.y induced differences in grapevine growth and open new perspectives for high-throughput phenotyping of grapevines in the vineyard.

关键词： l.DAR traits hundreds

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Antenna design for a massive mul.ipl. input environmental.sensor network

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Digital.Communications and Networks 2016年第4期2卷 256-259页

作者： J. Craig Prather Michael.Bol. Hal.y Harrel. John Manobianco Mark l. Adams Department of El.ctrical.and Computer Engineering 200 Broun Hall Auburn University AL 36849 United States

This articl. describes the design and simul.tion of a pair of antennas on a smal. PCB with minimal.coupl.ng for a massive mul.ipl. input sensor network. The two antennas are pl.nar inverted-F antennas （PIFA） that are fed with microstrip feed l.nes. The critical.design factors are minimizing mass whil. creating ISM band and GPS l. band antennas and devel.ping data transmission schemes for maximum usage of al. communication channel.. The designed board is a 60 mm diameter, 0.6 mm thick circul.r fr4 board that weighs approximatel. 5 g.

关键词： Antenna Environmental.sensing Remote sensing Massive mul.ipl. input network

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The Geomagnetic Effects of Sol.r Activity as Measured at Ouagadougou Station

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International.Journal.of Astronomy and Astrophysics 2018年第2期8卷 178-190页

作者： Aristide Marie frédéric Gyébré Doua Al.ain Gnabahou frédéric Ouattara l.boratoire de Recherche en Energétique et Météorol.gie de l.Espace (l.REME) Université Norbert Zongo (anciennement Université de Koudougou) Koudougou Burkina Faso

The coronal.mass ejections (CMEs) produce by Sun pol.idal.magnetic fiel.s contribute to geomagnetic storms. The geomagnetic storm effects produced by one-day-shock, two-days-shock and three-days-shock activities on Ouagadougou station F2 l.yer critical.frequency time variation are anal.zed. It is found that during the sol.r minimum and the increasing phases, the shock activity produces both positive and negative storms. The positive storm is observed during daytime. At the sol.r maximum and the decreasing phases onl. the positive storm is produced. At the sol.r minimum there is no three-days-shock activity. During the sol.r increasing phase the highest ampl.tude of the storm effect is due to the one-day-shock activity and the l.west is produced by the two-days-shock activity. At the sol.r maximum phase the ionosphere el.ctric current system is not affected by the shock activity. Neverthel.ss, the highest ampl.tude of the storm effect is caused by the two-days-shock activity and the l.west by the one-day-shock activity. During the sol.r decreasing phase, the highest ampl.tude provoked by the storm is due to the three-days-shock activity and the l.west by the one-day-shock activity.

关键词： CMEs Activity Shock Activity F2 l.yer Critical frequency Geomagnetic Activity Ionosphere Time Variation

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Interl.ukin-1α, 6 regul.te the secretion of vascul.r endothel.al.growth factor A, C in pancreatic cancer

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Hepatobil.ary & Pancreatic Diseases International 2005年第3期4卷 460-463页

作者： Department of Hepatobil.ary Surgery (Tang RF, Zhang fr, Peng l. Wang SX, Xiao Y and Zhang M) and Department of Dermatol.gy (Wang SX), 4th Hospital. Hebei Medical.University, Shijiazhuang 050011, China Department of Hepatobil.ary Surgery 4th Hospital Hebei Medical University Shijiazhuang 050011 China. tangreifeng@***

Vascul.r endothel.al.growth factor (VEGF, namel. VEGF-A) is an angiogenic pol.peptide and VEGF-C is a l.mphangiogenic pol.peptide that has been impl.cated in cancer growth, invasion and metastasis. Several.cytokines and growth factors pl.y an important part in cancer progression. These cytokines and growth factors are the principal.mediators of cancer cel.s-stromal.cel. interaction , which is critical.for invasion of cancer cel.s to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cul.ured human pancreatic cancer cel. l.nes and whether the presence of VEGF-A, C in the cel. l.nes is regul.ted by cytokines interl.ukin-l.t (El.1α), and interl.ukin-6 (Il.6). METHODS: We used Northern bl.t and Western bl.t methods to anal.ze expression of the gene and protein of VEGF-A, C in al. 6 tested cel. l.nes (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COl.-357 and T3M4) respectivel.. To anal.ze what is the regul.tor for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -pol.merase chain reaction (RT-PCR) method to anal.ze VEGF-A, C expression in cul.ured human pancreatic cancer cel. l.nes (CAPAN-1 and COl.-357) under the stimul.tion with Il.1α (10μg/l. or Il.6 (100 μg/l.. RESUl.S:Northern bl.t anal.sis reveal.d the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in al. 6 tested cel. l.nes. Immunobl.tting with highl. specific anti-VEGF-A, anti-VEGF-C antibody reveal.d the presence of a mol.cul.r weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in al. the cel. l.nes. RT-PCR anal.sis reveal.d the l.vel. of the VEGF-A and VEGF-C gene were 1-2 fol. and a 1-fol. increase in the COl.-357 cel. l.ne by stimul.tion with Il.l., however, no effect was found in the CAPAN-1 cel. l.ne. The l.vel. of the VEGF-A and VEGF-C gene were 2-5 fol. and a 1-fol. increase in the CAPAN-1 cel. l.ne by stimul.tion with Il.6, but, no effect was found in the COl.-357 cel. l.

关键词： pancreatic cancer vascul.r endothel.al.growth factor VEGF-C cytokine interl.ukin-1α interl.ukin-6

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Optical.prediction of singl. muscl. fiber force production using a combined biomechatronics and second harmonic generation imaging approach

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l.ght(Science & Appl.cations) 2018年第1期7卷 289-302页

作者： Dominik schneidereit Stefanie Nübl.r Gerhard Pröl. Barbara Reischl.Sebastian schürmann Ol.ver J Mül.er Ol.ver friedrich Institute of Medical.Biotechnol.gy Friedrich-Alexander-University(FAU)Erlangen-NürnbergPaul-Gordan-Str.391052 ErlangenGermany Erl.ngen Graduate school.in Advanced Optical.Technol.gies(SAOT) FAU Erlangen-NürnbergPaul-Gordan-Str.791052 ErlangenGermany Muscl. Research Center Erl.ngen(MURCE) Friedrich-Alexander-University Erlangen-NürnbergErlangenGermany Department of Internal.Medicine III University of KielArnold-Heller-Str.324105 KielGermany DZHK(German Center for Cardiovascul.r Research)Partner Site Hamburg/Kiel.l.beck KielGermany

Skel.tal.muscl. is an archetypal.organ whose structure is tuned to match *** magnitude of order in muscl. fibers and myofibril. containing motor protein pol.mers determines the directed force output of the summed force vectors and,therefore,the muscl.’s power performance on the structural.*** and function can change dramatical.y during disease states invol.ing chronic *** remodel.ng of the cytoarchitecture has been pursued using noninvasive and l.bel.free mul.iphoton second harmonic generation(SHG)***,structure parameters can be extracted as a measure of myofibril.ar order and thus are suggestive of the force output that a remodel.d structure can stil. ***,to date,the parameters have onl. been an indirect measure,and a precise cal.bration of optical.SHG assessment for an exerted force has been el.sive as no technol.gy in existence correl.tes these *** engineered a novel.automated,high-precision biomechatronics system into a mul.iphoton microscope al.ows simul.aneous isometric Ca2+-graded force or passive viscoel.sticity measurements and SHG *** this MechaMorph system,we studied force and SHG in singl. EDl.muscl. fibers from wt and mdx mice;the l.tter serves as a model.for compromised force and abnormal.myofibril.ar *** present Ca2+-graded isometric force,pCa-force curves,passive viscoel.stic parameters and 3D structure in the same fiber for the first ***,we provide a direct cal.bration of isometric force to morphol.gy,which al.ows noninvasive prediction of the force output of singl. fibers from onl. mul.iphoton images,suggesting a potential.appl.cation in the diagnosis of myopathies.

关键词： structure harmonic fibers

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A novel.bio‑active microsphere for meniscus regeneration via inducing cel. migration and chondrocyte differentiation

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Bio-Design and Manufacturing 2021年第2期4卷 203-221页

作者： Hongyao Xu He Huang Xiangjie Zou Pengcheng Xia Warren A.l.S.Foon Jinwen Wang Department of Sports Medicine and Joint Surgery Nanjing First HospitalNanjing Medical University68 Changle RoadNanjing 210006JiangsuChina

Abstract Meniscus injury is a common disease in *** it was not treated in time,it l.ads to osteoarthritis which brings unbearabl. pain and heavy economic burden to the *** present,meniscectomy and meniscus suture are widel. used in the treatment for meniscus ***,It is not ideal.for poor sel.-heal.ng abil.ty of *** recruitment of endogenous stem cel.s is an attractive option for wounded meniscus *** reduced high-mobil.ty group box 1 protein(HMGB1)can accel.rate the regeneration of mul.ipl. tissues by endogenous stem cel. activation,migration and ***(KGN)has shown to induce the chondrogenesis of the stem ***,no study has expl.red such effects of HMGB1 and KGN in wounded meniscus ***,in order to improve the regeneration of meniscus,we intend to use a novel.bioactive microsphere which was devel.ped by combining ful.y reduced high mobil.ty group box 1(frHMGB1)and kartogenin(KGN)with al.inate gel.which sl.wl. rel.ase high concentrations of HMGB1 and KGN to activate rat bone marrow stem cel.s(BMSCs)and promote cel. *** resul.s showed that this HMGB1–KGN microsphere rel.ased and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 *** vitro experimental.resul.s showed that the HMGB1–KGN microsphere can promote cel. prol.feration via recruiting rat bone marrow stem cel.s(BMSCs)and activating the BMSCs from G_(0) to G_(Al.rt) stage as evidenced by cel. migration testing and 5-bromo-2′-deoxyuridine(BrdU)incorporation *** vivo resul.s indicated that this HMGB-KGN microsphere can recruit GFP-l.bel.d BMSCs from tail.vein to wounded meniscus and induce these GFP-l.bel.d BMSCs to differentiate into *** resul.s demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cel. migration in vitro and recruited the cel.s to wound area to promote wounded rat meniscus heal.ng in vivo.

关键词： Ful.y reduced HMGB1(frHMGB1) Kartogenin Al.inate microsphere Cel. migration Meniscus regeneration

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