A novel bio‑active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation

作     者：Hongyao Xu He Huang Xiangjie Zou Pengcheng Xia Warren A.L.S.Foon Jinwen Wang Hongyao Xu;He Huang;Xiangjie Zou;Pengcheng Xia;Warren A. L. S. Foon;Jinwen Wang

作者机构：Department of Sports Medicine and Joint SurgeryNanjing First HospitalNanjing Medical University68 Changle RoadNanjing 210006JiangsuChina 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2021年第4卷第2期

页      面：203-221页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：This work was supported by Nanjing Municipal Science and Technology Bureau International Joint Research and Development(No.201911041) Science and Technology Development Foundation of Nanjing Medical University(No.NMUB2018327) Social Development project of Jiangsu Province(No.BE2020623) 

主　　题：Fully reduced HMGB1(frHMGB1) Kartogenin Alginate microsphere Cell migration Meniscus regeneration 

摘      要：Abstract Meniscus injury is a common disease in *** it was not treated in time,it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the *** present,meniscectomy and meniscus suture are widely used in the treatment for meniscus ***,It is not ideal for poor self-healing ability of *** recruitment of endogenous stem cells is an attractive option for wounded meniscus *** reduced high-mobility group box 1 protein(HMGB1)can accelerate the regeneration of multiple tissues by endogenous stem cell activation,migration and ***(KGN)has shown to induce the chondrogenesis of the stem ***,no study has explored such effects of HMGB1 and KGN in wounded meniscus ***,in order to improve the regeneration of meniscus,we intend to use a novel bioactive microsphere which was developed by combining fully reduced high mobility group box 1(frHMGB1)and kartogenin(KGN)with alginate gel which slowly release high concentrations of HMGB1 and KGN to activate rat bone marrow stem cells(BMSCs)and promote cell *** results showed that this HMGB1–KGN microsphere released and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 *** vitro experimental results showed that the HMGB1–KGN microsphere can promote cell proliferation via recruiting rat bone marrow stem cells(BMSCs)and activating the BMSCs from G_(0) to G_(Alert) stage as evidenced by cell migration testing and 5-bromo-2′-deoxyuridine(BrdU)incorporation *** vivo results indicated that this HMGB-KGN microsphere can recruit GFP-labeled BMSCs from tail vein to wounded meniscus and induce these GFP-labeled BMSCs to differentiate into *** results demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus healing in vivo.