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Effect of Silicon Amendment on Growth and Nitrogen Status of Common Landscaping Plants

American Journal of Plant Sciences 2024年第8期15卷 603-616页

作者： Bárbara Nogueira Souza Costa Milagros Ninoska Munoz Salas kylie Gil Amir Ali Khoddamzadeh Department of Earth and Environment Institute of Environment Florida International University Miami FL USA

Agriculture and natural vegetations in South Florida face with significant environmental threats such as heat and saltwater intrusion. This study aimed to investigate how silicon application could improve growth parameters and plant health of landscaping plants under extreme temperatures, influenced by global climate changes. Cocoplum (Chrysobalanus icaco), cabbage palm (Sabal palmetto), satinleaf (Chrysophyllum oliviforme), and wild coffee (Psychotria nervosa) plants received an initial slow-release fertilizer of 15 g/pot with an 8N-3P-9K composition. Silicon was applied as a 1% silicic acid solution, with concentrations ranging from 0 g/pot to 6 g/pot of 7.5 L. Evaluations were carried out every 30 days, continuing until 180 days after the treatment was completed. Phenotypic traits, including leaf count and plant height, were assessed alongside measurements from handheld optical non-destructive sensors. These measurements included the normalized difference vegetation index (NDVI), SPAD-502, and atLEAF chlorophyll meters. Application of 4 g/pot and 6 g/pot of silicon significantly improved NDVI values (0.78). Conversely, cocoplum plants exhibited greater plant height (79.6) at 0 g/pot silicon compared to other treatments. In wild coffee samplings, the control group showed the highest plant height and SPAD readings (93.49) compared to other treatments. Interestingly, the control treatment also demonstrated a superior atLEAF value as compared to other treatments, while the tallest samplings were observed with 6 g/pot of silicon (62.82) in cabbage palm plants. The findings indicate that silicon application positively influenced plant growth, particularly evident in cabbage palms. However, cocoplum and wild coffee exhibited a negative correlation between plant height and silicon concentrations.

关键词： Silicon Application Chlorophyll Content Cocoplum Cabbage Palm Satinleaf Wild Coffee

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Genome reprogramming for synthetic biology

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Frontiers of Chemical Science and Engineering 2017年第1期11卷 37-45页

作者： kylie Standage-Beier Xiao Wang School of Life Sciences Arizona State University Tempe AZ 85287 USA School of Biological and Health Systems Engineering Arizona State University Tempe AZ 85287 USA

The ability to go from a digitized DNA sequence to a predictable biological function is central to synthetic biology. Genome engineering tools facilitate rewriting and implementation of engineered DNA sequences. Recent development of new programmable tools to reengineer genomes has spurred myriad advances in synthetic biology. Tools such as clustered regularly interspace short palindromic repeats enable RNA-guided rational redesign of organisms and implementation of synthetic gene systems. New directed evolution methods generate organisms with radically restructured genomes. These restructured organisms have useful new phenotypes for biotechnology, such as bacteriophage resistance and increased genetic stability. Advanced DNA synthesis and assembly methods have also enabled the construction of fully synthetic organisms, such as J. Craig Venter Institute （JCVI）-syn 3.0. Here we summarize the recent advances in programmable genome engineering tools.

关键词： CRISPR genome engineering syntheticbiology rational design

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Bystanders or real players:virtual memory T cells keep chronic infections in check

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Cellular & Molecular Immunology 2020年第8期17卷 797-798页

作者： kylie M.Quinn Tabinda Hussain School of Health and Biomedical Sciences RMIT UniversityBundooraVIC3083Australia Department of Biochemistry and Molecular Biology Monash UniversityClaytonVIC3800Australia Monash Biomedicine Discovery InstituteMonash UniversityClaytonVIC3800Australia Department of Biochemistry and Molecular Biology Monash UniversityClaytonVIC3800Australia

Jin et *** that a recently defined T-cell population,known as virtual memory T cells,accumulate with human immunodeficiency virus(HIV),are inversely correlated with latent viral load and may be uniquely well suited to eliminate latently infected cells during chronic *** with HIV presents many challenges to the immune system,but viral reservoirs are one of the most *** of HIV has been revolutionized by antiretroviral therapy(ART),which effectively ablates viral replication in ***,a real cure remains elusive,as HIV will persist in latently infected *** identification of immune mechanisms that can reduce this latent reservoir may improve treatment and progress cure strategies for *** this issue,Jin et *** that the activity of an unconventional immune cell,the virtual memory T(TVM)cell,may play a role.

关键词： infections latent reservoir

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Therapeutics involved in managing initial and recurrent Clostridium difficile infection:An updated literature review

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2024年第5期15卷 1-14页

作者： Vignesh K Nagesh Hadrian Hoang-Vu Tran Daniel Elias Izage Kianifar Aguilar Tanni Sethi AiswaryaMenon Charlene Mansour Florchi Furman kylie Tsotsos Talia Subar Auda Auda Aman Sidiqui JevonLamar Nikita Wadhwani Shraboni Dey Abraham Lo Adam Atoot Simcha Weissman Humberto Sifuentes Ayrton I Bangolo Department of Internal Medicine Hackensack Palisades Medical CenterNorth BergenNJ 07047United States Department of Gastroenterology Augusta UniversityAugustaGA 30912United States

Clostridium difficile infection(CDI)has been increasing due to the effect of recurrent *** use of antibiotics has been shown to alter the gut microbiome and lead to *** treatment is limited to three major antibiotics;however,the incidence of recurrent CDIs has been increasing and drug resistance is a major *** aspect is a growing concern in modern medicine especially in the elderly population,critical care patients,and immunocompromised individuals who are at high risk of developing *** difficile can lead to various complications including septic shock and fulminant colitis that could prove to be lethal in these *** modalities of treatment have been developed including bezlotoxumab,a monoclonal antibody and fecal microbiota *** have been studies showing asymptomatic carriers and drug resistance posing a major threat to the healthcare *** treatment options are being studied to treat and prevent *** review will provide an insight into the current treatment modalities,prevention and newer modalities of treatment and challenges faced in the treatment of CDIs.

关键词： Clostridium difficile Antibiotics Vancomycin Fidaxomicin Prevention Bezlotoxumab Fecal microbiota transplant

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Design and fabrication of alumina micro reciprocating engine

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Journal of Harbin Institute of Technology(New Series) 2008年第6期15卷 801-804页

作者：金鹏高育龙刘楠 JIANG kylie Institute of Ultra-precision Optoelectronic Instrument Engineering Harbin Institute of Technology School of Engineering the University of Birmingham

Microengines are regarded as the potential replacements for *** at the unsatisfactory thermal property of silicon used in the combustion chambers,ceramics are chosen as the construction material in this *** fabrication process where alumina has been chosen as the microengine material is *** FEA has been carried out,and it is found that the material satisfies the stress and deformation requirements of the *** the alumina fabrication process is *** reusable polydimethylsiloxane(PDMS)moulds are produced from SU-8 resist masters,and alumina microengine parts are produced using the PDMS *** of the ceramic components show that the fabrication satisfies the design requirements.

关键词： micro reciprocating engine alumina slurry replicating process SU-8 finite element analysis (FEA) soft reusable polydimethylsiloxane (PDMS) moulds

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Sexuality following trauma injury: A literature review

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Burns & Trauma 2014年第2期2卷 61-70页

作者： kylie Marie Connell Rosemary Coates Fiona Melanie Wood Department of Sexology School of Public HealthFaculty of Health SciencesCurtin UniversityPerthWestern Australia Fiona Wood Foundation PerthWestern Australia Burns Service of Western Australia Royal Perth HospitalWestern Australia4Burn Injury Research Unit University of Western Australia PerthWestern AustraliaAustralia

Restoration of the quality of life(QoL)of trauma injury survivors is the aim of trauma *** is generally acknowledged that sexuality is an important component of QoL;however,rehabilitation services frequently fall short of including sexuality as a matter of *** literature was reviewed to examine the experiences of trauma survivors from three groups:spinal cord injury(SCI),traumatic brain injury(TBI)and *** focus was on the impact of trauma on the QoL to identify future research directions and to advocate for the inclusion of sexuality as an integral part of *** searched were Proquest,Ovid,Cinahl,Medline,PsycInfo and Cochrane Central Register of controlled trials.A total of 36 eligible studies were included:SCI(n=25),TBI(n=6),burns(n=5).Four themes were identified across the three trauma groups that were labeled as physiological impact of trauma on sexuality,cognitive-genital dissociation(CGD),sexual disenfranchisement(SD)and sexual rediscovery(SR).Trauma injury has a significant impact on sexuality,which is not routinely addressed within rehabilitation *** sexuality research is required among all trauma groups to improve rehabilitation services and in turn QoL outcomes for all trauma survivors.

关键词： Burns rehabilitation spinal cord injury sexuality traumatic brain injury

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Design and operando/in situ characterization of precious-metal-free electrocatalysts for alkaline water splitting

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Carbon Energy 2020年第4期2卷 582-613页

作者： Tingwen Zhao Yuan Wang Siva Karuturi kylie Catchpole Qiang Zhang Chuan Zhao School of Chemistry Faculty of ScienceThe University of New South WalesSydneyNew South WalesAustralia Research School of Electrical Energy and Materials EngineeringThe Australian National UniversityCanberraAustralian Capital TerritoryAustralia Department of Chemical Engineering Tsinghua UniversityBeijingChina

Electrochemical water splitting has attracted considerable attention for the production of hydrogen fuel by using renewable energy ***,the sluggish reaction kinetics make it essential to explore precious-metal-free electrocatalysts with superior activity and long-term *** efforts have been made in exploring electrocatalysts to reduce the energy barriers and improve catalytic *** review summarizes different categories of precious-metal-free electrocatalysts developed in the past 5 years for alkaline water *** design strategies for optimizing the electronic and geometric structures of electrocatalysts with enhanced catalytic performance are discussed,including composition modulation,defect engineering,and structural ***,the advancement of operando/in situ characterization techniques toward the understanding of structural evolution,reaction intermediates,and active sites during the water splitting process are ***,current challenges and future perspectives toward achieving efficient catalyst systems for industrial applications are *** review will provide insights and strategies to the design of precious-metalfree electrocatalysts and inspire future research in alkaline water splitting.

关键词： alkaline water splitting catalysts design electrocatalysts operando/in situ characterization precious-metal-free catalysts

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Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

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Light(Science & Applications) 2022年第11期11卷 2790-2804页

作者： Philip Wijesinghe Stella Corsetti Darren J.X.Chow Shuzo Sakata kylie R.Dunning Kishan Dholakia SUPA School of Physics and AstronomyUniversity of St AndrewsNorth HaughSt AndrewsFife KY169SSUK Robinson Research Institute Adelaide Medical SchoolThe University of AdelaideAdelaideSAAustralia Australian Research Council Centre of Excellence for Nanoscale BioPhotonics The University of AdelaideAdelaideSAAustralia Institute for Photonics and Advanced Sensing The University of AdelaideAdelaideSAAustralia Strathclyde Institute of Pharmacy and Biomedical Sciences University of StrathclydeGlasgow G4 OREUK School of Biological Sciences The University of AdelaideAdelaideSAAustralia Department of Physics College of ScienceYonsei UniversitySeoul 03722South Korea

Deconvolution is a challenging inverse problem,particularly in techniques that employ complex engineered pointspread functions,such as microscopy with propagation-invariant ***,we present a deep-learning method for deconvolution that,in lieu of end-to-end training with ground truths,is trained using known physics of the imaging ***,we train a generative adversarial network with images generated with the known point-spread function of the system,and combine this with unpaired experimental data that preserve perceptual *** method rapidly and robustly deconvolves and super-resolves microscopy images,demonstrating a two-fold improvement in image contrast to conventional deconvolution *** contrast to common end-to-end networks that often require 1000-10,000s paired images,our method is experimentally unsupervised and can be trained solely on a few hundred regions of *** demonstrate its performance on light-sheet microscopy with propagation-invariant Airy beams in oocytes,preimplantation embryos and excised brain tissue,as well as illustrate its utility for Bessel-beam *** method aims to democratise learned methods for deconvolution,as it does not require data acquisition outwith the conventional imaging protocol.

关键词： invariant propagation implantation

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A comparison of handheld and standard ultrasound in Swiss medical students

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World Journal of Emergency Medicine 2022年第2期13卷 85-90页

作者： Mark Slader Hayley Young Margot Barker kylie Prentice Katherine Bogaard Charlene Yuan Soheil Saadat Shadi Lahham School of Medicine University of CaliforniaIrvine 92617USA Department of Emergency Medicine University of CaliforniaIrvine 92868USA

BACKGROUND:The use of ultrasound(US)within healthcare has inspired the development of new US *** have been few studies comparing the use of handheld US to standard US for medical *** research aims to determine whether a handheld US device can provide a comparable primary learning experience to that of a standard US ***:Over two days of instruction,participants were taught and evaluated on core US *** standard group received instruction on standard US machines,while the handheld group received instruction on handheld US *** completed a qualitative survey regarding their *** hundred and four images were obtained and graded by two emergency medicine ***:A total of 119 Swiss medical students were enrolled in our *** was no statistically significant difference in the US assessment measurements,except for faster endpoint septal separation(EPSS)vascular setup time in the handheld group(P=0.001).There was no statistically significant difference in participants’perceived difficulty of US learning(P=0.198),comfort level(P=0.188),or self-estimated capability to perform US in the future(P=0.442).There was no statistically significant difference in the percentage of correctly obtained images(P=0.211)or images that were clinically useful(P=0.256).The median quality score of images obtained by the standard group was eight compared to seven in handheld group(P<0.01).CONCLUSION:Our data suggest a handheld US machine can perform as well as a standard US machine as an educational tool despite sacrifices in image quality.

关键词： Handheld ultrasound Ultrasound education Switzerland Medical education

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Symmetry-aware recursive image similarity exploration for materials microscopy

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npj Computational Materials 2021年第1期7卷 1508-1521页

作者： Tri N.M.Nguyen Yichen Guo Shuyu Qin kylie S.Frew Ruijuan Xu Joshua C.Agar Department of Materials Science and Engineering Lehigh UniversityBethlehemPA 18015USA Department of Computer Science and Engineering Lehigh UniversityBethlehemPA 18015USA Department of Mechanical Engineering Lehigh UniversityBethlehemPA 18015USA Department of Applied Physics Stanford UniversityStanfordCA 94305USA

In pursuit of scientific discovery,vast collections of unstructured structural and functional images are acquired;however,only an infinitesimally small fraction of this data is rigorously analyzed,with an even smaller fraction ever being *** method to accelerate scientific discovery is to extract more insight from costly scientific experiments already ***,data from scientific experiments tend only to be accessible by the originator who knows the experiments and ***,there are no robust methods to search unstructured databases of images to deduce correlations and ***,we develop a machine learning approach to create image similarity projections to search unstructured image *** improve these projections,we develop and train a model to include symmetry-aware *** an exemplar,we use a set of 25,133 piezoresponse force microscopy images collected on diverse materials systems over five *** demonstrate how this tool can be used for interactive recursive image searching and exploration,highlighting structural similarities at various length *** tool justifies continued investment in federated scientific databases with standardized metadata schemas where the combination of filtering and recursive interactive searching can uncover synthesis-structure-property *** provide a customizable open-source package(https://***/m3-learning/Recursive_Symmetry_Aware_Materials_Microstructure_Explorer)of this interactive tool for researchers to use with their data.

关键词： interactive image similarity

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