检索结果-南通市图书馆

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim kyeong-hyeon eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation

引用

畜牧与生物技术杂志(英文版) 2024年第1期

作者： Gyeong-Min Gim kyeong-hyeon eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

Highly Elastic,Bioresorbable Polymeric Materials for Stretchable,Transient Electronic Systems

引用

Nano-Micro Letters 2024年第6期16卷 1-13页

作者： Jeong‑Woong Shin Dong‑Je Kim Tae‑Min Jang Won Bae Han Joong Hoon Lee Gwan‑Jin Ko Seung Min Yang Kaveti Rajaram Sungkeun Han Heeseok Kang Jun hyeon Lim Chan‑Hwi eom Amay J.Bandodkar Hanul Min Suk‑Won Hwang KU‑KIST Graduate School of Converging Science and Technology Korea University145 Anam‑roSeongbuk‑guSeoul 02841Republic of Korea SK Hynix 2091Gyeongchung‑daeroBubal‑eupIcheon‑siGyeonggi‑do 17336Republic of Korea Hanwha Systems Co. Ltd.188Pangyoyeok‑roBundang‑guSeongnam‑siGyeonggi‑do 13524Republic of Korea Department of Electrical and Computer Engineering North Carolina State UniversityRaleighNC 27606USA Center for Advanced Self‑Powered Systems of Integrated Sensors and Technologies(ASSIST) North Carolina State UniversityRaleighNC 27606USA Department of Integrative Energy Engineering Korea University145 Anam‑roSeongbuk‑guSeoul 02841Republic of Korea Biomaterials Research Center Korea Institute of Science and Technology(KIST)5 Hwarang‑ro 14‑gilSeongbuk‑guSeoul 02792Republic of Korea Semiconductor R&D Center Samsung Electronics Co.Ltd.Hwaseong‑siGyeonggi‑do 18448Republic of Korea Center for Advanced Biomolecular Recognition Biomedical Research DivisionKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea

Substrates or encapsulants in soft and stretchable formats are key components for transient,bioresorbable electronic systems;however,elastomeric polymers with desired mechanical and biochemical properties are very limited compared to nontransient ***,we introduce a bioresorbable elastomer,poly(glycolide-co-ε-caprolactone)(PGCL),that contains excellent material properties including high elongation-at-break(<1300%),resilience and toughness,and tunable dissolution *** of PGCLs as polymer matrices,in combination with conducing polymers,yields stretchable,conductive composites for degradable interconnects,sensors,and actuators,which can reliably function under external *** of device components with wireless modules demonstrates elastic,transient electronic suture system with on-demand drug delivery for rapid recovery of postsurgical wounds in soft,time-dynamic tissues.

关键词： Biodegradable elastomer Conductive polymer composites Biomedical device Transient electronics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Submicron Ag-coated Cu particles as filler in sintering paste: Suppression of Ag dewetting and improvement of sinterability by surface modification using stearic acid

引用

Transactions of Nonferrous Metals Society of China 2024年

作者： Yeongjung Kim Yong-Sung eom Kwang-Seong Choi Jong-Hyun Lee Department of Materials Science and Engineering Seoul National University of Science and Technology 232 Gongneung-ro Low-Carbon Integration Tech Creative Research Section ETRI 218 Gajeong-ro Materials Research Institute for Future Convergence Seoul National University of Science and Technology 232 Gongneung-ro

Four types of submicron Ag-coated Cu particles with different Ag contents were prepared as sintering paste fillers， and the Ag contents of the particles were measured to be 10， 20， 30， and 40 wt%. Four types of particles （in order of increasing Ag content: A10， A20， A30， and A40） were surface-modified with stearic acid， to suppress the Ag shell dewetting and improve sinterability. The surface-modified particles were mixed with a polyol-based solvent to fabricate a resin-free paste. Subsequently， the pastes were screen-printed onto a slide glass and sintered at 250 ℃ in a nitrogen atmosphere for 1–10 min to form an electrode. The electrical resistivity of the sintered film as a function of sintering time was measured using a four-point probe. All the four surface-modified Cu@Ag particles with different Ag contents exhibited decreased electrical resistivity. Particularly， the largest difference in values after and before the surface modification was observed for A40 with the highest Ag content; the electrical resistivities of the initial and surface-modified particles were 1.51×10-4and 6.67×10-5Ω·cm， respectively， after sintering for 10 min. The findings of this study confirmed that the surface modification using stearic acid effectively suppressed the dewetting of the Ag shell and improved the sinterability of the submicron Cu@Ag particles.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Publisher Correction to:Highly Elastic,Bioresorbable Polymeric Materials for Stretchable,Transient Electronic Systems

引用

Nano-Micro Letters 2024年第8期16卷 417-417页

作者： Jeong‑Woong Shin Dong‑Je Kim Tae‑Min Jang Won Bae Han Joong Hoon Lee Gwan‑Jin Ko Seung Min Yang Kaveti Rajaram Sungkeun Han Heeseok Kang Jun hyeon Lim Chan‑Hwi eom Amay JBandodkar Hanul Min Suk‑Won Hwang KU‑KIST Graduate School of Converging Science and Technology Korea University145 Anam‑roSeongbuk‑guSeoul 02841Republic of Korea SK Hynix 2091Gyeongchung‑daeroBubal‑eupIcheon‑siGyeonggi‑do 17336Republic of Korea Hanwha Systems Co. Ltd.188Pangyoyeok‑roBundang‑guSeongnam‑siGyeonggi‑do 13524Republic of Korea Department of Electrical and Computer Engineering North Carolina State UniversityRaleighNC 27606USA Center for Advanced Self‑Powered Systems of Integrated Sensors and Technologies(ASSIST) North Carolina State UniversityRaleighNC 27606USA Department of Integrative Energy Engineering Korea University145 Anam‑roSeongbuk‑guSeoul 02841Republic of Korea Biomaterials Research Center Korea Institute of Science and Technology(KIST)5 Hwarang‑ro 14‑gilSeongbuk‑guSeoul 02792Republic of Korea Semiconductor R&D Center Samsung Electronics Co.Ltd.Hwaseong‑siGyeonggi‑do 18448Republic of Korea Center for Advanced Biomolecular Recognition Biomedical Research DivisionKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea

Due to typesetting mistake,Hanul Min was missed to be denoted as a corresponding author in the *** type-setter apologizes for *** original article has been *** Access This article is licensed under a Creative Commons Attribution 4.0 International License,which permits use,sharing,adaptation,distribution and reproduction in any medium or format,as long as you give appropriate credit to the original author(s)and the source,provide a link to the Creative Commons licence,and indicate if changes were *** images or other third party material in this article are included in the article’s Creative Commons licence,unless indicated otherwise in a credit line to the *** material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use,you will need to obtain permission directly from the copyright holder.

关键词： Creative Elastic Highly

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Automated X-ray Defect Inspection on Occluded BGA Balls Using Hybrid Algorithm

引用

Computers, Materials & Continua 2023年第6期75卷 6337-6350页

作者： Ki-Yeol eom Byungseok Min Department of AI Bigdata Suseong UniversityDaegu42078Korea R&D Center XAVIS Co.Ltd.Seongnam-si13202Korea

Automated X-ray defect inspection of occluded objects has been an essential topic in semiconductors,autonomous vehicles,and artificial intelligence ***,there are few solutions to segment occluded objects in the X-ray inspection *** particular,in the Ball Grid Array inspection of X-ray images,it is difficult to accurately segment the regions of occluded solder balls and detect defects inside solder *** this paper,we present a novel automatic inspection algorithm that segments solder balls,and detects defects fast and efficiently when solder balls are *** proposed algorithm consists of two *** the first stage,the defective candidates or defects are determined through the following four steps:(i)image preprocessing such as noise removal,contrast enhancement,binarization,connected component,and morphology,(ii)limiting the inspec-tion area to the ball regions and determining if the ball regions are occluded,(iii)segmenting each ball region into one or more regions with similar gray values,and(iv)determining whether there are defects or defective candidates in the regions using a weighted sum of local threshold on local *** there are defective candidates,the determination of defects is finally made in the following *** the second stage,defects are detected using the automated inspection technique based on oblique computed *** 3D precision inspection process is divided into four steps:(i)obtaining 360 projection images(one image per degree)rotating the object from 0 to 360 degrees,(ii)reconstructing a 3D image from the 360 projected images,(iii)finding the center slice of gravity for solder balls from the axial slice images in the z-direction,and getting the inspection intervals between the upper bound and the lower bound from the center slice,and(iv)finally determining whether there are defects in the averaged image of solder *** proposed hybrid algorithm is robust for segmenting the defects inside o

关键词： Hybrid voids BGA X-ray inspection defects

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcriptomic Responses of Garlic(Allium sativum L.)to Heat and Drought Stresses

引用

Phyton-International Journal of Experimental Botany 2023年第11期92卷 3077-3090页

作者： Seung Hee eom Tae Kyung Hyun Department of Industrial Plant Science and Technology Chungbuk National UniversityCheongju28644Korea

Heat and drought are prominent abiotic stressors that limit crop productivity and yield,particularly concerning climate change;therefore,understanding the molecular mechanisms underlying plant stress responses is crucial for stress-tolerant crop *** study conducted a transcriptomic analysis to elucidate how garlic(Allium sativum L.)responds to drought and heat stress *** libraries were generated to identify differentially expressed genes(DEGs)induced by drought and heat *** classification and clustering analysis of DEGs revealed stress-specific gene expression ***,cell wall-related genes were implicated in the drought response,whereas heat stress was associated with heat stress transcription factors and heat shock *** results provide essential information for future studies on stress tolerances in garlic.

关键词： Differentially expressed gene drought heat garlic transcriptome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Thoracic duct cannulation during left internal jugular vein cannulation:A case report

引用

World Journal of Clinical Cases 2023年第34期11卷 8200-8204页

作者： Geal Hong Hwang Woosik eom Department of Anesthesiology and Pain Medicine National Cancer CenterGoyang-si 10408Gyeonggi-doSouth Korea

BACKGROUND Central venous catheter insertion is an invasive procedure that can cause complications such as infection,embolization due to air or blood clots,pneumothorax,hemothorax,and,rarely,chylothorax due to damage to the thoracic ***,we report a case of suspected thoracic duct cannulation that occurred during left central venous catheter ***,the patient was discharged without any adverse events related to thoracic duct *** SUMMARY A 46-year-old female patient presented at our department to undergo cytoreductive surgery and hyperthermic intraperitoneal *** anesthesia,we decided to insert a central venous catheter through the left internal jugular vein because the patient already had a chemoport through the right central *** the procedure,blood reflux was observed when the needle tip was not within the ultrasound field of *** did not try to find the tip;however,a guide wire and a central venous catheter were inserted without any ***,when inducing blood reflux from the distal port of the central venous catheter,only clear fluid,suspected to be lymphatic fluid,was ***,chest X-ray revealed an appearance similar to that of the path of the thoracic *** that intravenous fluid administration was not started and no abnormal fluid collection was noted on preoperative chest X-ray,we suspected thoracic duct *** It is important to use ultrasound to confirm the exact position of the needle tip and guide wire path.

关键词： Central venous catheter insertion Left internal jugular vein Thoracic duct Lymph Ultrasound Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of preparation method of noble metal supported catalyts on formaldehyde oxidation at room temperature: Gas or liquid phase reduction

引用

Journal of Environmental Sciences 2022年第12期34卷 201-216页

作者： Younghee Jang Ye Hwan Lee Hanki eom Sang Moon Lee Sung Su Kim Department of Environmental Energy Envineering Graduate School of kyonggi UniversityGyeonggi-do 16227Korea Department of Environmental Energy Engineeing Kyonggi UniversityGyonggi-do 16227Korea

Formaldehyde(HCHO) is toxic to the human body and is one of the main threats to the indoor air quality(IAQ). As such, the removal of HCHO is imperative to improving the IAQ,whereby the most useful method to effectively remove HCHO at room temperature is catalytic oxidation. This review discusses catalysts for HCHO room-temperature oxidation,which are categorized according to their preparation methods, i.e., gas-phase reduction and liquid-phase reduction methods. The HCHO oxidation performances, structural features,and reaction mechanisms of the different catalysts are discussed, and directions for future research on catalytic oxidation are reviewed.

关键词： Formaldehyde Indoor air quality Catalyst Prepared method

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Origin of the giant persistent photoconductivity in LaAlO_(3)/SrTiO_(3)heterostructures probed by noise spectroscopy

引用

Journal of Materials Science & Technology 2023年第6期137卷 152-158页

作者： Kitae eom Jung-Woo Lee Gyeongmo Yang Youngmin Kim Jaeyoung Jeon Jieun Yeon Hyungwoo Lee School of Advanced Materials Science and Engineering Sungkyunkwan University(SKKU)Suwon 16419South Korea KIURI Institute Yonsei UniversitySeoul 03722South Korea Department of Physics Ajou UniversitySuwon 16499South Korea Department of Energy Systems Research Ajou UniversitySuwon 16499South Korea

LaAlO_(3)/SrTiO_(3)(LAO/STO)heterostructures have shown a strong persistent photoconductivity(PPC)at room *** abnormally strong PPC has attracted immense research interest due to its possible applications in optically-tunable electronic *** its promise,the fundamental understanding of the PPC in the LAO/STO heterostructures is still ***,we report that the giant PPC origi-nates from the photo-induced valence change in oxygen vacancies near the LAO/STO *** spec-tral analysis of the photocurrent and the model-fitting study consistently show that the ionized oxygen vacancies near the interface are neutralized during the electron relaxation *** hinder the complete relaxation of the photoexcited electrons by the deeply-located oxygen vacancies and result in the strong *** change in the ionization state distribution of the oxygen vacancies is probed by the per-sistent noise behavior at the frequency between 1 kHz and 20 kHz *** results provide insight into the role of oxygen vacancies in influencing the internal charge distribution and triggering the PPC phenomena in complex oxide heterostructures.

关键词： Persistent photoconductivity LaAlO_(3)/SrTiO_(3)heterostructures Two-dimensional electron gases Oxygen vacancies Low-frequency noise Spectral analyses

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：