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A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stress

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Journal of Integrative Plant Biology 2012年第9期54卷 640-651页

作者： Jihyun Je Chieun Song Jung Eun Hwang chae Oh Lim Systems and Synthetic Agrobiotech Center and PMBBRC Division of Applied Life Science Gyeongsang National University

The dehydration-responsive element-binding factor 2C （DREB2C） is a member of the CBF/DREB subfamily of proteins, which contains a single APETALA2/Ethylene responsive element-binding factor （AP2/ERF） domain. To identify the expression pattern of the DREB2C gene, which contains multiple transcription cis-regulatory elements in its promoter, an approximately 1.4 kb upstream DREB2C sequence was fused to the β-glucuronidase reporter gene （GUS） and the recombinant p1244 construct was transformed into Arabidopsis thaliana （L.） Heynh. The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues. Upon applying heat stress （HS）, GUS staining was also enhanced in the vasculature of the growing tissues. Analysis of a series of 5＇-deletions of the DREB2C promoter revealed that a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between -204 and -34 bps relative to the transcriptional start site. Furthermore, electrophoretic mobility shift assay （EMSA） demonstrated that nuclear protein binding activities specific to a -120 to -32 bp promoter fragment increased after HS. These results indicate that the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter.

关键词： cis-acting elements β-glucuronidase gene expression histochemical assay promoter deletion transgenic plant.

Upstream Channel Utilization of CSMA/CD-Based Ethernet over Passive Optical Networks

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光学学报 2003年第S1期23卷 737-738页

作者： Elaine Wong Chang-Joon chae Australian Photonics Cooperation Research Centre Photonics Research Laboratory Department of Electrical and Electronic Engineering The University of Melbourne VIC 3010 Australia.Australian Photonics Cooperation Research Centre Photonics Research Laboratory Department of Electrical and Electronic Engineering The University of Melbourne VIC 3010 Australia.

We demonstrate that an asynchronous CSMA/CD protocol with an optical loop-back star coupler can achieve high upstream channel utilizatbn in an Ethernet PON, readily supporting a large installed base of Ethernet 10Base... 详细信息

关键词： CSMA on as of Upstream Channel Utilization of CSMA/CD-Based Ethernet over Passive Optical Networks ONU OLT over CD

Effects of Momentum Ratio and Weber Number on Spray Half Angles of Liquid Controlled Pintle Injector

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Journal of Thermal Science 2015年第1期24卷 37-43页

作者： Min Son Kijeong Yu Jaye Koo Oh chae Kwon Jeong Soo Kim Korea Aerospace University School of Aerospace and Mechanical Engineering Korea Aerospace University School of Mechanical Engineering Sungkyunkwan University Department of Mechanical Engineering Pukyong National University

A pintle injector is advantageous for throttling a liquid rocket engine and reducing engine weight. This study ex- plores the effects of momentum ratio and Weber number at various injection conditions on spray characteristics of the pintle injector for liquid-gas propellants. A liquid sheet is injected from a center pintle nozzle and it is broken by a gas jet from an annular gap. The pressure drops of propellants, and the pintle opening distance were consi- dered as control variables; using 0.1 -1.0 as a bar for the pressure drop of the liquid injection, a 0.01-4）.2 bar for the pressure drop of gas jet and a 0.2 - 1.0 mm for the pintle opening distance. The discharge coefficient was de- creased linearly before the pintle opening distance of 0.75 mm and then, the coefficient was slightly increased. Spray images were captured by a CMOS camera with high resolution. Then, the shadow and reflected images were analyzed. Spray distributions were measured by a pattemator with an axial distance of 50 mm from a pintle tip. Finally, the spray half angles had an exponentially decreasing correlation as a momentum ratio divided by the Weber number. Also, the spray half angles from the spray distribution were underestimated compared to those measured from the captured images.

关键词： Pintle Injector Spray Half Angle Spray Distribution Visualization

Effects of local compositional and structural disorder on vacancy formation in entropy-stabilized oxides from first-principles

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npj Computational Materials 2022年第1期8卷 909-915页

作者： Sieun chae Logan Williams Jihang Lee John T.Heron Emmanouil Kioupakis Department of Materials Science and Engineering University of MichiganAnn ArborMIUSA

Entropic stabilization has evolved into a strategy to create new oxide materials and realize novel functional properties engineered through the alloy *** an atomistic understanding of these properties to enable their design,however,has been challenging due to the local compositional and structural disorder that underlies their fundamental structure-property ***,we combine high-throughput atomistic calculations and linear regression algorithms to investigate the role of local configurational and structural disorder on the thermodynamics of vacancy formation in (MgCoNiCuZn)O-based entropy-stabilized oxides (ESOs) and their influence on the electrical *** find that the cation-vacancy formation energies decrease with increasing local tensile strain caused by the deviation of the bond lengths in ESOs from the equilibrium bond length in the binary *** oxygen-vacancy formation strongly depends on structural distortions associated with the local configuration of chemical *** in ESOs exhibit deep thermodynamic transition levels that inhibit electrical *** applying the charge-neutrality condition,we determine that the equilibrium concentrations of both oxygen and cation vacancies increase with increasing Cu mole *** results demonstrate that tuning the local chemistry and associated structural distortions by varying alloy composition acts an engineering principle that enables controlled defect formation in multi-component alloys.

关键词： alloy structural vacancy

Enhanced electrochemical performance of graphitic carbon-wrapped spherical FeOF nanoparticles using maleopimaric acid as a cathode material for sodium-ion batteries

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Journal of Materials Science & Technology 2021年第26期85卷 184-193页

作者： Achmad Yanuar Maulana Jungwook Song Da Won Leeg chae Eun Leeg Jongsik Kimg Department of Chemistry Dong-A UniversityBusan 49315South Korea Department of Chemical Engineering(BK21 FOUR Graduate Program) Dong-A UniversityBusan 49315South Korea

Sodium-ion batteries(SIBs)are considered one of the most promising energy storage systems for replacing lithium-ion batteries because of the high abundance and low cost of *** oxyfluoride(FeOF)is a promising conversion-based cathode material for SIBs because of its high theoretical capacity of about855 mA h g^(-1),low-cost chemical compositions,and its lower sensitivity to the size of charged carrier ***,the poor electrical conductivity and ionic diffusion of FeOF result in a low rate capability and *** this work,FeOF nanoparticles wrapped by graphitic carbon layers were synthesized using abietic or maleopimaric acid as both the carbon source and organic *** addition,the morphology of the FeOF particles was gradually controlled from rod to spherical shapes,simply depending on the rosin *** FeOF nanoparticles prepared with maleopimaric acid showed a large reversible discharge capacity of 356.7 mA h g^(-1)with a fading rate of 0.21%per cycle after 100 cycles at a current density of 100 mA g^(-1)and an excellent rate capability.

关键词： Na-ion batteries Cathode Iron oxyfluoride Graphitized carbon Maleopimaric acid

Impact of statin intensity on adverse cardiac and cerebrovascular events in older adult patients with myocardial infarction

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Journal of Geriatric Cardiology 2021年第8期18卷 609-622页

作者： In Tae Moon Si-Hyuck Kang Wonjae Lee Youngjin Cho Jin Joo Park Yeonyee EYoon Il-Young Oh Chang-Hwan Yoon Jung-Won Suh Tae-Jin Youn In-Ho chae Dong-Ju Choi Young-seok Cho Uijeongbu Eulji Medical Center Eulji University School of MedicineUijeongbuSouth Korea Seoul National University Bundang Hospital SeongnamSouth Korea Department of Cardiology Incheon Sejong HospitalIncheonSouth Korea

BACKGROUND There is insufficient evidence regarding the effect of high-intensity statin therapy in older *** study aimed to investigate the effects of high-intensity statin treatment on the clinical outcomes in older adults with myocardial infarc-tion(MI).METHODS Consecutive patients with MI aged at least 75 years were analyzed *** primary endpoint was major adverse cardiac and cerebrovascular events(MACCE),defined as a composite of all-cause death,MI,rehospitalization due to un-stable angina,repeat revascularization,and ischemic *** high-intensity group was compared to the low-to-moderate in-tensity group in the propensity score-matched *** Average age of total 546 patients was 81 *** them,84%of patients underwent percutaneous coronary *** unadjusted seven-year MACCE rate differed by statin intensity(high-intensity statin group:38%,moderate-intensity statin group:42%,low-intensity statin group:56%,and no-statin group:61%,P=0.004).However,among these groups,many baseline characteristics were significantly *** the 74 propensity score-matched pairs,which lacked any significant differences in all baseline characteristics,the high-intensity group had a significantly lower rate of MACCE than the low-to-moderate intensity group(37%vs.53%,P=0.047).Follow-up low-density lipoprotein cholesterol levels were significantly lower in the high-intensity group than that in the low-to-moderate intensity group(69.4±16.0 mg/dL vs.77.9±25.9 mg/dL,P=0.026).CONCLUSIONS In older adult patients with MI,the use of high-intensity statin caused significantly less occurrence of MACCE in comparison to that in low-to-moderate intensity for up to seven years of follow-up.

关键词： patients cardiac intensity

Extragastrointestinal stromal tumor presenting as a scrotal mass： an unusual case

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Asian Journal of Andrology 2007年第2期9卷 275-279页

作者： seok-Ho Kang Myung-Joon Kim Min-Gu Park Hong-seok Park Du-Geon Moon Deuk-Jae Sung Hyun-Chul Kim Yang-seok chae Jun Cheon Je-Jong Kim Department of Urology Korea University School of Medicine Seoul 136-705 Korea Department of Radiology Korea University School of Medicine Seoul 136-705 Korea Department of Pathology Korea University School of Medicine Seoul 136-705 Korea

We describe an unusual case of extragastrointestinal stromal tumor （EGIST） presenting as a scrotal mass. A 71- year-old man presented with a gradually enlarging scrotal mass with a 20-year duration. Physical examination re vealed a huge （as large as volleyball）, round, nontender mass occupying the whole scrotum, which was resected completely. Clinical and radiological findings did not comply with any other primary site disease. Under histological examination, the tumor showed a spindle cell pattern with low cellularity, absence of necrotic and mitotic features. Immunohistochemical anlaysis revealed the tumor reactive for CD117 and CD34, while negative for smooth muscle actin, desmin and S-100 protein. To our knowledge, this is the first reported case of an EGIST involving the scrotum.

关键词： extragastrointestinal stromal tumor scrotum immunohistochemistry

Electroacupuncture for abdominal pain relief in patients with acute pancreatitis:A three-arm randomized controlled trial

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Journal of Integrative Medicine 2023年第6期21卷 537-542页

作者： Dong Kee Jang Jun Kyu Lee Chan Yung Jung Kyung Ho Kim Ha Ra Kang Yeon Sun Lee Jong Hwa Yoon kwang Ro Joo Min Kyu chae Yong Hyeon Baek Byung-Kwan Seo Sang Hyub Lee Chiyeon Lim Department of Internal Medicine Seoul Metropolitan Government Boramae Medical CenterSeoul National University College of MedicineSeoul 07061Republic of Korea Department of Internal Medicine Dongguk University Ilsan HospitalDongguk University College of MedicineGoyang 10326Republic of Korea Department of Acupuncture and Moxibustion Dongguk University Ilsan Oriental HospitalCollege of Korean MedicineDongguk UniversityGoyang 10326Republic of Korea Department of Korean Medicine Dongguk UniversityGoyang 10326Republic of Korea Department of Acupuncture and Moxibustion College of Korean MedicineDongguk UniversityGyeongju 38067Republic of Korea Department of Gastroenterology Kyung Hee University Hospital at GangdongSeoul 05278Republic of Korea Department of Acupuncture and Moxibustion Kyung Hee University Korean Medicine Hospital at GangdongSeoul 05278Republic of Korea Department of Internal Medicine and Liver Research Institute Seoul National University HospitalSeoul National University College of MedicineSeoul 03080Republic of Korea Department of Biostatistics College of MedicineDongguk UniversityGoyang 10326Republic of Korea

Background: Electroacupuncture(EA) may reduce the severity of acute pancreatitis(AP) and provide additional pain relief in patients with chronic pancreatitis. However, the ability of EA to relieve pain in patients with AP has not been well ***: This study was undertaken to compare the pain-relieving effects of EA and conventional treatment in patients with ***, setting, participants and interventions: This study was conducted using a randomized, controlled,three-arm, parallel-group and multi-center design. Patients diagnosed with AP were randomly and equally assigned to EA1, EA2 or control groups. All participants received conventional standard-of-care therapy for AP. Local EA alone was administered in EA1, and local plus distal EA was given in *** EA included two abdominal acupoints, while distal EA included twelve peripheral acupoints. EA groups underwent one session of EA daily for 4 days(days 1–4), or until pain was resolved or *** outcome measures: The primary outcome measure was the change in the visual analogue scale(VAS;0–100) pain score between baseline and day ***: Eighty-nine participants were randomized into EA1, EA2 and control groups, and 88(EA1, 30;EA2, 29;control, 29) were included in the full-analysis set. VAS score change(median [interquartile range]) on day 5 was(12.3 ± 22.5) in the EA1 group,(10.3 ± 21.5) in the EA2 group, and(8.9 ± 15.2) in the control group. There were not significant differences in the change in VAS score among treatments(P = 0.983). However, time to food intake was significantly shorter in the EA group(EA1 + EA2) than in the control group(median 2.0 days vs 3.0 days), with a hazard ratio of 0.581(P = 0.022;95% CI,0.366–0.924). No significant adverse events ***: EA treatment did not significantly reduce pain after 4 days of treatment in patients with AP-associated abdominal pain but significantly reduced time to first food intake.

关键词： Acute pancreatitis Electroacupuncture Pain management Abdominal pain Randomized controlled trial

Challenges for clinicians treating autoimmune pancreatitis:Current perspectives

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World Journal of Clinical Cases 2023年第1期11卷 30-46页

作者： Seong-Hun Kim Yun chae Lee Hyung Ku Chon Division of Gastroenterology Department of Internal MedicineJeonbuk National University Medical SchoolJeonju 54907South Korea Department of Internal Medicine Institution of Wonkwang Medical ScienceWonkwang University School of Medicine and HospitalIksan 54538South Korea

Autoimmune pancreatitis(AIP)is a rare disease clinically characterized by obstructive jaundice,unintentional weight loss,acute pancreatitis,focal pancreatic mass,and *** is classified into two subtypes-type 1 and type 2-according to pathological findings,clinical features,and serology test results,but some cases may be defined as type not otherwise in the absence of pathological findings and inflammatory bowel *** address the differences in diagnostic criteria by country,standard diagnostic criteria for AIP were proposed in 2011 by an international consensus of expert *** diagnosis of AIP from pancreatic ductal adenocarcinoma is important but remains challenging for ***,all subtypes of AIP show dramatic response to steroid *** review discusses the current perspectives on the diagnosis and management of AIP in clinical practice.

关键词： Autoimmune pancreatitis Pancreatic cancer International consensus diagnostic criteria Steroid