检索结果-南通市图书馆

Asian Journal of Andrology 2009年第2期11卷 176-182页

作者： Fu-Jun Zhao Bang-min Han Sheng-Qiang yu Shu-Jie Xia Department of Urology Shanghai First People’s HospitalShanghai Jiao Tong UniversityShanghai 200080China

This study was designed to investigate the different involvements of prostatic stromal cells from the normal transitional zone(TZ)or peripheral zone(PZ)in the carcinogenesis of prostate cancer(PCa)epithelial cells(PC-3)in vitro and in vivo co-culture ***-structures and gene expression profiles of primary cultures of human prostatic stromal cells from the normal TZ or PZ were analyzed by electron microscopy and microarray *** vitro and in vivo co-culture models composed of normal TZ or PZ stromal cells and human PCa PC-3 cells were *** assessed tumor growth and weight in the in vivo nude mice *** are morphological and ultra-structural differences in stromal cells from TZ and PZ of the normal *** all,514 differentially expressed genes were selected by microarray analysis;483 genes were more highly expressed in stromal cells from TZ and 31 were more highly expressed in those from ***-culture with PZ stromal cells and transforming growth factor-β1(TGF-β1)increased the tumor growth of PC-3 cells in vitro and in vivo,as well as Bcl-2 *** the other hand,stromal cells of TZ suppressed PC-3 cell tumor growth in the mouse *** conclude that ultra-structures and gene expression differ between the stromal cells from TZ or PZ of the normal prostate,and stroma-epithelium interactions from TZ or PZ might be responsible for the distinct zonal localization of prostate tumor formation.

关键词： co-culture epithelial cells stromal cells transitional zone peripheral zone prostate cancer

Hepatitis B surface antigen levels during natural history of chronic hepatitis B: A Chinese perspective study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第27期20卷 9178-9184页

作者： Lin-Yan Zeng Jiang-Shan Lian Jian-Yang Chen Hong-yu Jia Yi-min Zhang Dai-Rong Xiang Liang yu Jian-Hua Hu Ying-Feng Lu Lin Zheng Lan-Juan Li Yi-Da Yang State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital of Zhejiang University School of Medicine

AIM: To determine the baseline hepatitis B surface antigen (HBsAg) levels during the different phases of chronic hepatitis B (CHB) patients in China.

关键词： Hepatitis B surface antigen quantification Chronic hepatitis B Natural history Perspective

NUV Star Catalog from the Lunar-based Ultraviolet Telescope Survey: First Release

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2016年第11期16卷 29-36页

作者： Xian-min Meng Xu-Hui Han Jian-Yan Wei Jing Wang Li Cao yu-Lei Qiu Chao Wu Jin-Song Deng Hong-Bo Cai Li-Ping Xin Key Laboratory of Space Astronomy and Technology National Astronomical Observatories Chinese Academy of Sciences

We present a star catalog extracted from the Lunar-based Ultraviolet Telescope （LUT） survey program. LUT＇s observable sky area is a circular belt around the Moon＇s north pole, and the survey program covers a preferred area of about 2400 deg2 which includes a region of the Galactic plane. The data are processed with an automatic pipeline which copes with stray light contamination, artificial sources, cosmic rays, flat field calibration, photometry and so on. In the first release version, the catalog provides high confidence sources which have been cross-identified with the Tycho-2 catalog. All the sources have signalto-noise ratio larger than 5, and the corresponding magnitude limit is typically 14.4 mag, but can be as deep as -16 mag if stray light contamination is at the lowest level. A total of 86 467 stars are recorded in the catalog. The full catalog in electronic form is available online.

关键词： Astronomical Databases: surveys -- Astronomical Databases: catalogs -- techniques: imageprocessing -- techniques: photometric -- ultraviolet: stars

涤痰汤通过调节IKB/NF-κB通路和细胞凋亡抑制阿尔茨海默病小鼠Tau蛋白过度磷酸化

在线全文

学校读者我要写书评

暂无评论

时珍国医国药 2024年第2期35卷 270-274页

作者：程美佳袁常斌鞠业涛刘勇明史宝瑞杨雅丽谢思梦杨关林闵冬雨辽宁中医药大学附属医院中医药实验中心辽宁沈阳110032 辽宁中医药大学中医脏象理论及应用教育部重点实验室辽宁沈阳110847

目的探讨涤痰汤是否可以改善阿尔茨海默病(Alzheimer's disease,AD)小鼠的认知缺陷,并明确其神经保护作用及相关机制。方法D-gal联合AlCl3造模的昆明小鼠作为AD动物模型。连续灌胃3个月后进行Y迷宫和Morris水迷宫(MWM)测试。Western blo... 详细信息

目的探讨涤痰汤是否可以改善阿尔茨海默病(Alzheimer's disease,AD)小鼠的认知缺陷,并明确其神经保护作用及相关机制。方法D-gal联合AlCl3造模的昆明小鼠作为AD动物模型。连续灌胃3个月后进行Y迷宫和Morris水迷宫(MWM)测试。Western blot检测海马组织中IKB、NF-κB、p-IKB、p-NF-κB、Bcl-2、Bax和Caspase-3蛋白的表达.同时测定海马组织Tau和p-Tau蛋白水平。结果MWM和Y迷宫结果显示,涤痰汤显著提高了AD小鼠的空间学习记忆能力。WB结果显示,涤痰汤可减少AD小鼠脑内过度磷酸化的Tau蛋白。此外,涤痰汤治疗后还抑制了AD小鼠海马p-IKB/IKB和p-NF-κB/NF-κB蛋白的表达,提示涤痰汤减少神经元凋亡可能通过IKB/NF-κB通路介导。同时,涤痰汤通过降低AD小鼠海马组织中Bax、Caspase-3蛋白表达,增加Bcl-2表达,显著抑制AD小鼠神经元凋亡。结论涤痰汤通过调节IKB/NF-κB介导的神经元细胞凋亡改善AD小鼠认知功能障碍。

关键词：阿尔兹海默病学习记忆神经元炎症凋亡涤痰汤中药复方

重组抗原捕获酶联免疫试验在云南省哨点监测人群HIV-1新发感染检测中的应用评价

在线全文

学校读者我要写书评

暂无评论

现代预防医学 2024年第10期51卷 1860-1864页

作者：梁雯丽金晓媚李东民陈会超韩孟杰邢文革张翠施玉华邱茂锋中国疾病预防控制中心性病艾滋病预防控制中心传染病溯源预警与智能决策全国重点实验室云南省疾病预防控制中心性病艾滋病防制所

目的探讨HIV-1重组抗原捕获酶联免疫试验(RAg-CEIA)在哨点监测人群HIV-1新发感染检测中的应用价值。方法针对云南省2015年艾滋病哨点监测人群，选择HIV-1抗体确证阳性血浆样本中符合新发感染检测标准的样本，使用HIV-1 RAg-CEIA进行... 详细信息

目的探讨HIV-1重组抗原捕获酶联免疫试验(RAg-CEIA)在哨点监测人群HIV-1新发感染检测中的应用价值。方法针对云南省2015年艾滋病哨点监测人群，选择HIV-1抗体确证阳性血浆样本中符合新发感染检测标准的样本，使用HIV-1 RAg-CEIA进行检测。通过标准化光密度(ODn)值识别HIV-1新近感染者，再估算人群的HIV-1新发感染率，比较HIV-1 RAg-CEIA与BED捕获酶联免疫试验(BED-CEIA)和限制性抗原亲和力酶联免疫试验(LAg-Avidity EIA)所得的检测和分析数据。结果纳入研究的哨点监测血浆样本共计51 313份，其中HIV-1抗体阳性1 255份，应进行新发感染检测373份，实检测253份。使用HIV-1 RAg-CEIA、BED-CEIA和LAg-Avidity EIA检出的HIV-1新近感染样本数分别为88、78和54份，估算哨点监测人群新发感染率分别为0.33%(95%CI:0.26%～0.39%)、0.31%(95%CI:0.24%～0.38%)和0.31%(95%CI:0.22%～0.39%),三者之间两两比较差异无统计学意义(P=0.927)。在6类亚人群中，男男性行为者(MSM)的新发感染率可估算，分别为3.50%(95%CI:2.48%～4.52%)、3.92%(95%CI:2.76%～5.08%)和4.17%(95%CI:2.79%～5.55%),三者之间两两比较差异无统计学意义(P=0.763)。HIV-1 RAg-CEIA与BED-CEIA和LAg-Avidity EIA的样本检测ODn值均呈线性相关(Spearman相关系数分别为0.911、0.755)。结论 HIV-1 RAg-CEIA可以有效用于新近感染识别和新发感染率估算，提示该方法在HIV-1新发感染检测中具有良好的应用前景。

关键词：人免疫缺陷病毒新近感染捕获酶联免疫试验哨点监测

Truncating PICK1 Variant Identified in Azoospermia Affected Mitochondrial Dysfunction in Knockout Mice

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2023年第2期43卷 313-323页

作者： Yao-qiang DU Chong-yi SHU min ZHENG Wei-de XU yue SUN Lu SHEN Chen ZHANG yu-xin ZHANG Qian-ni WANG Kai-qiang LI Bing-yu CHEN Ke HAO Jian-xin Lyu Zhen WANG Laboratory Medicine Center Department of Transfusion MedicineZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhou 310014China School of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhou 325035China Center for Reproductive Medicine Department of Reproductive EndocrinologyZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhou 310014China Department of Blood Transfusion No.903 Hospital of PLA Joint Logistic Support ForceHangzhou 310000China School of Laboratory Medicine Hangzhou Medical CollegeHangzhou 310059China

Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male *** An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the *** sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(***122SerfsX8),and this protein structure truncating variant seriously affected the biological *** we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath *** the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type ***,the mitochondrial dysfunction was verified in the *** defects in the male PICK1 knockout mice may have eventually led to complete *** The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans.

关键词： PICK1 azoospermia truncating variant knockout mice mitochondrial dysfunction

甜玉米芯多糖纳米银的合成、表征及体外活性研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

食品工业科技 2024年第13期45卷 58-66页

作者：王鑫周卓遇世友岳振歌修伟业马永强哈尔滨商业大学食品工程学院黑龙江哈尔滨150028

纳米银(AgNPs)在生物医药领域有广泛应用,但传统物理化学方法合成纳米银存在污染及产物毒性、耗时长等问题,因此探究纳米银的制备极其重要。以甜玉米芯多糖(SCP)为原料,采用绿色方法成功合成了甜玉米芯多糖纳米银(SCP-AgNPs)并对反应条... 详细信息

纳米银(AgNPs)在生物医药领域有广泛应用,但传统物理化学方法合成纳米银存在污染及产物毒性、耗时长等问题,因此探究纳米银的制备极其重要。以甜玉米芯多糖(SCP)为原料,采用绿色方法成功合成了甜玉米芯多糖纳米银(SCP-AgNPs)并对反应条件进行优化,使用透射电子显微镜(TEM)、X射线衍射仪、X光电子能谱仪、傅里叶变换红外光谱对生成的纳米银进行表征,并对其体外抗氧化及抗糖尿病能力进行探究。结果表明:SCP-AgNPs的最佳反应条件为:溶液中硝酸银浓度为0.75×10^(-3)mol/L,pH为10.0,反应80 min,所制备的SCP-AgNPs呈球状,平均粒径为13.5 nm。结构表现为面心立方晶体结构,确定其中Ag为Ag0状态。合成的SCP-AgNPs有较强的体外清除自由基作用。同时,SCP-AgNPs对α-葡萄糖苷酶和α-淀粉酶这两种糖尿病标记酶的活性具有良好的抑制作用。SCP-AgNPs对糖尿病关键酶具有显著的抑制效果,具备潜在抗糖尿病活性,是一种适合纳米生物医学应用的纳米药物,未来将广泛应用于食品加工、医学等领域。

关键词：甜玉米芯多糖银纳米颗粒表征抗氧化抗糖尿病

维普期刊数据库博看期刊

Calibration of the DAMPE Plastic Scintillator Detector and its on-orbit performance

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2019年第3期19卷 145-154页

作者： Meng Ding Ya-Peng Zhang Yong-Jie Zhang yuan-Peng Wang Tie-Kuang Dong Antonio De Benedittis Paolo Bernardini Fang Fang Yao Li Jie Liu Peng-Xiong Ma Zhi-yu Sun Valentina Gallo Stefania Vitillo Zhao-min Wang yu-Hong yu Chuan yue Qiang yuan Yong Zhou yun-Long Zhang Institute of Modern Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Dark Matter and Space Astronomy Purple Mountain Observatory Chinese Academy of Sciences School of Astronomy and Space Science University of Science and Technology of China Istituto Nazionale di Fisica Nucleare (INFN) - Sezione di Lecce Dipartimento di Matematica e Fisica E.De Giorgi Universit`a del Salento Department of Nuclear and Particle Physics University of Geneva Gran Sasso Science Institute INFN Laboratori Nazionali del Gran Sasso Department of Modern Physics University of Science and Technology of China

The DArk Matter Particle Explorer(DAMPE) is a space-borne apparatus for detecting the highenergy cosmic-ray-like electrons, γ-rays, protons and heavy ions. The Plastic Scintillator Detector(PSD)is the top-most sub-detector of the DAMPE. The PSD is designed to measure the charge of incident highenergy particles and it also serves as a veto detector for discriminating γ-rays from charged particles. In this paper, a PSD on-orbit calibration procedure is described, which includes the five steps of pedestal, dynode correlation, response to minimum-ionizing particles, light attenuation function and energy reconstruction.A method for reconstructing the charge of incident high energy cosmic-ray particles is introduced. The detection efficiency of each PSD strip is verified to be above 99.5%; the total efficiency of the PSD for charged particles is above 99.99%.

关键词： cosmic ray instrumentation: DAMPE charge measurement plastic scintillator detector calibration

免疫磁珠富集水中鲤春病毒血症病毒的初步研究

在线全文

学校读者我要写书评

暂无评论

中国预防兽医学报 2024年第6期46卷 641-644页

作者：景宏丽孔玉方斯烈钢王建平葛明峰徐胜威黄呈炜高隆英张旻吴绍强中国检验检疫科学研究院北京100176 宁波市海洋与渔业研究院浙江宁波315012 中华人民共和国大铲湾海关广东深圳518100

为建立一种富集水中鲤春病毒血症病毒(SVCV)的技术,本研究利用SVCV多克隆抗体制备免疫磁珠,通过对磁珠耦联抗体量的优化,确定该免疫磁珠有效富集SVCV的最低浓度,建立一种富集SVCV的免疫磁珠分离技术,并联合荧光定量RT-PCR(RT-qPCR)技术... 详细信息

为建立一种富集水中鲤春病毒血症病毒(SVCV)的技术,本研究利用SVCV多克隆抗体制备免疫磁珠,通过对磁珠耦联抗体量的优化,确定该免疫磁珠有效富集SVCV的最低浓度,建立一种富集SVCV的免疫磁珠分离技术,并联合荧光定量RT-PCR(RT-qPCR)技术对水中SVCV进行检测。结果显示,1 mg磁珠与75μg SVCV多克隆抗体耦联时,耦联的抗体量最大,耦联率为98.48%;耦联后的免疫磁珠对SVCV(200μL,浓度为1.54×10^(6)拷贝/μL)的富集效率最高为91.56%。以该多克隆抗体量制备的免疫磁珠富集10倍倍比稀释的SVCV(3.54×10^(6)拷贝/μL~3.54×10^(3)拷贝/μL),以确定其有效富集SVCV的最低浓度。结果显示,1 mg免疫磁珠能够使200μL病毒液(3.54×10^(3)拷贝/μL)富集率达到61.58%。表明该免疫磁珠能够有效富集SVCV。在免疫磁珠分离技术联合RT-qPCR技术检测水样品中SVCV(浓度约为1.16×10^(4)拷贝/μL)的结果显示,免疫磁珠组富集SVCV核酸量是裸磁珠组富集SVCV核酸量的1.77×10^(3)倍,是未处理组富集SVCV核酸量的1.68×10^(3)倍,免疫磁珠组富集SVCV均极显著高于裸磁珠组和未处理组(P0.05)。本研究首次建立了一种能够有效富集水中SVCV的方法,为SVCV的监测提供了技术手段。

关键词：免疫磁珠鲤春病毒血症病毒水样品

环泊酚联合阿芬太尼用于支气管镜检查术患者的临床研究

在线全文

学校读者我要写书评

暂无评论

中国临床药理学杂志 2024年第19期40卷 2806-2810页

作者：回宇峰谭思琪孙莹杰中国人民解放军北部战区总医院麻醉科辽宁沈阳110016

目的比较环泊酚注射液与丙泊酚注射液分别联合阿芬太尼注射液用于喉罩通气全身麻醉下行气管镜检查术患者的临床疗效及安全性。方法将拟行气管镜检查患者随机分为试验组和对照组。试验组静脉推注20μg·kg^(-1)阿芬太尼、0.4 mg·kg^(-1... 详细信息

目的比较环泊酚注射液与丙泊酚注射液分别联合阿芬太尼注射液用于喉罩通气全身麻醉下行气管镜检查术患者的临床疗效及安全性。方法将拟行气管镜检查患者随机分为试验组和对照组。试验组静脉推注20μg·kg^(-1)阿芬太尼、0.4 mg·kg^(-1)环泊酚和0.2 mg·kg^(-1)顺式阿曲库铵进行麻醉诱导;对照组静脉推注20μg·kg^(-1)阿芬太尼、2 mg·kg^(-1)丙泊酚和0.2 mg·kg^(-1)顺势式阿曲库铵进行麻醉诱导。2组患者均用喉罩通气全身麻醉。试验组静脉给予0.8 mg·kg^(-1)·h^(-1)环泊酚和0.5~1.0μg·kg^(-1)·min^(-1)阿芬太尼进行麻醉维持;对照组静脉给予8 mg·kg^(-1)·h^(-1)丙泊酚和0.5~1.0μg·kg^(-1)·min^(-1)阿芬太尼进行麻醉维持。比较2组患者麻醉过程中的生命体征、麻醉诱导和恢复时间、阿芬太尼用量,以及安全性。结果试验组入组70例,脱落10例,最终有60例纳入统计分析;对照组入组70例,脱落10例,最终有60例纳入统计分析。在诱导3 min后(T1),试验组和对照组的平均动脉压(MAP)分别为(79.32±5.73)和(73.15±6.20)mmHg,心率(HR)分别为(70.53±8.20)和(65.77±7.75)beat·min^(-1);在置入支气管镜时(T2),试验组和对照组的MAP分别为(82.52±5.81)和(75.99±6.09)mmHg,HR分别为(70.27±7.94)和(65.42±7.73)beat·min^(-1);试验组在T1、T2时的MAP和HR均显著高于同时刻的对照组,在统计学上差异均有统计学意义(均Pmin,术中阿芬太尼用量分别为(3456.67±608.51)和(3515.00±619.41)μg,在统计学上差异均无统计学意义(均P>0.05)。试验组和对照组在诱导期注射痛发生率分别为3.33%和30.00%,术中低血压发生率分别为18.33%和40.00%,术中心动过缓发生率分别为3.33%和13.33%,在统计学上差异均有统计学意义(均P<0.05)。结论相比于丙泊酚注射液联合阿芬太尼注射液,环泊酚注射液联合阿芬太尼注射液用于喉罩通气全身麻醉下行气管镜检查术患者在麻醉诱导和维持期能够更好地维持血流动力学稳定,且安全性较好。

关键词：环泊酚注射液丙泊酚注射液阿芬太尼注射液支气管镜检查安全性评价