Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate

作     者：Fu-Jun Zhao Bang-Min Han Sheng-Qiang Yu Shu-Jie Xia 

作者机构：Department of UrologyShanghai First People’s HospitalShanghai Jiao Tong UniversityShanghai 200080China 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2009年第11卷第2期

页      面：176-182页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.30672109) 

主　　题：co-culture epithelial cells stromal cells transitional zone peripheral zone prostate cancer 

摘      要：This study was designed to investigate the different involvements of prostatic stromal cells from the normal transitional zone(TZ)or peripheral zone(PZ)in the carcinogenesis of prostate cancer(PCa)epithelial cells(PC-3)in vitro and in vivo co-culture ***-structures and gene expression profiles of primary cultures of human prostatic stromal cells from the normal TZ or PZ were analyzed by electron microscopy and microarray *** vitro and in vivo co-culture models composed of normal TZ or PZ stromal cells and human PCa PC-3 cells were *** assessed tumor growth and weight in the in vivo nude mice *** are morphological and ultra-structural differences in stromal cells from TZ and PZ of the normal *** all,514 differentially expressed genes were selected by microarray analysis;483 genes were more highly expressed in stromal cells from TZ and 31 were more highly expressed in those from ***-culture with PZ stromal cells and transforming growth factor-β1(TGF-β1)increased the tumor growth of PC-3 cells in vitro and in vivo,as well as Bcl-2 *** the other hand,stromal cells of TZ suppressed PC-3 cell tumor growth in the mouse *** conclude that ultra-structures and gene expression differ between the stromal cells from TZ or PZ of the normal prostate,and stroma-epithelium interactions from TZ or PZ might be responsible for the distinct zonal localization of prostate tumor formation.