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The spliceophilin CYP18-2 is mainly involved in the splicing of retained introns under heat stress in Arabidopsis

Journal of Integrative Plant Biology 2023年第5期65卷 1113-1133页

作者： Areum Lee hyun Ji Park Seung Hee Jo Haemyeong Jung hyun-Soon kim Hyo-Jun Lee Youn-Sung kim Choonkyun Jung Hye Sun Cho Plant Systems Engineering Research Center Korea Research Institute of Bioscience and Biotechnology(KRIBB)Daejeon 34141Korea Department of Biosystems and Bioengineering KRIBB School of BiotechnologyUniversity of Science and Technology(UST)Daejeon 34141Korea Department of Functional Genomics KRIBB School of BioscienceUSTDaejeon 34113Korea Department of Biotechnology NongWoo BioAnseong 17558Korea Department of International Agricultural Technology and Crop Biotechnology Institute/Green Bio Science and Technology Seoul National UniversityPyeongchang 25354Korea Department of Agriculture Forestryand Bioresources and Integrated Major in Global Smart FarmCollege of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Korea

Peptidyl-prolyl isomerase-like 1(PPIL1)is associated with the human spliceosome ***,its function in pre-mRNA splicing remains *** this study,we show that Arabidopsis thaliana CYCLOPHILIN 18-2(AtCYP18-2),a PPIL1 homolog,plays an essential role in heat tolerance by regulating pre-mRNA *** heat stress conditions,AtCYP18-2 expression was upregulated in mature plants and GFP-tagged AtCYP18-2 redistributed to nuclear and cytoplasmic *** determined that AtCYP18-2 interacts with several spliceosome complex B^(ACT)components in nuclear puncta and is primarily associated with the small nuclear RNAs U5 and U6 in response to heat *** AtCYP18-2 loss-of-function allele cyp18-2 engineered by CRISPR/Cas9-mediated gene editing exhibited a hypersensitive phenotype to heat stress relative to the wild ***,global transcriptome profiling showed that the cyp18-2 mutation affects alternative splicing of heat stress–responsive genes under heat stress conditions,particularly intron retention(IR).The abundance of most intron-containing transcripts of a subset of genes essential for thermotolerance decreased in cyp18-2 compared to the wild ***,the intron-containing transcripts of two heat stress-related genes,HEAT SHOCK PROTEIN 101(HSP101)and HEAT SHOCK FACTOR A2(HSFA2),produced functional ***101-IR-GFP localization was responsive to heat stress,and HSFA2-Ⅲ-IR interacted with HSF1 and HSP90.1 in plant *** findings reveal that CYP18-2 functions as a splicing factor within the B~(ACT)spliceosome complex and is crucial for ensuring the production of adequate levels of alternatively spliced transcripts to enhance thermotolerance.

关键词： alternative splicing CYP18-2 HSFA2-Ⅲ intron retention spliceophilin spliceosome complex B~(ACT) thermotolerance

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Distribution of electric field for carbon nanotube assembly: Experiments (II)

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Transactions of Nonferrous Metals Society of China 2011年第A1期21卷 121-125页

作者： Soongeun KWON Soo-hyun kim kwang-ho kim Myung-chang KANG hyung-woo LEE Department of Mechanical Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon 305-701 Korea National Core Research Center for Hybrid Materials Solution Pusan National University

The distribution effect of electric field on the alignment and attachment of carbon nanotubes (CNTs) were investigated. The experimental results were compared with the simulation results according to three different shaped electrodes. In previous simulation, the round shaped electrodes were expected to be more effective for aligning and attaching a single CNT between two electrodes than conical or rectangular shaped electrodes. To verify the simulation results, three different shaped electrodes were introduced and a single multi-walled carbon nanotube (MWNT) was attached. The optimal conditions for aligning and attaching MWNTs such as the frequency, applied voltage and concentration of MWNTs solution were investigated. Through repeated experiments, frequency of 100 kHz-10 MHz, applied voltage of 0.3-1.3 Vrms/μm, concentration of 5 μg/mL in MWNTs solution were obtained as a possible condition range to attach MWNTs. Under these conditions, the yield of MWNTs attachment between two electrodes was up to 70%. In previous simulation, furthermore, it was verified that the size of the stable or quasi-stable region made CNTs aligned and attached on different shaped electrodes from the comparison of the experimental and simulation results. Most single MWNT attachment was accomplished on the round shaped electrodes.

关键词：多壁碳纳米管电场分布实验组装单壁碳纳米管稳定区域 MWNT 最佳条件

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Accelerated biodegradation of iron-based implants via tantalum-implanted surface nanostructures

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Bioactive Materials 2022年第3期7卷 239-250页

作者： Min-Kyu Lee hyun Lee Cheonil Park In-Gu Kang Jinyoung kim Hyoun-Ee kim hyun-Do Jung Tae-Sik Jang Department of Materials Science and Engineering Seoul National UniversitySeoul08826Republic of Korea Querrey Simpson Institute for Bioelectronics Northwestern UniversityEvanstonIL60208USA Department of Biomedical-Chemical Engineering Catholic University of KoreaBucheon14662Republic of Korea Department of Biotechnology The Catholic University of KoreaBucheon14662Republic of Korea Department of Materials Science and Engineering Chosun UniversityGwangju61452Republic of Korea

In recent years,pure iron(Fe)has attracted significant attention as a promising biodegradable orthopedic implant material due to its excellent mechanical and biological ***,in physiological conditions,Fe has an extremely slow degradation rate with localized and irregular degradation,which is problematic for practical *** this study,we developed a novel combination of a nanostructured surface topography and galvanic reaction to achieve uniform and accelerated degradation of an Fe *** target-ion induced plasma sputtering(TIPS)technique was applied on the Fe implant to introduce biologically compatible and electrochemically noble tantalum(Ta)onto its surface and develop surface nano-galvanic *** tests revealed that the uniformly distributed nano-galvanic corrosion cells of the TIPS-treated sample(nano Ta-Fe)led to relatively uniform and accelerated surface degradation compared to that of bare ***,the mechanical properties of nano Ta-Fe remained almost constant during a long-term in vitro immersion test(~40 weeks).Biocompatibility was also assessed on surfaces of bare Fe and nano Ta-Fe using in vitro osteoblast responses through direct and indirect contact assays and an in vivo rabbit femur medullary cavity implantation *** results revealed that nano Ta-Fe not only enhanced cell adhesion and spreading on its surface,but also exhibited no signs of cellular or tissue *** results demonstrate the immense potential of Ta-implanted surface nanostructures as an effective solution for the practical application of Fe-based orthopedic implants,ensuring long-term biosafety and clinical efficacy.

关键词： Iron Tantalum Ion implantation Biodegradation Orthopedic implants

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Unveiling the redox electrochemistry of 1D,urchin-like vanadium sulfide electrodes for high-performance hybrid supercapacitors

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Journal of Energy Chemistry 2023年第4期79卷 569-580页

作者： K.Karuppasamy Dhanasekaran Vikraman Sajjad Hussain Balamurugan Thirumalraj P.Santhoshkumar Hemalatha Parangusan hyun-Chang Park Jongwan Jung hyun-Seok kim Division of Electronics and Electrical Engineering Dongguk University-SeoulSeoul 04620Republic of Korea Department of Nanotechnology and Advanced Materials Engineering Sejong UniversitySeoul 05006Republic of Korea School of Materials Science&Engineering Kookmin UniversitySeoul 02707Republic of Korea Millimeter-Wave Innovation Technology Research Center(MINT) Dongguk University-SeoulSeoul 04620Republic of Korea Qatar University Young Scientist Center(QUYSC) Qatar UniversityP.O.Box 2713DohaQatar

Exploring novel versatile electrode materials with outstanding electrochemical performance is the key to the development of advanced energy conversion and storage *** this work,we aim to construct new-fangled one-dimensional(1D)quasi-layered patronite vanadium tetrasulfide(VS_(4))nanostructures by using different sulfur sources,namely thiourea,thioacetamide,and L-cysteine through an ethyleneaminetetraacetic-acid(EDTA)-mediated solvothermal *** as-prepared VS4exhibits several unique morphologies such as urchin,fluffy nanoflower,and polyhedron with appropriate surface *** the prepared nanostructures,the VS_(4)-1@NF nanostructure exhibited excellent electrochemical properties in 6 M KOH solution,and we explored its redox electrochemistry in *** asprepared VS_(4)-1@NF electrode exhibited battery-type redox characteristics with the highest capacity of280 C g^(-1)in a three-electrode ***,it offered a capacity of 123 F g^(-1)in a hybrid twoelectrode set-up at 1 A g^(-1)with the highest specific energy and specific power of 38.5 W h kg^(-1)and750 W kg^(-1),***,to ensure the practical applicability and real-world performance of the prepared hybrid AC@NF//VS_(4)-1@NF cell,we performed a cycling stability test with more than 5,000galvanostatic charge–discharge cycles at 2 A g^(-1),and the cell retained around 84.7%of its capacitance even after 5,000 cycles with a CE of 96.1%.

关键词： Patronite Redox electrochemistry Urchin VS_(4) 1D material Mesoporous

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Simultaneous robot-assisted approach in a super-elderly patient with urothelial carcinoma and synchronous contralateral renal cell carcinoma:A case report

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World Journal of Clinical Cases 2022年第20期10卷 7153-7162页

作者： Ji Kang Yun Si hyun kim Woong Bin kim Hee Kyung kim Sang Wook Lee Department of Urology Soonchunhyang University Bucheon HospitalSoonchunhyang University College of MedicineBucheon 14584South Korea Department of Urology Soonchunhyang University Cheonan HospitalSoonchunhyang University College of MedicineCheonan 31151South Korea Department of Pathology Soonchunhyang University Bucheon HospitalSoonchunhyang University College of MedicineBucheon 14584South Korea

BACKGROUND It is rare for urothelial and renal cell carcinomas to coexist in the same patient,and even rarer for them to be detected *** of this rarity,a standard treatment has not been established and studies about overall survival are ***,physicians must modify treatments according to the individual’s situation and the stage of each *** recent years,with advances in the instruments and techniques,minimal invasive robotic surgeries have become available for advanced-stage or high-risk *** SUMMARY An 85-year-old woman with a medical history of hypertension and hyperlipidemia visited our *** had visited her local hospital complaining of intermittent,painless,gross hematuria that had started 3 mo *** computed tomography,a right renal mass and left proximal ureteral mass with hydronephrosis were found *** decided to perform robot-assisted surgery on both sides during one *** renal function and kidney loading,right partial nephrectomy was performed first,followed by left nephroureterectomy with bladder cuff *** the 6-mo follow-up,no specific symptoms were reported and dialysis was not *** were no unusual findings in the imaging study,and regular follow-up and imaging studies are *** This case report assessed the feasibility of simultaneous minimal invasive robotic surgery as an alternative to conventional open or laparoscopic surgery.

关键词： Robot surgical procedure Renal cell carcinoma Transitional cell carcinoma Minimally invasive surgical procedures Case report

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Two-Terminal Lithium-Mediated Artificial Synapses with Enhanced Weight Modulation for Feasible Hardware Neural Networks

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Nano-Micro Letters 2023年第5期15卷 236-253页

作者： Ji hyun Baek Kyung Ju Kwak Seung Ju kim Jaehyun kim Jae Young kim In Hyuk Im Sunyoung Lee Kisuk Kang Ho Won Jang Department of Materials Science and Engineering Research Institute of Advanced MaterialsSeoul National UniversitySeoul 08826Republic of Korea Advanced Institute of Convergence Technology Seoul National UniversitySuwon 16229Korea

Recently,artificial synapses involving an electrochemical reaction of Li-ion have been attributed to have remarkable synaptic ***-terminal synaptic transistors utilizing Li-ion intercalation exhibits reliable synaptic characteristics by exploiting the advantage of nondistributed weight updates owing to stable ion ***,the three-terminal configurations with large and complex structures impede the crossbar array implementation required for hardware neuromorphic ***,achieving adequate synaptic performances through effective Li-ion intercalation in vertical two-terminal synaptic devices for array integration remains ***,two-terminal Au/LixCoO_(2)/Pt artificial synapses are proposed with the potential for practical implementation of hardware neural *** Au/LixCoO_(2)/Pt devices demonstrated extraordinary neuromorphic behaviors based on a progressive dearth of Li in LixCoO_(2)*** intercalation and deintercalation of Li-ion inside the films are precisely controlled over the weight control spike,resulting in improved weight control *** types of synaptic plasticity were imitated and assessed in terms of key factors such as nonlinearity,symmetricity,and dynamic ***,the LixCoO_(2)-based neuromorphic system outperformed three-terminal synaptic transistors in simulations of convolutional neural networks and multilayer perceptrons due to the high linearity and low programming *** impressive performances suggest the vertical two-terminal Au/LixCoO_(2)/Pt artificial synapses as promising candidates for hardware neural networks.

关键词： Artificial synapse Neuromorphic Li-based Two-terminal Synaptic plasticity

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Rapid hemostasis of the residual inguinal access sites during endovascular procedures:A case report

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World Journal of Clinical Cases 2022年第34期10卷 12684-12689页

作者： Hyangkyoung kim kwangjin Lee Sungsin Cho Jin hyun Joh Department of Vascular Surgery Kyung Hee University Hospital at GangdongSeoul 05278South Korea Department of Surgery Kyung Hee University Hospital at GangdongSeoul 05278South Korea

BACKGROUND In endovascular procedures including total percutaneous endovascular aneurysm repair(pEVAR),percutaneous access through the common femoral artery is most commonly ***-site bleeding is a major concern in percutaneous ***,we present a case of successful control of continuous oozing using a vascular closure device(VCD)and the application of Surgicel(Johnson&Johnson,United States)over the access *** SUMMARY An 82-year-old man presented with an unruptured abdominal aortic aneurysm measuring 83 mm×75 *** patient had a medical history of atrial fibrillation and was receiving rivaroxaban(15 mg/d).Routine pEVAR was performed using the preclose technique with ProGlide(Abbott,Santa Clara,CA,United States).Significant amount of bleeding was observed at the end of the procedure after the deployment of the closure device at the access site.A sheet of Surgicel was applied to the suture thread using a surgical *** was applied to the surface of the artery along the access tract using a pusher,and hemostasis was immediately *** This simple technique is an excellent adjunct to control residual bleeding from the access site following VCD use.

关键词： Endovascular procedures Punctures Hemostasis Hemostatic techniques Femoral artery Case report

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ESG Discourse Analysis Through BERTopic: Comparing News Articles and Academic Papers

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Computers, Materials & Continua 2023年第6期75卷 6023-6037页

作者： Haein Lee Seon Hong Lee Kyeo Re Lee Jang hyun kim Department of Applied Artificial Intelligence/Department of Human-Artificial Intelligence Interaction Sungkyunkwan UniversitySeoul03063Korea Center for SW Education Hanyang UniversityAnsan15588Korea Department of Interaction Science/Department of Human-Artificial Intelligence Interaction Sungkyunkwan UniversitySeoul03063Korea

Environmental,social,and governance(ESG)factors are critical in achieving sustainability in business management and are used as values aiming to enhance corporate ***,non-financial indicators have been considered as important for the actual valuation of corporations,thus analyzing natural language data related to ESG is *** previous studies limited their focus to specific countries or have not used big *** methodologies are insufficient for obtaining potential insights into the best practices to leverage *** address this problem,in this study,the authors used data from two platforms:LexisNexis,a platform that provides media monitoring,and Web of Science,a platform that provides scientific *** big data were analyzed by topic *** modeling can derive hidden semantic structures within the *** this process,it is possible to collect information on public and academic *** authors explored data from a text-mining perspective using bidirectional encoder representations from transformers topic(BERTopic)—a state-of-the-art topic-modeling *** addition,changes in subject patterns over time were considered using dynamic topic *** a result,concepts proposed in an international organization such as the United Nations(UN)have been discussed in academia,and the media have formed a variety of agendas.

关键词： ESG BERTopic natural language processing topic modeling

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Obesity and novel management of inflammatory bowel disease

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World Journal of Gastroenterology 2023年第12期29卷 1779-1794页

作者： Jee hyun kim Chang-Myung Oh Jun Hwan Yoo Department of Gastroenterology CHA Bundang Medical CenterCHA University School of MedicineSeongnam 13496South Korea Department of Biomedical Science and Engineering Gwangju Institute of Science and TechnologyGwangju 62465South Korea Institute of Basic Medical Sciences CHA University School of MedicineSeongnam 13496South Korea

Obesity is prevalent within the inflammatory bowel disease(IBD)population,particularly in newly developed *** epidemiological studies have suggested that 15%-40%of IBD patients are obese,and there is a potential role of obesity in the pathogenesis of *** dysfunction of mesenteric fat worsens the inflammatory course of Crohn’s disease and may induce formation of strictures or ***,obesity may affect the disease course or treatment response of *** the increasing data supporting the pathophysiologic and epidemiologic relationship between obesity and IBD,obesity control is being suggested as a novel management for ***,this review aimed to describe the influence of obesity on the outcomes of IBD treatment and to present the current status of pharmacologic or surgical anti-obesity treatments in IBD patients.

关键词： Obesity Inflammatory bowel disease Crohn’s disease Ulcerative colitis

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Memristive Devices Based on Two-Dimensional Transition Metal Chalcogenides for Neuromorphic Computing

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Nano-Micro Letters 2022年第4期14卷 29-58页

作者： Ki Chang Kwon Ji hyun Baek Kootak Hong Soo Young kim Ho Won Jang Department of Materials Science and Engineering Research Institute of Advanced MaterialsSeoul National UniversitySeoul 08826Republic of Korea Interdisciplinary Materials Measurement Institute Korea Research Institute of Standards and Science(KRISS)Daejeon 34133Republic of Korea Department of Materials Science and Engineering Institute of Green Manufacturing TechnologyKorea UniversitySeoul 02841Republic of Korea Advanced Institute of Convergence Technology Seoul National UniversitySuwon 16229Korea

Two-dimensional(2D)transition metal chalcogenides(TMC)and their heterostructures are appealing as building blocks in a wide range of electronic and optoelectronic devices,particularly futuristic memristive and synaptic devices for brain-inspired neuromorphic computing *** distinct properties such as high durability,electrical and optical tunability,clean surface,flexibility,and LEGO-staking capability enable simple fabrication with high integration density,energy-efficient operation,and high *** review provides a thorough examination of high-performance memristors based on 2D TMCs for neuromorphic computing applications,including the promise of 2D TMC materials and heterostructures,as well as the state-of-the-art demonstration of memristive *** challenges and future prospects for the development of these emerging materials and devices are also *** purpose of this review is to provide an outlook on the fabrication and characterization of neuromorphic memristors based on 2D TMCs.

关键词： Two-dimensional materials Memristors Neuromorphic computing Artificial synapses Transition metal chalcogenides

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