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Family Competition Pheromone Genetic Algorithm for Comparative Genome Assembly

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Journal of Electronic Science and Technology 2014年第4期12卷 405-409页

作者： Chien-Hao Su Chien-Shun Chiou Jung-Che kuo Pei-Jen Wang Cheng-Yan Kao Hsueh-Ting chu the Biodiversity Research Center Academia Sinica the Center for Research Diagnostics and Vaccine Development Centers for Disease Control the Department of Computer Science and Information Engineering National Taiwan University the Department of Computer Science and Information Engineering Asia University

Genome assembly is a prerequisite step for analyzing next generation sequencing data and also far from being solved. Many assembly tools have been proposed and used extensively. Majority of them aim to assemble sequencing reads into contigs; however, we focus on the assembly of contigs into scaffolds in this paper. This is called scaffolding, which estimates the relative order of the contigs as well as the size of the gaps between these contigs. Pheromone trail-based genetic algorithm （PGA） was previously proposed and had decent performance according to their paper. From our previous study, we found that family competition mechanism in genetic algorithm is able to further improve the results. Therefore, we propose family competition pheromone genetic algorithm （FCPGA） and demonstrate the improvement over PGA.

关键词： Genetic algorithm genome typing,next generation sequencing.

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预氧化时间对中空多孔炭纤维介电常数的影响

新型炭材料 2011年第6期26卷 441-445页

作者：谢炜陈朝辉程海峰楚增勇匡加才长沙理工大学汽车与机械工程学院湖南长沙410114 国防科技大学新型陶瓷纤维及其复合材料国防科技重点实验室湖南长沙410073 长沙理工大学材料科学与工程研究所湖南长沙410114

以中空多孔聚丙烯腈(polyacrylonitrile,PAN)原丝为原料,通过预氧化和炭化工艺制备了中空多孔炭纤维(Hollow-porous carbon fibres,HPCFs)。研究了预氧化时间对其微观结构、元素组成、电导率和介电常数的影响。结果表明:预氧化时间对所... 详细信息

以中空多孔聚丙烯腈(polyacrylonitrile,PAN)原丝为原料,通过预氧化和炭化工艺制备了中空多孔炭纤维(Hollow-porous carbon fibres,HPCFs)。研究了预氧化时间对其微观结构、元素组成、电导率和介电常数的影响。结果表明:预氧化时间对所获HPCFs微观结构的影响不大;HPCFs的碳含量随预氧化时间增加而减少,而氮含量则随预氧化时间增加而增加。随着预氧化时间增加,所得HPCFs电导率和介电常数依次降低。预氧化时间60 min、炭化温度800℃条件下所制HPCFs在2 GHz处的介电常数实部和虚部分别为17.20和11.90,对应的电导率为564.50Ω-.1m-1。可通过热处理工艺调节HPCFs的电导率和介电常数。

关键词：中空多孔炭纤维氧化炭化介电常数

Predictors of the outcomes of acute-on-chronic hepatitis B liver failure

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World Journal of Gastroenterology 2012年第36期18卷 5078-5083页

作者： Hsiu-Lung Fan Po-Sheng Yang Hui-Wei Chen Teng-Wei Chen De-chuan Chan Chi-Hong chu Jyh-Cherng Yu Shih-Ming kuo chung-Bao Hsieh Division of General Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical Center Division of General Surgery Department of SurgeryMackay Memorial Hospital School of Medicine University of PittsburghPittsburghPA 15261United States Division of Pediatric Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical Center

AIM： To identify the risk factors in predicting the out- come of acute-on-chronic hepatitis B liver failure pa- tients. METHODS： We retrospectively divided 113 patients with acute-on-chronic liver failure-hepatitis B virus （ACLF-HBV） and without concurrent hepatitis C or D virus infection and hepatocellular carcinoma into two groups according to their outcomes after anti-HBV therapy. Their demographic, clinical, and biochemical data on the day of diagnosis and after the first week of treatment were analyzed using the Mann-Whitney U test, Fisher＇s exact test, and a multiple logistic regres- sion analysis. RESULTS： The study included 113 patients （87 men and 26 women） with a mean age of 49.84 years. Fifty- two patients survived, and 61 patients died. Liver failure （85.2%）, sepsis （34.4%）, and multiple organ failure （39.3%） were the main causes of death. Mul- tivariate analyses showed that Acute Physiology and Chronic Health Evaluation （APACHE） Ⅱ scores ≥ 12 [odds ratio （OR） = 7.160, 95% CI： 2.834-18.092, P 〈 0.001] and positive blood culture （OR = 13.520, 95% CI： 2.740-66.721, P = 0.001） on the day of diagnosis and model for end-stage liver disease （MELD） scores 28 （OR = 8.182, 95% CI： 1.884-35.527, P = 0.005） after the first week of treatment were independent predictors of mortality. CONCLUSION： APACHE II scores on the day of diag- nosis and MELD scores after the first week of anti-HBV therapy are feasible predictors of outcome in ACLF- HBV patients.

关键词： Lamivudine Liver failure Hepatitis B virus Acute Physiology and Chronic Health Evaluation ]]score Model for end-stage liver disease scores

肝水解肽的制备及其病毒灭活/去除工艺的验证

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中国生物制品学杂志 2010年第1期23卷 95-97页

作者：辜正平王伯初旷瑜重庆大学生物工程学院重庆400044

目的制备肝水解肽,并对其病毒灭活/去除工艺进行验证。方法以鸡新城疫病毒(NDV)和鸡传染性法氏囊病毒(IBDV)为指示病毒,在100℃,pH6.0～7.0煮沸20min灭活病毒,用截留相对分子质量为10000的中空纤维柱,在进压0.15Mpa、回流压0.05Mpa和进... 详细信息

目的制备肝水解肽,并对其病毒灭活/去除工艺进行验证。方法以鸡新城疫病毒(NDV)和鸡传染性法氏囊病毒(IBDV)为指示病毒,在100℃,pH6.0～7.0煮沸20min灭活病毒,用截留相对分子质量为10000的中空纤维柱,在进压0.15Mpa、回流压0.05Mpa和进压0.20Mpa、回流压0.10Mpa各循环超滤3次去除病毒,用无特定病原(SPF)的鸡胚和鸡成纤维细胞进行病毒灭活/去除的效果验证。结果加热煮沸后,指示病毒未检出,滴度降低NDV≥7.4logEID50/0.2ml、IBDV≥5.45logCCID50/0.1ml;超滤后IBDV未检出,滴度降低≥5.43logCCID50/0.1ml。盲传复壮均未检出病毒。制备的肝水解肽多肽含量均在20mg/ml以上。结论制备的肝水解肽工艺中两种灭活/去除病毒方法均有效,经灭活/去除病毒后的产品质量稳定。

关键词：肝水解肽病毒灭活/去除工艺验证

基于CFD-DEM算法的固体火箭发动机气-固两相流模拟

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推进技术 2019年第7期40卷 1546-1553页

作者：杨文婧匡亮褚开维刘佩进西北工业大学航天学院燃烧、流动和热结构国家级重点实验室陕西西安710072 蒙纳士大学化工学院 ARE颗粒仿真技术研究中心维多利亚州3800澳大利亚

针对固体火箭发动机中含金属推进体系中颗粒冲刷烧蚀及推力两相流损失的难题,基于连续相-离散元(CFD-DEM)耦合模型,考虑颗粒间的碰撞力与非碰撞力以及颗粒-气流作用力等,对固体发动机中气-固两相流进行了数值模拟研究。研究结果表明,相... 详细信息

针对固体火箭发动机中含金属推进体系中颗粒冲刷烧蚀及推力两相流损失的难题,基于连续相-离散元(CFD-DEM)耦合模型,考虑颗粒间的碰撞力与非碰撞力以及颗粒-气流作用力等,对固体发动机中气-固两相流进行了数值模拟研究。研究结果表明,相比于传统的双流体模型及轨道法,CFD-DEM能提供更丰富的粒子尺度信息包括粒子的运动轨迹、颗粒间的碰撞、颗粒受力情况等等;以及粒子相与气相相互作用过程,粒子相与壁面相互作用等。粒子的碰撞在喷管收敛段壁面、喉部区域以及喷管扩张段中心区域发生,与壁面的碰撞在收敛段壁面位置,且颗粒在该区域角速度较大,气相-颗粒曳力最大值出现在气相加速区域。单个粒子尺度的流场数据为固体发动机气固两相流流场的认识提供了更加丰富的信息,并为粒子聚集聚合及对烧蚀层冲刷等提供研究基础。

关键词：固体火箭发动机离散元方法气固两相流数值模拟

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一期全耳廓再造耳支架覆盖组织瓣的模板设计

中华整形外科杂志 2006年第6期22卷 430-433页

作者：匡建国楚建军唐胜建山东潍坊医学院整形外科研究所潍坊261042 复旦大学附属眼耳鼻喉科医院整形外科

目的研究耳支架覆盖组织瓣的模板设计,探讨一种精确可靠的组织瓣设计方法。方法运用工程图学理论和CT三维测量技术,对40只发育正常耳廓的表面三维形态进行近似平面展开,据此展开图形设计耳支架覆盖组织瓣模板。结果健康人耳廓表面三维... 详细信息

目的研究耳支架覆盖组织瓣的模板设计,探讨一种精确可靠的组织瓣设计方法。方法运用工程图学理论和CT三维测量技术,对40只发育正常耳廓的表面三维形态进行近似平面展开,据此展开图形设计耳支架覆盖组织瓣模板。结果健康人耳廓表面三维形态的展开图形具有大致相似的基本形状,据此基本图形的关键点可绘制出SMAS组织瓣的简化模板。结论应用CT三维测量模板设计耳支架覆盖组织瓣准确可靠。

关键词：计算机断层摄影术三维测量耳廓再造术模板设计

THEORETICAL ANALYSES OF LIQUID-PHASE MASS TRANSFER IN WETTED-WALL COLUMN

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Science in China,Ser.A 1964年第12期 1987-2000页

作者：蒋国樑朱葆琳 Institute of Chemical Physics Academia Sinica

Ⅰ.INTRODUCTIONMass transfer process is one of the most important constituents in modern *** has been widely adopted in various departments of *** study ofmass transfer has in recent years aroused great interest,for i... 详细信息

关键词： THEORETICAL ANALYSES OF LIQUID-PHASE MASS TRANSFER IN WETTED-WALL COLUMN

Use of whole blood directly for single-cell gel electrophoresis （comet） assay of DNA strand breaks both in vivo and in vitro

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Use of whole blood directly for single-cell gel electrophore...

大孔吸附树脂分离纯化鲜地黄叶中梓醇的初步研究

第六届全国自由基生物学与自由基医学学术会议和海峡两岸自...

作者： Cheng-Hung chuang and Miao-Lin Hu Department of Food Science, chung-Hsing University, 250 kuo-kuang Raod, Taichung, 402 Taiwan,

＜正＞ The present study investigated the use of whole blood from human and rats directly for single-cell gel electrophoresis （comet） assay of DNA strand breaks. As little as 20 μL of whole blood was sufficient for comet assay, and the comet images obtained from whole blood were not different from those obtained from isolated lymphocytes. The DNA remained intact up to 4 h at 4℃after isolation and had no observable strand breakage when whole blood was cryopreserved （at -80℃） in 10% pre-cooled DMSO up to 60 days. To demonstrate that the whole blood technique could be applied to in vivo studies, we injected rats with a known carcinogen Fe/NTA and measured DNA strand breaks in whole blood in comparison with isolated lymphocytes. We showed that Fe/NTA injection resulted in similar extent of DNA strand breakage in both whole blood and lymphocytes, indicating that whole blood method can be used for in vivo genotoxic studies. One disadvantage of the whole blood technique is that whole blood cannot be used for in vitro studies because of the interferences from red blood cell （RBC） components. However, this problem can be overcome by prior hemolysis of RBCs and a brief centrifugation to obtain white blood cells （WBCs）, which can then be used for in vitro incubation with genotoxic compounds before comet assay. Overall, this whole-blood technique for comet assay is expected to provide a simple, rapid and cost-effective alternative for the existing comet assay using isolated lymphocytes in situations such as when time and cost are limiting factors.

关键词： Comet assay whole blood lymphocytes white blood cells cryopreservation.

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军事医学科学院院刊 2009年第6期33卷 550-553页

作者：匡岩巍卢兖伟吴祖泽军事医学科学院放射与辐射医学研究所北京100850

目的:选择合适的大孔吸附树脂对鲜地黄叶中梓醇进行富集纯化。方法:以高效液相为检测手段,梓醇含量为检测指标。采用吸附和解吸试验考察了12种大孔吸附树脂对梓醇的吸附解吸性能,筛选出H103树脂进行了分离纯化研究。结果:H103树脂对梓... 详细信息

目的:选择合适的大孔吸附树脂对鲜地黄叶中梓醇进行富集纯化。方法:以高效液相为检测手段,梓醇含量为检测指标。采用吸附和解吸试验考察了12种大孔吸附树脂对梓醇的吸附解吸性能,筛选出H103树脂进行了分离纯化研究。结果:H103树脂对梓醇具有很好的吸附解吸性能,其纯化条件为上样液浓度1mg/ml,上样体积2BV,上样吸附速度1～2BV/h,洗脱速度1～3BV/h,10%乙醇洗脱8BV。结论:采用H103树脂分离纯化鲜地黄叶中梓醇,可简化步骤,降低成本,方法简单可行。

关键词：地黄属梓醇大孔吸附树脂分离纯化