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Food Science and Human Wellness 2024年第3期13卷 1513-1530页

作者： Mingzhi Zhu Bohao Shang Fang Zhou Yong Yuan Feiyan Yin Jin Cao Jianan Huang kunbo wang Xin Zeng Maiquan Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China College of Life Sciences Huaibei Normal UniversityHuaibei 235000China Hunan Tea Group Co. Ltd.Changsha 410128China School of Food Science and Technology Hunan Agricultural UniversityChangsha 410128China

Golden-flower fungus,the only dominant microorganism determining the Fu-brick tea quality through fermentation and the important microbe in Liupao tea,is considered a potential probiotic fungus based on its anti-obesity ***,the classification of golden-flower fungi is still controversial;the anti-obesity effect of golden-flower fungus polysaccharides remains *** this study,we identify a golden-flower strain as Aspergillus cristatus based on morphological characteristics and multigene phylogeny analysis,which resolves the controversy of ***,we find *** polysaccharides(ACPS)attenuate obesity in *** modulate gut bacterial *** which Akkermansia,Akkermansia muciniphila,Bacteroides,Romboutsia,Blautia,and Desulfovibrio are considered the core microbes regulated by *** increase fecal total short-chain fatty acid content and serum,hepatic,and fecal total bile acid ***,ACPS-induced gut microbiota alteration plays a causal role in the protection from obesity,according to a fecal transplantation ***,ACPS ameliorate obesity by regulating gut microbiota and gut microbiota-related metabolites.

关键词： Golden-flower fungus Aspergillus cristatus Polysaccharides Obesity Gut microbiota

The exponential convergence of the CR Yamabe flow

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Science China Mathematics 2020年第5期63卷 979-992页

作者： Weimin Sheng kunbo wang School of Mathematical Sciences Zhejiang UniversityHangzhou 310027China College of Sciences China Jiliang UniversityHangzhou 310018China

In this paper,we study the CR(Cauchy-Riemann)Yamabe flow with zero CR Yamabe *** use the CR Poincaréinequality and a Gagliardo-Nirenberg type interpolation inequality to show that this flow has the long time solution... 详细信息

关键词： CR geometry CR Yamabe problem CR Yamabe flow CR Yamabe invariant

基于CiteSpace的黑茶研究现状可视化分析

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食品工业科技 2024年第8期45卷 397-406页

作者：张旭雯刘岁赵锦琦杨亚葛炳钢王坤波傅冬和湖南农业大学茶学教育部重点实验室湖南长沙410128 国家植物功能成分利用工程技术研究中心湖南长沙410128 湖南省植物功能成分利用协同创新中心湖南长沙410128 农业农村部园艺作物基因资源评价利用重点实验室湖南长沙410128

黑茶是最受欢迎的茶类之一,为梳理黑茶研究进程,本文利用CiteSpace可视化分析软件,基于中国知网(China national knowledge infrastructure,CNKI)数据库和Web of Science(WOS)数据库核心集合,筛选出的3436篇中文文献和617篇英文文献,对... 详细信息

黑茶是最受欢迎的茶类之一,为梳理黑茶研究进程,本文利用CiteSpace可视化分析软件,基于中国知网(China national knowledge infrastructure,CNKI)数据库和Web of Science(WOS)数据库核心集合,筛选出的3436篇中文文献和617篇英文文献,对作者、关键词、研究机构、国家以及发表期刊等信息进行可视化分析。结果表明,黑茶文献数量呈上升趋势,中文研究早于英文研究,正处于高速发展阶段;《福建茶叶》和《Journal of Agricultural and Food Chemistry》分别是发表黑茶文献最多的中英文期刊;黑茶研究形成了多个研究团体,学者代表有刘仲华、吕才有、周红杰等;研究机构主要为各大农业院校和茶叶研究所,其中,研究成果较深入的机构是云南农业大学、湖南农业大学和安徽农业大学;黑茶研究领域中,我国是影响力最大的国家,其次为美国和日本;中文研究侧重于黑茶品质形成机理、微生物、香气成分等方面,英文研究更关注黑茶的药理研究。黑茶对糖尿病、动脉粥样硬化等代谢疾病的干预功效研究仍是未来国内外研究的热点。

关键词：黑茶 CiteSpace 文献计量学科学知识图谱

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不同产地茯砖茶的品质、抗氧化性比较及“金花”菌形态特征分析

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食品工业科技 2024年第6期45卷 272-279页

作者：马荣蓉冉莉莎屈丽池谢念祠丁梦梅朱洺志王坤波湖南农业大学园艺学院茶学教育部重点实验室湖南长沙410128 国家植物功能成分利用工程技术研究中心湖南长沙410128 植物功能成分利用省部共建协同创新中心湖南长沙410128 农业农村部园艺作物基因资源评价利用重点实验室湖南长沙410128 湖南农业大学黄埔创新研究院湖南长沙410128

本研究以湖南省、湖北省、陕西省、浙江省和贵州省5个不同产地茯砖茶为研究对象,分别对其化学成分、品质特征、抗氧化活性及“金花”菌形态特征进行比较分析。结果显示,5个不同产地茯砖茶之间化学成分含量存在显著差异(P<0.05)。其中贵... 详细信息

本研究以湖南省、湖北省、陕西省、浙江省和贵州省5个不同产地茯砖茶为研究对象,分别对其化学成分、品质特征、抗氧化活性及“金花”菌形态特征进行比较分析。结果显示,5个不同产地茯砖茶之间化学成分含量存在显著差异(P<0.05)。其中贵州茯砖茶游离氨基酸、总儿茶素含量最高(9.03、63.12 mg/g)。浙江茯砖茶茶多酚和黄酮含量最高(132.93、8.63 mg/g)。滋味属性显示湖南茯砖苦味最强,陕西茯砖茶涩味最强,浙江茯砖茶苦味回味和咸味最强,贵州茯砖茶鲜味和丰富度最强。不同茯砖茶的抗氧化活性有差异,其中湖北和浙江茯砖茶抗氧化活性较高。相关性分析表明茯砖茶的主要化学成分与滋味属性显著正相关(P<0.05),茶多酚对茯砖茶的抗氧化活性有贡献。5株“金花”菌在相同培养基上形态差异相对较小,湖南菌株生长速度快于其它菌株。本研究对解析不同产地茯砖茶的化学品质具有重要意义。

关键词：茯砖茶不同产地品质特征抗氧化活性金花菌形态

2019-2021年山西省长治市居民期望寿命及去死因期望寿命分析

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疾病监测 2024年第3期39卷 376-381页

作者：王家钰李慕帆杜中强王鲲博饶华祥杨建洲山西医科大学公共卫生学院山西太原030001 长治市疾病预防控制中心山西长治046000 中南大学公共卫生学院湖南长沙410000 长治医学院公共卫生与预防医学系山西长治046000

目的分析2019-2021年山西省长治市居民死亡情况及期望寿命变化趋势,为制定相关防治策略提供参考依据。方法收集整理2019-2021年长治市死因监测数据,结合漏报情况对死亡数据进行漏报调整。应用期望寿命表和期望寿命分解法对2019-2021... 详细信息

目的分析2019-2021年山西省长治市居民死亡情况及期望寿命变化趋势,为制定相关防治策略提供参考依据。方法收集整理2019-2021年长治市死因监测数据,结合漏报情况对死亡数据进行漏报调整。应用期望寿命表和期望寿命分解法对2019-2021年长治市居民死因监测数据进行分析,计算不同年龄、不同疾病死亡率对期望寿命的贡献值。结果 2019-2021年长治市居民期望寿命分别为75.11、76.22和76.25岁,男性期望寿命分别为73.22、74.34和74.12岁,女性期望寿命分别为77.30、78.34和78.74岁。相对于2019年,2021年居民期望寿命增加了1.14岁,男性期望寿命增加了0.90岁,女性期望寿命增加了1.44岁。心脑血管疾病和肿瘤导致的寿命损失最多,其次是损伤和中毒、呼吸系统疾病。85岁及以上年龄组对居民期望寿命增加“正向贡献”最大,期望寿命增量为0.39岁,贡献率为34.20%。45~岁组对期望寿命增加“负向贡献”最大,期望寿命增量为-0.07岁,贡献率为-5.95%。结论心脑血管疾病、肿瘤、损伤和中毒以及呼吸系统疾病是导致长治市居民人均期望寿命损失的主要原因,45~岁组是影响长治市居民期望寿命的主要年龄组,在中老年人群中开展健康干预是今后该地区提高居民健康水平、降低疾病负担的重点措施。

关键词：期望寿命去死因期望寿命死亡率期望寿命分解法

Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH

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Journal of Integrative Plant Biology 2013年第7期55卷 654-662页

作者： Qiong Wu Fang Liu Shaohui Li Guoli Song Chunying wang Xiangdi Zhang Yuhong wang David Stelly kunbo wang Cotton Research Institute the Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Biology Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Department of Soil and Crop Sciences Texas A&M UniversityCollege Station

Gossypium mustelinum （（AD）4） is one of five disomic species in Gossypium. Three 45S ribosomal DNA （rDNA） loci were detected in （AD）4 with 45S rDNA as probe, and three pairs of brighter signals were detected with genomic DNA （gDNA） of Gossypium D genome species as probes. The size and the location of these brighter signals were the same as those detected with 45S rDNA as probe, and were named GISH-NOR. One of them was super-major, which accounted for the fact that about one-half of its chromosome at metaphase was located at chromosome 3, and other two were minor and located at chromosomes 5 and 9, respectively. All GISH-NORs were located in A sub-genome chromosomes, separate from the other four allopolyploid cotton species. GISH-NOR were detected with D genome species as probe, but not A. The greatly abnormal sizes and sites of （AD）4 NORs or GISH-NORs indicate a possible mechanism for 45S rDNA diversification following （AD）4 speciation. Comparisons of GISH intensities and GISH-NOR production with gDNA probes between A and D genomes show that the better relationship of （AD）4 is with A genome. The shortest two chromosomes of A sub-genome of G. mustelinum were shorter than the longest chromosome of D sub-genome chromosomes. Therefore, the longest 13 chromosomes of tetraploid cotton being classified as A sub-genome, while the shorter 13 chromosomes being classified as D sub-genome in traditional cytogenetic and karyotype analyses may not be entirely correct.

关键词： Chromosomes cotton Gossypium mustelinum in situ hybridization nucleolar organizer region genome.

Primary analysis of QTG contribution to heterosis in upland cotton

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Chinese Science Bulletin 2010年第26期55卷 2956-2965页

作者： ZHANG XianLiang LIU Fang wang Wei LI ShaoHui wang ChunYing ZHANG XiangDi wang YuHong wang kunbo Cotton Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture of People's Republic of China Anyang 455000 China Kaifeng Research Academy of Agriculture and Forestry Key Laboratory of Agriculture Biological Breeding Kaifeng 475141 China Yanhai District Agricultural Research Institute of Jiangsu Province Yancheng 224002 China

In this paper, we analyzed the contribution of pure DNA factors to heterosis using quantitative trait genes (QTG) in two randomly selected strains from a recombinant inbred line of Gossypium hirsutum. According to a set of QTL mapping results, combined with analysis of DNA recombinant fragment sources in the two strains and QTL association analysis with their field traits, we hypothesize a view of "dominance + overdominance + epistasis". That is, additive and additive epistasis may be the genetic basis of heterosis, and dominance, overdominance and epistasis may be the modes of heterosis action. Based on the heterosis results of this study, we also suggest a molecular mechanism for heterosis, and explain, in detail, with two randomly selected strains as examples. The male and female parent-derived additive epistatic QTLs of upper half mean length in LG01 and LG03 produced a trait variance of 2.99-3.52 compared with the female parent-derived loci. The trait of bolls per plant was controlled by two pairs of additive epistatic QTLs in LG02 and LG07, which were derived from both female and male parents. The QTLs were reciprocally interacted and produced a trait variance of 0.86. An initial concept of "super-hybrid cotton" was raised according to the nature of additive effect, that is genetic stability.

关键词：杂种优势利用陆地棉 QTL定位数量性状基因重组自交系显性上位遗传基础随机选择

Designations for individual genomes and chromosomes in Gossypium

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Journal of Cotton Research 2018年第1期1卷 35-39页

作者： wang kunbo WENDEL Jonathan F. HUA Jinping State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Sciences Department of Ecology Evolution and Organismal Biology Iowa State University College of Agronomy and Biotechnology China Agricultural University

Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.

关键词： Nomenclature Individual genome Individual chromosome Gossypium

Aspergillus cristatus LJSC.2006的分离鉴定及对茯茶发花品质的影响

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茶叶科学 2024年第4期44卷 639-654页

作者：肖娟娟程滢刘雁刘巧芳蒋阿婷黄建安王坤波刘仲华王贞红禹利君湖南农业大学茶学教育部重点实验室湖南长沙410128 西藏农牧学院资源与环境学院西藏林芝860000 益阳市产商品质量监督检验研究院湖南益阳413099 国家植物功能成分利用工程技术研究中心湖南长沙410128 植物功能成分利用省部共建协同创新中心湖南长沙410128 农业农村部园艺作物基因资源评价利用重点实验室湖南长沙410128

为探究自行分离纯化菌株LJSC.2006对湖南茯茶主要黑毛茶发花品质的影响,综合菌落平皿形态、孢子电镜扫描特征、菌丝体分子标记鉴定结果,确定菌株LJSC.2006为冠突曲霉菌(Aspergillus cristatus LJSC.2006,GenBank登录号:MZ147025)。运用... 详细信息

为探究自行分离纯化菌株LJSC.2006对湖南茯茶主要黑毛茶发花品质的影响,综合菌落平皿形态、孢子电镜扫描特征、菌丝体分子标记鉴定结果,确定菌株LJSC.2006为冠突曲霉菌(Aspergillus cristatus LJSC.2006,GenBank登录号:MZ147025)。运用感官审评、生化成分分析、HS-SPME/GC-MS挥发性组分分析等方法比较黑毛茶及其发花茶样的滋味、香气品质,发现黑毛茶发花后干茶色泽加深,金花满披,菌香显露,滋味醇和;滋味成分黄酮、茶多酚、可溶性糖、游离氨基酸、酯型儿茶素、杨梅素、槲皮素含量总体呈下降趋势;香气成分增加,醛类、酯类相对含量增多,苯乙烯和雪松醇为黑毛茶共有香气成分,(E)-芳樟醇-3,7-氧化物和苯乙酮为发花茶样共有香气成分;黑毛茶发花后,水杨酸甲酯、(E,E)-2,4-庚二烯醛、(E)-芳樟醇-3,7-氧化物、(E)-呋喃氧化芳樟醇、(E)-2-壬醛、(E)-2-已烯醛、(E)-2-(Z)-6-壬二烯醛、苯乙酮、(E)-2-壬烯醛及香叶酸甲酯等10种特征性挥发成分相对含量明显上升,综合形成了金花散茶的菌花香。

关键词：冠突曲霉菌菌种鉴定黑毛茶金花散茶发花品质

茶树响应高温胁迫下生理生化和分子机制的研究进展

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中国茶叶 2024年第3期46卷 26-31页

作者：林俊铭赵体跃黄翔翔赵剑王坤波湖南农业大学茶学教育部重点实验室湖南长沙410128 湖南农业大学国家植物功能成分利用工程技术研究中心湖南长沙410128 湖南农业大学农业农村部园艺作物基因资源评价利用重点实验室湖南长沙410128 湖南农业大学黄埔创新研究院广东广州510700

茶树作为一种重要的经济作物,具有喜温、喜湿、喜阴的生态特性。然而,高温胁迫会影响茶树的生长发育,显著降低茶叶的产量和品质。文章综述了高温胁迫对茶树生理及重要次生代谢产物(儿茶素、茶氨酸、咖啡碱)影响的研究进展,并阐述了茶树... 详细信息

茶树作为一种重要的经济作物,具有喜温、喜湿、喜阴的生态特性。然而,高温胁迫会影响茶树的生长发育,显著降低茶叶的产量和品质。文章综述了高温胁迫对茶树生理及重要次生代谢产物(儿茶素、茶氨酸、咖啡碱)影响的研究进展,并阐述了茶树响应高温的分子机制,以期为提高茶树栽培措施和选育耐热茶树品种提供理论依据。

关键词：茶树高温响应次生代谢热休克转录因子