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Reference Gene Selection for Quantitative Real-Time PCR Analyses of Acer palmatum under Abiotic Stress

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Phyton-International Journal of Experimental Botany 2020年第2期89卷 385-403页

作者： Lu Zhu Qiuyue Ma Shushun Li Jing Wen kunyuan Yan Qianzhong Li Institute of Leisure Agriculture Jiangsu Academy of Agricultural SciencesNanjing210014China

Quantitative real-time reverse transcriptase PCR(qRT-PCR)technology has been extensively used to estimate gene expression levels,and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized *** palmatum is an important colorful leaf ornamental tree species,and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been *** this study,the expression stability of ten candidate reference genes,namely,Actin3,Actin6,Actin9,EF1α,PP2A,SAMDC,TIP41,TUBα,TUBβand UBQ10,in two distinct tissues(leaves and roots)of *** under four different abiotic stress conditions(cold,heat,salt and drought)were investigated and assessed using three statistical methods(GeNorm,NormFinder and BestKeeper).The combinations of reference genes that showed stability in the different stressed samples ***,Actin6,UBQ10 and Actin9 were the most stable reference genes in all the samples,and Actin3 and Actin6 wexre stably expressed in cold-stressed leaves and ***3,Actin6 and UBQ10 were identified as an appropriate combination of reference genes for the analysis of heat-stressed leaves and roots,whereas the combination of Actin9,UBQ10 and Actin6 was deemed the most suitable for the analysis of salt-stressed leaves and ***,Actin6 and UBQ10 exhibited stable expression in drought-stressed leaves and ***,the expression levels of CBF,Cu/Zn-SOD and HsfA1 were estimated to determine the reliability of the reference genes assessed in this *** study revealed stable reference genes in *** that might be used for the normalization of qRT-PCR data obtained under various abiotic stresses.

关键词： Acer palmatum qRT-PCR abiotic stresses referencegene normalization

GRAFT COPOLYMERIZATION OF ACRYLAMIDE ONTO POLY（N-PARA-TOLYLACRYLAMIDE） FILMS INITIATED BY CERIC ION

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Chinese Journal of Polymer Science 1988年第1期6卷 75-83页

作者：丘坤元宋叶 Department of Chemistry Peking University Beijing

Functional monomers having phenylcarbamoyl and p-tolylcarbamoyl were synthesized. Binary initiation system consisted of Ce4+ and these functional monomers could initiate acrylamide polymerization. It was found that acrylamide could graft copolymerize onto macromolecules having these functional monomers units. The formation of graft copolymer has been verified by its percentage of water absorption, contact angle with water, XPS and SEM photographs. The reaction mechanism was proposed and the main reactive site of graft ocpolymerization initiated by Ce4+ would take place predominantly at the functional pendant group.

关键词： Acrylamide Functional monomers N-para-tolylacrylamide Copolymers Graft copolymerization initiated by ceric ion Characterization of grafted copolymers by SEM XPS.

Prophylaxis of tumor through oral administration of IL-12 GM-CSF gene carried by live attenuated salmonella

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Chinese Science Bulletin 2001年第13期46卷 1107-1112页

作者： LI Yuhua CHEN Hui QUO kunyuan XIE Yongmei WANG Feng XIE Kuangcheng HUANG Jiangsheng REN Darning Department of Hematology Zhujiang HospitalFirst Military Medical UniversityGuangzhou 510282China Institute of Genetics School of Life ScienceSlate Key Laboratory of Genetic EngineeringFudan UniversityShangshai 200433China Central Research Lab of First Hospital of Shanghai Shangshai 200000China

A live attenuated AraA autotrophic mutant of Salmonella typhimurium (SL3261) was used as carrier for eukaryotic expression vectors EGFPN1, pCMVmIL-12, pCMVWL-12, pCMVmGM-CSF and PCMVhGM-CSF and was administered orally to BALB/c and C57BL/6 mice. After 6 weeks, these mice were challenged with 4T1 and Lewis tumor cells respectively. GFP expression and gene integration could be detected in mice’s livers, spleens, intestines, kidneys and tumors. The serum level of cytokines increasedsignificantly in treated mice, so did the ratio of CD8+/CD4+,which resulted in the tumor regression and prolongation of the survival time of those mice. These researches laid an experimental foundation for the tumor gene therapy using live attenuated salmonella.

关键词： oral administration gene therapy attenuated salmonella.

Electronic Ratchet Nanodiodes

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Engineering（科研） 2009年第2期1卷 127-131页

作者： kunyuan XU Gang WANG 不详

Using a two-dimensional ensemble Monte Carlo (EMC) method, electronic nanometer devices with different parameters are studied in detail. Calculation results show that at nanoscale the electric properties of interface inside the devices play an important role in determining the working properties of the devices. By properly arranging device structures, surface charges originated from device fabrication can be exploited to produce a predetermined electric potential in the devices. Based on this fact, two structures that can lead to an asymmetric potential along their nanochannel are proposed for designing strong nonlinear devices. Further studies indicate that Ratchet effect brought by the asymmetric potential results in diode-like current-voltage characteristics of the devices. Through optimizing device parameters, zero threshold voltage can be achieved, which is desired for detecting applications. Moreover, since the devices are at nanoscale, simulation results reveal that used as rectifiers the working frequencies can be up to a few THz.

关键词： Ratchet Effect Nanodiode Asymmetric Monte Carlo

基于弯曲激波压缩系统的高超声速进气道反设计研究进展

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航空学报 2015年第1期36卷 274-288页

作者：张堃元南京航空航天大学能源与动力学院江苏省航空动力系统重点实验室南京210016

总结了近十年来弯曲激波压缩研究的主要成果。提出了弯曲激波压缩系统的新概念,即利用特殊设计的楔形弯曲压缩面或空间弯曲压缩面,产生一系列与前缘弱激波相互交汇或叠加的压缩波系,从而使前缘激波弯曲,形成特殊的弯曲激波,它与波后的... 详细信息

总结了近十年来弯曲激波压缩研究的主要成果。提出了弯曲激波压缩系统的新概念,即利用特殊设计的楔形弯曲压缩面或空间弯曲压缩面,产生一系列与前缘弱激波相互交汇或叠加的压缩波系,从而使前缘激波弯曲,形成特殊的弯曲激波,它与波后的等熵压缩波来共同完成对气流的压缩。在此基础上,实现了由给定出口气动参数的超声速内流道反设计,实现了由给定压缩面压力分布和给定压缩面马赫数分布要求的型面反设计,实现了由给定激波波面的压缩型面反设计。研究证明,弯曲压缩面-弯曲激波压缩系统具有良好的综合气动性能,为高性能高超声速进气系统的气动设计提供了一种全新的设计方法。

关键词：高超声速进气道反设计弯曲激波压缩系统弯曲压缩面风洞试验变几何进气道

STUDIES OF THE INITIATION MECHANISM OF VINYL POLYMERIZATION WITH THE SYSTEM PERSULFATE/N-ALKYL SUBSTITUTED ETHYLENEDIAMINE DERIVATIVES

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Chinese Journal of Polymer Science 1989年第2期7卷 165-173页

作者：郭新秋丘坤元冯新德 Department of Chemistry Peking University

Effects of N-alkyi substituted ethylenediamine derivatives on vinyl polymerization using persulfate as initiator were studied. The apparent kinetic equations and overall activation energies of acrylamide polymerization were determined using the above mentioned system as initiator. The promoting activities of different diamine derivatives on vinyl polymerization are in the order of tertiary diamine>secondary diamine>primary diamine. Diamines having methyl groups as the substituent on their nitrogen atom possess higher promoting activity than that of having larger alkyl groups. The initial free radicals produced through the redox reaction of persuifate and diamines were studied by spin strapping technique and ESR spectroscopy. The results obtained confirm the fact that the initial free radicals of the diamine species can initiate vinyl polymerization and become the amino end group of the resulting polymers.

关键词： Persulfate Ethylenediamine Derivatives ESR Acrylamide Radical Polymerization Redox System

Monsoon boosted radiation of the endemic East Asian carps

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Science China(Life Sciences) 2023年第3期66卷 563-578页

作者： Chenguang Feng Kun Wang Wenjie Xu Liandong Yang kunyuan Wanghe Ning Sun Baosheng Wu Feixiang Wu Lei Yang Qiang Qiu Xiaoni Gan Yiyu Chen Shunping He State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China School of Ecology and Environment Northwestern Polytechnical UniversityXi'an 710072China Key Laboratory of Adaptation and Evolution of Plateau Biota of Chinese Academy of Sciences Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810008China University of Chinese Academy of Sciences Beijing 100039China Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044China Florida Museum of Natural History University of FloridaGainesvilleFL 32611USA National Natural Science Foundation of China Beijing 100085China Center for Excellence in Animal Evolution and Genetics Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China

Major historical events often trigger the rapid flourishing of a few lineages,which in turn shape established biodiversity *** did this process occur and develop?This study provides a window into this *** endemic East Asian carps(EEAC)dominated the ichthyofauna of East Asia and exhibited a high degree of adaptation to monsoonal river-lake ecosystems.A series of evidence,including ecogeography,phylogenetics,and macroevolution,suggests that the EEAC is a lineage that arose with the East Asian monsoon and thrived intimately with subsequent monsoon *** further deduce the evolution of the EEAC and find that a range of historical events in the monsoon setting(e.g.,marine transgression and regression and glacial-interglacial cycle)have further reshaped the distribution patterns of EEAC’s *** genomics analyses reveal that introgressions during the initial period of EEAC radiation and innovations in the regulation of the brain and nervous system may have aided their adaptation to river-lake ecosystems in a monsoon setting,which boosted ***,this study strengthens knowledge of the evolutionary patterns of freshwater fishes in East Asia and provides a model case for understanding the impact of major historical events on the evolution of biota.

关键词： adaptive radiation Xenocyprididae east Asian monsoon genomes macroevolution phylogenomics