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Induction of the apoptosis of cancer cell by sonodynamic therapy:a review

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Chinese Journal of Cancer Research 2012年第4期24卷 368-373页

作者： wen-kun Bai E Shen Bing Hu Department of Ultrasound In Medicine Shanghai Jiaotong University Affiliated 6th People's HospitalShanghai Institute of Ultrasound in Medicine Department of Ultrasound in Medicine Shandong University Affiliated Qian Fo Shan Hospital

Ultrasound can be used not only in examination, but also in therapy, especially in the therapy of cancer. Sonodynamic therapy is an experimental cancer therapy method which uses ultrasound to enhance the cytotoxic effects of agents known as sonosensitizers. It has been tested in vitro and in vivo. The ultrasound could penetrate the tissue and cell under some of conditions which directly changes cell membrane permeability, thereby allowing the delivery of exogenous molecules into the cells in some degree. Ultrasound could inhibit the proliferation or induce the apoptosis of cancer cells in vitro or in vivo. Recent researches indicated low-frequency and low-intensity ultrasound could induce cell apoptosis, which could be strengthened by sonodynamic sensitivity, microbubbles, chemotherapeutic drugs and so on. Most kinds of ultrasound suppressed the proliferation of cancer cells through inducing the apoptosis of cancer cells. The mechanism of apoptosis is not clear. In this review, we will focus on and discuss the mechanisms of the induction of cancer cell apoptosis by ultrasound.

关键词： Sonodynamic therapy apoptosis cancer mechanism

Middle Devonian ostracods from Jiwozhai patch reef(Dushan,Guizhou Province,South China):Biostratigraphic and palaeoecological implication

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Journal of Palaeogeography 2024年第3期13卷 581-593页

作者： Jun-Jun Song Jia-Yuan Huang wen Guo kun Liang Yu-Cong Sun Zhi-Hong Wang wen-kun Qie State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008Jiangsu ProvinceChina Hubei Key Laboratory of Paleontology and Geological Environment Evolution Wuhan Center of China Geological SurveyWuhan 430205Hubei ProvinceChina University of Chinese Academy of Sciences NanjingNanjing 211135Jiangsu ProvinceChina

The Middle Devonian ostracods from the Jiwozhai patch reef in Dushan, Guizhou Province, South China were described for the first time in this study. Twenty-nine species belonging to 20 genera were identified and figured, including a new species named Wangshangkia jiwozhaiensis sp. nov. The ostracod fauna in the Jiwozhai patch reef represents a Givetian age. The ostracod assemblage belongs to the smooth-podocopid association, which is also ecologically equivalent to the Eifelian Mega-Assemblage Ⅲ. This association is indicative of shallow open-marine environments. The Jiwozhai patch reef fauna is primarily comprised of suspension feeders, including filter-feeding ostracods, corals, brachiopods and bryozoans. Ostracods from the Jiwozhai patch reef were compared to the ostracod faunas from Laurentia-Baltica at generic level, which reveals limited faunal exchanges between South China and Laurentia-Baltica during the Middle Devonian.

关键词： Ostracods Givetian Jiwozhai patch reef Dushan Palaeoecology

Delayed diagnosis of ascending colon mucinous adenocarcinoma with local abscess as primary manifestation: Report of three cases

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World Journal of Clinical Cases 2021年第26期9卷 7901-7908页

作者： Shang-Zhi Han Rong Wang kun-Ming wen Department of Gastrointestinal Surgery Affiliated Hospital of Zunyi Medical UniversityZunyi 563000Guizhou ProvinceChina

BACKGROUND Colorectal mucinous adenocarcinoma is a distinct subtype of colorectal adenocarcinoma that is not sensitive to chemotherapy and radiotherapy,and its prognosis is worse than that of nonmucinous *** diagnosis and aggressive surgical treatment may be the key to improving the prognosis of *** colon mucinous adenocarcinoma with the primary manifestation of a local abscess caused by non-intestinal perforation has never been ***,since the lumen of the ascending colon is large,and early stage ascending colon cancer lacks typical clinical manifestations,the diagnosis may be delayed *** herein report three cases of delayed diagnosis of colorectal mucinous *** SUMMARY We present three patients(two females and one male)with mucinous ascending colon mucinous adenocarcinoma with the primary manifestation of a local abscess(the right area of the lumbar spine,right groin,and lower right abdomen)caused by non-intestinal *** the initial clinical visit,the common causes of those abscesses,including spinal tuberculosis and urinary tract infection,were *** treatment of the abscess was through an incision and ***,the source of the abscess was not made clear,which led to an abscess recurrence and a delayed diagnosis of colorectal mucinous *** the patients were referred to our hospital,a definitive diagnosis of ascending colon mucinous adenocarcinoma was made with the help of tumor markers and colonoscopic *** of the delayed diagnosis of the disease,two patients(case 1 and case 2)missed the chance of surgery due to disease progression and died in a short follow-up *** case 3 underwent radical surgery for the tumor in the right colon and partial abdominal wall resection and achieved a better *** Abscesses in the right area of the lumbar spine,right groin,or right lower quadrant caused by non-intestinal perforation as the prima

关键词： Delayed diagnosis Colorectal mucinous adenocarcinoma Local abscess Case report

AtCLH2, a Typical but Possibly Distinctive Chlorophyllase Gene in Arabidopsis

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Journal of Integrative Plant Biology 2007年第4期49卷 531-539页

作者： Yang Liao kun An Xiao Zhou wen-Jun Chen Ben-Ke Kuai State Key Laboratory of Genetic Engineering Department of Biochemistry School of Life Sciences Fudan University Shanghai 200433 China

Chlorophyllase （EC 3.***.1.14） is involved in the first step of chlorophyll degradation. Isolation of chlorophyllase genes greatly facilitates characterization of chlorophyllase properties and elucidation of molecular regulation of their in vivo activities. There are two chlorophyllase genes, AtCLH1 and AtCLH2, in Arabidopsis thaliana. The in vivo roles of AtCLH1 have been reported previously. However, few studies have been carried out on AtCLH2. Here, we show that purified recombinant Chlase2, encoded by AtCLH2, exhibits in vitro chlorophyllase activity. Interestingly, ＂activation＂ of in vitro activity of the recombinant Chlase2 required higher concentrations of a detergent or a polar solvent. To determine its activity in vivo, the expression of AtCLH2 was inhibited by RNA interference. RNAi plants showed decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves. In addition, the two AtCLHs exhibited differential expression patterns. Our results suggest that AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo.

关键词： Arabidopsis AtCLH1 AtCLH2 chlorophyllase chlorophyll degradation RNA interference.

Coating of microbially produced calcium carbonate onto stone materials

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Science China(Technological Sciences) 2015年第2期58卷 266-272页

作者： ZHU wen kun MU Tao ZHANG You Kui DUAN Tao LUO Xue Gang Engineering Research Center of Biomass Materials Ministry of Education Southwest University of Science and Technology China Academy of Engineering Physics Joint Laboratory for Extreme Conditions Matter Properties Southwest University of Science and Technology and Research Center of Laser FusionCAEP

The coatings of microorganism-induced calcium carbonate onto the stone surface carried out by using both of the immersion method and coating method were investigated. Various analysis and testing techniques such as scanning electron micrograph （SEM） and X-ray diffraction （XRD） were used to characterize the deposited mineral layer. The adhesive property, acid re- sistance, frost resistance, light and aging resistance, water adsorption and permeability were investigated in detail. The results showed that both immersion method and coating method could produce calcium carbonate granules with sizes ranging from 1 to 10 pm and form a layer of dense mineralization membrane which is about 50 to 100 pm thick. Immersion method was more efficient than coating method. The large cohesive force between calcium carbonate layer and stone materials could improve the acid rain resistance as well as excellent heat tolerance, frost resistance and light aging resistance. The coating process could not only help the stone materials maintain its original permeability with the aid of calcium carbonate layers but also improve the penetration resistance significantly. Therefore, this type of technology shows a great potential in the protection of stone relics.

关键词： microorganism microbially induced deposited-layer stone materials

Design and Algorithm Research of a GNSS/FOG-SINS Integrated Navigation System for Unmanned Vehicles 36

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Design and Algorithm Research of a GNSS/FOG-SINS Integrated ...

Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene(Ha-acp1) from Heterodera avenae

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作者： Han Wang Maosong Wang kun wen wenqi Wu College of Mechatronics Engineering and Automation National University of Defense Technology The University of Calgary

ISBN: (纸本)9781538629185

According to the application requirements of autonomous navigation for unmanned vehicles which mainly used for forest exploration and border patrols,this paper designs a cost effective GNSS/SINS integrated system based on the fiber optic gyro（FOG）.After that,the alignment method of SINS and the integrated navigation algorithm are also *** fusing the valid data of GNSS/FOG-SINS with Kalman filter,the error of inertial devices can be corrected and the high-precision inertial navigation results are used to locate by the satellite navigation *** the end of this paper,the simulation and the vehicle field test are done,from which we can see that the tested accuracy can meet the practical precision requirement of the integrated navigation system when the satellite signal is blocked in the complex terrain environment for one minute.

关键词： Unmanned Vehicles GNSS/FOG-SINS Integrated Navigation Kalman Filter

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Journal of Integrative Agriculture 2014年第6期13卷 1303-1310页

作者： LIU Yan-ke HUANG wen-kun LONG Hai-bo PENG Huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词： Heterodera avenae acid phosphatase RNA interference

Regulation of Microstructure of Calcium Carbonate Crystals by Egg White Protein

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Chemical Research in Chinese Universities 2012年第2期28卷 180-185页

作者： ZHU wen-kun LUO Xue-gang ZHANG Chi DUAN Tao ZHOU Jian School of National Defense Science and Technology Southwest University of Science and TechnologyMianyang 621010P.R.China Engineering Research Center of Biomass Materials of Ministry of Education Southwest University of Science and TechnologyMianyang 621010P.R.China

Crystal growth of calcium carbonate in biological simulation was investigated via egg white protein with different volume fractions,during which calcium carbonate was synthesized by calcium chloride and sodium *** morphology,thermal properties and microstructure of the calcium carbonate micro-to-nanoscale crystals were characterized by scanning electron microscopy（SEM）,transmission electron microscopy（TEM）,Fourier transform infrared spectroscopy（FTIR）,thermogravimetric analysis（TG） and X-ray diffraction（XRD） *** results show that the volume fraction of egg white protein has great influence on the shape,size and morphology of calcium carbonate *** calcium carbonate crystals were the mixtures of calcite-vaterite-like crystals including spherical and rough surface,which are different from that formed in pure *** the increase of egg white protein concentration,the diameter of calcium carbonate crystals changed,the amount of formed spherical calcium carbonate particles decreased and that of vaterite *** results indicate that the coordination and electrostatic interaction between egg white protein and Ca2＋ significantly affect the calcium carbonate crystalization.

关键词： Egg white protein Spherical calcium carbonate Vaterite Calcite

CD137 costimulation enhances the antiviral activity of Vγ9Vδ2-T cells against influenza virus

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1746-1755页

作者： Yujun Pei kun wen Zheng Xiang Chunyu Huang Xiwei Wang Xiaofeng Mu Liyan wen Yinping Liu wenwei Tu Department of Paediatrics and Adolescent Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

Influenza epidemics and pandemics are constant threats to global public *** strategies including vaccines and antiviral drugs have achieved great advances in controlling influenza virus infection,the efficacy of these strategies is limited by the highly frequent mutations in the viral genome and the emergence of drug-resistant *** previous study indicated that boosting the immunity of human Vγ9Vδ2-T cells with the phosphoantigen pamidronate could be a therapeutic strategy to treat seasonal and avian influenza virus ***,one notable drawback ofγδ-T cell-based immunotherapy is the rapid exhaustion of proliferation and effector responses due to repeated treatments with ***,we found that the expression of CD137 was inducible in Vγ9Vδ2-T cells following antigenic ***137^(+)Vγ9Vδ2-T cells displayed more potent antiviral activity against influenza virus than their CD137−counterparts in vitro and in Rag2^(-/-)γc^(-/-)*** further demonstrated that CD137 costimulation was essential for Vγ9Vδ2-T cell activation,proliferation,survival and effector *** humanized mice reconstituted with human peripheral blood mononuclear cells,CD137 costimulation with a recombinant human CD137L protein boosted the therapeutic effects of pamidronate against influenza *** study provides a novel strategy of targeting CD137 to improve the efficacy of Vγ9Vδ2-T cell-based immunotherapy.

关键词： CD137 influenza drugs