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Comparative quantitative analysis of fruit oil from Hippophae rhamnoides(seabuckthorn) by qNMR, FTIR and GC–MS

Chinese Herbal Medicines 2023年第4期15卷 607-613页

作者： Dattatraya Dinkar Gore Furkan Ahmad kulbhushan tikoo Arvind Kumar Bansal Dinesh Kumar Inder Pal Singh National Institute of Pharmaceutical Education and Research(NIPER) S.A.S.Nagar 160062India CSIR-Institute of Himalayan Bioresource and Technology Palampur 176061India

Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides(seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and ***: SBT fruit oil(IPHRFH) was extracted with hexane and the triglyceride(TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers(Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR ***: The qNMR results showed that the oil contained 80.3% of triacylglycerol(TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier(SW) were fruit oil;All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed ***: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore,analysis of commercial samples showed discrepancies between label claims and actual content.

关键词： Hippophae rhamnoides L. omega fatty acids qNMR seabuckthorn triacylglycerols

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Traversing the advantageous role of samarium doped spinel nanoferrites for photocatalytic removal of organic pollutants

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Journal of Rare Earths 2021年第7期39卷 781-789,I0002页

作者： Sneha Singh Paramdeep Kaur Vinod Kumar K.B.tikoo Sonal Singhal Department of Chemistry Panjab UniversityChandigarh160014India HRTEM Facility Lab NIPERSA.S.NagarMohaliPunjab160062India

The present work reportes the pertinence of samarium(Sm) doped spinel nanoferrites as magnetically recoverable photocatalyst for the removal of organic pollutants from ***,a series of Sm substituted spinel nano ferrites,MSm_(x)Fe_(2-x)O_(4)(M=Ni,Co;x=0,0.02,0.06,0.1) we re synthesized via sol-gel *** effect of Sm doping on the structural,morphological,optical and magnetic properties of pristine nanoferrites was investigated ***,the fabricated samples were explored as photocatalysts for the oxidative degradation of antibiotics(ofloxacin and norfloxacin) and dyes(methyl orange and safranin O).The Sm doped nanoferrites exhibit astonishing catalytic efficacy that can be attributed to higher surface area,octahedral site preference of Sm ions and reduced band *** synthesized nanoferrites display excellent recyclability which enables them to be utilized as potential photocatalysts for wastewater treatment.

关键词： Samarium doped nanoferrites Sol-gel Pharmaceutical degradation Recyclability Rare earths

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Proteomic Analysis of Bovine Nucleolus

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Genomics, Proteomics & Bioinformatics 2010年第3期8卷 145-158页

作者： Amrutlal K.Patel Doug Olson Suresh K.tikoo Vaccine and Infectious Disease Organization University of Saskatchewan Department of Veterinary Microbiology Western College of Veterinary Medicine University of Saskatchewan National Research Council Plant Biotechnology Institute University of Saskatchewan School of Public Health University of Saskatchewan

Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.

关键词： bovine nucleolus proteomics 1D SDS-PAGE SCX LC-MS/MS

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Transgene IL-21-Engineered T Cell-Based Vaccine Potently Converts CTL Exhaustion via the Activation of the mTORC1 Pathway in Chronic Infection

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World Journal of Vaccines 2019年第1期9卷 1-21页

作者： Aizhang Xu Xueying Zhang Rajni Chibbar Andrew Freywald Suresh tikoo Changyu Zheng Jim Xiang Cancer Research Saskatchewan Cancer Agency Saskatoon Canada Department of Oncology College of Medicine University of Saskatchewan Saskatoon Canada Department of Pathology College of Medicine University of Saskatchewan Saskatoon Canada School of Public Health University of Saskatchewan Saskatoon Canada Molecular Physiology and Therapeutic Branch National Institute of Dental and Craniofacial Research National Institute of Health Bethesda USA

CD8+ cytotoxic T lymphocyte (CTL) exhaustion is one of the major obstacles for the effectiveness of virus control in chronic infectious diseases. We previously generated novel ovalbumin (OVA)-specific 41BBL-expressing OVA-TEXO and human immunodeficiency virus (HIV-1) Gag-specific Gag-TEXO vaccines, inducing therapeutic immunity in wild-type C57BL/6 (B6) mice, and converting CTL exhaustion in recombinant OVA-specific adenovirus AdVOVA-infected B6 (AdVOVA-B6) mice with chronic infection. IL-21 cytokine plays an important role in controlling chronic infections. Therefore, in this study, we constructed recombinant transgene IL-21-expressing AdVIL-21, and generated IL-21-expressing OVA-TEXO/IL-21 and Gag-TEXO/IL21 vaccines, or control vaccines (OVA-TEXO/Null and Gag-TEXO/Null) by infecting OVA-TEXO and Gag-TEXO cells with AdVIL-21 or the control AdVNull, lacking transgene, and assessed their effects in B6 or AdVOVA-B6 mice. We demonstrate that both OVA-TEXO/IL-21 and control OVA-TEXO/Null vaccines are capable of converting CTL exhaustion in chronic infection. However, the OVA-TEXO/IL-21 vaccine more efficiently rescues exhausted CTLs by increasing stronger CTL proliferation and effector cytokine IFN-γ expression than the control OVA-TEXO/Null vaccine in AdVOVA-B6 mice with chronic infection, though both vaccines stimulated comparable OVA-specific CTL responses and protective immunity against OVA-expressing BL6-10OVA melanoma lung metastasis in wild-type B6 mice. In vivo, the OVA-TEXO/IL-21-stimulated CTLs more efficiently up-regulate phosphorylation of mTORC1-controlled EIF4E and expression of mTORC1- regulated T-bet molecule than the control OVA-TEXO/Null-stimulated ones. Importantly, the Gag-TEXO/IL21 vaccine induces stronger Gag-specific therapeutic immunity against established Gag-expressing BL6-10Gag melanoma lung metastases than the control Gag-TEXO/Null vaccine in chronic infection. Therefore, this study should have a strong impact on developing new therapeutic va

关键词： IL-21 Chronic Infection CTL Exhaustion Exosome T Cell Vaccine

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科学大洋钻探的巨大变化

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世界科学 2024年第10期 20-25页

作者：李军平丽贝卡·罗宾逊索尼娅·蒂库帕特里克·富尔顿美国罗德岛大学斯坦福大学康奈尔大学

海底探索和发现的时代即将结束。然而，还有很多东西需要学习。由著名作家约翰·斯坦贝克（John Steinbeck）担任历史顾问，一群美国科学家和工程师于1961年开始执行一项史无前例的任务，即钻探大洋地壳并测量海底深处的温度。由于水域...

海底探索和发现的时代即将结束。然而，还有很多东西需要学习。由著名作家约翰·斯坦贝克（John Steinbeck）担任历史顾问，一群美国科学家和工程师于1961年开始执行一项史无前例的任务，即钻探大洋地壳并测量海底深处的温度。由于水域太深，无法抛锚，钻探小组使用了一艘经过特别改装的驳船，船上配备了一系列舷外发动机，使他们能够连续数周停留在同一地点。

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