Proteomic Analysis of Bovine Nucleolus

作     者：Amrutlal K.Patel Doug Olson Suresh K.Tikoo 

作者机构：Vaccine and Infectious Disease Organization University of Saskatchewan Department of Veterinary Microbiology Western College of Veterinary Medicine University of Saskatchewan National Research Council Plant Biotechnology Institute University of Saskatchewan School of Public Health University of Saskatchewan 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2010年第8卷第3期

页      面：145-158页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 071007[理学-遗传学] 0714[理学-统计学（可授理学、经济学学位）] 0703[理学-化学] 0901[农学-作物学] 0701[理学-数学] 090102[农学-作物遗传育种] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：University of Saskatchewan Natural Sciences and Engineering Research Council of Canada, NSERC 

主　　题：bovine nucleolus proteomics 1D SDS-PAGE SCX LC-MS/MS 

摘      要：Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.