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CYP1A1 , GSTM1 , GSTT1 and NQO1 polymorphisms and colorectal adenomas in Japanese men

World Journal of Gastroenterology 2013年第25期19卷 4023-4030页

作者： Tadamichi Hamachi Osamu Tajima kousaku uezono Shinji Tabata Hiroshi Abe Keizo Ohnaka Suminori Kono Department of Preventive Medicine Graduate School of Medical Sciences Kyushu University Self Defense Forces Fukuoka Hospital Kasuga-shi Self Defense Forces Kumamoto Hospital Department of Geriatric Medicine Graduate School of Medical Sciences Kyushu University

AIM: To investigate the role of functional genetic poly-morphisms of metabolic enzymes of tobacco carcinogens in the development of colorectal adenomas. METHODS: The study subjects were 455 patients with colorectal adenomas and 1052 controls with no polyps who underwent total colonoscopy in a preretirement health examination at two Self Defense Forces hospitals. The genetic polymorphisms studied wereCYP1A1*2A (rs 4646903), CYP1A1*2C (rs 1048943), GSTM1 (null or non-null genotype), GSTT1 (null or non-null genotype) and NQO1 C609T (rs 1800566). Genotypes were determined by the polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR method using genomic DNA extracted from the buffy coat. Cigarette smoking and other life-style factors were ascertained by a self-administered questionnaire. The associations of the polymorphisms with colorectal adenomas were examined by means of OR and 95%CI, which were derived from logistic regression analysis. Statistical adjustment was made for smoking, alcohol use, body mass index and other factors. The gene-gene interaction and effect modification of smoking were evaluated by the likelihood ratio test. RESULTS: None of the five polymorphisms showed a significant association with colorectal adenomas, nor was the combination of GSTM1 and GSTT1 . A borderline significant interaction was observed for the combination of CYP1A1*2C and NQO1 (P = 0.051). The OR associated with CYP1A1*2C was significantly lower than unity among individuals with the NQO1 609CC genotype. The adjusted OR for the combination of the CYP1A1*2C allele and NQO1 609CC genotype was 0.61 (95%CI: 0.42-0.91). Although the interaction was not statistically significant (P = 0.24), the OR for individuals carrying the CYP1A1*2C allele and GSTT1 null genotype decreased significantly compared with those who had neither CYP1A1*2C allele nor GSTT1 null genotype (adjusted OR: 0.69, 95%CI: 0.49-0.97). Smoking did not modify the associations of the individual

关键词： Colorectal adenoma Smoking Polymorphism CYP1A1 GSTM1 GSTT1 NQO1

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GM_2神经节苷脂沉积症发病的分子机理研究

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中华医学遗传学杂志 2003年第2期20卷 103-106页

作者：侯琳 Ohno kousaku 青岛大学医学院生化教研室 266021 日本鸟取大学医学部神经生物教室

目的　探讨GM2 神经节苷脂沉积症发病的分子机理。方法　培养患者皮肤成纤维细胞 ,进行酶学、Western印迹杂交和免疫细胞化学分析。结果　 4例GM2 神经节苷脂沉积症患者成纤维细胞内 β 氨基己糖苷酶 (hexosaminidase ,Hex)活性均显著降... 详细信息

目的　探讨GM2 神经节苷脂沉积症发病的分子机理。方法　培养患者皮肤成纤维细胞 ,进行酶学、Western印迹杂交和免疫细胞化学分析。结果　 4例GM2 神经节苷脂沉积症患者成纤维细胞内 β 氨基己糖苷酶 (hexosaminidase ,Hex)活性均显著降低 ,分别为正常人的 12 %、3 %、15 %和 6% ;两例Sandhoff病Hex成熟的α、β 亚基 (αm、βm)明显减少 ,但婴儿型与成年型减少的程度不同 ,1例Tay Sachs病只有αm明显减少 ,1例GM2 激活蛋白缺陷症αm、βm与正常人无差异 ;4例患者成纤维细胞内均有不同程度的GM2神经节苷脂的堆积。结论　GM2 神经节苷脂沉积症的发病与HEXA、HEXB、GM2 A基因突变引起相应蛋白表达、成熟障碍有关。

关键词： GM2神经节苷脂沉积症 β-氨基已糖苷酶亚基

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F213I突变型高雪氏病分子伴侣治疗方法的研究

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中华医学遗传学杂志 2003年第5期20卷 381-384页

作者：侯琳 Ohno kousaku 青岛大学医学院生化教研室 266021 日本鸟取大学医学部神经生物教室

目的　研究高雪氏病新的分子治疗方法。方法　用β-葡萄糖脑苷脂酶 (β-glucocerebrosidase,β-Glc) (EC3 .2 .1.45)的底物类似物 (glucocerebroside analogue,GCA)作为分子伴侣 ,处理体外培养的不同突变型的高雪氏病患者皮肤成纤维细... 详细信息

目的　研究高雪氏病新的分子治疗方法。方法　用β-葡萄糖脑苷脂酶 (β-glucocerebrosidase,β-Glc) (EC3 .2 .1.45)的底物类似物 (glucocerebroside analogue,GCA)作为分子伴侣 ,处理体外培养的不同突变型的高雪氏病患者皮肤成纤维细胞 ,采用荧光酶学手段测定β-Glc活性 ;Western印迹杂交技术分析该酶蛋白表达的量 ;细胞双染确定 β-Glc在细胞内的定位 ;薄层层析法分析葡萄糖脑苷脂的降解情况。结果该酶的底物类似物 GCA可以提高体外培养的 F2 13 I突变型患者成纤维细胞内 β-Glc活性 ;增加该酶蛋白的表达量 ;促进该酶蛋白向溶酶体内转运 ;加速底物葡萄糖脑苷脂 (glucocerebroside,Glc Cer)的降解。结论　低分子量的 GCA作为一种分子伴侣可能为 F2 13

关键词：高雪氏病治疗分子伴侣基因突变 β-葡萄糖脑苷脂酶 β-GLe

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Mechanism of Pb Removal from Brass Scrap by Compound Separation Using Ca and NaF

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International Journal of Nonferrous Metallurgy 2018年第1期7卷 1-7页

作者： Hidekazu Sueyoshi kousaku Yamada Masashi Miyazaki Takuya Okada Nobuyuki Ashie Yoshiharu Kousaka Graduate School of Science and Engineering Kagoshima University Kagoshima Japan Kyushu Tabuchi Co. Ltd. Kirishima Japan San-Etsu Metals Co. Ltd. Tonami Japan

The Mechanism of Pb removal from brass scrap by compound separation using Ca and NaF addition was investigated. Because large Ca-Pb compound particles formed by Ca addition rise to the surface of the molten brass, they can be skimmed off from the molten brass. However, fine Ca-Pb compound particles remain in the molten brass because of low buoyancy. By subsequent NaF addition, the reaction between Ca-Pb compound and NaF takes place at their contact regions, resulting in the formation of solid CaF2, liquid Pb and Na gas. Pb is mainly present at the Ca-Pb compound-CaF2 interface. CaF2 acts as a binder for aggregation of fine Ca-Pb compound particles, resulting in the formation of light and large composite compounds, which rise to the surface of the molten brass. A high Pb removal rate is achieved by skimming off.

关键词： Lead Removal Brass Scrap Calcium Sodium Fluoride Compound Separation

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Crystal Structure of Glycoside Hydrolase Family 78 α-L-Rhamnosidase from Bacillus sp. GL1

Crystal Structure of Glycoside Hydrolase Family 78 α-L-Rham...

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第十次全国环境微生物学术研讨会

作者： Yukie Mamyama Bunzo Mikami Wataru Hashimoto kousaku Murata

α-L-Rhamnosidase(EC 3.***.1.40)catalyzes the hydrolytic release of rhamnose from polysaccharides and *** ***1 α-L-rhamnosidase(RhaB),a member of glycoside hydrolase(GH)family 78,is responsible for degrading the bacterial biofilm gellan,and also functions as a debittering agent for citrus fruit in the food and beverage industries through the

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分子伴侣对戈谢病病人细胞内葡萄糖脑苷脂酶活性的影响

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青岛大学医学院学报 2003年第3期39卷 287-288,291页

作者：侯琳 Ohno kousaku 青岛大学医学院生物化学教研室山东青岛266021 日本鸟取大学医学部神经生物学教研室

①目的　探讨分子伴侣对戈谢病病人细胞内葡萄糖脑苷脂酶活性的影响。②方法　用 β 葡萄糖脑苷脂酶 (β Glc)的底物类似物 (GCA)作为分子伴侣 ,处理体外培养的不同突变型的戈谢病病人皮肤成纤维细胞 ,采用荧光酶学法检测 β Glc活性 ;W... 详细信息

①目的　探讨分子伴侣对戈谢病病人细胞内葡萄糖脑苷脂酶活性的影响。②方法　用 β 葡萄糖脑苷脂酶 (β Glc)的底物类似物 (GCA)作为分子伴侣 ,处理体外培养的不同突变型的戈谢病病人皮肤成纤维细胞 ,采用荧光酶学法检测 β Glc活性 ;Western印迹杂交技术分析该酶蛋白表达量 ;细胞双染法确定 β Glc在细胞内的定位。③结果　GCA可以提高体外培养的F2 13I突变型戈谢病病人成纤维细胞内 β Glc活性 ,增加酶蛋白的表达量 ,促进酶蛋白向溶酶体内转运。④结论　GCA有可能成为F2 13I突变型戈谢病治疗的一种新药物。

关键词：戈谢病分子伴侣细胞内葡萄糖脑苷脂酶治疗荧光酶学法 Western 印迹杂交技术

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Changes in Water Soluble Carbohydrates Content of Timothy (<i>Phleum pretense</i>L.) in Pastures of TMR Centers over 4 Years

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American Journal of Plant Sciences 2019年第2期10卷 276-284页

作者： Michiko Okubo Daisuke Takahashi Fumiya Abe Hideshi Shinoda Kenzaburo Deguchi Norie Shio Takayoshi Masuko kousaku Souma Faculty of Bioindustry Tokyo University of Agriculture Hokkaido Japan Snow Brand Seed Co. Ltd. Technical Research Institute Hokkaido Japan Animal Research Center Hokkaido Research Organization Hokkaido Japan Souya Agriculture Extension Office Hokkaido Agricultural Administration Hokkaido Japan Uvas Co. Ltd. Hokkaido Japan

Timothy (Phleum pretense L.) pastures of 3 Total mixed ration (TMR) centers in Hokkaido were selected, the first cutting grasses were continuously collected from the same pastures on different harvest days over 4 years (2011-2014), and changes in the water soluble carbohydrates (WSC) content were analyzed to investigate the relationship with weather conditions. The harvest days in the 4-year period were June 17-22, June 24-26, June 27-30, and July 2-5, and grasses on these dates were designated as the harvest date groups. The weather data during the cutting period in each year were utilized. The WSC content tended to change yearly and it was significantly lower in the 4 groups (67.3 - 82.1 g/kg DM) in 2011 than in the groups (101.5 - 130.3 g/kg DM) in 2013 excluding the June 24-26 group (P < 0.05, P < 0.01). The WSC content tended to increase as the harvest date progressed in 2011-2013 and it significantly increased even though the harvest date was delayed only in 2013 (P < 0.01). It was suggested that differences in the WSC content among the years were related to the weather condition and dry matter yield of the grass.

关键词： Grass Harvest Dates Hokkaido Timothy TMR Center Water Soluble Carbohydrates

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NOV对不同突变型戈谢病人β-Glc活性及其底物降解影响

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青岛大学医学院学报 2011年第6期47卷 477-478,481页

作者：侯琳 OHNO kousaku 青岛大学医学院生物化学教研室山东青岛266021 日本鸟取大学医学部神经生物教室

目的观察化学分子伴侣N-Octyl-β-valienamine(NOV)对不同突变型戈谢病人β-葡萄糖脑苷脂酶(β-Glc)活性及其底物降解的影响。方法以化学分子伴侣NOV处理体外培养的不同突变型戈谢病人皮肤成纤维细胞,采用荧光酶学手段测定β-Glc活性;... 详细信息

目的观察化学分子伴侣N-Octyl-β-valienamine(NOV)对不同突变型戈谢病人β-葡萄糖脑苷脂酶(β-Glc)活性及其底物降解的影响。方法以化学分子伴侣NOV处理体外培养的不同突变型戈谢病人皮肤成纤维细胞,采用荧光酶学手段测定β-Glc活性;放射性核素掺入薄层层析法分析该酶底物葡萄糖脑苷脂的降解情况。结果化学分子伴侣NOV可以使体外培养的F213I突变型戈谢病人成纤维细胞内β-Glc活性提高5.7倍;使该突变型细胞内β-Glc的底物葡萄糖脑苷脂的水平下降。结论 NOV可以提高F213I突变型戈谢病人成纤维细胞内β-Glc的活性,加速底物葡萄糖脑苷脂的降解,此可能为F213I突变型戈谢病人提供新的治疗方案。

关键词：戈谢病分子伴侣葡糖苷酰鞘氨醇酶

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Effects of Potassium Fertilizer on Water-Soluble Carbohydrate Content of Timothy (<i>Phleum pratense</i>L.), Silage Fermentation, Nutritive Values, and Nutrient Intake

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American Journal of Plant Sciences 2014年第7期5卷 1030-1038页

作者： Peng Wang kousaku Souma Hideki Okamoto Satomi Kin Akane Sugita Akihiro Furudate Chihiro Sato Akio Nibe Yimin Cai Takayoshi Masuko College of Animal Sciences Jilin University Changchun China Faculty of Bio-Business Management and Information Tokyo University of Agriculture Tokyo Japan Faculty of Bioindustry Tokyo University of Agriculture Hokkaido Japan Japan International Research Center for Agricultural Science Ibaraki Japan Pioneer Hi-Bred Japan Co. Ltd. Hokkaido Branch Hokkaido Japan Tenpoku Branch Hokkaido Prefectural Kamikawa Agricultural Experiment Station (Currently Tenpoku Sub Station Kamikawa Agricultural Experiment Station Hokkaido Research Organization) Hokkaido Japan Central Agricultural Experiment Station Hokkaido Research Organi-zation (Currently) Hokkaido Japan Tenpoku Branch Hokkaido Prefectural Kamikawa Agricultural Experiment Station (Currently Tenpoku Sub Station Kamikawa Agricultural Experiment Station Hokkaido Research Organization) Hokkaido Japan

The objective of this study was to examine the effects of potassium fertilizer (standard (S) and high (H) levels) on water-soluble carbohydrate (WSC) content of timothy (Phleum pratense L.), silage fermentation, nutritive values, and nutrient intake. The silage treatments were as follows: S level without inoculant (SC), S level plus inoculant (SI), H level without inoculant (HC), and H level plus inoculant (HI). The K content was increased by 14.5% in timothy grown with the H level compared with the S level. The WSC contents of the S and H treatments were 75.9 and 66.1 g·kg-1 dry matter (DM), respectively. The silage fermentation quality was low with both SC and HC treatments. The addition of inoculant significantly improved the fermentation quality in SI and HI treatments. The addition increased the DM and organic matter digestibilities of silage. The total digestible nutrient (TDN) content of silage was highest with the HI treatment. The DM, TDN, and digestible energy intakes with the SI and HI treatments were improved compared with the SC and HC treatments, respectively. This study demonstrated negligible effects of K fertilizer levels and significant effects of the lactic acid bacteria inoculant on the fermentation quality, nutritive values, and feed intake of silage.

关键词： Feed Intake Fermentation Quality Nutritive Value Potassium Fertilizer Timothy Silage

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Effects of Addition of Lactobacillus plantarum and Enterococcus faecium Inoculants to High-Nitrogen Fertilized Timothy (Phleum pratense L.) on Fermentation, Nutritive Value, and Feed Intake of Silage

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American Journal of Plant Sciences 2014年第26期5卷 3889-3897页

作者： Peng Wang kousaku Souma Hideki Okamoto Tatsunori Yano Masayuki Nakano Akihiro Furudate Chihiro Sato Jiabao Zhang Takayoshi Masuko College of Animal Science Jilin University Changchun China Faculty of Bioindustry Tokyo University of Agriculture Hokkaido Japan Tenpoku SubStation Kamikawa Agricultural Experiment Station Hokkaido Research Organization Hokkaido Japan Central Agricultural Experiment Station Hokkaido Research Organization Hokkaido Japan Pioneer Hi-Bred Japan Co. Ltd. Hokkaido Branch Hokkaido Japan

The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.

关键词： DCP Intake Fermentation High-Nitrogen Fertilizer Rate Lactobacillus plantarum Timothy Silage

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