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Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice

World Journal of Stem Cells 2023年第4期15卷 268-280页

作者： Takayasu Ohtake Shoichi Itaba Amankeldi A Salybekov Yin Sheng Tsutomu Sato Mitsuru Yanai makoto Imagawa Shigeo Fujii Hiroki Kumagai Masamitsu Harata Takayuki Asahara Shuzo kobayashi Regenerative Medicine The Center for Cell Therapy&Regenerative MedicineShonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Kidney Disease and Transplant center Shonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Regenerative Medicine Shonan Research Institute of Innovative MedicineKamakura 247-8533KanagawaJapan Kamakura Techno-science Inc. Kamakura 248-0036Japan Advanced Medicine Science Tokai University School of MedicineIsehara 259-1193Japan Department of Pathology Sapporo Tokushukai HospitalSapporo 004-0041Japan Department of Pathology Sapporo Medical CenterSapporo 004-0041Japan Human Life CORD Japan Inc Chuo-ku 103-0012TokyoJapan Cell Processing and Cell/Genome Analysis Center The Center for Cell Therapy&Regenerative MedicineShonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Kidney Disease and Transplant Center Shonan Kamakura General HospitalKamakura 247-8533KanazawaJapan

BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.

关键词： Chronic kidney disease CD34+cell Adenine Tubulointerstitial injury Quality and quantity control culture Umbilical cord blood

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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

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Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira kobayashi Masahiko Fukumoto Takato Nomoto Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

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Efficient high harmonics generation by enhancement cavity driven with a post-compressed FCPA laser at 10 MHz

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High Power Laser Science and Engineering 2018年第2期6卷 45-50页

作者： Zhigang Zhao Akira Ozawa makoto Kuwata-Gonokami Yohei kobayashi The Institute for Solid State Physics The University of Tokyo Department of Physics Graduate School of Science The University of Tokyo

Efficient high harmonics generation(HHG) was demonstrated at 10 MHz repetition rate with an external femtosecond enhancement cavity, seeded by a ～70 fs post-compressed 10 MHz fiber chirped pulse amplifier(FCPA) laser. Operation lasting over 30 min with 0.1 m W outcoupled power at 149 nm was demonstrated. It was found that shorter pulse was beneficial for alleviating the nonlinear plasma effect and improving the efficiency of HHG. Low finesse cavity can relax the plasma nonlinearity clamped intra-cavity power and improve the cavity-locking stability. The pulse duration is expected to be below 100 fs for both 1040 nm and 149 nm outputs, making it ideal for applications such as time-resolved photoemission spectroscopy.

关键词： enhancement cavity high harmonics generation ultrafast fiber lasers

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Comprehensive review on small common bile duct stones

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World Journal of Gastroenterology 2023年第13期29卷 1955-1968页

作者： Sakue Masuda Kazuya Koizumi Kento Shionoya Ryuhei Jinushi Makomo Makazu Takashi Nishino Karen Kimura Chihiro Sumida Jun Kubota Chikamasa Ichita Akiko Sasaki Masahiro kobayashi makoto Kako Uojima Haruki Department of Gastroenterology Shonan Kamakura General HospitalKanagawa 247-8533Japan Department of Gastroenterology Kitasato University School of MedicineKanagawa 252-0375Japan

Common bile duct stones are among the most common conditions encountered by endoscopists.Therefore,it is well researched;however,some items,such as indications for endoscopic papillary balloon dilatation(EPBD),safety of EPBD and endoscopic sphincterotomy in patients receiving dual antiplatelet therapy or direct oral anticoagulant,selection strategy for retrieval balloons and baskets,lack adequate evidence.Therefore,the guidelines have been updated with new research,while others remain unchanged due to weak evidence.In this review,we comprehensively summarize the standard methods in guidelines and new findings from recent studies on papillary dilation,stone retrieval devices,difficult-to-treat cases,troubleshooting during the procedure,and complicated cases of cholangitis,cholecystolithiasis,or distal biliary stricture.

关键词： Choledocholithotomy Choledocholithiasis Common bile duct stones Endoscopic papillary balloon dilation Endoscopic sphincterotomy Small common bile duct stones

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Comprehensive review on endoscopic ultrasound-guided tissue acquisition techniques for solid pancreatic tumor

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World Journal of Gastroenterology 2023年第12期29卷 1863-1874页

作者： Sakue Masuda Kazuya Koizumi Kento Shionoya Ryuhei Jinushi Makomo Makazu Takashi Nishino Karen Kimura Chihiro Sumida Jun Kubota Chikamasa Ichita Akiko Sasaki Masahiro kobayashi makoto Kako Uojima Haruki Department of Gastroenterology Shonan Kamakura General HospitalKanagawa 247-8533Japan Department of Gastroenterology Internal MedicineKitasato University School of MedicineKanagawa 252-0375Japan

Pancreatic ductal adenocarcinoma is speculated to become the second leading cause of cancer-related mortality by 2030,a high mortality rate considering the number of cases.Surgery and chemotherapy are the main treatment options,but they are burdensome for patients.A clear histological diagnosis is needed to determine a treatment plan,and endoscopic ultrasound(EUS)-guided tissue acquisition(TA)is a suitable technique that does not worsen the cancer-specific prognosis even for lesions at risk of needle tract seeding.With the development of personalized medicine and precision treatment,there has been an increasing demand to increase cell counts and collect specimens while preserving tissue structure,leading to the development of the fine-needle biopsy(FNB)needle.EUS-FNB is rapidly replacing EUS-guided fine-needle aspiration(FNA)as the procedure of choice for EUS-TA of pancreatic cancer.However,EUS-FNA is sometimes necessary where the FNB needle cannot penetrate small hard lesions,so it is important clinicians are familiar with both.Given these recent developments,we present an up-to-date review of the role of EUS-TA in pancreatic cancer.Particularly,technical aspects,such as needle caliber,negative pressure,and puncture methods,for obtaining an adequate specimen in EUS-TA are discussed.

关键词： Endoscopic ultrasound-guided fine needle biopsy Endoscopic ultrasoundguided tissue acquisition Personalized medicine Genomic profiling test Pancreatic cancer Puncture procedure

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In Vivo Development of Fetal Pig Kidneys in Mature Monkeys under Clinically Approved Immunosuppressant Drugs

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Engineering 2022年第3期8卷 65-73页

作者： Tsuyoshi Takamura Kenji Matsui Naoto Matsumoto Yatsumu Saito Toshinari Fujimoto Susumu Tajiri Shuichiro Yamanaka Kei Matsumoto Akimitsu kobayashi Izumi Yamamoto Hiroshi Sasaki Haruyuki Hirayama Hitomi Matsunari Kazuaki Nakano Hiroshi Nagashima Akihiko Kiyoshi Takao Kuroda makoto Inoue Takeshi Miyawaki Takashi Yokoo Eiji kobayashi Division of Nephrology and Hypertension Department of Internal MedicineThe Jikei University School of MedicineTokyo 105-8461Japan Department of Urology The Jikei University School of MedicineTokyo 105-8461Japan Department of Plastic and Reconstructive Surgery The Jikei University School of MedicineTokyo 105-8461Japan Meiji University International Institute for Bio-Resource Research Kanagawa 214-8571Japan Sumitomo Dainippon Pharma Co. Ltd.Osaka-fu 564-0053Japan Department of Kidney Regenerative Medicine The Jikei University School of MedicineTokyo 105-8461Japan

Controlling the immune response with only clinically approved immunosuppressant drugs is difficult in renal heterotra ns plantation from pigs to nonhuman primates.Moreover,to the best of our knowledge,no reports exist on the use of fetal pigs as kidney donors.This study aimed to compare the degree of transplant rejection between neonatal and fetal kidneys,with genetically unmodified pigs as donors and cynomolgus monkeys as recipients.The left kidneys of the recipient monkeys were removed,followed by transplantation of neonatal as well as fetal pig kidneys,which had undergone vascular anastomosis at the same site,into the retroperitoneum.Immunosuppression was performed with only US Food and Drug Administration-approved drugs.The fetal kidneys were transplanted into the omentum and paraaortic regions of cynomolgus monkeys.Consequently,the engraftment and development of the transplanted tissues were pathologically examined by sampling over time(twice in each experiment).An acute rejection was observed after a few weeks in neonatal renal grafts with vascular anastomosis.However,fetal pig kidneys were spared from rejection despite the administration of the same immunosuppressive protocol to the monkeys and the recipient blood vessels flowing into the fetal kidneys.The immunogenicity of fetal kidneys in pig-monkey renal heterotransplantation was lower than that of neonatal kidneys.

关键词： Cynomolgus monkey Pig Kidney Fetal kidney Immunosuppression

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Cowpea NAC1/NAC2 transcription factors improve growth and tolerance to drought and heat in transgenic cowpea through combined activation of photosynthetic and antioxidant mechanisms

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Journal of Integrative Plant Biology 2023年第1期65卷 25-44页

作者： Richa Srivastava Yuriko kobayashi Hiroyuki Koyama Lingaraj Sahoo Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati Assam 781039 India Faculty of Applied Biological Sciences Gifu University 1-1 YanagidoGifu 501-1193Japan

NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in plants.However,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC orthologs inapt for pulse engineering.The knowledge of suitable NAC candidates in hardy pulses like cowpea(Vigna unguiculata(L.)Walp.)is still in infancy,hence warrants immediate biotechnological intervention.Here,we showed that overexpression of two native NAC genes(VuNAC1and VuNAC2)promoted germinative,vegetative,and reproductive growth and conferred multiple abiotic stress tolerance in a commercial cowpea variety.The transgenic lines displayed increased leaf area,thicker stem,nodule-rich denser root system,early flowering,higher pod production(~3.2-fold and~2.1-fold),and greater seed weight(10.3%and 6.0%).In contrast,transient suppression of VuNAC1/2 caused severe growth retardation and flower inhibition.The overexpressor lines showed remarkable tolerance to major yielddeclining terminal stresses,such as drought,salinity,heat,and cold,and recovered growth and seed production by boosting photosynthetic activity,water use efficiency,membrane integrity,Na^(+)/K^(+)homeostasis,and antioxidant activity.The comparative transcriptome study indicated consolidated activation of genes involved in chloroplast development,photosynthetic complexes,cell division and expansion,cell wall biogenesis,nutrient uptake and metabolism,stress response,abscisic acid,and auxin signaling.Unlike their orthologs,VuNAC1/2 direct synergistic transcriptional tuning of stress and developmental signaling to avoid unwanted trade-offs.Their overexpression governs the favorable interplay of photosynthesis and reactive oxygen species regulation to improve stress recovery,nutritional sufficiency,biomass,and production.This unconventional balance of strong stress tolerance and agronomic quality is useful for translational crop research and molecular breeding of pulses.

关键词： cowpea enhanced photosynthetic activity improved growth improved yield legume genetic engineering multiple stress tolerance NAC transcription factor VuNAC

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Pneumonia and seizures due to hypereosinophilic syndrome—organ damage and eosinophilia without synchronisation:A case report

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World Journal of Clinical Cases 2022年第18期10卷 6325-6332页

作者： Tetsuro Ishida Tomonori Murayama Seiju kobayashi Department of Psychiatry Hokujinkai Ishibashi HospitalOtaru 047-8585Japan Department of Psychiatry Kushiro Red Cross HospitalKushiro 085-8512Japan Department of Psychiatry Shinyukai Nakae HospitalSapporo 001-0022Japan

BACKGROUND Hypereosinophilic syndrome(HES)is a condition characterized by increased eosinophil proliferation in the bone marrow,as well as tissue eosinophilia,often causing organ damage.The cause of the disease is unknown.Initial symptoms include fatigue,cough,shortness of breath,myalgia,angioedema,fever,and pneumonia.In addition to the respiratory symptoms,damage to the central nervous system can lead to severe seizures.Here,we report a case with pneumonia and complex partial seizures secondary to HES.CASE SUMMARY A 94-year-old woman was admitted to our hospital for heart failure and bloody stools.After admission,she also showed symptoms of pneumonia.Non-contrast computed tomography of the chest showed pleural effusion and infiltrative shadows.Lower gastrointestinal endoscopy showed multiple ulcers in the sigmoid colon.Blood analyses showed marked eosinophilia(eosinophils 1760/mm3,total leukocytes 6850/mm3).Initial treatment with furosemide 20 mg/d and prednisolone 25 mg/d relieved these symptoms.However,the patient subsequently experienced localised epileptic seizures characterized by bilateral eyelid twitching and eyes rolling upwards,without generalized convulsions,and respiratory arrest occurred.Electroencephalography showed spikes and waves.Non-contrast magnetic resonance imaging of the brain showed extensive periventricular hyperintensity.With administration of levetiracetam 1000 mg/d the epileptic seizures disappeared.However,the patient’s consciousness remained impaired,and her pneumonia worsened again.Two weeks later,she died of pneumonia.CONCLUSION HES symptoms are variable and atypical,and the level and timing of eosinophilia and organ damage are often discordant.

关键词： Case report Hypereosinophilic syndrome Pneumonia Seizures Prednisolone Levetiracetam

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Stem cell-derived embryo models:a frontier of human embryology

Medical Review

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Medical Review 2023年第4期3卷 343-346页

作者： Norio kobayashi Jianping Fu Department of Mechanical Engineering University of MichiganAnn ArborMIUSA Department of Mechanical Engineering University of MichiganAnn ArborMI 48109USA Department of Cell and Developmental Biology University of Michigan Medical SchoolAnn ArborMI 48109USA Department of Biomedical Engineering University of MichiganAnn ArborMI 48109USA

Studying human development remains difficult due to limited accessibility to human embryonic tissues.Prompted by the availability of human stem cells that share molecular and cellular similarities with embryonic and extraembryonic cells in peri-implantation human embryos,researchers have now successfully developed stem cellbased human embryo models that are promising as experimental tools for studying early human development.In this Perspective,we discuss the current progress in mouse and human stem cell-derived embryo models and highlight their promising applications in advancing the fundamental understanding of mammalian development.

关键词： embryo models embryonic development pluripotent stem cells

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Current research of idiopathic normal pressure hydrocephalus:Pathogenesis,diagnosis and treatment

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World Journal of Clinical Cases 2023年第16期11卷 3706-3713页

作者： Tetsuro Ishida Tomonori Murayama Seiju kobayashi Department of Psychiatry Japan Health Care CollegeSapporo 062-0053HokkaidoJapan Department of Psychiatry Asahikawa Keisenkai HospitalAsahikawa 078-8208HokkaidoJapan Department of Psychiatry Shinyukai Nakae HospitalSapporo 001-0022HokkaidoJapan

Idiopathic normal pressure hydrocephalus(iNPH)is caused by impaired cerebrospinal fluid absorption in the elderly;it is a surgically treatable form of dementia.Gait disturbance,dementia,and urinary incontinence are the triad of signs for iNPH.In addition to these clinical findings,imaging studies show characteristic ventricular enlargement.High Evans Index and‘disproportionately enlarged subarachnoid hydrocephalus’are other well-known imaging findings of iNPH.If the tap test shows improved symptoms,shunt surgery is performed.The disease was first described by Hakim and Adams in 1965,followed by the publication of the first,second,and third editions of the guidelines in 2004,2012,and 2020,respectively.Recent studies signal the glymphatic system and classical cerebrospinal fluid(CSF)absorption from the dural lymphatics as aetiological mechanisms of CSF retention.Research is also underway on imaging test and biomarker developments for more precise diagnosis,shunting technique options with fewer sequelae and complications,and the influence of genetics.Particularly,the newly introduced‘suspected iNPH’in the third edition of the guidelines may be useful for earlier diagnosis.However,less well-studied areas remain,such as pharmacotherapy in non-operative indications and neurological findings other than the triadic signs.This review briefly presents previous research on these and future issues.

关键词： Review Idiopathic normal pressure hydrocephalus Treatable dementia Shunt surgery Drug therapy

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