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Effect of estrogen on manganese-induced toxicity on embryonic astrocytes

Advances in Bioscience and Biotechnology 2013年第10期4卷 9-15页

作者： Tyler T. Huynh kimberly j. Baker Harold L. Komiskey Department of Biomedical Sciences Philadelphia College of Osteopathic Medicine-GA Campus Suwanee USA

Manganese (Mn) is a natural trace metal that is essential for many physiological functions in the human body. Astrocytes in the central nervous system are susceptible reservoirs for Mn accumulation. Estrogen, a steroidal hormone, has been shown to mitigate Mn-induced toxicity in cultures of postnatal astrocytes. However, differences in expression/inducibility of glutamate transporters and glutamine synthetase, transmitters, and the natural gonadal steroids and their receptors are known to occur in astrocyte cultures derived from various stages of fetal and postnatal development. Cultures of embryonic (E18) hippocampal astrocytes were examined in this study for the ability of 17 β-estradiol (E2) to protect them from Mn toxicity by up regulating gene expression of a glutamate transporter. Primary rat hippocampal astrocytes were pretreated with β-Estradiol (E2) in vitro and subsequently, Mn sulfate (MnSO4). The amount of toxic damage to the astrocytes was measured by quantifying glial fibrillary acidic protein (GFAP) with a sandwiched Enzyme-Linked Immunosorbent Assay (ELISA). ELISA analysis indicated Mn exposure at 100 μM, 300 μM, or 600 μM significantly increased GFAP levels. However, E2 concentrations at 10 nM or 30 nM significantly reduced Mn-induced GFAP concentrations at 100 μM. Cells pretreated with 10 nM or 30 nM of E2 significantly lowered GFAP levels. The Water-Soluble Tetrazolium-8 (WST-8) method was utilized to determine cell viability. The WST-8 assay showed that Mn concentrations of 100 μM, 300 μM, or 600 μM significantly reduced the dehydrogenase activity, thereby decreasing the number of viable astrocytes. Enzyme activity with 600 μM of Mn was significantly decreased when compared with 100 μM of Mn, revealing a dose-dependent effect. However, the dehydrogenase activity in cells treated with 600 μM Mn was significantly increased when pretreated with 10 nM of E2. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) was used to measure changes in gluta

关键词： Astrocytes Estrogen Embryonic Manganese Hippocampus

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Genetics-inspired data-driven approaches explain and predict crop performance fluctuations attributed to changing climatic conditions

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Molecular Plant 2022年第2期15卷 203-206页

作者： Xianran Li Tingting Guo Guihua Bai Zhiwu Zhang Deven See juliet Marshall kimberly A.Garland-Campbell jianming Yu USDA Agricultural Research ServiceWheat HealthGeneticsand Quality Research UnitPullmanWA 99164USA Department of Agronomy Iowa State UniversityAmesIA 50011USA USDA Agricultural Research ServiceHard Winter Wheat Genetics Research UnitManhattanKS 66506USA Department of Crop and Soil Sciences Washington State UniversityPullmanWA 99164USA Department of Plant Sciences University of Idaho Research and ExtensionIdaho FallsID 83402USA

Dear Editor,Genetics-focused approaches have been widely used to uncover major genetic variants associated with performance ***,manipulating,and editing genetic variants significantly improve crop ***,the genetic component explains a portion of performance variation,and the environ-mental component contributes to the remaining,often large,portion(Laidig et al.,2017;Bonecke et al.,2020;Li et al.,2021).To ensure superior and robust performance,elite varieties are extensively tested across multiple years and *** extensive performance records,coupled with climatic profiles,could be leveraged to understand climate's impact on agriculture through approaches parallel to quantitative genetics approaches(Figure 1A).

关键词： performance attributed Figure

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Oncogene addiction and non-oncogene addiction in glioblastoma therapy

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Chinese Medical journal 2011年第17期124卷 2565-2568页

作者： kimberly Ng Clark C. Chen Department of Radiation Oncology Dana-Farber Cancer Institute Boston MA USA Division of Neurosurgery Beth Israel Deaconess Medical Center Boston MA USA

INTRODUCTIONGlioblastoma is the most common form of primary brain tumor. The incidence of this tumor is fairly low, with 2-3 cases per 100 000 people in Europe and North America.

关键词： Oncogene addiction glioblastoma

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Associations between extracurricular activity participation and health-related variables in underrepresented children

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Sports Medicine and Health Science 2020年第2期2卷 102-108页

作者： jeanette M.Ricci kimberly A.Clevenger Sandra Sellers Sharon Davenport Karin A.Pfeiffer Michigan State University 308 W Circle DrEast LansingMI48823USA Crim Fitness Foundation 452 S.Saginaw StSuite 1FlintMI48502USA

Background:Children from underrepresented populations exhibit low levels of physical activity(PA),diet quality,and health-related quality of life(QoL),but participation in extracurricular activities may positively impact these health ***:To examine differences in PA,dietary behavior,and QoL in underrepresented children by extracurricular activity dose(0,1,≥2)and type(sports,dance/martial arts,art/music).Methods:Children(N=754;Grades 4–6)completed the Physical Activity Questionnaire for Children,KidsScreen-27(QoL),and the School Physical Activity and Nutrition Survey and self-reported extracurricular activity ***-way MANOVA tests were used in this ***:Individuals participating in 1 and≥2 activities(p<0.001),sport/dance/martial arts(p<0.001),and art/music(p=0.029)had higher PA than *** participating in≥2 activities reported higher fruit intake compared to 1 activity and non-participants(p=0.009;p<0.001,respectively).Participants with≥2 activities reported higher parent-and peer-related QoL compared to non-participants(p=0.001;p=0.025,respectively).Conclusions:Extracurricular activity participation was positively associated with health behaviors in underrepresented *** may be encouraged to allocate some of their resources to extracurricular activity programing.

关键词： Physical activity Dietary behavior Quality of life Youth Sport participation

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Improving bermudagrass in the Southeastern United States with alfalfa as an alternative nitrogen source in grazing systems

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Grassland Research 2022年第4期1卷 280-289页

作者： justin C.Burt Lisa L.Baxter Christopher G.Prevatt Mary kimberly Mullenix R.Lawton Stewart jr. jennifer j.Tucker Department of Animal and Dairy Science University of GeorgiaTiftonGeorgiaUSA Department of Crop and Soil Science University of GeorgiaTiftonGeorgiaUSA Regional Specialized Agent Range Cattle RECUniversity of FloridaGainesvilleFloridaUSA Department of Animal Science Auburn UniversityAuburnAuburnUSA Department of Animal and Dairy Science University of GeorgiaAthensGeorgiaUSA

Background Bermudagrass(Cynodon spp.)is one of the most common perennial warm-season forages grown in the Southeastern United *** incorporation of alfalfa(Medicago sativa)into bermudagrass pastures could serve as an alternative source of nitrogen(N)while also improving the nutritive value of the forage *** A 2-year grazing evaluation arranged in a randomized complete block design was conducted from May to September 2018(Year 1)and May–August 2019(Year 2)in Tifton,GA,*** objective was to compare the production of alfalfa–bermudagrass mixed pastures(BGA)with bermudagrass monoculture pastures with(BGN)or without(BG)the application of synthetic nitrogen on forage production and stocker cattle *** Differences were observed for average daily gain(p=0.10),gain per hectare(p=0.02)and stocking rate(p=0.01),in that BGA was greatest compared to BGN and BG,which did not differ from each ***,BGA had increased forage crude protein(p=0.01)and total digestible nutrients(p=0.01)compared to BGN and ***,BGA provided the greatest economic return per hectare compared to BG or *** These data illustrate improved animal performance and forage nutritive value using alfalfa–bermudagrass systems and offer a viable option for producers seeking alternatives to synthetic N sources for bermudagrass pastures in the Southeastern United States.

关键词： alfalfa-bermudagrass mixtures forage management grazing management

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Finite Element Analysis of Osteotomy Treatment for Kienbock’s Disease

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journal of Biomedical Science and Engineering 2016年第10期9卷 161-170页

作者： Guang Yang kimberly A. Partridge Ahmed Abdel-Mohti Alex j. McMullen Hui Shen Department of Hand Surgery China-Japan Union Hospital of Jilin University Changchun China Department of Mechanical Engineering Ohio Northern University Ada OH USA Department of Civil Engineering Ohio Northern University Ada OH USA

Lateral closing wedge osteotomy is a treatment for Kienbock’s disease. It is one of the most frequently used treatment options, which has been reported with relatively good long-term results. However, the results about the treatment are still controversial in some literatures and some key mechanisms are still not clear. The objective of the cur-rent study was to study the biomedical mechanism of the treatment. A finite element model was developed based on the geometry of carpal bones. Various situations in-cluding inclination angle changes by cutting the radial with 0?, 5?, 10? and 15? osteot-omy angles were studied. The effectiveness of the treatment was also studied for the carpal structure with abnormal positions of the lunate bone. The results show that the effectiveness of the stress reduction with the angle depends on many situations such as the initial morphology of the carpal structure.

关键词： Lateral Closing Wedge Osteotomy Kienbock’s Disease Finite Element Biomedical Treatment

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雪地上的森林冠层混合反照率模型

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遥感学报 2003年第6期7卷 433-439页

作者：蒋玲梅杨华王锦地李小文 kimberly j.Schaudt 北京师范大学遥感与GIS研究中心 Arizona Univ.Tucson AZ85721USA

反照率通常定义为出射与入射的能量之比 ,是地表能量平衡中的关键因子。气候变化对反照率也很敏感。典型的气候变化预测是通过GCM与地表模型的结合进行的。目前在地表模型 (如BATS模型 )中 ,是通过空气动力学粗糙度Z0 与降雪深度d两个... 详细信息

反照率通常定义为出射与入射的能量之比 ,是地表能量平衡中的关键因子。气候变化对反照率也很敏感。典型的气候变化预测是通过GCM与地表模型的结合进行的。目前在地表模型 (如BATS模型 )中 ,是通过空气动力学粗糙度Z0 与降雪深度d两个参数来估计雪地上树冠的反照率的。在他们的工作基础上 ,重点考虑对于不同的太阳天顶角 ,直入—扇出反照率的方向性及其与叶面积指数 (LAI)的关系。同时 ,为了保持BATS模型所需反照率模型的基本特征 ,主要在几何光学—辐射传输混合模型 (GORT)的基础上做了一些近似来达到此目的。最后 ,以针叶林为例 ,用MODIS的反照率数据和BOREAS地面观测数据做了初步的模型验证。

关键词：反照率模型森林冠层混合像元反照率方向间隙率开放度 GORT模型

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Oncomorphic <i>TP</i>53 Mutations in Gynecologic Cancers Lose the Normal Protein:Protein Interactions with the microRNA Microprocessing Complex

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journal of Cancer Therapy 2014年第6期5卷 506-516页

作者： Pavla Brachova Samuel R. Mueting Eric j. Devor kimberly K. Leslie Department of Obstetrics and Gynecology University of Iowa Iowa City USA Department of Pediatrics University of Iowa Iowa City USA

Mutations in the tumor suppressor TP53 occur in almost all advanced ovarian cancers and in many advanced serous endometrial cancers. Mutations in TP53 can alter the function of the p53 protein, and some mutations result in a mutated protein with oncogenic activity. Previously referred to as gain of function (GOF) p53 proteins, we now term these “oncomorphic” mutations to better describe their function as oncogenes. We reviewed the data from The Cancer Genome Atlas (TCGA) and demonstrate that of the patients diagnosed with endometrial cancer that harbor TP53 mutations, approximately 30% of these mutations are oncomorphic. In ovarian cancer, approximately 20% are oncomorphic. The wild type (WT) p53 protein transactivates genes and micro-RNAs (miRNAs) necessary in the response to cellular stress, which turn off growth and induce apoptosis. In addition to direct transcriptional activation, WT p53 also acts through protein:protein interactions with Drosha and the miRNA processing complex to mediate rapid, enhanced processing of a subset of anti-growth miRNAs. We validated the interaction of WT p53 with the Drosha complex in the cell line UCI-107. We observed that miRNAs that inhibit the expression of oncogenes were induced. Specifically, some miRNAs were induced very rapidly over minutes, consistent with enhanced processing, while others required hours, consistent with transcriptional activation. In contrast, the most common oncomorphic TP53 mutations failed to interact with the Drosha complex and lost the ability to rapidly induce the miRNAs which inhibit oncogene expression. These studies highlight one mechanism underlying the oncomorphic properties of specific TP53 mutations: loss of the enhanced processing of anti-proliferative miRNAs.

关键词： TP53 p53 miRNA Drosha Oncomorphic TP53 Gain of Function

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飞扬草中的三菇成分及其细胞毒活性(英文)

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中国天然药物 2013年第5期11卷 528-533页

作者： Consolacion Y.Ragasa kimberly B.Cornelio Chemistry Department and Center for Natural Sciences and Ecological Research De La Salle University

AIM:To investigate the chemical constituents of the stems,leaves and roots of Euphorbia hirta,and to test for the cytotoxic and antimicrobial potentials of the major constituents of the ***: The compounds were isolated by silica gel chromatography and their structures were elucidated by NMR *** cytotoxicity tests were conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,while the antimicrobial tests employed the agar well ***: The air-dried stems of *** afforded taraxerone 1,a mixture of 25-hydroperoxycycloart-23-en-3-ol(2a) and 24-hydroperoxycycloart-25-en-3-ol(2b)(sample 2) in a 2:1 ratio,and another mixture of cycloartenol(3a),lupeol(3b),-amyrin(3c) and-amyrin(3d)(sample 3) in a 0.5:4:1:1 *** air-dried leaves of *** yielded sample 2 in a 3:2 ratio,sample 3 in a 2:3:1:1 ratio,phytol and phytyl fatty acid ester,while the roots afforded sample 2 in a 2:1 ratio,sample 3 in a 2:1:1:1 ratio,a mixture of cycloartenyl fatty acid ester 4a,lupeol fatty acid ester 4b,-amyrin fatty acid ester 4c and-amyrin fatty acid ester 4d(sample 4) in a 3:2:1:1 ratio,linoleic acid,-sitosterol and *** 1 from the stems,sample 2 from the leaves,and sample 3 from the stems were assessed for cytotoxicity against a human cancer cell line,colon carcinoma(HCT 116).Sample 2 showed good activity with an IC50 value of 4.8 g·mL 1,while 1 and sample 3 were inactive against HCT *** 2 was further tested for cytotoxicity against non-small cell lung adenocarcinoma(A549).It showed good activity against this cell line with an IC50 value of 4.5 g·mL *** assays were conducted on 1 and sample *** of the study indicated that 1 was active against the bacteria: Pseudomonas aeruginosa and Staphylococcus aureus,but was inactive against Escherichia coli and Bacillus *** 2 was active against the bacteria: Pseudomonas aeruginosa,Staphylococcus aureus and Escherichia coli and fungi:

关键词： Euphorbia hirta Asteraceae 25-Hydroperoxycycloart-23-en-3-ol 24-Hydroperoxycycloart-25-en-3-ol Cytotoxicity

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Improving detection of cerebral small vessel disease aetiology in patients with isolated lobar intracerebral haemorrhage

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Stroke & Vascular Neurology 2023年第1期8卷 26-33页

作者： Alvin S Das Elif Gokcal Robert W Regenhardt Mitchell j Horn Kristin Schwab Nader Daoud Anand Viswanathan W Taylor kimberly joshua N Goldstein Alessandro Biffi Natalia Rost jonathan Rosand Lee H Schwamm Steven M Greenberg M Edip Gurol Department of Neurology Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMassachusettsUSA Department of Neurology Massachusetts General HospitalHarvard Medical SchoolBostonMassachusettsUSA Department of Neurosurgery Massachusetts General HospitalHarvard Medical SchoolBostonMassachusettsUSA Department of Emergency Medicine Massachusetts General HospitalHarvard Medical SchoolBostonMassachusettsUSA

Background and purpose We evaluate whether non-haemorrhagic imaging markers(NHIM)(white matter hyperintensity patterns,lacunes and enlarged perivascular spaces(EPVS))can discriminate cerebral amyloid angiopathy(CAA)from hypertensive cerebral small vessel disease(HTN-cSVD)among patients with isolated lobar intracerebral haemorrhage(isolated-LICH).Methods In patients with isolated-LICH,four cSVD aetiologic groups were created by incorporating the presence/distribution of NHIM:HTN-cSVD pattern,CAA pattern,mixed NHIM and no *** pattern consisted of patients with any combination of severe centrum semiovale EPVS,lobar lacunes or multiple subcortical spots ***-cSVD pattern consisted of any HTN-cSVD markers:severe basal ganglia PVS,deep lacunes or peribasal ganglia white matter hyperintensity *** NHIM consisted of at least one imaging marker from either *** hypothesis was that patients with HTN-cSVD pattern/mixed NHIM would have a higher frequency of left ventricular hypertrophy(LVH),which is associated with *** In 261 patients with isolated-LICH,CAA pattern was diagnosed in 93 patients,HTN-cSVD pattern in 53 patients,mixed NHIM in 19 patients and no NHIM in 96 *** frequency of LVH was similar among those with HTN-cSVD pattern and mixed NHIM(50%vs 39%,p=0.418)but was more frequent in HTN-cSVD pattern compared with CAA pattern(50%vs 20%,p<0.001).In a regression model,HTN-cSVD pattern(OR:7.38;95%CI 2.84 to 19.20)and mixed NHIM(OR:4.45;95%CI 1.25 to 15.90)were found to be independently associated with *** Among patients with isolated-LICH,NHIM may help differentiate HTN-cSVD from CAA,using LVH as a marker for HTN-cSVD.

关键词： patients cerebral hypertensive

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