飞扬草中的三菇成分及其细胞毒活性(英文)

作     者：Consolacion Y.Ragasa Kimberly B.Cornelio 

作者机构：Chemistry Department and Center for Natural Sciences and Ecological ResearchDe La Salle University 

出 版 物：《中国天然药物》 (Chinese Journal of Natural Medicines)

年 卷 期：2013年第11卷第5期

页      面：528-533页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the grant from the De La Salle University Science Foundation 

主　　题：Euphorbia hirta Asteraceae 25-Hydroperoxycycloart-23-en-3-ol 24-Hydroperoxycycloart-25-en-3-ol Cytotoxicity 

摘      要：AIM:To investigate the chemical constituents of the stems,leaves and roots of Euphorbia hirta,and to test for the cytotoxic and antimicrobial potentials of the major constituents of the ***: The compounds were isolated by silica gel chromatography and their structures were elucidated by NMR *** cytotoxicity tests were conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,while the antimicrobial tests employed the agar well ***: The air-dried stems of *** afforded taraxerone 1,a mixture of 25-hydroperoxycycloart-23-en-3-ol(2a) and 24-hydroperoxycycloart-25-en-3-ol(2b)(sample 2) in a 2:1 ratio,and another mixture of cycloartenol(3a),lupeol(3b),-amyrin(3c) and-amyrin(3d)(sample 3) in a 0.5:4:1:1 *** air-dried leaves of *** yielded sample 2 in a 3:2 ratio,sample 3 in a 2:3:1:1 ratio,phytol and phytyl fatty acid ester,while the roots afforded sample 2 in a 2:1 ratio,sample 3 in a 2:1:1:1 ratio,a mixture of cycloartenyl fatty acid ester 4a,lupeol fatty acid ester 4b,-amyrin fatty acid ester 4c and-amyrin fatty acid ester 4d(sample 4) in a 3:2:1:1 ratio,linoleic acid,-sitosterol and *** 1 from the stems,sample 2 from the leaves,and sample 3 from the stems were assessed for cytotoxicity against a human cancer cell line,colon carcinoma(HCT 116).Sample 2 showed good activity with an IC50 value of 4.8 g·mL 1,while 1 and sample 3 were inactive against HCT *** 2 was further tested for cytotoxicity against non-small cell lung adenocarcinoma(A549).It showed good activity against this cell line with an IC50 value of 4.5 g·mL *** assays were conducted on 1 and sample *** of the study indicated that 1 was active against the bacteria: Pseudomonas aeruginosa and Staphylococcus aureus,but was inactive against Escherichia coli and Bacillus *** 2 was active against the bacteria: Pseudomonas aeruginosa,Staphylococcus aureus and Escherichia coli and fungi: