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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun kim Jun-Koo Yi jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Effect of transcranial direct current stimulation on supernumerary phantom limb pain in spinal cord injured patient:A case report

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World Journal of Clinical Cases 2024年第17期12卷 3177-3182页

作者： Hyo-Sik Park jae-Hyung kim Department of Interventional Physiatry Eulji University Medical CenterSeo-GuDaejeonSouth Korea

BACKGROUND Supernumerary phantom limb(SPL)sensation is the experience of additional limbs,either single or a pair of *** to traumatic spinal cord injuries,we report effect of transcranial direct current stimulation(tDCS)on SPL pain in a patient with cervical cord *** SUMMARY The subject was a 57-year-old man who was diagnosed with complete spinal cord injury(C6/C5,motor level;C5/C5,sensory level;AIS-A)approximately three months *** a period of 2 wk,we administered anodal tDCS over the motor cortex for 15 minutes at an intensity of 1.5 *** that treatment,the patient experienced a decrease of SPL pain intensity and frequency,which lasted for 1 week after the end of *** Targeting the motor cortex through neuromodulation appears to be a promising option for the management of SPL pain.

关键词： Supernumerary phantom limb pain Spinal cord injury transcranial Direct Current Stimulation Neuromodulation Case report

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Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles

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Asian Pacific Journal of Tropical Biomedicine 2024年第2期14卷 47-54页

作者： Hoibin Jeong Dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae kim Seung Hyun kim jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park Bohyun Yun Su-Hyeon Cho Kil-Nam kim Seoul Center Korea Basic Science InstituteSeoul 02841Republic of Korea Chuncheon Center Korea Basic Science InstituteChuncheon 24341Republic of Korea Research&Development Center Chungdam CDC JNPharm LLC.Chuncheon 24341Republic of Korea Department of Biomedical Technology Kangwon National UniversityChuncheon 24341Republic of Korea Yonsei Institute of Pharmaceutical Sciences College of PharmacyYonsei UniversityIncheon 21983Republic of Korea Gwangju Center Korea Basic Science InstituteGwangju 61751Republic of Korea Honam National Institute of Biological Resources 99Gohadoan-gilMokpo-siJeollanam-do58762Republic of Korea Department of Bio-analysis Science University of Science&TechnologyDaejeon34113Republic of Korea

Objective:To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in ***:Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** proliferation was assessed using a 5-bromo-2’-deoxyuridine(BrdU)assay *** blot analysis was performed to determine the protein expressions of related *** effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength *** composition analysis was performed using high-performance liquid chromatography(HPLC).Results:Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling *** also induced metabolic changes,increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein *** an in vivo study,the extract-treated mice showed improved motor abilities,such as muscular endurance and grip ***,HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle ***:Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles,suggesting its potential as an effective natural agent for improving muscular strength.

关键词： Catalpa bignonioides Skeletal muscle Cell proliferation Mitochondria Energy metabolism C2C12

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Highly enhanced UV absorption and light emission of monolayer WS_(2)through hybridization with Ti_(2)N MXene quantum dots and g-C_(3)N_(4)quantum dots

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Opto-Electronic Advances 2024年第6期7卷 31-39页

作者： Anir S.Sharbirin Rebekah E.kong Wendy B.Mato Trang Thu Tran Eunji Lee Jolene W.P.Khor Afrizal L.Fadli Jeongyong kim Department of Energy Science Sungkyunkwan UniversitySuwon 16419Republic of Korea

Two-dimensional(2D)transition metal dichalcogenides(TMD)are atomically thin semiconductors with promising optoelectronic applications across the visible ***,their intrinsically weak light absorption and the low photoluminescence quantum yield(PLQY)restrict their performance and potential use,especially in ultraviolet(UV)wavelength light *** dots(QD)derived from 2D materials(2D/QD)provide efficient light absorption and emission of which energy can be tuned for desirable light *** this study,we greatly enhanced the photon absorption and PLQY of monolayer(1L)tungsten disulfide(WS_(2))in the UV range via hybridization with 2D/QD,particularly titanium nitride MXene QD(Ti_(2)N MQD)and graphitic carbon nitride QD(GCNQD).With the hybridization of MQD or GCNQD,1LWS_(2)showed a maximum PL enhancement by 15 times with 300 nm wavelength excitation,while no noticeable enhancement was observed when the excitation photon energy was less than the bandgap of the QD,indicating that UV absorption by the QD played a crucial role in enhancing the light emission of 1L-WS_(2)in our 0D/2D hybrid *** findings present a convenient method for enhancing the photo-response of 1L-WS_(2)to UV light and offer exciting possibilities for harvesting UV energy using 1L-TMD.

关键词： monolayer TMD WS_(2) 2D-derived quantum dots UV absorption energy transfer

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Boosted Lithium-Ion Transport Kinetics in n-Type Siloxene Anodes Enabled by Selective Nucleophilic Substitution of Phosphorus

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Nano-Micro Letters 2024年第10期16卷 618-637页

作者： Se In kim Woong-Ju kim Jin Gu Kang Dong-Wan kim School of Civil Environmental and Architectural EngineeringKorea University02841 SeoulSouth Korea Nanophotonics Research Center Korea Institute of Science and Technology02792 SeoulSouth Korea

Doped two-dimensional(2D)materials hold significant promise for advancing many technologies,such as microelectronics,optoelectronics,and energy ***,n-type 2D oxidized Si nanosheets,namely n-type siloxene(n-SX),are employed as Li-ion battery *** thermal evaporation of sodium hypophosphite at 275℃,P atoms are effectively incorporated into siloxene(SX)without compromising its 2D layered morphology and unique Kautsky-type crystal ***,selective nucleophilic substitution occurs,with only Si atoms being replaced by P atoms in the O_(3)≡Si-H *** resulting n-SX possesses two delocalized electrons arising from the presence of two electron donor types:(i)P atoms residing in Si sites and(ii)H *** doping concentrations are varied by controlling the amount of precursors or their mean free *** at 2000 mA g^(-1),the n-SX electrode with the optimized doping concentration(6.7×10^(19) atoms cm^(-3))delivers a capacity of 594 mAh g^(-1) with a 73%capacity retention after 500 *** improvements originate from the enhanced kinetics of charge transport processes,including electronic conduction,charge transfer,and solid-state *** approach proposed herein offers an unprecedented route for engineering SX anodes to boost Li-ion storage.

关键词： Li-ion battery Two-dimensional N-type siloxene Doping mechanism Kinetics

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Regulation of sleep by astrocytes in the hypothalamic ventrolateral preoptic nucleus

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Neural Regeneration Research 2025年第4期20卷 1098-1100页

作者： jae-Hong kim Ruqayya Afridi Il-Sung Jang Maan Gee Lee Kyoungho Suk Brain Korea 21 four KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents School of MedicineKyungpook National UniversityDaeguRepublic of Korea Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea Department of Pharmacology School of DentistryKyungpook National UniversityDaeguRepublic of Korea Brain Science&Engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Astrocytes are functionally dynamic cells that support neurons in multiple ways throughout an organism’s *** astrocytic regulation of neuronal activity has been increasingly recognized in recent *** are now recognized as playing a more complex role than mere bystanders in the central nervous ***,their role in regulating the sleep neurocircuitry is not well *** this perspective,we highlight the role of astrocytes in sleep modulation,with a particular focus on regulatory mechanisms related to the ventrolateral preoptic nucleus(VLPO)of the *** briefly discuss recent literature reporting the role of VLPO astrocytes in regulating sleep-associated behaviors.

关键词： sleep ventrolateral astrocytes

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Changes in microorganisms and metabolites during long-term fermentation of commercially manufactured anchovy sauce

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Food Science and Human Wellness 2024年

作者： Daehyeon kim Seong-Eun Park Eun-Ju kim Seung-Ho Seo Kwang-Moon Cho Sun jae Kwon Hong-Seok Son Department of Biotechnology， College of Life Sciences and Biotechnology， Korea University Sonlab Inc. 3. AccuGene Inc.

This study was performed to investigate the changes in microbial communities and metabolites during the long-term fermentation of commercially manufactured anchovy sauce. Samples of commercial anchovy sauce were collected from large-scale fermentation tanks with fermentation periods of up to 7.87 years. The complex bacterial community was simplified to two genera， Tetragenococcus and Halanaerobium， after approximately 0.55 years of fermentation. Although genera， such as Saccharomyces， Cladosporium， Candida， and Aspergillus， were relatively dominant， no clear pattern was identified in fungal community analysis. The longitudinal metabolite profile demonstrated that approximately half (55.8%) of the metabolites present in anchovy sauce were produced within a year of fermentation due to rapid fermentation. Despite the static microbial community， the contents of several metabolites including amino acids and biogenic amines changed continuously during the long-term fermentation of anchovy sauce. This study provides novel insights into the changes in microbiota and metabolites in fish sauce produced without any starter inoculation.

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Investigation of operation and degradation mechanisms in ZnTeSe blue quantum-dot light-emitting diodes by identifying recombination zone

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Nano Research 2024年第7期17卷 6527-6533页

作者： Oul Cho Sujin Park Hogeun Chang Jiwhan kim jaekwon kim Sungwoo kim Taehyung kim Jeonghun Kwak Department of Electrical and Computer Engineering Inter-university Semiconductor Research Centerand Soft Foundry InstituteSeoul National UniversitySeoul 08826Republic of Korea Material Research Center Samsung Advanced Institute of TechnologySuwonGyeonggi-do 16419Republic of Korea

ZnTeSe quantum dots(QDs),recognized as promising eco-friendly blue electroluminescent emitters,remain under-explored in light-emitting diode(LED)***,to elucidate the operation and degradation mechanisms of ZnTeSe blue QD-LEDs,stacked ZnTeSe QD layers with discernable luminescence are designed by varying Te doping concentrations,and the recombination zones(RZs)of the blue QD-LEDs are *** RZs are identified near the hole-transport layer(HTL),confirmed by angular-dependent electroluminescence measurements and optical *** addition,in order to investigate carrier dynamics in the process of recombination,the transient electroluminescence(tr-EL)signals of the dichromatic QD-LEDs are *** a result,it is inferred that the RZ initially formed near the electron-transport layer(ETL)due to the high injection barriers of ***,due to the fast electron mobility,the RZ shifts toward the HTL as the operating current *** the device lifetime tests,the RZ remains stationary while the photoluminescence(PL)corresponding to the RZ undergoes a substantial decrease,indicating that the degradation is accelerated by the concentrated *** this study contributes to a deeper understanding of the operational mechanisms of ZnTeSe blue QD-LEDs.

关键词： quantum dots light-emitting diodes degradation recombination zones carrier dynamics

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Dragonfly Interaction Algorithm for Optimization of Queuing Delay in Industrial Wireless Networks

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Journal of Bionic Engineering 2024年第1期21卷 447-485页

作者： Sanjay Bhardwaj Da-Hye kim Dong-Seong kim Department of IT Convergence Engineering ICT Convergence Research CenterKumoh National Institute of TechnologyGumiSouth Korea

In industrial wireless networks,data transmitted from source to destination are highly *** often leads to the queuing of the data,and poor management of the queued data results in excessive delays,increased energy consumption,and packet ***,a nature-inspired-based Dragonfly Interaction Optimization Algorithm(DMOA)is proposed for optimization of the queue delay in industrial wireless *** term“interaction”herein used is the characterization of the“flying movement”of the dragonfly towards damselflies(female dragonflies)for *** a result,interaction is represented as the flow of transmitted data packets,or traffic,from the source to the base *** includes each and every feature of dragonfly movement as well as awareness of the rival dragonflies,predators,and damselflies for the desired optimization of the queue *** features are juxtaposed as noise and interference,which are further used in the calculation of industrial wireless metrics:latency,error rate(reliability),throughput,energy efficiency,and fairness for the optimization of the queue *** analysis,convergence analysis,the Wilcoxon test,the Friedman test,and the classical as well as the 2014 IEEE Congress of Evolutionary Computation(CEC)on the benchmark functions are also used for the evaluation of DMOA in terms of its robustness and *** results demonstrate the robustness of the proposed algorithm for both classical and benchmarking functions of the IEEE CEC ***,the accuracy and efficacy of DMOA were demonstrated by means of the convergence rate,Wilcoxon testing,and ***,fairness using Jain’s index in queue delay optimization in terms of throughput and latency,along with computational complexity,is also evaluated and compared with other *** results show that DMOA exceeds other bio-inspired optimization algorithms in terms of fairness in queue delay management and average packet *** proposed a

关键词： Dragonfly Damselfly Interaction Queuing delay Optimization Industrial wireless networks

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Ag-GST/HfO_(x)-based unidirectional threshold switching selector with low leakage current and threshold voltage distribution for high-density cross-point arrays

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Rare Metals 2024年第1期43卷 280-288页

作者： Kyoung-Joung Yoo Dae-Yun Kang Nahyun kim Ho-Jin Lee Ta-Hyeong kim Taeho kim Tae Geun kim School of Electrical Engineering Korea UniversitySeoul 02841Republic of Korea

The use of electrochemical-metallization-based volatile threshold switching selectors in cross-point arrays has been widely explored owing to their high on-off ratios and simple ***,these devices are unsuitable for cross-point architectures because of the difficulty in controlling the random filament formation that results in large fluctuations in the threshold voltage during *** this study,we investigated the unidirectional threshold transition characteristics associated with an Ag/GST/HfO_(x)/Pt-based bilayer selector and demonstrated the occurrence of a low leakage current(<1×10^(-11) A) and low distribution of the threshold voltage(Δ0.11 V).The bilayer structure could control the filament formation in the intermediate state through the insertion of an HfO_(x) tunneling *** stacking a bilayer selector with NiO_(x)based resistive random-access memory,the leakage and programming currents of the device could be significantly *** the crossbar array configuration,we performed equivalent circuit analysis of a one-selector oneresistor(1S1R) devices and estimated the optimal array size to demonstrate the applicability of the proposed *** maximum acceptable crossbar array size of the 1S1R device with the Ag/GST/HfO_(x)/Pt/Ti/NiO_(x)/Pt structure was 5.29×10^(14)(N^(2),N=2.3×10^(7)).

关键词： Selector device Tunneling barrier Threshold switching Sputtering 1S1R Cross-point array

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