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Journal of Integrative Plant Biology 2008年第9期50卷 1057-1069页

作者： Yin Li Zinan Wang Xiaofen Sun kexuan tang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Plant Biotechnology Research Center School of Agriculture and Biology Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Shanghai Jiao Tong University Shanghai 200030 China

As a member of an important group of lipid soluble antioxidants, tocopherols play a paramount role in the daily diet of humans and animals. Recently, genes required for tocochromanol biosynthesis pathway have been identified and cloned with the help of genomics-based approaches and molecular manipulation in the model organisms： Arabidopsis thaliana and Synechocystis sp. PCC 6803. At the basis of these foundations, genetic manipulation of tocochromanol biosynthesis pathway can give rise to strategies that enhance the level of tocochromanol content or convert the constitution of tocochromanol. In addition, genetic manipulations of the tocochromanol biosynthesis pathway provide help for the study of the function of tocopherol in plant systems. The present article summarizes recent advances and pays special attention to the functions of tocopherol in plants. The roles of tocopherol in the network of reactiv.e oxygen species, antioxidants and phytohormones to maintain redox homeostasis and the functions of tocopherol as a signal molecule in chloroplast-to- nucleus signaling to regulate carbohydrate metabolism are also discussed.

关键词： antioxidant carbohydrate metabolism genetic dissection programmed cell death redox homeostasis signal molecule tocopherol.

Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

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Science Bulletin 2016年第1期61卷 18-25页

作者： Qian Shen Tingxiang Yan Xueqing Fu kexuan tang Key Laboratory of Urban Agriculture (South) Ministry of Agriculture Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University

Artemisinin, also known as qinghaosu, a sesquiterpene endoperoxide lactone isolated from the Chinese medicinal plant Artemisia annua L., is the most effective antimalarial drug which has saved millions of *** to its great antimalarial activity and low content in wild A. annua plants, researches focused on enhancing the artemisin yield in plants became a hotspot. Several families of transcription factors have been reported to participate in regulating the biosynthesis and accumulation of *** this review, we summarize recent investigations in these fields, with emphasis on newly identified transcription factors and their functions in artemisinin biosynthesis regulation, and provide new insight for further research.

关键词： Artemisia annua L. Artemisinin ,Transcription factor Biosynthesis

Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance in Arabidopsis

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Journal of Integrative Plant Biology 2010年第4期52卷 400-409页

作者： zinan wang Ying Xiao Wansheng Chen kexuan tang Lei Zhang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai 200433 China Department of Pharmacy Changzheng Hospital second Military Medical University Shanghai 200003 China

Vitamin C （L-ascorbic acid, AsA） has important antioxidant and metabolic functions in both plants and animals. Once used, ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase （DHAR, EC 1.***.5.1）. To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress. A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana. Transgenic plants were biochemically characterized and tested for responses to oxidative stress. Western blot indicated that the dhar-transgene was successfully expressed. In homozygous T4 transgenic seedlings, DHAR overexpression was increased up to 1.5 to 5.4 fold, which enhanced foliar ascorbic acid levels 2- to 4.25-fold and ratio of AsA/DHA about 3- to 16-fold relative to wild type. In addition, the level of glutathione, the reductant used by DHAR, also increased as did its redox state. When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10μM paraquat, transgenic plants showed a larger AsA pool size, lower membrane damage, and a higher level of chlorophyll compared with controls. These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress.

关键词： Stress Tolerance Increased Vitamin Enhanced Recycling

Diversity and versatile functions of metallothioneins produced by plants:A review

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Pedosphere 2020年第5期30卷 577-588页

作者： SAEED-UR-RAHMAN Muhammad KHALD Nan HUI Sadaf-Ilyas KAYANI kexuan tang Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)o f Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240(China) Key Laboratory of Urban Agriculture School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240(China)

Metal ions are essential for plant growth and development,but in excess,these compounds can become highly *** have adopted numerous ways to maintain metal homeostasis while mitigating adverse effects of excess metal ions,including phytochelatin and the metal-chelating proteins metallothioneins(MTs).A family of cysteine(Cys)-rich,intracellular,and low-molecular-weight(4-8 kDa)MTs are proteins found in nearly all phyla including plants,animals,and fungi,and they have the potential to scavenge reactive oxygen species and detoxify toxic metals including copper,cadmium,and *** on their Cys numbers and residues,MTs have been categorized into three major *** I MTs,which have highly conserved Cys residues,are found in animals,while class II MTs,with less conserved Cys residues,are present in plants and are classified further into four *** III MTs include phytochelatins,a group of enzymatically synthesized Cys-rich *** MTs have been an area of interest for five decades with extensive studies,which have been facilitated by advancements in instrumental techniques,protein science,and molecular biology ***,we reviewed current advances in our understanding of the regulation of MT biosynthesis,their expression,and their potential roles in the alleviation of abiotic stresses(i.e.,drought,salinity,and oxidative stresses)and heavy metal detoxification and homeostasis.

关键词： abiotic stress biosynthesis gene expression heavy metals reactive oxygen species enzyme regulation

Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper

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Chinese Science Bulletin 2001年第20期46卷 1698-1703页

作者： SUN Xiaofen tang kexuan WAN Bingliang Ql Huaxiong LU Xinggui State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life SciencesFudan UniversityShanghai 200433China Hybrid Rice Research Center Hubei Academy of Agricultural SciencesWuhan 430064China

Mature seed-derived callo from two elite Chinese japonica rice (Oryza saliva L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromy-cin phosphotransferase gene (hpt), the reporter β-glu-curonidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns, we identified five independent homozygous lines containing and expressing all the three transgenes. Insect

关键词： Oryza saliva L. gna transgenic rice bioassay feeding test.

Progress in NMR-based metabolomics of Catharanthus roseus

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Frontiers of Agricultural Science and Engineering 2015年第3期2卷 195-204页

作者： Qifang PAN Jingya ZHAO Yuliang WANG kexuan tang Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Metabolomics has been rapidly developed as an important field in plant sciences and natural products *** the only natural source for a diversity of monoterpenoid indole alkaloids(MIAs),especially the low-abundance antitumor agents vinblastine and vincristine,Catharanthus roseus is highly valued and has been studied extensively as a model for medicinal plants *** to multistep enzymatic biosynthesis and complex regulation,genetic modification in the MIA pathway has resulted in complicated changes of both secondary and primary metabolism in ***,affecting not only the MIA pathway but also other *** at the metabolic level is necessary to increase knowledge on the genetic regulation of the whole metabolic network connected to MIA *** magnetic resonance(NMR)is a very suitable and powerful complementary technique for the identification and quantification of metabolites in the plant ***-based metabolomics has been used in studies of *** for pathway elucidation,understanding stress responses,classification among different cultivars,safety and quality controls of transgenic plants,cross talk between pathways,and diversion of carbon fluxes,with the aim of fully unravelling MIA biosynthesis,its regulation and the function of the alkaloids in the plant from a systems biology point of view.

关键词： Catharanthus roseus monoterpenoid indole alkaloids NMR metabolomics

Molecular insights into AabZIP1-mediated regulation on artemisinin biosynthesis and drought tolerance in Artemisia annua

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1500-1513页

作者： Guoping Shu Yueli tang Mingyuan Yuan Ning Wei Fangyuan Zhang Chunxian Yang Xiaozhong Lan Min Chen kexuan tang Lien Xiang Zhihua Liao Key Laboratory of Eco-environments in Three Gorges Reservoir Region(Ministry of Education) Chongqing Engineering Research Centre for Sweet PotatoSWU-TAAHC Medicinal Plant Joint R&D CentreSchool of Life SciencesSouthwest UniversityChongqing 400715China TAAHC-SWU Medicinal Plant Joint R&D Centre Xizang Agricultural and Husbandry CollegeNyingchi 860000China College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China College of Environmental Science and Engineering China West Normal UniversityNanchong 637009China

Artemisia annua is the main natural source of artemisinin *** ***,extended drought stress severely reduces its biomass and artemisinin production while short-term water-withholding or abscisic acid(ABA)treatment can increase artemisinin ***-responsive transcription factor AabZIP1 and JA signaling AaMYC2 have been shown in separate studies to promote artemisinin production by targeting several artemisinin biosynthesis ***,we found AabZIP1 promote the expression of multiple artemisinin biosynthesis genes including AaDBR2 and AaALDH1,which AabZIP1 does not directly ***,it was found that AabZIP1 up-regulates AaMYC2expression through direct binding to its promoter,and that AaMYC2 binds to the promoter of AaALDH1to activate its *** addition,AabZIP1 directly transactivates wax biosynthesis genes AaCER1and *** biosynthesis of artemisinin and cuticular wax and the tolerance of drought stress were significantly increased by AabZIP1 overexpression,whereas they were significantly decreased in RNAi-AabZIP1 ***,we have uncovered the AabZIP1-AaMYC2 transcriptional module as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis,which may have general *** have also identified AabZIP1 as a promising candidate gene for the development of *** plants with high artemisinin content and drought tolerance in metabolic engineering breeding.

关键词： Artemisia annua AabZIP1 AaMYC2 Artemisinin biosynthesis Drought tolerance Wax biosynthesis

Transcriptional regulation of flavonoid biosynthesis in Artemisia annua by AaYABBY5

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Horticulture Research 2021年第1期8卷 3381-3395页

作者： Sadaf-Ilyas Kayani Qian Shen Saeed-ur Rahman Xueqing Fu Yongpeng Li Chen Wang Danial Hassani kexuan tang Joint International Research Laboratory of Metabolic and Developmental Sciences Plant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong University200240 ShanghaiChina

Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant,anticancer,and antimalarial *** transcriptional regulation of flavonoid biosynthesis in *** has not been *** this study,we identified a YABBY family transcription factor,AaYABBY5,as a positive regulator of anthocyanin and total flavonoid contents in ***5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),chalcone isomerase(CHI),and flavonol synthase(FLS)genes.A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene,with reporters AaPAL,AaCHS,AaCHI,and *** yeast one-hybrid system further confirmed the direct activation of these promoters by *** expression analysis of stably transformed AaYABBY5 overexpression,AaYABBY5 antisense,and control plants revealed a significant increase in the expression of AaPAL,AaCHS,AaCHI,AaFLS,AaFSII,AaLDOX,and AaUFGT in AaYABBY5 overexpression ***,their total flavonoid content and anthocyanin content were also found to ***5 antisense plants showed a significant decrease in the expression of flavonoid biosynthetic genes,as well as a decrease in anthocyanin and total flavonoid *** addition,phenotypic analysis revealed deep purple-pigmented stems,an increase in the leaf lamina size,and higher trichome densities in AaYABBY5 overexpression ***,these data proved that AaYABBY5 is a positive regulator of flavonoid biosynthesis in *** study provides candidate transcription factors for the improvement of flavonoid concentrations in *** and can be further extended to elucidate its mechanism of regulating trichome development.

关键词： transformed ammonia transient

The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua

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Journal of Integrative Plant Biology 2022年第6期64卷 1212-1228页

作者： Zongyou Lv JinXing Li Shi Qiu Fei Qi Hang Su Qitao Bu Rui Jiang kexuan tang Lei Zhang Wansheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003China Department of Pharmaceutical Botany School of PharmacySecond Military Medical UniversityShanghai 200433China Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan‐SJTU‐Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China

The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density;however,the factors affecting trichome development are largely ***,we demonstrate that the *** R2R3 MYB transcription factor TrichomeLess Regulator 1(TLR1)negatively regulates trichome *** ***,TLR1 overexpression lines had 44.7%–64.0%lower trichome density and 11.5%–49.4%lower artemisinin contents and TLR1-RNAi lines had 33%–93.3%higher trichome density and 32.2%–84.0%higher artemisinin contents compared with non-transgenic ***1 also negatively regulates the expression of anthocyanin biosynthetic pathway genes in *** heterologously expressed in Arabidopsis thaliana,TLR1 interacts with GLABROUS3a,positive regulator of trichome development,and represses trichome *** two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox(WOX)protein AaWOX1,which interacts with the LEAFY-like transcription factor ***2 overexpression in Arabidopsis and *** showed that TLR2 reduces trichome development by reducing gibberellin ***,artemisinin contents were 19%–43%lower in TLR2-overexpressing *** plants compared to *** data indicate that TLR1 and TLR2 negatively regulate trichome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields.

关键词： artemisinin Artemisia annua L. LFY MYB transcription factor trichome WOX

The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.

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Journal of Integrative Plant Biology 2024年第8期66卷 1735-1751页

作者： Weizhi He Hang Liu Zhangkuanyu Wu Qing Miao Xinyi Hu Xin Yan Hangyu Wen Yaojie Zhang Xueqing Fu Li Ren kexuan tang Ling Li Frontiers Science Center for Transformative Molecules Joint International ResearchLaboratory of Metabolic and Developmental SciencesKey Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Institute for Agri-Food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin production,but the underlying regulatory mechanism remains *** this study,we demonstrate that ELONGATED HYPOCOTYL5(HY5),a central transcription factor in the light signaling pathway,cannot promote artemisinin biosynthesis on its own,as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their *** analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with ***21 showed a trichome-specific expression ***,the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1,AaMYB108,and AaORA,encoding positive regulators of artemisinin ***,AaHY5 and AaBBX21 physically interacted with the *** E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1).In the dark,AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex,explaining the enhanced production of artemisinin upon light *** study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.

关键词： AaBBX21 AaHY5 AaCOP1 Artemisia annua L. artemisinin biosynthesis light