Transcriptional regulation of flavonoid biosynthesis in Artemisia annua by AaYABBY5
作者机构：Joint International Research Laboratory of Metabolic and Developmental SciencesPlant Biotechnology Research CenterFudan-SJTU-Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong University200240 ShanghaiChina
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：3381-3395页
核心收录：
学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 0902[农学-园艺学] 100602[医学-中西医结合临床] 10[医学]
基 金：This work was supported by the National Key R&D Program of China(2018YFA0900600) the Bill&Melinda Gates Foundation(OPP1199872) the SJTU Global Strategic Partnership Fund(2020SJTU-CORNELL) the SJTU Trans-med Awards Research Program(20190104)
主 题：transformed ammonia transient
摘 要：Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant,anticancer,and antimalarial *** transcriptional regulation of flavonoid biosynthesis in *** has not been *** this study,we identified a YABBY family transcription factor,AaYABBY5,as a positive regulator of anthocyanin and total flavonoid contents in ***5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),chalcone isomerase(CHI),and flavonol synthase(FLS)genes.A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene,with reporters AaPAL,AaCHS,AaCHI,and *** yeast one-hybrid system further confirmed the direct activation of these promoters by *** expression analysis of stably transformed AaYABBY5 overexpression,AaYABBY5 antisense,and control plants revealed a significant increase in the expression of AaPAL,AaCHS,AaCHI,AaFLS,AaFSII,AaLDOX,and AaUFGT in AaYABBY5 overexpression ***,their total flavonoid content and anthocyanin content were also found to ***5 antisense plants showed a significant decrease in the expression of flavonoid biosynthetic genes,as well as a decrease in anthocyanin and total flavonoid *** addition,phenotypic analysis revealed deep purple-pigmented stems,an increase in the leaf lamina size,and higher trichome densities in AaYABBY5 overexpression ***,these data proved that AaYABBY5 is a positive regulator of flavonoid biosynthesis in *** study provides candidate transcription factors for the improvement of flavonoid concentrations in *** and can be further extended to elucidate its mechanism of regulating trichome development.
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