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Standardized surgical approaches to ear surgery in rats

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Journal of Otology 2015年第2期10卷 72-77页

作者： Peng Li Dalian Ding kelei gao Richard Salvi Department of Otolaryngology Head and Neck Surgery The Third Affiliated Hospital of Sun Yat-Sen University Center for Hearing and Deafness University of New York at Buffalo Department of Otolaryngology Head and Neck Surgery Xiangya Hospital Central South University

Objective： To describe several approaches of ear surgeries for experimental studies in rats. Methods： Anesthetized rats were prepared for demonstration of various ear surgery approaches designed to optimize experimental outcomes in studies with specific goals and exposure requirements. The surgical approaches included the posterior tympanum, superior tympanum, inferior tympanum and occipital approaches. Results： The middle ear cavity and inner ear were successfully exposed from different angles via the mentioned surgical approaches. For example, electrode placement for recording of cochlear bioelectric responses was easily achieved through the posterior tympanum or inferior tympanum approach. Alternatively, drug delivery or gene transfection via round window membrane was most easily accomplished using the posterior tympanum approach. Cochlear perfusion of protective or ototoxic drugs was best performed using the inferior tympanum approach. Ossicular chain interruption to induce a prolonged conductive hearing loss was readily achieved using a superior tympanum approach. Lastly, surgical destruction of the endolymphatic sac to induce experimental endolymphatic hydrops was readily performed via an occipital surgical approach.Conclusion： These standardized surgical approaches can be applied in scientific studies of the ear with different purposes covering electro- physiology, conductive hearing loss, intra-cochlear drug perfusion and experimental studies relevant to Meniere＇s disease.

关键词： Rat Middle ear Inner ear Surgical approach

Standardization of experimental animals temporal bone sections

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Journal of Otology 2015年第2期10卷 66-71页

作者： Dalian Ding Jintao Yu Peng Li kelei gao Haiyan Jiang Wenjuan Zhang Hong Sun Shankai Yin Richard Salvi Center for Hearing and Deafness Department of Communicative Disorders and Sciences University of New York at Buffalo Department of Otolaryngology Head and Neck Surgery Sun Ya-sen University Third Hospital Department of Otolaryngology Head and Neck Surgery the Sixth People's Hospital of Shanghai Jiao Tong University Department of Otolaryngology Head and Neck Surgery Central South University Xiangya Hospital Department of Otolaryngology Head and Neck Surgery Union Hospital Tongji Medical College of Huazhong University of Science & Technology

Preparation of the temporal bone for light microscopy is an important step in histological studies of the inner ear. Due to the complexity of structures of the inner ear, it is difficult to measure or compare structures of interest without a commonly accepted standardized measure of temporal bone sections. Therefore, standardization of temporal bone sections is very important for histological assessment of sensory hair cells and peripheral ganglion neurons in the cochlear and vestibular systems. The standardized temporal bone sectioning is oriented to a plane parallel to the outer and internal auditory canals. Sections are collected from the epitympanum to the hypotympanum to reveal layers in the order of the crista ampullaris of the superior and lateral semicircular canals, macula utriculi and macula sacculi, superior vestibular ganglion neurons, macula of saccule and inferior vestibular ganglion neurons, cochlear modiolus, endolymphatic duct and endolymphatic sac, and finally the crista ampullaris of the posterior semicircular canal. Moreover, technical details of preparing for temporal bone sectioning including fixation, decalcification, whole temporal bone staining, embedding penetration, and embedding orientation are also discussed.

关键词： Experimental animal Temporal bone section Temporal bone Collodion embedding Decalcification

Sense to Tune: Engaging Microglia with Dynamic Neuronal Activity

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Neuroscience Bulletin 2023年第3期39卷 553-556页

作者： kelei Cao Yaling Hu Zhihua gao Department of Neurobiology and Department of Neurology of The Second Afliated Hospital Zhejiang University School of MedicineHangzhou 310058China Liangzhu Laboratory MOE Frontier Science Center for Brain Science and Brain-Machine IntegrationState Key Laboratory of Brain-Machine IntelligenceZhejiang University Medical CenterZhejiang UniversityHangzhou 311121China NHC and CAMS Key Laboratory of Medical Neurobiology Zhejiang UniversityHangzhou 310058China

Microglia,the primary resident immune cells in the central nervous system(CNS),continuously survey the microenvironment via their ramified and motile processes to maintain brain homeostasis[1].As immune sentinels,microglia detect exogenous pathogens,elicit inflammation,promote tissue repair,and engage in the onset and progression of brain diseases[1].

关键词： homeostasis inflammation diseases

光子自旋解耦合的人工表面等离激元双功能波前调控

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光学学报 2024年第9期44卷 248-254页

作者：杨清秀席科磊周韶东盛小航张文雅高靖翔王桂芳庄松林程庆庆上海理工大学光电信息与计算机工程学院中国科学院上海光学精密机械研究所微纳光电子功能材料实验室温州医科大学附属衢州医院呼吸科

多功能超表面的研究和开发是光子学的研究热点，光子自旋解耦合的实现对多功能超表面的制备至关重要。利用光子自旋霍尔效应将不同手性的圆偏振光分离，已经取得显著的研究成果。然而，其分离后的圆偏振光的几何相位是互为共轭的，无法... 详细信息

多功能超表面的研究和开发是光子学的研究热点，光子自旋解耦合的实现对多功能超表面的制备至关重要。利用光子自旋霍尔效应将不同手性的圆偏振光分离，已经取得显著的研究成果。然而，其分离后的圆偏振光的几何相位是互为共轭的，无法对两种圆偏振光的相位实现完全独立且自由的调控。本文通过几何相位和共振相位的联合使用，打破了几何相位之间的共轭关系，实现了不同手性圆偏振光的自旋解耦，从而设计出了一款基于人工等离子体极化激元的双功能波前自由调控耦合器。在75 GHz线偏振光源的激励下，耦合器的左右两侧模拟实现了效率分别为41%和37%的聚焦光束和贝塞尔光束。本文为多功能元器件的制备和光子集成的片上器件提供了新的设计方案和思路。

关键词：光子自旋霍尔效应偏振转换超构表面高效耦合器表面波

补阳还五汤治疗气虚血瘀型结缔组织病相关间质性肺病临床研究

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中国中医药现代远程教育 2024年第2期22卷 47-50页

作者：陈美玲苏克雷高卫星江苏省泰兴市中医院呼吸内科江苏泰兴225400 江苏省中西医结合医院呼吸与危重症医学科江苏南京210028

目的观察补阳还五汤加减治疗气虚血瘀型结缔组织病相关间质性肺病(CTD-ILD)的临床效果。方法将60例气虚血瘀型CTD-ILD患者随机分为治疗组和对照组,每组30例。对照组予以常规西医治疗,治疗组在对照组的基础上加用补阳还五汤加减治疗,共治... 详细信息

目的观察补阳还五汤加减治疗气虚血瘀型结缔组织病相关间质性肺病(CTD-ILD)的临床效果。方法将60例气虚血瘀型CTD-ILD患者随机分为治疗组和对照组,每组30例。对照组予以常规西医治疗,治疗组在对照组的基础上加用补阳还五汤加减治疗,共治疗4周。观察2组6 min步行试验、用力肺活量(FVC)。结果治疗后,2组的6 min步行试验、FVC均较治疗前改善,且治疗组优于对照组,差异均有统计学意义(P<0.05)。结论补阳还五汤加减治疗气虚血瘀型CTD-ILD能改善患者的呼吸困难症状,增强肺功能。

关键词：肺痹结缔组织病间质性肺病补阳还五汤中医药疗法

Transformers in medical image analysis

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Intelligent Medicine 2023年第1期3卷 59-78页

作者： kelei He Chen Gan Zhuoyuan Li Islem Rekik Zihao Yin Wen Ji Yang gao Qian Wang Junfeng Zhang Dinggang Shen Medical School of Nanjing University NanjingJiangsu 210093China National Institute of Healthcare Data Science at Nanjing University NanjingJiangsu 210093China BASIRA Laboratory Faculty of Computer and Informatics EngineeringIstanbul Technical UniversityIstanbulTurkey School of Science and Engineering ComputingUniversity of DundeeUK State Key Laboratory for Novel Software Technology Nanjing UniversityNanjingJiangsu 210093China School of Biomedical Engineering ShanghaiTech UniversityShanghai 201210China Department of Research and Development Shanghai United Imaging Intelligence Co.Ltd.Shanghai 200030China Shanghai Clinical Research and Trial Center Shanghai 201703China

Transformers have dominated the field of natural language processing and have recently made an impact in the area of computer vision.In the field of medical image analysis,transformers have also been successfully used in to full-stack clinical applications,including image synthesis/reconstruction,registration,segmentation,detection,and diagnosis.This paper aimed to promote awareness of the applications of transformers in medical image analysis.Specifically,we first provided an overview of the core concepts of the attention mechanism built into transformers and other basic components.Second,we reviewed various transformer architectures tailored for medical image applications and discuss their limitations.Within this review,we investigated key challenges including the use of transformers in different learning paradigms,improving model efficiency,and coupling with other techniques.We hope this review would provide a comprehensive picture of transformers to readers with an interest in medical image analysis.

关键词： Transformer Medical image analysis Deep learning Diagnosis Registration Segmentation Image synthesis Multi-task learning Multi-modal learning Weakly-supervised learning

Brain Macrophages:Close or Distant Relatives?

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Neuroscience Bulletin 2021年第2期37卷 278-280页

作者： kelei Cao Yaling Hu Zhihua gao Department of Neurobiology and Department of Neurology of Second Affiliated Hospital Institute of NeuroscienceNHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang University School of MedicineHangzhou 310058China

The concept of a mononuclear phagocyte system in which tissue-resident macrophages arise from the circulating monocytes has been dogma in immunology for more than half a century[1].While monocytes do give rise to macrophages under certain conditions,recent studies clearly dem on strate that monocytes do not primarily contribute to most adult tissue-resident macrophage pools under the steady state[2].Instead,the majority of adult tissue-resident macrophages are derived from erythromyeloid progenitors(EMPs)in the yolk sac that seed tissue before birth[2].

关键词： primarily progenitor arise

Crystal structure of E. synthetase and ligand revealed key residues coil arglnyl-tRNA binding studies in arginine recognition

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Protein & Cell 2014年第2期5卷 151-159页

作者： kelei Bi Yueting Zheng Feng gao Jianshu Dong Jiangyun Wang Yi Wang Weimin Gong Laboratory of Non-coding RNA Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

The arginyl-tRNA synthetase （ArgRS） catalyzes the esterification reaction between L-arginine and its cog- nate tRNAArg. Previously reported structures of ArgRS shed considerable light on the tRNA recognition mech- anism, while the aspect of amino acid binding in ArgRS remains largely unexplored. Here we report the first crystal structure of E. coli ArgRS （eArgRS） complexed with L-arginine, and a series of mutational studies using isothermal titration calorimetry （ITC）. Combined with previously reported work on ArgRS, our results eluci- dated the structural and functional roles of a series of important residues in the active site, which furthered our understanding of this unique enzyme.

关键词： arginyl-tRNA synthetase amino acyl-tRNAsynthetase isothermal titration calorimetry site-directedmutation X-ray crystal structure E. coil

Non-small cell lung cancers(NSCLCs)oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors

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Signal Transduction and Targeted Therapy 2023年第10期8卷 5069-5084页

作者： Bopei Cui Lifang Song Qian Wang kelei Li Qian He Xing Wu Fan gao Mingchen Liu Chaoqiang An Qiushuang gao Chaoying Hu Xiaotian Hao Fangyu Dong Jiuyue Zhou Dong Liu Ziyang Song Xujia Yan Jialu Zhang Yu Bai Qunying Mao Xiaoming Yang Zhenglun Liang Division of Hepatitis and Enterovirus Vaccines NHC Key Laboratory of Research on Quality and Standardization of Biotech ProductsNMPA Key Laboratory for Quality Research and Evaluation of Biological ProductsInstitute of Biological ProductsNational Institutes for Food and Drug ControlBejingChina National Engineering Technology Research Center for Combined Vaccines WuhanChina Beijing Minhai Biotechnology Co LtdBeijingChina Taibang Biologic Group BeijingChina Changchun Institute of Biological Products Co. LtdChangchunChina Shanghai Institute of Biological Products Co LtdShanghaiChina China National Biotec Group Company Limited BeijingChina

With the continuous in-depth study of the interaction mechanism between viruses and hosts,the virus has become a promising tool in cancer treatment.In fact,many oncolytic viruses with selectivity and effectiveness have been used in cancer therapy.Human enterovirus is one of the most convenient sources to generate oncolytic viruses,however,the high seroprevalence of some enteroviruses limits its application which urges to exploit more oncolytic enteroviruses.

关键词： damage enterovirus cancer