Standardized surgical approaches to ear surgery in rats

作     者：Peng Li Dalian Ding Kelei Gao Richard Salvi 

作者机构：Department of Otolaryngology Head and Neck Surgery The Third Affiliated Hospital of Sun Yat-Sen University Center for Hearing and Deafness University of New York at Buffalo Department of Otolaryngology Head and Neck Surgery Xiangya Hospital Central South University 

出 版 物：《Journal of Otology》 (中华耳科学杂志（英文版）)

年 卷 期：2015年第10卷第2期

页      面：72-77页

核心收录：

学科分类：1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学] 

基　　金：CGIAR-supported International Agricultural Research Centres CIMMYT Centro Internacional de la Papa ICARDA ICRISAT International Service for National Agricultural Research AVRDC - The World Vegetable Center Rice University Centro Internacional de Agricultura Tropical 

主　　题：Rat Middle ear Inner ear Surgical approach 

摘      要：Objective： To describe several approaches of ear surgeries for experimental studies in rats. Methods： Anesthetized rats were prepared for demonstration of various ear surgery approaches designed to optimize experimental outcomes in studies with specific goals and exposure requirements. The surgical approaches included the posterior tympanum, superior tympanum, inferior tympanum and occipital approaches. Results： The middle ear cavity and inner ear were successfully exposed from different angles via the mentioned surgical approaches. For example, electrode placement for recording of cochlear bioelectric responses was easily achieved through the posterior tympanum or inferior tympanum approach. Alternatively, drug delivery or gene transfection via round window membrane was most easily accomplished using the posterior tympanum approach. Cochlear perfusion of protective or ototoxic drugs was best performed using the inferior tympanum approach. Ossicular chain interruption to induce a prolonged conductive hearing loss was readily achieved using a superior tympanum approach. Lastly, surgical destruction of the endolymphatic sac to induce experimental endolymphatic hydrops was readily performed via an occipital surgical ***： These standardized surgical approaches can be applied in scientific studies of the ear with different purposes covering electro- physiology, conductive hearing loss, intra-cochlear drug perfusion and experimental studies relevant to Meniere＇s disease.