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Brassica napus L. Homeodomain Leucine-Zipper Gene BnHB6 Responds to Abiotic and Biotic Stresses

Journal of Integrative Plant Biology 2005年第10期47卷 1236-1248页

作者： Shun-Wu YU Li-Da ZHANG Kai-Jing ZUO Dong-Qin tang Xiao-Fen SUN ke-xuan tang Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China State key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

A homeodomain leucine-zipper （HD-Zip） gene BnHB6 （GenBank accession No. AY336103） was isolated from oilseed rape （Brassica napus L.） following drought treatment through rapid amplification of cDNA ends （RACE）. The full-length cDNA of BnHB6 was 1 611 bp and contained a 936-bp open reading frame encoding 311 amino acids. Sequence analysis indicated that BnHB6 belonged to the HD-Zip I subfamily. High-stringency Southern boltting analysis showed that BnHB6 appeared in rape as a single copy but had homologous genes. Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） analysis revealed that BnHB6 was expressed in several tissues tested under control conditions, but that expression was significantly upregulated in shoots by mannitol, NaCI, cold treatment, anaerobic culture, wounding, H2O2, abscisic acid （ABA）, and salicylic acid （SA） treatments, but not by ultraviolet treatment. Further RT- PCR analysis revealed that BnHB6 was a late-responsive gene, the expression of which was not activated by NaCI, cold treatment, H2O2, ABA, and SA at an early time point （20 min） of treatment in the shoot. However, after a certain period of treatment, the induced expression culminated and then declined until the next peak occurred. Tissue-specific analysis revealed that BnHB6 was expressed at certain levels in the roots, shoots, and flowers, and the roots were found to respond to the osmotic stimuli more rapidly than shoots to increase the expression of BnHB6. The present study implies that BnHB6 plays a positive role as a regulator of biotic and abiotic stresses on growth during seedling establishment.

关键词： Brassica napus homeodomain leucine-zipper gene water stress.

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Cloning and Expressional Studies of the Voltage-dependent Anion Channel Gene from Brassica rapa L.

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Journal of Integrative Plant Biology 2006年第2期48卷 197-203页

作者： Jiang Wang Li-Da Zhang Kai-Jing ZUO Hong-Mei Qian You-Fang Cao ke-xuan tang Plant Biotechnology Research Center Fudan-Shanghai Jiao Tong University-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200030 China State key Laboratory of Genetic Engineering Fudan-Shanghai Jiao Tong University-Nottingham Plant Biotechnology R＆D Center School of Life Sciences Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

The voltage-dependent anion channel （VDAC） plays an essential role in the permeability of mltochondrial membrane. In the present study, we isolated a novel VDAC gene （brvdac） based on the assembly of expressed sequence tag sequences from Brassica rapa L. and explored its differential expression patterns In growth, tissues, abiotlc stress, and stress recovery. Results of a tissue-specific expression study in young seedlings Indicated that, of all tissues tested, brvdac expression was the highest in the leaves. Under cold, drought, and salt stresses, brvdac expression showed a transient Increase, and then returned to normal levels when the stress was removed. When plants were exposed to heat shock, there was no Increase in brvdac expression, whereas during recovery a quick and considerable increase in expression was observed. These observations indicate that dissimilar modulations of brvdactranscription may occur when plant cells encounter heat shock and the other three types of stress. In addition, phylogenetic analysis Implied that an earlier duplication of vdac probably occurred before the divergence between monocotyledons and dicotyledons.

关键词： Brassica rapa expression heat shock stress voltage-dependent anion channel.

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Terpenoid Indole Alkaloids Biosynthesis and Metabolic Engineering in Catharanthus roseus

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Journal of Integrative Plant Biology 2007年第7期49卷 961-974页

作者： Dong-Hui Liu Hong-Bin Jin Yu-Hui Chen Li-Jie Cui Wei-Wei Ren Yi-Fu Gong ke-xuan tang Shanghai key Laboratory of Agrobiotechnology Plant Biotechnology Research Center Fudan-Shanghai Jiaotong University ( SJTU)-Nottingham Plant Biotechnology R & D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Department of Biochemistry and Molecular Biology Shenyang Medical College Shenyang 110034 China Faculty of Life Sciences and Biotechnology Ningbo University Ningbo 315211 China State key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R & D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

Catharanthus roseus L. （Madagascar periwinkle） biosynthesizes a diverse array of secondary metabolites including anticancer dimeric alkaloids （vinblastine and vincristine） and antihypertensive alkaloids （ajmalicine and serpentine）. The multi-step terpenoid indole alkaloids （TIAs） biosynthetic pathway in C. roseus is complex and is under strict molecular regulation. Many enzymes and genes involved in the TIAs biosynthesis have been studied in recent decades. Moreover, some regulatory proteins were found recently to control the production of TIAs in C. roseus. Based on mastering the rough scheme of the pathway and cloning the related genes, metabolic engineering of TIAs biosynthesis has been studied in C. roseus aiming at increasing the desired secondary metabolites in the past few years. The present article summarizes recent advances in isolation and characterization of TIAs biosynthesis genes and transcriptional regulators involved in the second metabolic control in C. roseus. Metabolic engineering applications in TIAs pathway via overexpression of these genes and regulators in C. roseus are also discussed.

关键词： biosynthesis pathway Catharanthus roseus metabolic engineering terpenoid indole alkaloids.

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A New Farnesyl Diphosphate Synthase Gene from Taxus media Rehder:Cloning,Characterization and Functional Complementation

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Journal of Integrative Plant Biology 2006年第6期48卷 692-699页

作者： Zhi-Hua Liao Min Chen Yi-Fu Gong Zhu-Gang Li Kai-Jing Zuo Peng Wang Feng Tan Xiao-Fen Sun ke-xuan tang State key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China Laboratory of Natural Products and Metabofic Engineering Institute of Biotechnology Chongqing Sweetpotato Research Center School of Life Sciences Southwest University Chongqing 400715 China Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Faculty of Life Sciences and Biotechnology Ningbo University Ningbo 315211 China

Farnesyl dlphosphate synthase （FPS; EC 2.***.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting step In terpenold biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl dlphosphate synthase from a gymnosperm plant species, Taxus media Rehder, designated as TmFPS1. The full-length cDNA of TmFPS1 （GenBank accession number： AY461811） was 1 464 bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptlde with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Biolnformatlc analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetlc analysis showed that farnesyl dlphosphate synthases can be divided Into two groups： one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature Is the arrangement of 13 core helices around a large central cavity In which the catalytic reaction takes place. Our blolnformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPSgene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, Including the needles, stems and roots of T. media. Subsequently, functional complementatlon with TmFPS1 in a FPS-deflclent mutant yeast demonstrated that TmFPS1 did encode farnesyl dlphosphate synthase, which rescued the yeast mutant. This study will be helpful In future Investigations aiming at understanding the detailed role of FPS In terpenold biosynthesis flux control at the molecular genetic level.

关键词： farnesyl diphosphate synthase rapid amplification of cDNA ends Taxus media yeast complementation

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Cell polarization in ischemic stroke: molecular mechanisms and advances

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Neural Regeneration Research 2025年第3期20卷 632-645页

作者： Yuanwei Li Xiaoxiao Xu xuan Wu Jiarui Li Shiling Chen Danyang Chen Gaigai Li Zhouping tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is a cerebrovascular disease associated with high mortality and disability rates. Since the inflammation and immune response play a central role in driving ischemic damage, it becomes essential to modulate excessive inflammatory reactions to promote cell survival and facilitate tissue repair around the injury site. Various cell types are involved in the inflammatory response, including microglia, astrocytes, and neutrophils, each exhibiting distinct phenotypic profiles upon stimulation. They display either proinflammatory or anti-inflammatory states, a phenomenon known as ‘cell polarization.’ There are two cell polarization therapy strategies. The first involves inducing cells into a neuroprotective phenotype in vitro, then reintroducing them autologously. The second approach utilizes small molecular substances to directly affect cells in vivo. In this review, we elucidate the polarization dynamics of the three reactive cell populations(microglia, astrocytes, and neutrophils) in the context of ischemic stroke, and provide a comprehensive summary of the molecular mechanisms involved in their phenotypic switching. By unraveling the complexity of cell polarization, we hope to offer insights for future research on neuroinflammation and novel therapeutic strategies for ischemic stroke.

关键词： astrocyte polarization immune regulation inflammation ischemic injury microglia polarization neutrophil polarization signaling pathways stroke

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Molecular cloning, characterization, and promoter analysis of the isochorismate synthase （AalCS1） gene from Artemisia annua

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第8期18卷 662-673页

作者： Lu-yao WANG Ying ZHANG Xue-qing FU Ting-ting ZHANG Jia-wei MA Li-da ZHANG Hong-mei QIAN ke-xuan tang Shan LI Jing-ya ZHAO Plant Bioteehnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China School of Bioscience and Bioengineering South China University of Technology Guangzhou 510006 China

Isochorismate synthase(ICS) is a crucial enzyme in the salicylic acid(SA) synthesis pathway. The full-length complementary DNA(cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene, named AaICS1, contained a 1710-bp open reading frame, which encoded a protein with 570 amino acids. Bioinformatics and comparative study revealed that the polypeptide protein of AaICS1 had high homology with ICSs from other plant species. Southern blot analysis suggested that AaICS1 might be a single-copy gene. Analysis of the 1470-bp promoter of AaICS1 identified distinct cis-acting regulatory elements, including TC-rich repeats, MYB binding site(MBS), and TCA-elements. An analysis of AaICS1 transcript levels in multifarious tissues of A. annua using quantitative real-time polymerase chain reaction(qRT-PCR) showed that old leaves had the highest transcription levels. AaICS1 was up-regulated under wounding, drought, salinity, and SA treatments. This was corroborated by the presence of the predicted cis-acting elements in the promoter region of AaICS1. Overexpressing transgenic plants and RNA interference transgenic lines of AaICS1 were generated and their expression was compared. High-performance liquid chromatography(HPLC) results from leaf tissue of transgenic A. annua showed an increase in artemisinin content in the overexpressing plants. These results confirm that AaICS1 is involved in the isochorismate pathway.

关键词： Salicylic acid Artemisia annua L. Quantitative real-time polymerase chain reaction (qRT-PCR) Isochorismate synthase

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Cloning and molecular characterization of a novel lectin gene from Pinellia ternata

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Cell Research 2003年第4期13卷 301-308页

作者： JIAN HONG YAO, XIU YUN ZHAO, ZHI HUA LIAO, JUAN LIN, ZHONG HAI CHEN, FEI CHEN, JUN SONG, XIAO FEN SUN, ke xuan tang State key Laboratory of Genetic Engineering, School of Life Sciences, Morgan- Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, Fudan University, Shanghai 200433, ChinaFudan-SJTU-Nottingham Plant Biotechnology R and D Center, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200030, China State key Laboratory of Genetic Engineering School of Life Sciences Morgan-Tan International Center for Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R and D Center Fudan University Shanghai China Fudan-SJTU-Nottingham Plant Biotechnology R and D Center Plant Biotechnology Research Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai China

The full-length cDNA of Pinellia ternata agglutinin (PTA) was cloned from inflorescences using RACE PCR. Through comparative analysis of PTA gene (pta) and its deduced amino acid sequence with those of other Araceae species, pta was found to encode a precursor lectin with signal peptide and to have extensive homology with those of other Araceae species. PTA was a heterotetrameric mannose-binding lectin with three mannose-binding boxes like lectins from other Araceae and Amaryllidaceae species. Southern blot analysis of the genomic DNA revealed that pta belonged to a low-copy gene family. Northern blot analysis demonstrated that pta constitutively expressed in various plant tissues including root, leaf, stem and inflorescence. The pta cDNA sequence encoding for mature PTA protein was cloned into pET-32a plasmid and the resulting plasmid, pET-32a-PTA containing Trx-PTA fusion protein, was investigated for the expression in E. coli BL21. SDS-PAGE gel analysis showed that the Trx-PTA fusion protein was succ

关键词： araceae cDNA cloning lectin Pinellia ternata RACE-PCR.

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Regulation of Jasmonate-Mediated Artemisinin Biosynthesis by an AaORA-AaDAB Complex in Artemisia annua

Regulation of Jasmonate-Mediated Artemisinin Biosynthesis by...

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第十届上海市植物生物学青年学术研讨会

作者： Ya-nan Ma Ling Li Xue-qing Fu Pu Shi Qian Shen Xiao-long Hao Yue-Li tang Qian He Li-hui Xie Ting-xiang Yan Ming-hui Chen Qi-fang Pang Xiao-fen Sun ke-xuan tang Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University

Artemisia annua produces a valuable medicinal component,artemisinin,a sesquiterpene lactone widely used in malaria *** jasmonate(JA)is well-known to induce the expression of all the crucial enzymes in artemisinin

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Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： Xiahong tang Nan Zheng Qingming Lin Yan You Zheng Gong Yangping Zhuang Jiali Wu Yu Wang Hanlin Huang Jun ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

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2011—2020年广州市肺结核死亡趋势及年龄-时期-队列模型分析

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中国防痨杂志 2025年第1期47卷 44-50页

作者：李雪秋刘群唐科吴迪广州市胸科医院结核病防治所一分所广州510095 广东省生物制品与药物研究所药理研究室广州510440 广州市疾病预防控制中心慢性非传染性疾病预防控制部广州510440 广东医科大学公共卫生学院东莞523808

目的:分析2011—2020年广州市肺结核患者的死亡情况和死亡率变化趋势,为制定防控策略和措施提供科学依据。方法:采用Joinpoint回归模型分析2011—2020年广州市全年龄段人群肺结核死亡率变化趋势,并计算年度变化百分比(annual percent ch... 详细信息

目的:分析2011—2020年广州市肺结核患者的死亡情况和死亡率变化趋势,为制定防控策略和措施提供科学依据。方法:采用Joinpoint回归模型分析2011—2020年广州市全年龄段人群肺结核死亡率变化趋势,并计算年度变化百分比(annual percent change,APC)和平均年度变化百分比(average annual percent change,AAPC);使用年龄-时期-队列模型分析广州市20~85岁人群肺结核死亡风险的年龄、时期和队列效应。结果:广州市2011—2020年报告肺结核死亡患者2144例,年均报告标化死亡率为2.57/10万;男性肺结核标化死亡率为4.19/10万,约为女性标化死亡率(0.96/10万)的4.4倍。10年间广州市肺结核年龄标化死亡率总体呈现下降趋势,AAPC为-4.15%(95%CI:-6.41%~-1.85%,P=0.003);其中,女性肺结核死亡率下降速度高于男性,AAPC分别为-6.72%(95%CI:-10.38%~-2.92%,P=0.004)和-3.55%(95%CI:-6.19%~-0.85%,P=0.017)。在不同年龄段人群中,0~49岁和≥65岁年龄段人群肺结核死亡率均呈现明显下降趋势,AAPC分别为-7.05%(95%CI:-11.52%~-2.34%,P=0.009)和-3.94%(95%CI:-6.22%~-1.62%,P=0.005)。年龄-时期-队列模型的年龄效应显示,随着年龄的增长,肺结核死亡风险不断上升,≥65岁人群尤为显著,发病率由66~67岁年龄组的2.93/10万增长至84~85岁年龄组的6.10/10万。时期效应显示,肺结核死亡风险随着时期的变化总体呈现下降趋势,相对危险度(risk ratio,RR)从2011—2012年时期组的1.35下降至2019—2020年时期组的0.81。队列效应显示,越晚出生的人群肺结核死亡风险越低,RR值从1926—1927年出生队列的5.91降低至1998—1999年出生队列的0.16。结论:广州市肺结核死亡率总体呈不断下降的趋势,但男性和女性肺结核死亡率差距较大。需重点关注男性和≥65岁年龄段的老年人群,并实施针对性的防控和治疗措施。

关键词：结核,肺死亡率回归分析队列研究

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