Cloning and molecular characterization of a novel lectin gene from Pinellia ternata
Cloning and molecular characterization of a novel lectin gene from Pinellia ternata作者机构：State Key Laboratory of Genetic Engineering School of Life Sciences Morgan-Tan International Center for Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R and D Center Fudan University Shanghai China Fudan-SJTU-Nottingham Plant Biotechnology R and D Center Plant Biotechnology Research Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2003年第13卷第4期
页 面：301-308页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：CommercializationProject, China Technology Committee Technology Collaboration Fund
主 题：araceae cDNA cloning lectin Pinellia ternata RACE-PCR.
摘 要：The full-length cDNA of Pinellia ternata agglutinin (PTA) was cloned from inflorescences using RACE PCR. Through comparative analysis of PTA gene (pta) and its deduced amino acid sequence with those of other Araceae species, pta was found to encode a precursor lectin with signal peptide and to have extensive homology with those of other Araceae species. PTA was a heterotetrameric mannose-binding lectin with three mannose-binding boxes like lectins from other Araceae and Amaryllidaceae species. Southern blot analysis of the genomic DNA revealed that pta belonged to a low-copy gene family. Northern blot analysis demonstrated that pta constitutively expressed in various plant tissues including root, leaf, stem and inflorescence. The pta cDNA sequence encoding for mature PTA protein was cloned into pET-32a plasmid and the resulting plasmid, pET-32a-PTA containing Trx-PTA fusion protein, was investigated for the expression in E. coli BL21. SDS-PAGE gel analysis showed that the Trx-PTA fusion protein was succ
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