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浙江天台山七子花群落主要植物种类的光合特性

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生态学报 2002年第10期22卷 1645-1652页

作者：金则新柯世省台州师范专科学校浙江临海317000

对分布在浙江天台山的七子花群落主要植物种类的光合生理生态特性进行了研究。结果表明 :夏季 ,位于群落冠层上层、林窗、林缘的七子花及主要伴生植物叶片的光合日进程均呈“双峰”曲线 ,有明显的光合“午休”现象。而位于冠层中、下层... 详细信息

对分布在浙江天台山的七子花群落主要植物种类的光合生理生态特性进行了研究。结果表明 :夏季 ,位于群落冠层上层、林窗、林缘的七子花及主要伴生植物叶片的光合日进程均呈“双峰”曲线 ,有明显的光合“午休”现象。而位于冠层中、下层以及林下的七子花、草本植物 ,其光合日进程曲线平缓。从冠层上层到下层 ,七子花叶片的日均净光合速率 ( Pn)呈下降趋势。林窗、林缘的七子花小树叶片日均 Pn比林下大 ,冠层上层的七子花中树叶片与林窗、林缘的小树叶片日均Pn差异不显著。七子花在不同生境中与伴生植物相比 ,日均 Pn较低 ,光合能力较弱。同一植株七子花冠层上层叶片叶绿素含量最低 ,下层次之 ,中层最高。阴生草本植物在弱光下仍有很高的叶绿素含量。植物叶片的光合能力大小与叶绿素含量高低不呈正相关。相对常绿树种而言 ,七子花光补偿点较高、光饱和点较低。

关键词：浙江天台山七子花群落植物种类光合特性

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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七子花土壤微生物数量及呼吸速率的季节动态

微生物学通报 2003年第4期30卷 6-9页

作者：张崇邦金则新柯世省王锦文浙江省台州学院生物系临海317000

研究濒危植物七子花群落土壤微生物的生长及其呼吸速率的季节动态 ,结果表明 :根际土壤细菌、放线菌 ,根围土壤细菌、真菌、放线菌的数量和土壤总呼吸速率具有相似的季节变化规律 :全年呈单峰曲线变化 ,最大值均出现在 9月份 ,但根际土... 详细信息

研究濒危植物七子花群落土壤微生物的生长及其呼吸速率的季节动态 ,结果表明 :根际土壤细菌、放线菌 ,根围土壤细菌、真菌、放线菌的数量和土壤总呼吸速率具有相似的季节变化规律 :全年呈单峰曲线变化 ,最大值均出现在 9月份 ,但根际土壤真菌数量的最大值出现在 1 0月份。土壤微生物数量受土壤含水量和温度的影响较大 ;土壤总呼吸速率不仅与土壤含水量和温度存在密切关系 ,而且与土壤微生物数量显著相关。

关键词：七子花群落土壤微生物土壤总呼吸速率

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六阶光滑逼近函数的数值方法及其应用

数值计算与计算机应用 1987年第2期 96-103页

作者：张可村施兴中西安交通大学

一、数学模型的建立在工程实际问题中,有许多机械零部件的外形设计都需要从一些离散数据中拟合一光滑曲线或曲面,由于零部件的功能不同,对其外形的要求也不同,自然所采用的设计方法也不一样,本文根据一类机械零件的外型特点和性能要求... 详细信息

一、数学模型的建立在工程实际问题中,有许多机械零部件的外形设计都需要从一些离散数据中拟合一光滑曲线或曲面,由于零部件的功能不同,对其外形的要求也不同,自然所采用的设计方法也不一样,本文根据一类机械零件的外型特点和性能要求,把外型设计,归结为如下泛函的条件极值:

关键词：数值方法对角阵光滑逼近函数方程组联立方程凸轮升程接近程度条件极值极值(数学) 含参变量积分导数变化率张可

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2016年大庆市医院感染现患率调查分析

中华医院感染学杂志 2018年第6期28卷 946-948页

作者：江敏袁可欣孙薇石永丽郭颖李姗李杨大庆油田总医院感染管理处黑龙江大庆163000

目的调查大庆市医院感染现状、抗菌药物使用情况、病原菌检出和感染相关因素,为预防控制医院感染降低医院感染率提供依据。方法采用横断面调查方法,对全市各医院的感染专职人员进行相关培训、统一标准,对2016年5月18日0时-24时住院患者... 详细信息

目的调查大庆市医院感染现状、抗菌药物使用情况、病原菌检出和感染相关因素,为预防控制医院感染降低医院感染率提供依据。方法采用横断面调查方法,对全市各医院的感染专职人员进行相关培训、统一标准,对2016年5月18日0时-24时住院患者进行现患率调查,填写调查表并进行资料整理统计分析。结果共调查大庆市17家医院,应调查住院患者7327例,实际调查7274例,实查率为99.28%;发生医院感染140例,现患率为1.92%;社区感染1404例,现患率为19.30%;各级医院发生医院感染部位均以下呼吸道感染为主,三级医院感染部位前三位依次为下呼吸道、上呼吸道及泌尿道,分别占52.05%,19.18%,12.33%;标本送检率为61.22%;抗菌药物使用率为25.17%,其中治疗用药占76.08%,预防用药占7.10%,治疗加预防用药占16.82%。结论医院感染现患率调查真实反映了大庆市医院感染状况和发展趋势,应针对性加强医院感染重点科室监测,加大抗菌药物管理,有效降低医院感染率。

关键词：医院感染现患率调查分析

Prediction and optimization of flue pressure in sintering process based on SHAP

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 346-359页

作者： Mingyu Wang Jue Tang Mansheng Chu Quan shi Zhen Zhang School of Metallurgy Northeastern UniversityShenyang 110819China Liaoning Low-Carbon Steelmaking Technology Engineering Research Center Northeastern UniversityShenyang 110819China Engineering Research Center of Frontier Technologies for Low-Carbon Steelmaking(Ministry of Education) Shenyang 110819China

Sinter is the core raw material for blast *** pressure,which is an important state parameter,affects sinter *** this paper,flue pressure prediction and optimization were studied based on the shapley additive explanation(SHAP)to predict the flue pressure and take targeted adjustment ***,the sintering process data were collected and processed.A flue pressure prediction model was then constructed after comparing different feature selection methods and model algorithms using SHAP+extremely random-ized trees(ET).The prediction accuracy of the model within the error range of±0.25 kPa was 92.63%.SHAP analysis was employed to improve the interpretability of the prediction *** effects of various sintering operation parameters on flue pressure,the relation-ship between the numerical range of key operation parameters and flue pressure,the effect of operation parameter combinations on flue pressure,and the prediction process of the flue pressure prediction model on a single sample were analyzed.A flue pressure optimization module was also constructed and analyzed when the prediction satisfied the judgment *** operating parameter combination was then *** flue pressure was increased by 5.87%during the verification process,achieving a good optimization effect.

关键词： sintering process flue pressure shapley additive explanation prediction optimization

3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： ke Yao Gaoying Hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong Li Yuewei Chen Nian Liu Jing He Jue shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

乳液单引物PCR构建随机ssDNA文库方法的建立及优化

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临床检验杂志 2016年第11期34卷 823-826页

作者：邵可可史新惠崔蕾蕾马达邵启祥江苏大学医学院江苏镇江212013 盐城市第一人民医院检验科江苏盐城224005

目的建立并优化乳液单引物PCR扩增法,高效构建高质量随机ss DNA文库,以提高从随机文库中筛选适配子的几率。方法构建长90 bp（含52 bp随机序列）寡核苷酸文库,用乳液PCR转换成ds DNA文库,再以ds DNA文库为模板,分别用乳液单引物PCR和常... 详细信息

目的建立并优化乳液单引物PCR扩增法,高效构建高质量随机ss DNA文库,以提高从随机文库中筛选适配子的几率。方法构建长90 bp（含52 bp随机序列）寡核苷酸文库,用乳液PCR转换成ds DNA文库,再以ds DNA文库为模板,分别用乳液单引物PCR和常规单引物PCR扩增制备ss DNA文库,并对二者进行比较。通过改变模板、聚合酶、d NTP和引物浓度优化乳液单引物PCR反应条件。结果乳液PCR能高效扩增出ds DNA文库且无副产物;常规单引物PCR构建ss DNA文库时,15个循环时即有大量副产物产生,且与产物条带混合一起无法分离;而乳液单引物PCR从15～35个循环均无副产物生成,且产物量多于常规单引物PCR。在优化乳液单引物PCR时,模板浓度对产物量影响最大,引物浓度对副产物的量影响最大。结论乳液单引物PCR能显著提高产物量并控制副产物的量,可提高指数富集配体进化（SELEX）技术筛选效率。

关键词：乳液PCR 乳液单引物PCR 适配子指数富集配体进化技术随机ss DNA文库

Gauss Sum of Index 4:(2)Non-cyclic Case

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Acta Mathematica Sinica,English Series 2006年第3期22卷 833-844页

作者： Jing YANG shi xin LUO ke Qin FENG Department of Mathematical Science Tsinghua University

Assume that m ≥ 2, p is a prime number, （m,p（p - 1）） = 1,-1 not belong to 〈p〉 belong to （Z/mZ）^＊ and [（Z/mZ）^＊：〈p〉]=*** this paper, we calculate the value of Gauss sum G（X）=∑x∈F^＊x（x）ζp^T（x） over Fq,where q=p^f,f=φ（m）/4,x is a multiplicative character of Fq and T is the trace map from Fq to *** our assumptions,G（x） belongs to the decomposition field K of p in Q（ζm） and K is an imaginary quartic abelian unmber *** the Galois group Gal（K/Q） is cyclic,we have studied this cyclic case in anotyer paper：＂Gauss sums of index four：（1）cyclic case＂（accepted by Acta Mathematica Sinica,2003）.In this paper we deal with the non-cyclic case.

关键词： Gauss sum Stickelberger Theorem Davenport-Hawse formula class number of imaginary quadratic field