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General Regulatory Patterns of Plant Mineral Nutrient Depletion as Revealed by serat Quadruple Mutants Disturbed in Cysteine Synthesis

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Molecular Plant 2010年第2期3卷 438-466页

作者： Mutsumi Watanabe Hans-Michael Hubberten kazuki saito Rainer Hoefgen Graduate School of Pharmaceutical Sciences Chiba University 1-33 Yayoi-cho Inage-ku Chiba 263-8522 Japan Max-Planck-lnstitut fuer Molekulare Pflanzenphysiologie Wissenschaftspark Golm Am Muehlenberg 1 14476 Potsdam-Golm RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan

Sulfate is an essential macronutrient for plants. Plants have developed strategies to cope with sulfate deficiency, and other nutrient ion limitations. However, the regulation of these adaptive responses and the coordinating signals that underlie them are still poorly characterized. O-acetylserine （OAS） is a marker metabolite of sulfate starvation and has been speculated to have a signaling function. OAS is synthesized by the enzyme serine acetyltransferase （SERAT）, which is encoded by five distinct genes in Arabidopsis. We investigated quadruple knockout mutants of SERAT that retained only one functional isoform. These mutants displayed symptoms of sulfate starvation. Furthermore, some of them displayed phenotypes typical of prolonged sulfate starvation, in particular, developmental programs associated with senescence or stress responses. Thus, we compared metabolite and transcriptome data from these mutants with N-, P-, K-, and S-depleted plants. This revealed many similarities with general nutrient-depletion-induced senescence （NuDIS）, in- dicating the recruitment of existing regulatory programs for nutrient-starvation responses. Several candidate genes that could be involved in these processes were identified, including transcription factors and other regulatory proteins, as well as the functional categories of their target genes. These results outline components of the regulatory network controlling plant development under sulfate stress, forming a basis for further investigations to elucidate the complete network. In turn, this will advance our broader understanding of plant responses to a range of other nutrient stresses.

关键词： Abiotic/environmental stress ion/metabolite sensing senescence functional genomics Arabidopsis sulfur.

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Natural Convection Melting in a Rectangular Heat Storage Tank of Carbon Nanotube Dispersed Latent Heat Storage Material

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Journal of Thermal Science 2024年第3期33卷 847-855页

作者： Shin-ichi MORITA Tomoya saito Kazunori TAKAI Yasutaka HAYAMIZU Naoto HARUKI Division of Mechanical and Electrical Engineering Kitami Institute of Technology165 Koen-choKitami-shiHokkaido090-8507Japan National Institute of Technology Yonago College4448 Hikona-cyoYonago-shiTottori683-8502Japan Okayama Prefectural University 111 KubokiSoja-shiOkayama719-1197Japan

A dispersion system fluid can convect even if the dispersoid is a solid phase.Therefore,heat exchange performance can be improved while maintaining fluidity using a material with high thermal conductivity as the dispersoid.This study presents the melting performance evaluation results of a latent heat storage material with a carbon nanotube(CNT)dispersion system with high thermal conductivity,which enhances the thermal conductivity of the latent heat storage material and does not limit free convection.Increasing the thermal conductivity and enhancing the melting convection of the heat storage material result in increased latent heat storage speed.In this study,the thermal conductivity of the latent heat storage material was successfully increased by dispersing CNTs in the material.When 0.1%(in mass)of multi-wall CNT(MWCNT)was dispersed in a paraffin-based latent heat storage material,the shear stress increased by 1.5 times at a shear rate of 500 s^(-1),while taking into account the potential effects of convective inhibition.Therefore,a latent heat storage experiment was conducted in a rectangular heat storage tank using the CNT dispersion composition ratio as a parameter.A rectangular vessel with a heated vertical surface was used for the latent heat storage experiment.The melting speed was determined by comparing the amount of latent heat stored in a CNT-dispersed latent heat storage material and a single-phase latent heat storage material sample.The experimental results show that the time required for the latent heat storage material to completely melt in the heat storage tank was the shortest for the single-phase latent heat storage material sample.However,the fastest melting progress was observed for the sample with 0.02%(in mass)MWCNT content in the melting rate range of up to approximately 40%in the tank.The results indicate that this phenomenon is caused by the difference in the melting rates in the upper part of the tank.The generated data are useful for determining the shape and heat transfer surface arr

关键词： latent heat storage phase change carbon nano tube free convection heat transfer

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Hyperacute experimental model of rat lung transplantation using a coronary shunt cannula

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World Journal of Transplantation 2024年第2期14卷 155-161页

作者： Munehisa Takata Yusuke Tanaka Daisuke saito Shuhei Yoshida Isao Matsumoto Department of Thoracic Surgery Kanazawa UniversityKanazawa 920-8641IshikawaJapan

BACKGROUND Lung transplantation is a well-established treatment of end-stage lung disease.A rodent model is an inexpensive way to collect biological data from a living model after lung transplantation.However,mastering the surgical technique takes time owing to the small organ size.AIM To conduct rat lung transplantation using a shunt cannula(SC)or modified cannula(MC)and assess their efficacy.METHODS Rat lung transplantation was performed in 11 animals in the SC group and 12 in the MC group.We devised a method of rat lung transplantation using a coronary SC for coronary artery bypass surgery as an anastomosis of pulmonary arteri-ovenous vessels and bronchioles.The same surgeon performed all surgical proce-dures in the donor and recipient rats without using a magnifying glass.The success rate of lung transplantation,operating time,and PaO2 values were com-pared after 2-h reperfusion after transplantation.RESULTS Ten and 12 lungs were successfully transplanted in the SC and MC groups,respectively.In the SC group,one animal had cardiac arrest within 1 h after reperfusion owing to bleeding during pulmonary vein anastomosis.The opera-ting time for the removal of the heart-lung block from the donor and preparation of the left lung graft was 26.8±2.3 and 25.7±1.3 min in the SC and MC groups,respectively(P=0.21).The time required for left lung transplantation in the recipients was 37.5±2.8 min and 35.9±1.4 min in the SC and MC groups,respectively(P=0.12).PaO2 values at 2 h after reperfusion were 456.2±25.5 and INTRODUCTION Lung transplantation is a well-established treatment of end-stage lung disease.Many immune and non-immune mech-anisms in lung transplantation are highly complex,and post-transplant complications such as infections and primary and chronic lung allograft dysfunction must be reduced to improve survival.Therefore,there is a need for immunological and pathophysiological analyses using animal lung transplantation models.The rat lung transplantation model was first reported in 1971[1],followed by the Mizuta

关键词： Lung transplantation Rat Shunt cannula Modified cannula Reperfusion

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Deciphering Starch Quality of Rice Kernels Using Metabolite Profiling and Pedigree Network Analysis

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Molecular Plant 2012年第2期5卷 442-451页

作者： Miyako Kusano Atsushi Fukushima Naoko Fujita Yozo Okazaki Makoto Kobayashi Naoko Fujita Oitome Kaworu Ebana kazuki saito RIKEN Plant Science Center Tsurumi Yokohama 230-0045 Japan KIHARA Institute for Biological Research Yokohama City University Totsuka Yokohama 244-0813 Japan Department of Biological Production Akita Prefectural University Akita 010-0195 Japan National Institute of Agrobiological Sciences 2-1-2 Kannondai Tsukuba Ibaraki 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Chiba 260-8675 Japan

The physiological properties of rice grains are immediately obvious to consumers. High-coverage metabolomic characterization of the rice diversity research set predicted a negative correlation between fatty acid and lipid levels and amylose/total starch ratio （amylose ratio）, but the reason for this is unclear. To obtain new insight into the relationships among the visual phenotypes of rice kernels, starch granule structures, amylose ratios, and metabolite changes, we investigated the metabolite changes of five Japonica cultivars with various amylose ratios and two knockout mutants （e 1, a Starch synthase Ilia （SSIIla）-deficient mutant and the SSIIla/starch branching enzyme （BE） double-knockout mutant 4019） by using mass spectrometry-based metabolomics techniques. Scanning electron microscopy clearly showed that the two mutants had unusual starch granule structures. The metabolomic compositions of two cultivars with high amylose ratios （Hoshiyutaka and Yumetoiro） exhibited similar patterns, while that of the double-knockout mutant, which has an extremely high amylose ratio, differed. Rice pedigree network analysis of the cultivars and the mutants provided insight into the association between metabolic-trait properties and their underlying genetic basis in rice breeding in Japan. Multidimensional scaling analysis revealed that the Hoshiyutaka and Yumetoiro cultivars were Indica-like, yet they are classified as Japonica subpopulations. Exploring metabolomic traits is a powerful way to follow rice genetic traces and breeding history.

关键词： starch quality traits amylose ratio metabolite profiling pedigree networks rice.

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Assessment of corrected time-step method for nominal ionospheric gradient calculation: A comparative analysis with spatial approaches

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Earth and Planetary Physics 2024年第4期8卷 641-649页

作者： Slamet Supriadi Prayitno Abadi Susumu saito Harry Bangkit Dwiko Unggul Prabowo Adi Purwono Ginaldi Ari Nugroho Research Center for Smart Mechatronics National Research and Innovation Agency(BRIN)BandungIndonesia Research Center for Climate and Atmosphere BRINBandungIndonesia School of Electrical Engineering Telkom UniversityKab.BandungIndonesia Electronic Navigation Research Institute National Institute of MaritimePort and Aviation TechnologyTokyoJapan Research Center for Space BRINBandungIndonesia

The effect of ionospheric delay on the ground-based augmentation system under normal conditions can be mitigated by determining the value of the nominal ionospheric gradient(σvig).The nominal ionospheric gradient is generally obtained from Continuously Operating Reference Stations data by using the spatial single-difference method(mixed-pair,station-pair,or satellite-pair)or the temporal single-difference method(time-step).The time-step method uses only a single receiver,but it still contains ionospheric temporal variations.We introduce a corrected time-step method using a fixed-ionospheric pierce point from the geostationary equatorial orbit satellite and test it through simulations based on the global ionospheric model.We also investigate the effect of satellite paths on the corrected time-step method in the region of the equator,which tends to be in a more north–south direction and to have less coverage for the east–west ionospheric gradient.This study also addresses the limitations of temporal variation correction coverage and recommends using only the correction from self-observations.All processes are developed under simulations because observational data are still difficult to obtain.Our findings demonstrate that the corrected time-step method yieldsσvig values consistent with other approaches.

关键词： ground-based augmentation system nominal ionospheric gradient time-step correction global ionospheric model

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Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism

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Molecular Plant 2010年第1期3卷 125-142页

作者： Doris Albinsky Miyako Kusano Mieko Higuchi Naomi Hayashi Makoto Kobayashi Atsushi Fukushima Masaki Mori Takanari Ichikawa Keiko Matsui Hirofumi Kuroda Yoko Horii Yuko Tsumoto Hitoshi Sakakibara Hirohiko Hirochika Minami Matsui kazuki saito RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Inage-ku Chiba 263-8522 Japan

Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length （FL） cDNAs （rice FOX Arabidopsis lines） using a gas chromatography-time-of-flight mass spectrometry （GC-TOF/MS）-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candi- date lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Ara- bidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries （LOB） Domain （LBD）/ Asymmetric Leaves2-1ike （ASL） gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.

关键词： Metabolite profiling GC-TOF/MS rice FOX Arabidopsis lines transcript profiling LBD37/ASL39 nitrogenmetabolism.

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Education Programs for Invasive Procedures Involving Nurses: A Scoping Review

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Open Journal of Nursing 2024年第5期14卷 200-224页

作者： Hiromi Shibuya Akiko saito Masumi Mugiyama Noyuri Yamaji Chisato Eto Satoshi Shibuya Department of Nursing Faculty of Health Science Technology Bunkyo Gakuin University Tokyo Japan Department of Health Care Policy and Health Economics Institute of Medicine University of Tsukuba Ibaraki Japan Institute of Clinical Epidemiology Showa University Tokyo Japan Department of Family Nursing Division of Health Science and Nursing Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Global Health Nursing Graduate School of Nursing St. Luke&#8217s International University Tokyo Japan School of Nursing Tokyo Women&#8217s Medical University Tokyo Japan Department of Integrative Physiology School of Medicine Kyorin University Tokyo Japan

Background: Implementing invasive procedures is an important part of patient management by clinical nurses;however, there are gaps between nurses’ actual knowledge and skills and expected professional care capacity. Purpose: This scoping review aimed to map the existing literature related to recent institution-provided educational programs for invasive procedures involving clinical nurses. This study seeks to understand the contents of educational programs and the methods for assessing educational effects. Methods: This scoping review was completed using the following four databases: PubMed (MEDLINE), Embase, Cochrane Library, and Emcare for the period 2000-2022. We included studies that used all forms of educational approaches (e.g., didactic lectures, hands-on training, or on-the-job training). This scoping review considered peer-reviewed publications published in English using quantitative, qualitative, or mixed approaches. A total of 83 studies underwent in the final analysis. Results and Conclusion: A combination of didactic lectures and hands-on training was provided as an educational program in most studies. Contrary to our prediction, educational interventions with advanced technologies such as VR are extremely rare, suggesting that the effectiveness of advanced technologies in learning invasive procedures should be examined to facilitate and retain educational effects more efficiently in future studies. Regarding the assessment of educational effects, nurses’ cognitive (i.e., theoretical knowledge about procedures), psychomotor (i.e., implementing procedures), and psychological aspects (e.g., confidence and self-efficacy in procedures) were evaluated using questionnaires and observational methods. While most studies used a one-group pretest-posttest design, the ratio of randomized controlled trials (RCT) was relatively low. Thus, an RCT design should be introduced in future studies to test the validity of the developed educational program more accurately.

关键词： Training Education Invasive Procedure Nurses Assessment

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Identification of telomere maintenance gene variations related to lung adenocarcinoma risk by genome-wide association and whole genome sequencing analyses

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Cancer Communications 2024年第2期44卷 287-293页

作者： Kouya Shiraishi Atsushi Takahashi Yukihide Momozawa Yataro Daigo Syuzo Kaneko Takahisa Kawaguchi Hideo Kunitoh Shingo Matsumoto Hidehito Horinouchi Akiteru Goto Takayuki Honda Kimihiro Shimizu Masahiro Torasawa Daisuke Takayanagi Motonobu saito Akira saito Yuichiro Ohe Shun-ichi Watanabe Koichi Goto Masahiro Tsuboi Katsuya Tsuchihara Sadaaki Takata Tomomi Aoi Atsushi Takano Masashi Kobayashi Yohei Miyagi Kazumi Tanaka Hiroyuki Suzuki Daichi Maeda Takumi Yamaura Maiko Matsuda Yoko Shimada Takaaki Mizuno Hiromi Sakamoto Teruhiko Yoshida Yasushi Goto Tatsuya Yoshida Taiki Yamaji Makoto Sonobe Shinichi Toyooka Kazue Yoneda Katsuhiro Masago Fumihiro Tanaka Megumi Hara Nobuo Fuse Satoshi S.Nishizuka Noriko Motoi Norie Sawada Yuichiro Nishida kazuki Kumada Kenji Takeuchi Kozo Tanno Yasushi Yatabe Kuniko Sunami Tomoyuki Hishida Yasunari Miyazaki Hidemi Ito Mitsuhiro Amemiya Hirohiko Totsuka Haruhiko Nakayama Tomoyuki Yokose Kazuyoshi Ishigaki Toshiteru Nagashima Yoichi Ohtaki Kazuhiro Imai Ken Takasawa Yoshihiro Minamiya Kazuma Kobayashi Kenichi Okubo Kenji Wakai Atsushi Shimizu Masayuki Yamamoto Motoki Iwasaki Koichi Matsuda Johji Inazawa Yuichi Shiraishi Hiroyoshi Nishikawa Yoshinori Murakami Michiaki Kubo Fumihiko Matsuda Yoichiro Kamatani Ryuji Hamamoto Keitaro Matsuo Takashi Kohno Division of Genome Biology National Cancer Center Research InstituteTokyoJapan Department of Clinical Genomics National Cancer Center Research InstituteTokyoJapan Laboratory for Statistical Analysis Center for Integrative Medical SciencesRIKENYokohamaJapan Department of Genomic Medicine Research InstituteNational Cerebral and Cardiovascular CenterOsakaJapan Laboratory for Genotyping Development Center for Integrative Medical SciencesRIKENYokohamaJapan Center for Antibody and Vaccine Therapy Research HospitalInstitute of Medical ScienceThe University of TokyoTokyoJapan Department of Medical Oncology and Cancer Center and Center for Advanced Medicine against CancerShiga University of Medical ScienceOtsuJapan Division of Medical AI Research and Development National Cancer Center Research InstituteTokyoJapan Center for Genomic Medicine Graduate School of MedicineKyoto UniversityKyotoJapan Department of Medical Oncology Japanese Red Cross Medical CenterTokyoJapan Department of Thoracic Oncology National Cancer Center Hospital EastJapan Department of Thoracic Oncology National Cancer Center HospitalTokyoJapan Department of Cellular and Organ Pathology Graduate School of MedicineAkita UniversityAkitaJapan Department of Respiratory Medicine Tokyo Medical and Dental UniversityBunkyo-kuTokyoJapan Division of General Thoracic Surgery Department of SurgeryShinshu University School of MedicineMatsumotoNaganoJapan Department of General Surgical Science Division of General Thoracic SurgeryGunma University Graduate School of MedicineIntegrative Center of General SurgeryGunma University HospitalGunmaJapan Department of Gastrointestinal Tract Surgery Fukushima Medical University School of MedicineFukushimaJapan StaGen Co. LtdTokyoJapan Division of Thoracic Surgery National Cancer Center HospitalTokyoJapan Department of Thoracic Surgery National Cancer Center Hospital EastChibaJapan Division of Translational Informatics Exploratory Oncology Research and Clinical Trial Center(EPOC

Dear editor,Lung carcinoma is responsible for the highest fatal-ity rate among cancer-related deaths globally,with lung adenocarcinoma(LADC)emerging as the prevailing sub-type.

关键词： adenocarcinoma lung Lung

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The Role of Jasmonates as Antibulbing Substances in the Bulb Formation of Onion

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Agricultural Biotechnology 2024年第1期13卷 1-4,8页

作者： Noboru Takada Atsushi saito Yuuki Matsuzuka Tatsushi Mochiduki Eriko Wakita Meng WANG Yasunori Koda Faculty of Agriculture and Life Science Hirosaki University3Bunkyo-choHirosaki 036-8561Japan The United Graduate School of Agricultural Science Iwate UniversityMoriokaIwate 020-8550Japan National Institute of Vegitable and Tea Science National Agriculture and Food Research Organization360 KusawaAnoTsu 514-2392Japan Research Faculty of Agriculture Hokkaido UniversityKita-9 Nishi-9Kita-kuSapporo 060-8589Japan

Onion plants form spherical bulbs under long-day conditions.Substances regulating bulb formation remain unknown.In the course of chemical studies on the bulb formation,α-linolenic acid was isolated from onion extracts as an antibulbing substance,the amount of which was synchronized with the bulb formation.Since allene oxide synthase inhibitor canceled the antibulbing activity ofα-linolenic acid,it was disclosed that jasmonic acid concerns this regulation.Structure-activity-relationship study revealed that its(3R,7S)stereochemistry is necessary for showing its antibulbing activity.It is concluded that(3R,7S)-jasmonate derived fromα-linolenic acid actually participates in the regulation of bulb formation.

关键词： Onion(Allim cepa L.cv.Higuma) Isolation Bulb formation Antibulbing substance α-Linolenic acid Methyl jasmonate

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Integration of Metabolomics and Transcriptomics for Phytochemical Genomics in Arabidopsis and Non-model Plants

Integration of Metabolomics and Transcriptomics for Phytoche...

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The 1st International Conference on Plant Secondary Metabolism

作者： kazuki saito Chiba University Graduate School of Pharmaceutical Sciences Inage-ku Chiba 263-8522 Japan RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan

＜正＞The completion of the whole genome sequence ofArabidopsis thaliana has made it possible to discover the genes involved in metabolism in a high throughput manner by determining gene-to-metabolite correlation through the comprehensive analysis of metabolite accumulation and gene expression[1].In silico co-expression analysis of genes involved in flavonoid metabolism in Arabidopsis was performed using a publicly available transcriptome database of DNA microarrays.We inferred a co-expression framework model of the genes involved in the path-

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