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在分离静脉注射免疫球蛋白时病毒的灭活和消除:湿热处理和PEG分离

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微生物学免疫学进展 1992年第2期20卷 58-62页

作者： uemura Y 王立兰

静脉注射免疫球蛋白(IVIG)治疗无丙种球蛋白血症的效果和安全性,使治疗原发性血小板减少性紫癜、kawasaki病、骨髓移植和获得性免疫缺陷综合征都成为适应症。这些病人的防御能力可能很弱,需要给相对大剂量的IVIG。人类免疫缺陷病毒(HIV)

关键词：免疫球蛋白病毒灭活湿热处理

Screening serum hepatocellular carcinoma-associated proteins by SELDI-based protein spectrum analysis

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World Journal of Gastroenterology 2008年第8期14卷 1257-1262页

作者： Jie-Feng Cui Yin-Kun Liu Hai-Jun Zhou Xiao-Nan Kang Cheng Huang Yi-Feng He Zhao-You Tang Toshimasa uemura Liver Cancer Institute Zhongshan Hospital Fudan University Shanghai200032 China Nanotechnology Research Institute (NRI) National Institute of Advanced Industrial Science and Technology(AIST) Tsukuba Central-4 Tsukuba 3058562 Japan

AIM： To find out potential serum hepatocellular carcinoma （HCC）-associated proteins with low molecular weight and low abundance 13y SELDI-based serum protein spectra analysis, that will have much application in the diagnosis or differentiated diagnosis of HCC, as well as giving a better understanding of the mechanism of hepato-card nogenesis.METHODS： Total serum samples were collected with informed consent from 81 HCC patients with HBV（＋）/ cirrhosis（＋）, 36 cirrhosis patients and 43 chronic hepatitis B patients. Serum protein fingerprint profiles were first generated by selected WCX2 protein chip capture integrating with SELDI-TOF-MS, then normalized and aligned by Ciphergen SELDI Software 3.1.1 with Biomarker Wizard..Comparative analysis of the intensity of corresponding protein fingerprint peaks in normalized protein spectra, some protein peaks with significant difference between *** and cirrhosis or chronic hepatitis B were found.RESULTS： One hundred and twenty-eight serum protein peaks betweeri.2000 and 30000Da were identified under the condition of signal-to-noise 〉 5 and minimum threshold for cluster 〉 20%. Eighty-seven of these proteins were showed significant differences in intensity between HCC and cirrhosis （P 〈 0.05）. Of the above differential proteins, 45 proteins had changes greater than two-fold, including 15 upregulated proteins and 30 downregulated proteins i.n HCC serum. Between HCC and chronic hepatitis B, 9 of 52 differential proteins （P 〈 0.05） had intensities of more than two-fold, including 2 upregulated proteins and 7 downregulated proteins in HCC serum. Between cirrhosis and chronic hepatitis B, 28 of 79 significant differential proteins （P 〈 0.05） changes greater than two-fold in intensity, including 17 upregulated proteins and 11 downregulated proteins in cirrhosis serum. For the analysis of these leading differential proteins in subtraction difference mode among three diseases, the five common downregulated proteins in HCC serum （M/Z 2

关键词： Hepatocellular carcinoma SELDI-TOF-MS

Elucidation of neural circuit mechanism integrating noxious stimulus and ambient temperature sensation in Drosophila

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Elucidation of neural circuit mechanism integrating noxious ...

中国神经科学学会第十二届全国学术会议

作者： Kai Li Koun Onodera Risa Nishimura Akira Murakami Tadashi uemura Tadao Usui uemura Lab. Graduate School of Biostudies Kyoto University

Avoidance of harmful stimuli is essential for animals’ survival. Such a nociceptive response is a protective mechanism to escape from damage to the tissue. Previously, we are interested in Class IV dendritic arborization neurons(Class IV neurons) in Drosophila larvae, which are polymodal nociceptors responsible for thermal, mechanical and light sensation. The activation of these neurons leads to distinct behaviors, depending on the inputs. For example, the noxious thermal stimulation triggers a stereotyped avoidance response named rolling escape behavior. However, the downstream processing mechanism of harmful stimuli is still elusive. Interestingly, in our and other’s behavior tests, we found a group of thermosensory neurons in larval brain can also induce the rolling behavior, which indicated that there is a second input for the behavior(Luo et al., Nature Neurosci., 2016). Based on our behavior test, this central thermosensors might not only be the primary sensory neurons but also the Class IV neurons’ downstream interneurons. Why larva has such thermosensory neurons deep in the brain? How is this second input transduced to the downstream? How such descending input from brain affect the peripheral nociceptive sensation? How are two input integrated by the downstream circuits and contribute to the behavioral decision. We believe such integration of multiple inputs will bring the animal certain ecological benefits. The Drosophila larva is an excellent model system to study such structural and functional patterns of circuits and how such neural circuits make the behavioral selection. It has a relatively small size of nervous system with 00,000 neurons. Its transparent tissue makes it amenable to neuronal activation using optogenetic tools. Moreover, powerful genetic toolkit in Drosophila allow us to anatomically and functionally map circuits. By investigating these particular circuits in Drosophila, we expect that certain universal mechanism underlying integration o

关键词： Drosophila nociception avoidance behavior neural circuit

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田边尚雄与东洋音乐的概念

黄钟（武汉音乐学院学报） 2010年第2期 81-87页

作者：植村幸生[日] 周耘(译) 东京艺术大学 430060

作为20世纪日本民族音乐学的领袖人物,田边尚雄(Tanabe Hisao,1883-1984)从明治末期到战后,一直活跃在该领域的最前沿。本文聚焦于田边尚雄在1920年到1960年的著述中纳入研究对象的所谓"东洋音乐"的表述,旨在解读田边尚雄的"东洋音乐"... 详细信息

作为20世纪日本民族音乐学的领袖人物,田边尚雄(Tanabe Hisao,1883-1984)从明治末期到战后,一直活跃在该领域的最前沿。本文聚焦于田边尚雄在1920年到1960年的著述中纳入研究对象的所谓"东洋音乐"的表述,旨在解读田边尚雄的"东洋音乐"概念的实质,进而探讨现代日本人关于亚洲音乐之认识的原点。

关键词：田边尚雄东洋音乐大东亚音乐概念实质

Intravascular proliferating anaplastic lymphoma kinasepositive anaplastic large-cell lymphoma

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World Journal of Hematology 2015年第2期4卷 10-15页

作者： Kohei Shiroshita Jun-ichiro Kida Kensuke Matsumoto Makiko uemura Genji Yamaoka Yumi Miyai Reiji Haba Osamu Imataki Post-Graduate Clinical Training Center Kagawa University Hospital Division of Hematology and Stem Cell Transplantation Department of Internal MedicineFaculty of MedicineKagawa University Department of Laboratory Medicine Kagawa University Hospital Department of Diagnostic Pathology Faculty of MedicineKagawa University

An 82-year-old Japanese man visited our emergency unit complaining of dyspnea. Laboratory data showed 15% atypical lymphocytes in peripheral blood which expressed the T-cell phenotype. Chest/abdominal computed tomography depicted hepatosplenomegaly and swelling of systemic lymph nodes. The patient died of advanced respiratory failure 5 d after the first occurrence of his dyspnea. At autopsy, the pathological features revealed a diffuse infiltration of large atypical lymphocytes to systemic organs including the spleen and lung. In immunohistochemical staining, these cells expressed CD30, TIA-1, anaplastic lymphoma kinase(ALK), CD5 and CD3. An advanced surface molecule analysis revealed a lack of CD54(intercellular cell adhesion molecule-1) and CD56(neural cell adhesion molecule). We observed the proliferation and infiltration of these lymphoma cells specifically at the intravascular lesions similar to intravascular lymphoma(IVL). T-cell IVL is not established as an independent clinical entity in the World Health Organization classification, and our patient's ALK-positive T-IVL in lung appears to be the first reported case.

关键词： Malignant lymphoma Cytotoxic molecule Intravascular lymphoma Anaplastic lymphoma kinase Anaplastic large-cell lymphoma

Study on viscosity of Hydoroxypropylmethylcellulose stearoxy ether(SANGELOSE) under mechanical shearing

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Study on viscosity of Hydoroxypropylmethylcellulose stearoxy...

Down-Regulation of Claudin-1 Expression in Gastric Cancer Mucosa Is Correlated with Poor Prognosis

生物颗粒与粉体制备、应用技术研讨会

作者：稻本靖也 Toshio Shimamoto Shunji Uramatsu Shinichi Akiyama Kozo Kishi Toshinobu uemura 大同化成工业株式会社

Purpose:The clarification of viscosity characteristics of aqueous polymer gel under mechanical shearing is important for formulation design of gel and emulsion dosage forms in cosmetic and pharmaceutical *** study was focused on the clarification of viscosity change of aqueous SGL gel by an agitating homogenizer and a ***: Homogenizer as a medium mechanical shearing machine and microfluidizer as an extremely mechanical high shearing one were used,*** viscosity of SGL gel was investigated by treating with respective shearing,that is revolution speed /operation time in homogenizer's case and operation pressure/ passing time in case of microfluidizer's one. Carboxyvinylpolymer was used for ***:It was found that the viscosity of SGL gel was stable under medium mechanical shearing by a *** viscosity of SGL gel under extremely high shearing by a microfluidizer was decreased,however,the decrease level of viscosity might be tolerable to prepare emulsion dosage *** the other hand,CVP gel is unstable in viscosity under medium and high shearing.

关键词： Hydoroxypropylmethylcellulose stearoxy ether SANGELOSE viscosity mechanical shearing nano-emulsion

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Open Journal of Pathology 2014年第4期4卷 206-212页

作者： Eiichiro Hirakawa Yasunori Tokuhara Tatsuya Morinishi Hiroyuki Ohsaki Erika uemura Yukari Miki Toru Matsunaga Yoshio Kushida Reiji Haba Laboratory of Pathology Department of Medical Technology Kagawa Prefectural University of Health Sciences Kagawa Japan Department of Medical Technology Ehime Prefectural University of Health Sciences Ehime Japan Department of Diagnostic Pathology Kagawa University Hospital Kagawa Japan Department of Medical Hygiene Technology Course Kochi Gakuen College Kochi Japan

Background: Cell adhesion molecule abnormalities are given as one reason for the occurrence of invasion and metastasis in various cancers. In this study, we conducted an immunohistochemical examination of cell adhesion molecule claudin-1 in mucosa and invasive front of gastric cancer, and investigated the correlation of claudin-1 expression and clinicopathological factors. Methods: Immunohistochemical examination was performed for 35 patients who underwent surgery be-tween January 2010 and October 2011, to assess the correlation of claudin-1 with primary gastric cancer. Results: The expression rate of claudin-1 was 48.6% (17/35) in 35 gastric carcinoma patients. The positive rates of claudin-1 were 42.9% (15/35) in mucosa and 28.6% (10/35) in invasive front of gastric cancer. The expression rate of claudin-1 in invasive front was lower than in mucosa. From comparing claudin-1 expression in mucosa, it was found that well-to moderately-differentiated adenocarcinoma had significantly more claudin-1 expression than poorly-differrentiated adenocarcinoma. Claudin-1 expression of well-to moderately-differentiated adenocar-cinoma decreased in the invasive front of gastric cancer. Expression of claudin-1 in mucosa was negative in many cases with advanced tumor invasion (T2, T3, T4) and positive in many cases with early tumor invasion (T1), with a significant difference between the two

关键词： Claudin-1 Immunohistochemistry Mucosa Invasive Front Gastric Cancer

A case of severe acalculous cholecystitis associated with sorafenib treatment for advanced hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2012年第5期4卷 115-118页

作者： Yosuke Aihara Hitoshi Yoshiji Masaharu Yamazaki Yasuhide Ikenaka Ryuichi Noguchi Chie Morioka Kosuke Kaji Haruki Tastumi Keisuke Nakanishi Maiko Nakamura Junichi Yamao Masahisa Toyohara Akira Mitoro Masayoshi Sawai Motoyuki Yoshida Masao Fujimoto Masahito uemura Hiroshi Fukui Third Department of Internal Medicine Nara Medical UniversityNara 634-8522Japan

Sorafenib,a multikinase inhibitor,is the first and only drug,which improves significantly the overall survival in patients with advanced hepatocellular carcinoma(HCC).However,many patients experience diverse side effects,some of them severe and *** date,acute acalculous cholecystitis has not been documented in association with a HCC patient treated with ***,we report the case of a 43-yearold woman with hepatitis C virus-related advanced *** received sorafenib,and later complained ofa sudden onset of severe right hypocondrial pain with rebound tenderness and muscle *** examination showed mild elevation of transaminases,biliary enzymes,bilirubin,inflammation markers,and a marked peripheral *** computed tomography(CT) revealed a swollen gallbladder with exudate associated with severe inflammation without stones or ***,sorafenib treatment was stopped immediately,and steroid-pulse therapy was *** therapy drastically improved all clinical manifestations along with normalization of CT findings,eosinophilia,and liver *** summary,we herein report a rare case of acute severe acalculous cholecystitis associated with sorafenib in the patient with advanced HCC.

关键词： Hepatocelluar carcinoma Sorafenib Acalculous cholecystitis

Human CD4^- CD8^- Invariant Natural Killer T Cells Promote IgG Secretion from B Cells Stimulated by Cross-Linking of Their Antigen Receptors

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World Journal of Vaccines 2016年第2期6卷 34-41页

作者： Tomomitsu Miyasaka Yurie Watanabe Yukiko Akahori Namiko Miyamura Keiko Ishii Yuki Kinjo Yoshitsugu Miyazaki Tian-Yi Liu Yasushi uemura Kazuyoshi Kawakami Department of Medical Microbiology Mycology and Immunology Tohoku University Graduate School of Medicine Sendai Japan Department of Chemotherapy and Mycoses National Institute of Infectious Diseases Tokyo Japan Division of Immunology Aichi Cancer Center Research Institute (ACCRI) Nagoya Japan Key Laboratory of Cancer Center Chinese PLA General Hospital Beijing China Division of Cancer Immunotherapy Exploratory Oncology Research & Clinical Trial Center National Cancer Center (NCC) Kashiwa Japan

Immunoglobulin (Ig) M production can be induced by the interaction of thymus-independent type-2 (TI-2) antigen (Ag) with B cell Ag receptors (BCRs) without the involvement of conventional T cells;for IgG production through the same process, however, a second signal is required. Previous studies have reported that invariant natural killer T (iNKT) cells may be responsible for the second signal involved in IgG production. In the present study, we addressed whether human iNKT cells could participate in the production of Ig against TI-2 Ag in vitro. Two major distinct subsets of human iNKT cells, CD4+ CD8β- (CD4) and CD4- CD8β- [double negative (DN)] cells, were generated from peripheral blood monocytes from a healthy volunteer. BCR engagement, triggered by anti-IgM antibody stimulation, examined here as a model of BCR engagement triggered by TI-2 Ag, induced abundant IgM production by B cells. Both CD4 and DN iNKT cells reduced IgM production and conversely enhanced IgG production in a dose-dependent manner. In addition, IgG production by CD19+CD27- (naïve) and CD19+CD27+ (memory) B cells was predominantly promoted by DNiNKT cells rather than CD4 iNKT cells;nevertheless, IgM production by both B cell subsets was similarly reduced by either subset of iNKT cells. These results suggest that the DN iNKT subsets may preferentially promote Ig class switching by B cells upon stimulation with TI-2 Ag.

关键词： Invariant Natural Killer T Cells TI-2 Antigen B Cells IgM IgG