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Multimodal label-free microscopy

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Journal of Innovative Optical Health Sciences 2014年第5期7卷 17-38页

作者： Nicolas Pavillon katsumasa Fujita Nicholas Isaac Smith Biophotonics LaboratoryImmunology Frontier Research Center(IFReC Department of Applied Physics Osaka UniversitySuitaOsaka 565-0871Japan

This paper reviews the different multimodal applications based on a large ext ent of label-freeinaging modalities,ranging from linear to nonlinear optics,while also inchuding *** put specific emphasis on multimodal measurements going across the usual boundaries between imaging modalities,whereas most multimodal platforms combine techniquesbased on similar light interactions or similar hardware *** this review,we limitthe scope to focus on applications for biology such as live cells or tissues,since by their nat ure ofbeing alive or fragile,we are often not free to take liberties with the image acquisition times andare forced to gather the maximum amount of information possible at one *** such samples,imaging by a given label-free method usually presents a challenge in obt aining suficient opticalsignal or is limited in terms of the types of observable *** imaging is thenparticularly attractive for these samples in order to maximize the amount of measured *** multimodal imaging is always useful in the sense of acquiring additional infor-mation from additional modes,at times it is possible to attain information that could not bediscovered using any single mode alone,which is the essence of the progress that is possible usinga multimodal approach.

关键词： Optical microscopy nonlinear optics microspectroscopy

Estimating Individual Tree Diameter and Stem Volume Using Airborne LiDAR in Saga Prefecture, Japan

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Open Journal of Forestry 2018年第2期8卷 205-228页

作者： katsumasa Oono Satoshi Tsuyuki Department of Global Agricultural Sciences The University of Tokyo Tokyo Japan Forest & Agriculture Solution Department Asia Air Survey CO. LTD. Kanagawa Japan

Advancements in airborne LiDAR analysis technology have made it possible to quantify forest resource volumes based on individual trees, and such technology may soon replace field surveys. Unlike individual tree detection or tree height measurements, diameter at breast height (DBH) is difficult to determine directly from measured data and is instead estimated indirectly using the correlation between crown size and DBH. Indicators that represent crown size include crown area, surface area, length, and length ratio, and were utilized with tree height as explanatory variables in ten combinations to determine a regression formula. DBH and tree height calculated from the regression formula were applied to an equation to calculate stem volumes of individual trees. Airborne LiDAR measurements were taken using ALS50-II and ALS60 (Leica) at a density of 4 points/m2. An evaluation of the relationship between the regression formulae and DBH estimates indicated that a combination of crown area, tree height, and crown ratio for Japanese cedar, and a combination of crown area and tree height for Japanese cypress, yielded the highest coefficients of determination. The average error and RMSE were 6.9% and 2.38 cm respectively for Japanese cedar, while the corresponding values for Japanese cypress were 8.35% and 2.51 cm. Once the relationship was extended to the stem volumes of individual trees, the average error was 14.4% and RMSE was 0.10 m3 for Japanese cedar. The corresponding values for Japanese cypress were 18.9% and 0.10 m3. These results demonstrate the potential use of airborne LiDAR as a substitute for field surveys.

关键词： Airborne LiDAR Diameter at Breast Height Individual Treevolume Forest Resource Individual-Tree-Based Approach

Dual Visual Cryptography Using the Interference Color of Birefringent Material

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Journal of Software Engineering and Applications 2017年第8期10卷 754-763页

作者： Huangyi Qin Toshiki Matsusaki Yusuke momoi Kenji Harada Department of Computer Science Kitami Institute of Technology Kitami Japan

Visual cryptography is a method of encrypting an image into several encrypted images. Conventional visual cryptography can display only monochrome images. We previously proposed a color visual cryptography method that uses the interference color of high-order retarder films and encrypts one secret image into two encrypted images. In other words, this method can only encrypt one image at a time. In this paper, we propose a new method that encrypts two color images using interference color.

关键词： Visual Cryptography Polarizer Interference Color Birefringent Material

Neural Activation by Milnacipran and Memory Extinction

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Journal of Behavioral and Brain Science 2012年第2期2卷 141-145页

作者： Hisahito Ishida Masaaki Iwata Yukihiko Shirayama katsumasa Muneoka Department of Anatomy I Showa University School of Medicine Tokyo Japan Department of Neuropsychiatry Faculty of Medicine Tottori University Tottori Japan Department of Psychiatry Teikyo University Chiba Medical Center Chiba Japan

Background: Among neurotransmitter influencing memory formation, the noradrenergic system has been recognized as an important system. Memory formation involves various regions including the prefrontal cortex, hippocampus, amygdala and septum. Method: We investigated the effects of milnacipran on passive avoidance task and evaluated Fos counting in the prefrontal cortex, hippocampus, septum, amygdala and nucleus accumbens. Results: The milnacipran-treated rats (20 mg/kg, 4 days) showed a significant decrease in the number of Fos-immunoreactive cells in the infralimbic portion of prefrontal cortex, the shell portion of nucleus accumbens and the CA1 region of hippocampus, but a significant increase in the Fos counts in the lateral septum with no changes in the Fos counts in the striatum and amygdala. The milnacipran-treated rats showed amelioration in memory extinction (although not statistically significant), but not in memory acquisition and consolidation in the passive avoidance test. Conclusion: The differential activation of the brain regions might be possible sites for ameliorating memory extinction as well as antidepressant effects.

关键词： Milnacipran Passive Avoidance Task Prefrontal Cortex Nucleus Accumbens Hippocampus Septum

Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第11期19卷 844-852页

作者： Toshinori YOSHIHARA Takao SUGIURA Nobuyuki MIYAJI Yuki YAMAMOTO Tsubasa SHIBAGUCHI Ryo KAKIGI Hisashi NAITO katsumasa GOTO Daijiro OHMORI Toshitada YOSHIOKA Graduate School of Health and Sports Science Juntendo UniversityInzaiChiba 270-1695Japan Faculty of Education Yamaguchi UniversityYamaguchiYamaguchi 753-8513Japan Toyo Koso Kagaku Co.Ltd. UrayasuChiba 279-0041Japan Institute of Health and Sports Science Kurume UniversityKurumeFukuoka 839-8502Japan Institute of Liberal Arts and Science Kanazawa UniversityKanazawaIshikawa 920-1192Japan Faculty of Medicine Juntendo UniversityBunkyo-kuTokyo 113-0033Japan Graduate School of Health Sciences Toyohashi SOZO UniversityToyohashiAichi 440-8511Japan Department of Chemistry School of MedicineJuntendo UniversityInzaiChiba 270-1695Japan Hirosaki Gakuin University HirosakiAomori 036-8577Japan

We examined the effect of a combination of astaxanthin （AX） supplementation, repeated heat stress, and intermittent reloading （IR） on satellite cells in unloaded rat soleus muscles. Forty-nine male Wistar rats （8-week-old） were divided into control, hind-limb unweighting （HU）, IR during HU, IR with AX supplementation, IR with repeated heat stress （41.0-41.5 ℃ for 30 min）, and IR with AX supplementation and repeated heat stress groups. After the experimental period, the antigravitational soleus muscle was analyzed using an immunohistochemical technique. Our results revealed that the combination of dietary AX supplementation and heat stress resulted in protection against disuse muscle atrophy in the soteus muscle. This protective effect may be partially due to a higher satellite cell number in the atrophied soleus muscle in the IR/AX/heat stress group compared with the numbers found in the other groups. We concluded that the combination treatment with dietary AX supplementation and repeated heat stress attenuates soleus muscle atrophy, in part by increasing the number of satellite cells.

关键词： Antioxidant astaxanthin Disuse muscle atrophy Heat stress Satellite cell

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Follow-up of coronary artery lesions caused by Kawasaki disease and the value of coronary angiography

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Chinese Medical Journal 2002年第5期115卷 681-684,148页

作者：龚方戚白石裕比湖桃井真里子浙江大学医学院附属儿童医院浙江310003 Department of Pediatrics Jichi Medical schoolMinamikawachi

OBJECTIVE: To investigate the course of coronary artery lesions caused by Kawasaki disease, and the value of coronary angiography (CAG) and two-dimensional echocardiography (2-D Echo) in the evaluation and follow-up of coronary artery lesions. METHODS: Eighty seven patients with coronary artery lesions caused by Kawasaki disease from 1979 to 1997 were retrospectively analyzed. One hundred and sixty-seven CAGs were performed in 87 patients during follow-up. CAG was repeated every 1-3 years in each patient until complete regression was confirmed. 2-D Echo was performed before CAG each time. The longest period of follow-up was 16 years and 6 months. Patients were treated with aspirin or aspirin and warfarin. RESULTS: During follow-up, the coronary artery lesions regressed in 48/87 (55%) patients, however, they developed into severe coronary artery lesions in 6/87 (7%) patients in whom coronary artery bypass surgery was performed. The coronary artery aneurysm regressed in some patients, while stenotic lesions remained or developed. The ratio of coronary artery stenotic lesions to aneurysms increased progressively. This study showed that Echo diagnosis of coronary artery lesions has 'false positives' and 'false negatives'. Only 76% of coronary aneurysms and 18% of stenotic lesions could be found by 2-D Echo. No stenotic lesion could be found in distal segments of the coronary artery. CONCLUSIONS: Long term follow up revealed spontaneous regression occurred in 55% of patients and development into severe coronary artery stenosis in 7%. It is necessary to perform long-term follow-up in patients with coronary artery lesions caused by Kawasaki disease. 2-D Echo can not completely replace CAG during follow-up of coronary artery lesions caused by Kawasaki disease.

关键词： Adolescent Child Child, Preschool Coronary Aneurysm Coronary Angiography Coronary Vessels Disease Progression Female Follow-Up Studies Humans Infant Male Mucocutaneous Lymph Node Syndrome Retrospective Studies Time Factors

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SHIROIYA酒店

现代装饰 2021年第3期 120-127页

作者： Sou Fujimoto katsumasa Tanaka(摄影) Shinya Kigure(摄影) 舒禹(编译) Sou Fujimoto Architects

该项目旨在翻修前桥市中心的一栋被称为"遗产塔"的四层钢筋混凝土主楼建筑。设计师的第二项任务是建造新的大楼,称为"绿塔"。自江户时代以来,主楼内的"白屋旅馆"已有300多年的历史,旅馆关闭后使得这座建筑空置。设计师在考察现场后,决... 详细信息

该项目旨在翻修前桥市中心的一栋被称为"遗产塔"的四层钢筋混凝土主楼建筑。设计师的第二项任务是建造新的大楼,称为"绿塔"。自江户时代以来,主楼内的"白屋旅馆"已有300多年的历史,旅馆关闭后使得这座建筑空置。设计师在考察现场后,决定拆除四层楼的墙壁与地板,创建一个巨大的中庭。

关键词：主楼建筑钢筋混凝土中庭江户时代酒店旅馆设计师地板

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痉挛患者拮抗肌收缩时缺乏Ib抑制调节

世界核心医学期刊文摘(神经病学分册) 2006年第12期 11-12页

作者： Morita H. Shindo M. momoi H. 郭俊 Shinshu University School of Medicine Matsumoto Asahi3-1-1 Japan Dr.Department of Medicine(Neurology) Clinical Neurophysiology Laboratory 3908621

OBJECTIVE:To examine the modulation of non-reciprocal group I(Ib)inhibition during tonic contraction of antagonist muscles in patients with spasticity vs normal ***:The authors studied 10 patients with spastic paraplegia due to cervical compression myelopathy and 16 age-matched normal *** inhibition to soleus motoneurons was recorded as the change in size of the H-reflex of the soleus,evoked by conditioning stimulus to the nerve innervating the medial gastrocnemius *** extent of inhibition was studied at rest and during tonic contraction of the pretibial muscles of variable ***:In the resting state,the extent of inhibition in the patients did not differ from normal *** antagonist contraction,the extent of inhibition increased both in the normal subjects and *** increment was smaller in the patients,especially in those with severe spastic *** smaller increment in the inhibition was correlated with the time required to walk 10 m in the ***:The authors observed a lack of modulation of Ib inhibition during tonic antagonist contraction in patients with spasticity,especially those with gait *** central modulation of non-reciprocal(Ib)interneurons may be responsible for spasticity.

关键词：拮抗肌患者 Ib

The Effect of Treatment Position on Rectal and Bladder Dose-Volume Histograms for Prostate Radiotherapy Planned with 3-Dimensional Conformal Radiotherapy, Intensity-Modulated Radiotherapy and Volumetric Modulated Arc Therapy

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第2期3卷 88-97页

作者： Kotaro Terashima katsumasa Nakamura Tomonari Sasaki Saiji Ohga Tadamasa Yoshitake Kazushige Atsumi Makoto Shinoto Kaori Asai Keiji Matsumoto Hidenari Hirata Yoshiyuki Shioyama Akihiro Nishie Hiroshi Honda Department of Clinical Radiology Graduate School of Medical Sciences Kyushu University Fukuoka Japan Department of Radiology Kyushu University Hospital at Beppu Beppu Japan Kyushu International Heavy Ion Beam Therapy Center Tosu Saga Japan

Purpose: To compare target coverage and organ at risk (OAR) sparing in the supine and prone positions with 3-dimensional conformal radiotherapy (3DCRT), intensity-modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) in low- and high-risk prostate radiotherapy cases. Materials and Methods: Using magnetic resonance images of five healthy volunteers, six treatment plans (supine 3DCRT, prone 3DCRT, supine IMRT, prone IMRT, supine VMAT and prone VMAT) were generated. Planning target volume 1 (PTV1) was defined as the prostate gland plus the seminal vesicles with adequate margins in a high-risk setting, while PTV2 was defined as prostate only with margins in a low-risk setting. The mean dose for both PTV1 and PTV2 was set at 78 Gy. Plans generated by each of the 3 techniques were compared between the supine and prone positions using dose-volume histograms (DVHs). Results: For PTV1, prone 3DCRT provided a significantly higher D98% than did supine 3DCRT, and its homogeneity index (HI) was significantly better. IMRT and VMAT values did not differ significantly between the prone and supine positions. For PTV2, no values differed significantly between the supine and prone positions under any treatment plan. With respect to OAR, the rectal D mean, D2%, V50, and V60 values of PTV1 were statistically higher in supine 3DCRT than in prone 3DCRT, while there were no significant differences in rectal values between the supine and prone positions with IMRT or VMAT. The rectal Dmean, V50, V60, V70, and V75 values of prone 3DCRT were significantly higher than those of supine IMRT or supine VMAT. There were no significant differences in any values for the rectum and bladder for PTV2. Conclusion: Although prone 3DCRT was found to be superior to supine 3DCRT in terms of rectal sparing in high-risk prostate cancer, IMRT and VMAT techniques could possibly cover this disadvantage.

关键词： Prostate Radiotherapy Dosimetric Comparison 3DCRT IMRT VMAT