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Journal of Pharmaceutical Analysis 2022年第1期12卷 186-191页

作者： karol jaroch Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooN2L 3G1Canada Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Toruń Poland85090BydgoszczPoland

The solid-phase microextraction technique quantifies analytes without considerably affecting the sample ***,a proof-of-concept study was conducted to demonstrate the use of coated probe electrospray ionization(coated-PESI)and coated blade spray(CBS)as ambient mass spectrometry approaches for monitoring drug *** ability of these methods was investigated for monitoring the dephosphorylation of a prodrug,combretastatin A4 phosphate(CA4P),into its active form,combretastatin A4(CA4),in a cell culture medium supplemented with fetal bovine *** CBS spot analysis was modified to achieve the same extraction efficiency as protein precipitation and obtained results in 7 *** coated-PESI performs extraction without consuming any samples,it is the preferred technique in the case of a limited sample *** coated-PESI only extracts small quantities of analytes,it uses the desorption solvent volume of 5-10 pL,resulting in high sensitivity,thus allowing the detection of compounds after only 1 min of *** biotransformation of CA4P into CA4 via phosphatases occurs within the simple matrix,and the proposed sample preparation techniques are suitable for monitoring the biotransformation.

关键词： Solid-phase microextraction Coated probe electrospray ionization Coated blade spray Combretastatin Biotransformation Prodrug activation

Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit

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Journal of Pharmaceutical Analysis 2023年第1期13卷 55-62页

作者： Runshan Will Jiang karol jaroch Janusz Pawliszyn Department of Chemistry University of WaterlooWaterlooN2L 3G1Canada Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in TorunBydgoszcz85-089Poland

Improved analytical methods for the metabolomic profiling of tissue samples are constantly ***,conventional sample preparation methods often involve tissue biopsy and/or homogenization,which disrupts the endogenous *** this study,solid-phase microextraction(SPME)fibers were used to monitor changes in endogenous compounds in homogenized and intact ovine lung *** SPME,a Biocrates AbsoluteIDQ assay was applied to make a downstream targeted metabolomics analysis and confirm the advantages of in vivo SPME *** AbsoluteIDQ kit enabled the targeted analysis of over 100 metabolites via solid-liquid extraction and *** analysis revealed significant differences between conventional liquid extractions from homogenized tissue and SPME results for both homogenized and intact tissue *** addition,principal component analysis revealed separated clustering among all the three sample groups,indicating changes in the metabolome due to tissue homogenization and the chosen sample preparation ***,clear differences in free metabolites were observed when extractions were performed on the intact and homogenized tissue using identical SPME ***,a direct comparison showed that 47 statistically distinct metabolites were detected between the homogenized and intact lung tissue samples(P<0.05)using mixed-mode SPME *** changes were probably due to the disruptive homogenization of the *** study's findings highlight both the importance of sample preparation in tissue-based metabolomics studies and SPME's unique ability to perform minimally invasive extractions without tissue biopsy or homogenization while providing broad metabolite coverage.

关键词： Solid-phase microextraction Solvent extraction Metabolomics Sample preparation In vivo sampling

One extraction tool for in vitro-in vivo extrapolation?SPME-based metabolomics of in vitro 2D,3D,and in vivo mouse melanoma models

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Journal of Pharmaceutical Analysis 2021年第5期11卷 667-674页

作者： karol jaroch Paulina Taczynska Marta Czechowska Joanna Bogusiewicz KamilŁuczykowski Katarzyna Burlikowska Barbara Bojko Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Torun Poland85-089BydgoszczPoland

Solid phase microextraction(SPME)in combination with high-resolution mass spectrometry was employed for the determination of metabolomic profile of mouse melanoma growth within in vitro 2D,in vitro 3D,and in vivo *** multi-model approach had never been investigated *** to the low-invasiveness of SPME,it was possible to perform time-course analysis,which allowed building time profile of biochemical reactions in the studied *** approach does not require the multiplication of samples as subsequent analyses are performed from the very same cell culture or from the same *** already reduces the number of animals required for experiment;therefore,it is with good concordance with the 3Rs rule(replacement,reduction,and refinement).Among tested models,the largest number of compounds was found within the in vitro 2D cell culture model,while in vivo and in vitro 3D models had the lowest number of detected *** results may be connected with a higher metabolic rate,as well as lower integrity of the in vitro 2D model compared to the in vitro 3D model resulting in a lower number of compounds released into medium in the latter *** terms of in vitro-in vivo extrapolation,the in vitro 2D model performed more similar to in vivo model compared to in vitro 3D model;however,it might have been due to the fact that only compounds secreted to medium were ***,in further experiments to obtain full metabolome information,the intraspheroidal assessment or spheroid dissociation would be necessary.

关键词： Solid-phase microextraction In vitro-in vivo extrapolation Metabolomics Melanoma B16F0 C57BL6 mouse model

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利用大数据提高机器人的能力

科技纵览 2024年第2期 44-48,4页

作者： Sergey Levine karol Hausman 不详

一系列机器人,一系列任务。在现实世界中变得普遍有用之前,机器人还有很多东西要学。虽然过去几年里,人工智能通过互联网这个庞大数据集的训练取得了令人印象深刻的进步,但机器人还没有类似的大型物理数据集可以利用。我们与世界各地的... 详细信息

一系列机器人,一系列任务。在现实世界中变得普遍有用之前,机器人还有很多东西要学。虽然过去几年里,人工智能通过互联网这个庞大数据集的训练取得了令人印象深刻的进步,但机器人还没有类似的大型物理数据集可以利用。我们与世界各地的其他32家机器人实验室合作,创建了现有最大的真实机器人动作开源数据集,对22种机器人进行了近100万次机器人试验,其中包含500种不同的技能以及与数干个不同对象的交互,包括图中的示例。

关键词：机器人人工智能物理数据大数据互联网数据集开源数据交互

Revisiting the standards of cancer detection and therapy alongside their comparison to modern methods

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World Journal of Methodology 2024年第2期14卷 17-37页

作者： Piotr Gromek Zuzanna Senkowska Elzbieta Pluciennik Zbigniew Pasieka Lin-Yong Zhao Adrianna Gielecinska Mateusz Kciuk karol Klosinski Zaneta Kaluzinska-Kolat Damian Kolat Department of Functional Genomics Medical University of LodzLodz 90-752LodzkiePoland Department of Biomedicine and Experimental Surgery Medical University of LodzLodz 90-136LodzkiePoland Department of General Surgery&Laboratory of Gastric Cancer State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Gastric Cancer Center West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Molecular Biotechnology and Genetics University of LodzLodz 90-237LodzkiePoland Doctoral School of Exact and Natural Sciences University of LodzLodz 90-237LodzkiePoland

In accordance with the World Health Organization data,cancer remains at the forefront of fatal *** upward trend in cancer incidence and mortality has been observed globally,emphasizing that efforts in developing detection and treatment methods should *** diagnostic path typically begins with learning the medical history of a patient;this is followed by basic blood tests and imaging tests to indicate where cancer may be located to schedule a needle *** initiation of diagnosis is crucial since delayed cancer detection entails higher costs of treatment and ***,there is a need for novel cancer detection methods such as liquid biopsy,elastography,synthetic biosensors,fluorescence imaging,and reflectance confocal *** therapeutic methods,although still common in clinical practice,pose many limitations and are ***,there is a dynamic advancement of clinical research and the development of more precise and effective methods such as oncolytic virotherapy,exosome-based therapy,nanotechnology,dendritic cells,chimeric antigen receptors,immune checkpoint inhibitors,natural product-based therapy,tumor-treating fields,and photodynamic *** present paper compares available data on conventional and modern methods of cancer detection and therapy to facilitate an understanding of this rapidly advancing field and its future *** evidenced,modern methods are not without drawbacks;there is still a need to develop new detection strategies and therapeutic approaches to improve sensitivity,specificity,safety,and ***,an appropriate route has been taken,as confirmed by the approval of some modern methods by the Food and Drug Administration.

关键词： Cancer detection Liquid biopsy Synthetic biosensors Fluorescence imaging Reflectance confocal microscopy Elastography Cancer therapy Tumor-treating fields Oncolytic virotherapy Nanotechnology

Mathematical Modelling of the Transient Response of Pipeline

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Journal of Thermal Science 2016年第6期25卷 549-557页

作者： Dawid Taler karol Kaczmarski Cracow University of Technology Faculty of Environmental Engineering Cracow University of Technology Faculty of Mechanical Engineering

Steam pipelines applied in power units operate at high pressures and *** addition,to stress from the pipeline pressure also arise high thermal stresses in transient states such as start-up,shutdown or a load change of the power ***-varying stresses are often the cause of the occurrence of fatigue cracks since the plastic deformations appear at the stress concentration *** determine the transient temperature of the steam along the steam flow path and axisymmetric temperature distribution in the pipeline wall,a numerical model of pipeline heating was *** determine the transient temperature of the steam and pipeline wall the finite volume method(FVM) was used Writing the energy conservation equations for control areas around all the nodes gives a system of ordinary differential equations with respect to *** system of ordinary differential equations of the first order was solved by the Runge-Kutta method of the fourth order to give the time-temperature changes at the nodes lying in the area of the wall and *** steam pressure distribution along pipeline was determined from the solution of the momentum conservation *** on the calculated temperature distribution,thermal stresses were *** friction factor was calculated using the correlations of Churchill and Haaland,which were proposed for pipes with a rough inner *** assess the accuracy of the proposed model,numerical calculations were also performed for the thin-walled pipe,and the results were compared to the exact analytical *** of the results shows that the accuracy of the proposed model of pipeline heating is very *** paper presents examples of the determination of the transient temperature of the steam and the wall.

关键词： pipeline steam heating walled satisfactory Pipeline conservation lying Kutta Runge

Influence of Biomass Co-combustion on Heating Surfaces Thermal Efficiency

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Journal of Thermal Science 2018年第5期27卷 427-432页

作者： GRADZIEL Slawomir WITKOWSKI karol Institute of Thermal Power Engineering Cracow University of Technology31-864 Kraków Poland European Institute for Energy Research 76131 Karlsruhe Germany

Pulverized coal-fired(PCF) boilers were first and foremost intended to fire pulverized hard or brown coal. However, biomass co-firing has become a fairly common practice in the Polish power generation system and many existing boilers have been modernized to serve this purpose. This paper presents calculations of the coefficient of thermal efficiency of the boiler heating surfaces and of the time needed for complete reconstruction of deposits on the second-stage steam reheater(RHII) of an OP-380 boiler with the output of 380×10~3 kg/h. The boiler was equipped with a purpose-designed installation of direct feeding of biomass. The main co-fired fuels were wood and sunflower husk pellets. Intense formation of deposits on the steam reheater tubes and problems related to a reduction in the diameters of the tubes were identified during the power unit operation.

关键词： biomass co-combustion ash deposit coefficient of thermal efficiency

Uniformity of Direct Unions of Chord

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Acta Mathematicae Applicatae Sinica 2015年第1期31卷 225-234页

作者： Rafa Kapelko karol Marchwicki Department of Computer Science Faculty of Fundamental Problems of Technology Wrocaw University of Technology

In this article we investigate the uniformity of direct union of k copies of Chord which is an improvedversion of Chord P2P system. We are interested in the maximal and the minimal areas controlled by nodes inthe system. We recall that the function n→k/k!.n-（1＋1/k）is a lower threshold and that the function n→1/n In（nlnk-k（n（k-1）1）/（k-1）1is an upper threshold for sizes of areas controlled by nodes in the direct union of kcopies of Chord.

关键词： peer-to-peer network modification of chord threshold asymptotic order statistic

Conditional Probability on the Kpka's D-posets

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Acta Mathematica Sinica,English Series 2012年第11期28卷 2197-2204页

作者： karol SAMUELCÍK Ivana HOLLÁ Department of Mathematics Faculty of Natural SciencesMatej Bel University

Kopka＇s D-poset is a very important notion in quantum structures. In this paper the conditional probability on the Kopka＇s D-posets is studied. The notion of conditional probability is introduced and the basic prope... 详细信息

关键词： Kopka's D-posers conditional probability on the Kopka's D-posers

The development of the Rook's(Corvus frugilegus)colony in the face of shifting spring phenology

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Avian Research 2022年第2期13卷 170-174页

作者： Adam Zbyryt karol Zub Cezary Mitrus Doctoral School of Exact and Natural Sciences University of Bialystok Mammal Research Institute Polish Academy of Sciences Department of Vertebrate Ecology and Paleontology Institute of BiologyWroclaw University of Environmental and Life Sciences

Many birds species breed in *** of the species in the Corvidae family nesting in groups is the Rook(Corvus frugilegus).The construction of the nest is costly for birds and needs high energy ***,birds should optimize the time of nest building in relation to environmental,especially weather,***,birds should adapt their breeding phenology,including the date of starting the construction of nests for climate *** observed the dynamics of increasing numbers of nests in a colony of Rooks in relation to air temperature,wind,rainfall,snowfall and day-length(indirect indicator of photoperiodism).Observations were carried out during three breeding seasons(2015-2017)in a large rookery in a big city in north-eastern *** increase in the number of nests was correlated only with day length and no effect of climatic factors on the number of nests in the colony was *** of nests varied significantly between successive years of *** the case of the Rook(predictable breeders),the photoperiod seems to be the most important factor influencing breeding behavior,especially nest *** the last three decades,increasing temperature and time of spring phases have advanced and have influenced changes in the phenology of the availability of food ***,a rapid decline in the numbers of breeding pairs of the Rook in Poland has been observed,especially in the last three *** attachment to the length of the day,which is the main factor responsible for the development of the breeding colony,makes the Rook appear to be a species with low plasticity in the face of rapid climate ***,we suggest that may indirectly influence the decrease in the breeding population of the Rook.

关键词： Breeding colony Climate change Corvus frugilegus Nest building Photoperiodism Predictable breeding