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Lignocellulolytic activities of a novel strain of <i>Trichoderma harzianum</i>

Advances in Bioscience and Biotechnology 2013年第2期4卷 214-221页

作者： M. Rubeena kannan neethu S. Sajith S. Sreedevi Prakasan Priji K. N. Unni M. K. Sarath Josh V. N. Jisha S. Pradeep Sailas Benjamin Enzyme Technology Laboratory Biotechnology Division Department of Botany University of Calicut Malappuram India

This study describes a novel dark-green spore producing strain of Trichoderma harzianum exhibiting higher activities of cellulase, hemicellulase and ligninase on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was supplemented with synthetic [carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose] and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO3 or NH4NO3) sources. Temperature and pH optima were 28?C and 4, respectively for the growth of the fungus in CMC- BSM with 146 U/ml cellulase activity. Flours of potato and banana supported comparable yields of cellulase to that of CMC (147 U/ml and 168 U/ml, respectively), while sodium nitrate was the preferred nitrogen source (150 U/ml). The water soluble yellowish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 414 nm. To comprise, this fungus shows the complete lignocellulolytic potential which offers great industrial significance, especially for the ethanol production from the lignocellulosic waste coupled with the production of a new pigment.

关键词： Trichoderma harzianum Morphology Submerged Fermentation Yellowish-Green Pigment

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A novel strain of <i>Trichoderma viride</i>shows complete lignocellulolytic activities

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1160-1166页

作者： kannan neethu M. Rubeena S. Sajith S. Sreedevi Prakasan Priji K. N. Unni M. K. Sarath Josh V. N. Jisha S. Pradeep Sailas Benjamin Enzyme Technology Laboratory Biotechnology Division Department of Botany University of Calicut Malappuram India

In this study we describe a novel dark-green strain of Trichoderma viride exhibiting complete ensemble of cellulase, hemicellulase and ligninase activities on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was fortified with synthetic (carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose) and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO3 or NH4NO3) sources. Temperature and pH optima were 28°C and 4, respectively for the growth of the fungus in CMC-BSM with 137 U/mL cellulase activity, which was enhanced to 173 U/mL at 1.25% CMC concentration. Flours of potato and banana peel supported comparable yields of cellulase to that of CMC, while sodium nitrate was the preferred nitrogen source. The water soluble bluish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 292 nm. To sum up, the complete lignocellulolytic potential of this fungus offers great industrial significance, coupled with the production of a new pigment.

关键词： Trichoderma viride Morphology Liquid Fermentation Bluish-Green Pigment

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