A novel strain of <i>Trichoderma viride</i>shows complete lignocellulolytic activities
A novel strain of <i>Trichoderma viride</i>shows complete lignocellulolytic activities作者机构：Enzyme Technology Laboratory Biotechnology Division Department of Botany University of Calicut Malappuram India
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2012年第3卷第8期
页 面：1160-1166页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Trichoderma viride Morphology Liquid Fermentation Bluish-Green Pigment
摘 要：In this study we describe a novel dark-green strain of Trichoderma viride exhibiting complete ensemble of cellulase, hemicellulase and ligninase activities on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was fortified with synthetic (carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose) and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO3 or NH4NO3) sources. Temperature and pH optima were 28°C and 4, respectively for the growth of the fungus in CMC-BSM with 137 U/mL cellulase activity, which was enhanced to 173 U/mL at 1.25% CMC concentration. Flours of potato and banana peel supported comparable yields of cellulase to that of CMC, while sodium nitrate was the preferred nitrogen source. The water soluble bluish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 292 nm. To sum up, the complete lignocellulolytic potential of this fungus offers great industrial significance, coupled with the production of a new pigment.
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