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Neurotoxicity and Biomarkers of Lead Exposure: a Review

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Chinese Medical Sciences Journal 2013年第3期28卷 178-188页

作者： kang-sheng liu Jia-hu Hao Yu Zeng Fan-chun Dai Ping-qing Gu State Key Laboratory of Reproductive Medicine Department of Clinical Laboratory Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Department of Maternity and Child Health Care School of Public HealthAnhui Medical University Child Health Care Department Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Obstetrical Department Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Department of Epidemiological and Health Statistics School of Public Health of Nanjing Medical University

Appropriate selection and measurement of lead biomarkers of exposure are critically important for health care management purposes,public health decision making,and primary prevention *** is one of the neurotoxicants that seems to be involved in the etiology of *** are generally classified into three groups:biomarkers of exposure,effect,and *** main body compartments that store lead are the blood,soft tissues,and bone;the half-life of lead in these tissues is measured in weeks for blood,months for soft tissues,and years for *** the brain,lead-induced damage in the prefrontal cerebral cortex,hippocampus,and cerebellum can lead to a variety of neurological disorders,such as brain damage,mental retardation,behavioral problems,nerve damage,and possibly Alzheimer’s disease,Parkinson’s disease,and *** paper presents an overview of biomarkers of lead exposure and discusses the neurotoxic effects of lead with regard to children and adults.

关键词： lead poisoning biological monitoring neurotoxicity neurodevelopment

Blood Lead Levels During Pregnancy and Its Influencing Factors in Nanjing,China

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Chinese Medical Sciences Journal 2013年第2期28卷 95-101页

作者： kang-sheng liu Jia-hu Hao Juan Shi Chun-fang Dai Xi-rong Guo State Key Laboratory of Reproductive Medicine Department of Clinical LaboratoryNanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Department of Maternity Child Health Care School of Public HealthAnhui Medical University Child Health Care Department Nanjing Maternity and Child Health Care Hospital Department of Obstetrics Nanjing Maternity and Child Health Care Hospital Director's Office Nanjing Maternity and Child Health Care Hospital

Objective To investigate the blood lead levels （BLLs） in the duration of pregnancy and 6-12 weeks after delivery, and analyze the influencing factors of BLLs in healthy pregnant women. Methods Pregnant women were recruited from September 2009 to February 2010 at the prenatal clinic in Nanjing Maternity and Child Health Care Hospital. Altogether 174 healthy pregnant women without pregnant or obstetric complications or abnormal pregnancy outcomes were enrolled as the gravida group, and 120 healthy non-pregnant women as the control group. BLLs during pregnancy were determined by flame atomic absorption spectroscopy. Results BLLs in all the three pregnancy trimesters and postpartum were 59.8±24.3, 55.4±20.1, 55.9±19.7, and 67.6±17.4 μ/L, respectively, and the mean BLL in control group was 67.5±21.3 μg/L. BLLs during all the three trimesters were lower in the gravida group than in the control group （P=0.043, 0.021, and 0.028）. Furthermore, occupations, nutrients supplementation, and time of house/apartment painted were associated with BLLs in pregnant women. Lead-related occupations, cosmetics use, and living in a house painted less than 1 year before are risk factors of high BLLs among pregnant women, while calcium, iron, zinc, and milk supplements are protective factors. Conclusion Supplementing calcium, iron, zinc, and milk, or avoiding contact with risk factors may help people, especially pregnant women, to reduce lead exposure.

关键词： blood lead levels pregnancy lead exposure

Evaluation of circulating tumor DNA as a biomarker for gynecological tumors

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Chinese Medical Journal 2020年第21期133卷 2613-2615页

作者： kang-sheng liu Hua Tong Tai-Ping Li Ya-Jun Chen Department of Clinical Laboratory Women's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu 210029China Department of Obstetrics and Gynecology Women's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu 210029China Department of Neuro-Psychiatric Institute The affiliated Brain HospitalNanjing Medical UniversityNanjingJiangsu 210029China

Circulating tumor DNA(ctDNA)is a DNA fragment released into the peripheral blood by apoptotic or necrotic tumor cells,which carry the basic genetic information after gene *** detection and analysis of ctDNA can provide genomic information on the size and development of tumor;therefore,it is considered as an emerging and promising tumor biomarker for cancer progression,reoccurrence,and routine monitoring after *** the isolation method is relatively simple due to the stability of ctDNA,the abundance of ctDNA is extremely low,and result from the high content of background cell free DNA(cfDNA),the large difference between individuals,and the need of predicted detection sites,it is necessary to analyze ctDNA in a comprehensive *** present,the main methods that meet the needs of ctDNA detection with sensitivity and specificity are amplification refractory mutation system PCR(ARMS-PCR),digital-PCR(dPCR)and high-throughput *** them,dPCR has advantages in practicality,which can realize absolute quantification of single-molecule DNA,detect and trace DNA molecules and conduct quantitative analysis,providing a reliable basis for clinical monitoring of tumor recurrence and minimal residual *** study reviews the detection and potential value of ctDNA in gynecologic tumors.

关键词： routine specificity absolute

Breast Milk Lead and Cadmium Levels in Suburban Areas of Nanjing,China

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Chinese Medical Sciences Journal 2013年第1期28卷 7-15页

作者： kang-sheng liu Jia-hu Hao Yu-qing Xu Xiao-qi Gu Juan Shi Chun-fang Dai Fei Xu Rong Shen State Key Laboratory of Reproductive Medicine Department of Clinical LaboratoryNanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Department of Maternity and Child Health Care School of Public HealthAnhui Medical University Director's Office Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Child Health Care Department Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Obstetrical Department Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Department of Clinical Laboratory Nanjing Children's Hospital

Objective To evaluate levels of lead(Pb) and cadmium(Cd) in the breast milk in the second postpartum month,to investigate the relationship between Pb/Cd levels in breast milk and some sociodemographic parameters,and to explore whether these levels affect the infants' physical status or the mothers' psychological status(postpartum depression).Methods A cross-sectional study was conducted between November 2009 and December *** 170 healthy mothers were enrolled from Nanjing Maternity and Child Health Care *** inclusion criteria were:voluntary to participate in this study,healthy,with no chronic disease,breastfeeding in the second postpartum month,living in a suburban but not non-industrial area of Nanjing,and not occupationally exposed to toxic *** the mothers completed a questionnaire and were evaluated based on the Edinburgh Postpartum Depression Scale(EPDS) to identify the risk of postpartum *** and Cd levels in breast milk were determined by inductively coupled plasma mass *** infants of these mothers were examined for their z scores of weight for age,length for age,head circumference for age,and body mass index for *** The median breast milk levels of Pb and Cd were 40.6 μg/L and 0.67 μg/L,*** 164(96.5%) of the 170 samples,Pb levels were higher than the limit reported by the World Health Organization(> 5 μg/L).Breast milk Cd level was > 1 μg/L in 54(31.8%) *** mothers with a history of anemia had a higher breast milk Pb level than those without a history of anemia(41.1 μg/L vs.37.9 μg/L,P = 0.050).The median breast milk Cd level in those who were active and passive smokers during pregnancy was significantly higher than that in non-smokers(0.88 μg/L vs.0.00 μg/L,P = 0.025).The breast milk Cd level in the mothers not taking iron and vitamin supplements for 2 months postpartum was higher than in those taking the supplements(iron supplement:0.74 μg/L vs.0.00 μg/L,P = 0.025;vitamin supplemen

关键词： lead cadmium suburban areas breast milk Edinburgh Postpartum Depres-sion Scale

Advances of Long Noncoding RNAs-mediated Regulation in Reproduction

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Chinese Medical Journal 2018年第2期131卷 226-234页

作者： kang-sheng liu Tai-Ping Li Hua Ton Xiao-Dong Mao Ya-Jun Chen Department of Clinical Laboratory State Key Laboratory of Reproductive Medicine Nanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210029 China Department of Pharmacy The Affiliated Friendship Plastic Surgery Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Department of Obstetrics and Gynecology State Key Laboratory of Reproductive Medicine Nanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medicar University Nanjing Jiangsu 210029 China Department of Endocrinology Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine Nanjing Jiangsu 210028 China

Objective： Advances in genomics and molecular biology have led to the discovery of a large group of uncharacterized long noncoding RNAs （lncRNAs）. Emerging evidence indicated that many lncRNAs function in multiple biological processes and its dysregulation otten causes diseases. Recent studies suggested that almost all regulatory lncRNAs interact with biological macromolecules such as DNA, RNA, and protein. LncRNAs regulate gene expression mainly on three levels, including epigenetic modification, transcription, and posttranscription, through DNA methylation, histone modification, and chromatin remodeling. LncRNAs can also affect the development of diseases and tllerefore be used to diagnose and treat diseases. With new sequencing and microarray techniques, hundreds oflncRNAs involved in reproductive disorders have been identified, but their functions in these disorders are undefined. Data Sources： This review was based on articles published in PubMed databases up to July 10, 2017, with the tbllowing keywords：＂long noncoding RNAs＂, ＂＇LncRNA＂, ＂placentation＂, and ＂＇reproductive diseases＂. Study Selection： Original articles and reviews on the topics were selected. Results： LncRNAs widely participate in various physiological and pathological processes as a new class of important regulatory/＇,actors. In spermatogenesis, spermatocytes divide and differentiate into mature spermatozoa. The whole process is elaborately regulated by the expression of phase-specific genes that involve many strains of lncRNAs. Literature showed that IncRNA in reproductive cumulus cells may contribute to the regulation of oocyte maturation, fertilization, and embryo development. Conclusions： LncRNA has been found to play a role in the development of reproduction. Meanwhile, we reviewed the studies on how lncRNAs participate in reproductive disorders, which provides a basis fbr the study of [ncRNA in reproduction regulation.

关键词： Cumulus Cell: Long Noncoding RNAs: Placentation: Reproductive Diseases

Pharmacokinetics of metadoxine for injection after repeated doses in healthy volunteers

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Chinese Medical Journal 2007年第2期120卷 166-168页

作者： Lü Yuan kang Zi-sheng liu Yan LI Tian-yun XIAO Yong-hong Institute of Clinical Pharmacology First Hospital Peking University Beijing 100083 China

Alcohol-induced liver disease is one of the main .epidemic problems nowadays. Metadoxine is apyridoxine-pyrrolidone carboxylate with significant scavenging property. Metadoxine is able to accelerate the elimination of alcohol from the blood and tissues, help restore the functional structure of the liver and relieve neuro-psychological disorders associated with alcohol *** purpose of the study was to assay the pharmacokinetics of domestic metadoxine after repeated doses.

关键词： metadoxine repeated doses pharmacokinetics healthy volunteer

The Influence of Sperm DNA Damage and Semen Homocysteine on Male Infertility

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Reproductive and Developmental Medicine 2017年第4期1卷 228-232页

作者： kang-sheng liu Feng Pan Ya-Jun Chen Xiao-Dong Mao Department of Clinical Laboratory Nanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu 210029China Department of Andrology State Key Laboratory of Reproductive MedicineNanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical UniversityNanjingJiangsu 210029China Department of Endocrinology Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese MedicineNanjingJiangsu 210028China

Background:To explore the relationship of sperm DNA fragmentation index(DFI),serum and seminal plasma homocysteine(Hcy),and semen parameters in patients with severe spermatogenetic ***:A total of 77 infertile males treated in our hospital for severe spermatogenetic dysfunction from January 2016 to November 2017 were *** involved patients were divided into two groups:oligozoospermia(SOM group,35 cases)and asthenozoospermia(OAT group,42 cases).The control group(NM group)contained 31 healthy males without reproductive *** the participants involved were tested in the items below:spermatozoa parameters,spermatozoa DFI,serum Hcy level and seminal plasma Hcy level,concentration of seminal plasma malondialdehyde(MDA),and total antioxidant capacity(TAC).Results:Between the SOM group and NM group,there were significantly difference in sperm concentration,motility and vitality,concentration of MDA,and *** spermatozoa DFI and Hcy levels in SOM group were significantly higher than those of the NM *** DFI was positively correlated with serum Hcy level(r=0.083,P0.05).Conclusions:The elevated Hcy level and spermatozoa DFI may be important factors of the severe spermatogenetic dysfunction,which can be used as semen index to evaluate sperm quality and male fertility.

关键词： DNA Fragmentation Index Homocysteine Male Infertility Routine Spermatozoa Parameters

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Reproductive and Developmental Medicine 2017年第1期1卷 18-22页

作者： kang-sheng liu Xiao-Dong Mao Feng Pan Ling-Juan Gao Xiu-Feng Ling Department of Clinical Laboratory State Key Laboratory of Reproductive MedicineNanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical UniversityNanjingJiangsu 210029China Department of Endocrinology Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjingJiangsu 210029China Department of Andrology State Key Laboratory of Reproductive MedicineNanjing Obstetrics and Gynecology HospitalAffiliated to Nanjing Medical UniversityNanjingJiangsu 210029China Department of Reproduction State Key Laboratory of Reproductive MedicineNanjing Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical UniversityNanjingJiangsu 210029China

Long noncoding RNAs(lncRNAs)are transcribed by RNA molecules,which are longer than 200 nucleotides that lack an open reading frame of significant length and possess no obvious protein-coding *** have shown that lncRNAs participate in many physiological processes such as gene imprinting and X chromosome *** regulate gene expression mainly through DNA methylation,histone modification,and chromatin *** can also affect the development of diseases,and they can be useful to diagnose and treat *** the development of new sequencing and microarray techniques,hundreds of lncRNAs involved in spermatogenesis have been identified,but their functions in the testis are ***,we will discuss the biology and regulation of lncRNAs,as well as the bioinformatics tools and searchable databases used to study them in the *** hope that this information will provide new insights in treating male reproductive diseases.

关键词： Epigenetics Long Noncoding RNA Long Noncoding RNA Database Spermatogenesis

植物生长调节剂、氮肥与密度配伍对辣椒产量及品质的影响

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江西农业大学学报 2009年第4期31卷 644-649页

作者：曾长立康六生江汉大学生命科学院湖北武汉430056 湖南省新化县农业局湖南新化417600

应用五因素五水平二次正交通用旋转组合设计,分析3种植物生长调节剂缩节胺(PIX)、萘乙酸(NAA)、防落素(PCPA)与氮肥(N)和密度(D)配伍对辣椒产量和品质的影响。结果表明:缩节胺、萘乙酸、防落素、氮肥与密度对辣椒产量均有明显的影响,其... 详细信息

应用五因素五水平二次正交通用旋转组合设计,分析3种植物生长调节剂缩节胺(PIX)、萘乙酸(NAA)、防落素(PCPA)与氮肥(N)和密度(D)配伍对辣椒产量和品质的影响。结果表明:缩节胺、萘乙酸、防落素、氮肥与密度对辣椒产量均有明显的影响,其中以氮肥与密度的影响最大;氮素、缩节胺、萘乙酸和防落素与辣椒素含量之间关系密切;氮肥、萘乙酸和防落素对果实中Vc含量有明显的作用;氮素、缩节胺、防落素和萘乙酸对硝酸盐含量有显著影响。同时,各影响因子之间还存在显著的正交互作用或负交互作用。

关键词：辣椒产量品质植物生长调节剂氮肥密度