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Efficient derivation of embryonic stem cells from NOD-scid 112rg mice

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Protein & Cell 2015年第12期6卷 916-918页

作者： kang liu (1) (2) Riguo Fang (1) (2) Haibo Li (1) Weifeng Yang (4) Zhenchuan Miao (4) Jinhua Wen (1) Hongkui Deng (1) (2) (3) 1.Peking University Stem Cell Research Center Department of Cell Biology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China 2.The MOE Key Laboratory of Cell Proliferation and Differentiation College of Life Sciences Peking-Tsinghua Center for Life Sciences Peking University Beijing 100871 China 4.VITALSTAR Bitechnology Co. Ltd Beijing China 3.Shenzhen Stem Cell Engineering Laboratory Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Shenzhen 518055 China

For decades, the stem cell field has pursued the development of humanized mice to further enhance our understanding of human hematopoiesis, innate immunity, infectious diseases, cancer biology and regenerative medicine. One significant breakthrough in generating humanized mice was the development of NOD-scid 112rg mice, which are double homozygous for the severe combined immun- odeficiency （SCID） mutation and interleukin-2Rg （11-2Rg） allelic mutation （gamma c null）（Ito et al., 2002）.

关键词： derivation stem mice

Regional gray matter abnormality in hepatic myelopathy patients after transjugular intrahepatic portosystemic shunt: a voxel-based morphometry study

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Neural Regeneration Research 2019年第5期14卷 850-857页

作者： kang liu Gang Chen Shu-Yao Ren Yuan-Qiang Zhu Tian-Lei Yu Ping Tian Chen Li Yi-Bin Xi Zheng-Yu Wang Jian-Jun Ye Guo-Hong Han Hong Yin Department of Radiology Xijing Hospital Air Force Military Medical University (Fourth Military Medical University) Department of Radiology Lanzhou General Hospital Lanzhou Military Command Xijing Hospital of Digestive Diseases Air Force Military Medical University (Fourth Military Medical University) Life Sciences Research Center School of Life Sciences and Technology Xidian University

Hepatic myelopathy is a complication seen in patients with chronic liver failure with physiologic or iatrogenic portosystemic shunting. The main symptom is progressive lower limb dyskinesia. The role of the brain motor control center in hepatic myelopathy is unknown. This study aimed to investigate the gray matter changes in patients with hepatic myelopathy secondary to transjugular intrahepatic portosystemic shunt and to examine their clinical relevance. This was a cross-sectional study. Twenty-three liver failure patients with hepatic myelopathy(hepatic myelopathy group), 23 liver failure patients without hepatic myelopathy(non-hepatic myelopathy group) after transjugular intrahepatic portosystemic shunt, and 23 demographically matched healthy volunteers were enrolled from March 2014 to November 2016 at Xijing Hospital, Air Force Military Medical University(Fourth Military Medical University), China. High-resolution magnetization-prepared rapid gradient-echo brain imaging was acquired. Group differences in regional gray matter were assessed using voxel-based morphometry analysis. The relationship between aberrant gray matter and motor characteristics was investigated. Results demonstrated that compared with the non-hepatic myelopathy group, gray matter volume abnormalities were asymmetric, with decreased volume in the left insula(P = 0.003), left thalamus(P = 0.029), left superior frontal gyrus(P = 0.006), and right middle cingulate cortex(P = 0.021), and increased volume in the right caudate nucleus(P = 0.017), corrected with open-source software. The volume of the right caudate nucleus in the hepatic myelopathy group negatively correlated with the lower limb clinical rating of the Fugl-Meyer Assessment(r = –0.53, P = 0.01). Compared with healthy controls, patients with and without hepatic myelopathy exhibited overall increased gray matter volume in both thalami, and decreased gray matter volume in both putamen, as well as in the globus pallidus, cerebellum, and

关键词： portosystemic shunt hepatic myelopathy hepatic encephalopathy magnetic resonance imaging gray matter lower limb Fugl-Meyer Assessment basal ganglia caudate nucleus voxel-based morphometry

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基于数据挖掘的电子商务交易风险评估

现代电子技术 2017年第5期40卷 74-77,81页

作者：刘抗商丘工学院管理学院河南商丘476000

交易风险评估是保证电子商务系统正常运行的基础,当前电子商务交易风险评估存在准确性差、结果不可靠等难题,为了提高电子商务交易风险评估的正确率,提出基于数据挖掘的电子商务交易风险评估模型。对当前电子商务交易风险评估研究情况... 详细信息

交易风险评估是保证电子商务系统正常运行的基础,当前电子商务交易风险评估存在准确性差、结果不可靠等难题,为了提高电子商务交易风险评估的正确率,提出基于数据挖掘的电子商务交易风险评估模型。对当前电子商务交易风险评估研究情况进行分析,并构建电子商务交易风险的评估指标体系;根据电子商务交易风险评估指标收集数据,并采用数据挖掘技术——回声状态网络建立电子商务交易风险评估模型;最后对电子商务交易风险评估性能进行分析,回声状态网络可以对电子商务交易过程中存在的风险进行有效挖掘,发现风险变化态势,评估结果可以为电子商务交易人员提供有用的信息。

关键词：电子商务交易风险回声状态网络评估指标

关于城乡统筹中如何促进新老市民和谐相处的思考

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探索 2008年第5期 116-119页

作者：刘康中共重庆市委党校重庆400041

如何促进新老市民的和谐相处,使新市民尽快融入城市生活,是城乡统筹中必须认真研究的一个重要课题。本文认为,确立"城里人,乡下人,都是一家人"的重要理念,应当成为处理新老市民关系、促进城市社会和谐的思想基础。促进新老市民的和谐相... 详细信息

如何促进新老市民的和谐相处,使新市民尽快融入城市生活,是城乡统筹中必须认真研究的一个重要课题。本文认为,确立"城里人,乡下人,都是一家人"的重要理念,应当成为处理新老市民关系、促进城市社会和谐的思想基础。促进新老市民的和谐相处,需要老市民、新市民和政府三方共同努力:老市民要尊重帮助新市民,新市民要融入城市做主人,政府则应统筹安排促和谐。

关键词：城乡统筹新市民老市民和谐相处

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镜中记

十月 2023年第5期 236-237页

作者：刘康不详

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现场总线技术在楼宇自动化中的应用

微计算机信息 2007年第28期23卷 170-172页

作者：刘康华南理工大学

现场总线是企业底层数字通讯网络,是连接微机化仪表的开放系统。从一定意义上说,智能仪表就相当于一台台微机,它们以现场总线为纽带,连成网络系统,完成数字通讯任务,可以说现场总线系统就是控制领域的计算机局域网络。本文详细阐述了Lon... 详细信息

现场总线是企业底层数字通讯网络,是连接微机化仪表的开放系统。从一定意义上说,智能仪表就相当于一台台微机,它们以现场总线为纽带,连成网络系统,完成数字通讯任务,可以说现场总线系统就是控制领域的计算机局域网络。本文详细阐述了LonWorks总线和其技术特点及原理,我将LonWorks技术应用在楼宇自动化系统中。

关键词：现场总线底层网络开放性互用性 LonWorks 宇自动化

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一种改进的无模型自适应控制优化方法

北京化工大学学报（自然科学版） 2023年第6期50卷 66-73页

作者：刘康陈娟北京化工大学信息科学与技术学院北京100029

针对一类复杂非线性系统提出了一种改进的无模型自适应控制(improved model⁃free adaptive control,IM⁃FAC)方法,该方法通过对传统无模型自适应控制(model⁃free adaptive control,MFAC)方法控制器输出准则函数引入系统输出变化量来改善... 详细信息

针对一类复杂非线性系统提出了一种改进的无模型自适应控制(improved model⁃free adaptive control,IM⁃FAC)方法,该方法通过对传统无模型自适应控制(model⁃free adaptive control,MFAC)方法控制器输出准则函数引入系统输出变化量来改善系统的动态性能。分析了IMFAC方法的稳定性和收敛性,并针对IMFAC方法中5个参数整定困难的问题,提出了基于灰狼优化(grey wolf optimization,GWO)算法的控制器参数优化方法(G-IMFAC)。对于GWO适应度函数中3个优化指标权重的最优分配问题,通过计算各个优化指标的信息熵来确定其权重。最后通过仿真模拟对比了G-IMFAC方法与传统MFAC方法及IMFAC方法的性能,结果表明G-IMFAC方法具有更小的超调量和更短的调节时间,进一步提高了系统的控制性能。

关键词：非线性系统无模型自适应控制参数优化熵权法

FS-Net:面向时序知识图谱推理的频次统计网络

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软件学报 2023年第10期34卷 4518-4532页

作者：刘康正赵峰金海大数据技术与系统国家地方联合工程研究中心湖北武汉430074 服务计算技术与系统教育部重点实验室湖北武汉430074 集群与网格计算湖北省重点实验室湖北武汉430074 华中科技大学计算机科学与技术学院湖北武汉430074

时序知识图谱推理吸引了研究人员的极大关注.现有的时序知识图谱推理技术通过建模历史信息取得了巨大的进步.但是,时变性问题和不可见实体(关系)问题仍然是阻碍时序知识图谱推理模型性能进一步提升的两大挑战;而且由于需要对历史子图序... 详细信息

时序知识图谱推理吸引了研究人员的极大关注.现有的时序知识图谱推理技术通过建模历史信息取得了巨大的进步.但是,时变性问题和不可见实体(关系)问题仍然是阻碍时序知识图谱推理模型性能进一步提升的两大挑战;而且由于需要对历史子图序列的结构信息和时间依赖信息进行建模,传统的基于嵌入的方法往往在训练和预测过程中具有较高的时间消耗,这极大地限制了推理模型在现实场景中的应用.针对以上困境,提出了一个用于时序知识图谱推理的频次统计网络,FS-Net.一方面,FS-Net不断基于最新的短期历史的事实频次统计,动态地为变化的时间戳上的预测生成时变的得分;另一方面,FS-Net基于当前时间戳上的事实频次统计,为预测补充历史不可见实体(关系);特别地,FS-Net不需要进行训练,而且具有极高的时间效率.在两个时序知识图谱基准数据集上的大量实验,表明了FS-Net相较于基准模型的巨大提升.

关键词：时序知识图谱事实频次统计时变性不可见信息

Schizandra arisanensis extract attenuates cytokine-mediated cytotoxicity in insulin-secreting cells

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World Journal of Gastroenterology 2012年第46期18卷 6809-6818页

作者： Yi-Shin Hsu Yao-Haur Kuo Hui-Ling Cheng Peter R Flatt Hui-kang liu Division of Herbal Drugs and Natural Products National Research Institute of Chinese Medicine Graduate Institute of Integrated Medicine China Medical University School of Biomedical Sciences University of Ulster

AIM: To explore the bioactivity of an ethanolic extract of Schizandra arisanensis (SA-Et) and isolated constituents against interleukin-1 β and interferon-γ-mediated β cell death and abolition of insulin secretion. METHODS: By employing BRIN-BD11 cells, the effects of SA-Et administration on cytokine-mediated cell death and abolition of insulin secretion were evaluated by a viability assay, cell cycle analysis, and insulin assay. The associated gene and protein expressions were also measured. In addition, the bioactivities of several peak compounds collected from the SA-Et were tested against cytokine-mediated β cell ***: Our Results revealed that SA-Et dose-dependently ameliorated cytokine-mediated β cell death and apoptosis. Instead of suppressing inducible nitric oxide synthase/nitric oxide cascade or p38MAPK activity, suppression of stress-activated protein kinase/c-Jun NH2-terminal kinase activity appeared to be the target for SA-Et against the cytokine mix. In addition, SA-Et provided some insulinotropic effects which re-activated the abolished insulin exocytosis in cytokine-treated BRIN-BD11 cells. Finally, schiarisanrin A and B isolated from the SA-Et showed a dose-dependent protective effect against cytokine-mediated β cell death. CONCLUSION: This is the first report on SA-Et ameliorating cytokine-mediated β cell death and dysfunction via anti-apoptotic and insulinotropic actions.

关键词： Schizandra arisanensis C19 homolignans Type 1 diabetes Insulin-secreting cells Interleukin-1β Interferon-γ