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Cellular & Molecular Immunology 2017年第12期14卷 1023-1025页

作者： xilong kang Zhiming Pan Xinan Jiao Jiangsu Key Laboratory of Zoonosis YangzhouUniversityYangzhouJiangsu 225009China Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhouJiangsu 225009China Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agrifood Safety and Quality MOAYangzhou UniversityYangzhouJiangsu 225009China Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education YangzhouJiangsu 225009China

Flagellin,the main component of bacterial flagella,is a well-known pathogen-associated molecular pattern(PAMP)that can be recognized by the cell surface-localized Toll-like receptor 5(TLR5)and the cytosolic NOD-like receptor protein 4(NLRC4)inflammasome receptor NAIP5/6.1 Hosts can detect flagellated bacteria using the dual sensors,TLR5 and NAIP5/6,to recognize flagellin,and these sensors then initiate innate immune responses to protect the host from bacterial *** by extracellular TLR5 can trigger the MyD88-dependent signaling pathway and activate nuclear factorκB,leading to the upregulation of several proinflammatory cytokines.

关键词： effect TLR5 initiate

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猪γ干扰素单克隆抗体的制备与特性研究

黑龙江畜牧兽医 2024年第5期 80-85页

作者：沈环环韩顺子孟闯潘志明焦新安康喜龙扬州大学江苏省人兽共患病学重点实验室江苏扬州225009 扬州大学农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室江苏扬州225009 扬州大学江苏省动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

为了制备高特异性的抗猪γ干扰素(IFN-γ)抗体,试验采用原核表达的携带His标签的重组猪IFN-γ(PoIFN-γ)蛋白免疫小鼠,利用杂交瘤技术制备杂交瘤细胞;并以携带GST标签的重组蛋白PoIFN-γ(GST-PoIFN-γ)为包被抗原,通过间接ELISA方法筛... 详细信息

为了制备高特异性的抗猪γ干扰素(IFN-γ)抗体,试验采用原核表达的携带His标签的重组猪IFN-γ(PoIFN-γ)蛋白免疫小鼠,利用杂交瘤技术制备杂交瘤细胞;并以携带GST标签的重组蛋白PoIFN-γ(GST-PoIFN-γ)为包被抗原,通过间接ELISA方法筛选稳定分泌抗体的单克隆细胞株;而后通过间接ELISA方法、单克隆抗体亚型鉴定试剂盒、SDS-PAGE分析、Western-blot鉴定和间接免疫荧光方法对获得的单克隆抗体的腹水效价、亚型、反应特异性等生物学特性进行鉴定。结果表明:经筛选后共获得7株能稳定分泌PoIFN-γ的单克隆抗体细胞株;7株单克隆抗体的腹水效价在1∶2048000~1∶16384000之间,均为IgG1亚型,均能与原核表达及真核表达的PoIFN-γ蛋白发生特异性反应且反应性良好。说明试验成功制备了可特异性识别PoIFN-γ的单克隆抗体。

关键词：猪γ干扰素原核表达单克隆抗体效价特异性

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鸡白细胞介素-2原核表达及其活性鉴定

中国家禽 2024年第5期46卷 56-61页

作者：韩顺子黄霞孟闯耿士忠康喜龙潘志明焦新安扬州大学江苏省人兽共患病学重点实验室江苏扬州225009 扬州大学农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

试验旨在获得具有生物活性的鸡白细胞介素-2(ChIL-2)重组蛋白。试验以克隆获得的ChIL-2基因为模板,分别构建原核重组表达菌BL21(pCold-ChIL-2)和BL21(pGEX-6P-1-ChIL-2),进行诱导表达和纯化;通过BrdU ELISA法检测重组蛋白诱导鸡脾脏淋... 详细信息

试验旨在获得具有生物活性的鸡白细胞介素-2(ChIL-2)重组蛋白。试验以克隆获得的ChIL-2基因为模板,分别构建原核重组表达菌BL21(pCold-ChIL-2)和BL21(pGEX-6P-1-ChIL-2),进行诱导表达和纯化;通过BrdU ELISA法检测重组蛋白诱导鸡脾脏淋巴细胞的增殖情况,评估其生物活性;以rHis-ChIL-2免疫小鼠制备多克隆抗体。结果显示:成功构建了原核重组表达菌BL21(pCold-ChIL-2)、BL21(pGEX-6P-1-ChIL-2);经表达纯化后分别获得纯度较高的rHis-ChIL-2和rGST-ChIL-2蛋白,大小分别为14 ku和39.4 ku;rHis-ChIL-2和rGST-ChIL-2蛋白分别能够促进脾脏淋巴细胞显著或极显著增殖(P<0.01或P<0.001),均具有良好的生物活性;以纯化的rHis-ChIL-2免疫小鼠制备的多克隆抗体效价为5.12×105,可识别rHis-ChIL-2和rGST-ChIL-2,与真核表达的ChIL-2蛋白反应。结果表明,原核表达的重组蛋白ChIL-2具有良好的生物活性,采用其制备的多克隆抗体可识别真核表达的ChIL-2蛋白,为后期ChIL-2的检测奠定基础。

关键词： ChIL-2 原核表达生物活性多克隆抗体

Amino acids 89-96 of Salmonella typhimunum Tlagell=n represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response

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Cellular & Molecular Immunology 2015年第5期12卷 625-632页

作者： Lei Zhang Zhiming Pan xilong kang Yun Yang Heekap kang Na Zhang James M Rosati Xinan Jiao Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou China Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China Ministry of Education Key Lab for Avian Preventive Medicine Yangzhou University Yangzhou China Division of Nephrology and Hypertension Department of Medicine Northwestern University Feinberg School of Medicine Chicago IL USA

Toll-like receptor 5 （TLR5） signaling in response to flagellin is dispensable for inducing humoral immunity, but alterations of aa 89-96, the TLR5 binding site, significantly reduced the adjuvanticity of flagellin. These observations indicate that the underlying mechanism remains incompletely understood. Here, we found that the native form of Salmonella typhimurium aa 89-96-mutant flagellin extracted from flagella retains some TLR5 recognition activity, indicating that aa 89-96 is the primary, but not the only site that imparts TLR5 activity. Additionally, this mutation impaired the production of IL-lp and IL-18. Using TLR5KO mice, we found that aa 89-96 is critical for the humoral adjuvant effect, but this effect was independent of TLR5 activation triggered by this region of flageUin. In summary, our findings suggest that aa 89-96 of flagellin is not only the crucial site responsible for TLR5 recognition, but is also important for humoral immune adjuvanticity through a TLR5-independent pathway.

关键词： adjuvanticity amino acids 89-96 Salmonella typhimurium flagellin TLR5 activity

鸡球虫重组蛋白rEnApiAP2对鸡免疫保护效果的观察

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畜牧兽医学报 2024年第4期55卷 1716-1727页

作者：王乐乐王礼跃蔡为民康喜龙冯茜茜张知之范雪莲朱玉刘丹丹许金俊潘志明陶建平扬州大学兽医学院扬州225009 江苏动物重要疫病与人兽共患病防控协同创新中心扬州225009 江苏省人兽共患病学重点实验室扬州225009 宿迁学院宿迁223800

将毒害艾美耳球虫重组蛋白rEnApiAP2单独免疫(单免)或分别与柔嫩艾美耳球虫重组蛋白rEtGAM56或rEtGAM59联合免疫(联免)雏鸡,同时设rEtGAM56单免、rEtGAM59单免、未免疫攻虫和空白组为对照,分别攻毒害艾美耳球虫或柔嫩艾美耳球虫,以成活... 详细信息

将毒害艾美耳球虫重组蛋白rEnApiAP2单独免疫(单免)或分别与柔嫩艾美耳球虫重组蛋白rEtGAM56或rEtGAM59联合免疫(联免)雏鸡,同时设rEtGAM56单免、rEtGAM59单免、未免疫攻虫和空白组为对照,分别攻毒害艾美耳球虫或柔嫩艾美耳球虫,以成活率、排出血便数、平均增重、相对增重率、病变记分、卵囊减少率、抗球虫指数(ACI)和血清抗体水平为指标,评价rEnApiAP2单免及与rEtGAM联免的免疫保护效果。结果显示:各试验组的成活率均为100%;与未免疫攻虫组比较,各免疫组鸡排血便堆数减少、平均增重增加。在rEnApiAP2单免组间,低剂量组的相对增重率(82.36%)、卵囊减少率(73.88%)和抗球虫指数(155.26)均为最高,平均病变记分最低(1.71)。攻毒害艾美耳球虫后,联免组的ACI值均大于相应的单免组,其中rEnApiAP2与rEtGAM56联免组的ACI值(169.83)最高。攻柔嫩艾美耳球虫后,rEnApiAP2单免组的ACI值(128.37)低于rEtGAM56、rEtGAM59单免组(130.12、151.88),rEnApiAP2与rEtGAM59联免组的ACI值(141.62)接近于rEtGAM59单免组,rEnApiAP2与rEtGAM56联免组的ACI值(115.46)小于rEtGAM56单免组。各免疫组血清抗体水平均显著高于空白对照组(P<0.05)。rEnApiAP2对毒害艾美耳球虫具有一定的免疫保护效果,与rEtGAM联免可提高对毒害艾美耳球虫的免疫保护力。本研究结果为鸡球虫病重组亚单位疫苗研制奠定了基础。

关键词：毒害艾美耳球虫柔嫩艾美耳球虫 rEnApiAP2 rEtGAM 保护效果

Field Load Test Based SHM System Safety Standard Determination for Rigid Frame Bridge

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Structural Durability & Health Monitoring 2024年第3期18卷 361-376页

作者： xilong Zheng Qiong Wang Di Guan School of Civil and Architectural Engineering Harbin UniversityHarbin150076China

The deteriorated continuous rigid frame bridge is strengthened by external prestressing. Static loading tests wereconducted before and after the bridge rehabilitation to verify the effectiveness of the rehabilitation process. Thestiffness of the repaired bridge is improved, and the maximum deflection of the load test is reduced from 37.9 to27.6 mm. A bridge health monitoring system is installed after the bridge is reinforced. To achieve an easy assessmentof the bridge’s safety status by directly using transferred data, a real-time safety warning system is createdbased on a five-level safety standard. The threshold for each safety level will be determined by theoretical calculationsand the outcomes of static loading tests. The highest risk threshold will be set at the ultimate limit statevalue. The remaining levels, namely middle risk, low risk, and very low risk, will be determined usingreduction coefficients of 0.95, 0.9, and 0.8, respectively.

关键词： Continuous rigid frame bridge rehabilitation long-term monitoring field load test safety standard determination

Study on the performance of lightweight roadway wall thermal insulation coating containing EP-GHB mixed ceramsite

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Building Simulation 2024年第5期17卷 785-798页

作者： Yongliang Zhang Shili Yin Hongwei Mu xilong Zhang Qinglei Tan Bing Shao Key Lab of Industrial Fluid Energy Conservation and Pollution Control Ministry of EducationQingdao University of TechnologyQingdaoShandong266520China College of Mechanical and Automotive Engineering Qingdao University of TechnologyQingdaoShandong266520China

As the mining depth increases,the problem of high-temperature thermal damage mainly caused by heat dissipation of surrounding rock is becoming more and more *** is very important to solve the environmental problem of mine heat damage to improve the efficiency of mineral resource exploitation and protect the physical and mental health of *** can apply thermal insulation coating on the walls of mine roadways as a means of implementing active heat *** this paper,expanded perlite(EP)and glazed hollow bead(GHB)are used as the main thermal insulation materials,ceramsite and sand as aggregate,plus glass fiber and sodium dodecyl sulfate to develop a new lightweight composite thermal insulation coating through orthogonal experiment *** to the plate heat flow meter method and mechanical test method,the thermal insulation and mechanical properties of EP-GHB mixed ceramsite coating were studied by making specimens with different parameter ratios,and according to the analysis of the experimental results,the optimal mix ratio of the coating was *** addition,Fluent numerical simulation software was used to establish the roadway model,and the thermal insulation effect of the coating in the roadway under different working conditions was *** results show that the thermal conductivity of the prepared composite thermal insulation coating material is only 8.5% of that of ordinary cement mortar,and the optimal thickness of adding thermal insulation coating is 0.2 m,which can reduce the outlet air temperature of the roadway with a length of 1000 m by 4.87 K at this *** thermal insulation coating developed in this study has the advantages of simple technology and strong practicability,and has certain popularization and application value in mine heat damage control.

关键词： high-temperature mine thermal insulation coating orthogonal experimental optimal fit ratio numerical simulation

Improved observation of colonized roots reveals the regulation of arbuscule development and senescence by drought stress in the arbuscular mycorrhizae of citrus

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Horticultural Plant Journal 2024年第2期10卷 425-436页

作者： xilong Yin Wei Zhang Zengwei Feng Guangda Feng Honghui Zhu Qing Yao College of Horticulture South China Agricultural UniversityKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China)Ministry of Agriculture and Rural AffairsGuangdong Province Key Laboratory of Microbial Signals and Disease ControlGuangzhouGuangdong 510642China Key Laboratory of Agricultural Microbiomics and Precision Application Ministry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationKey Laboratory of Agricultural MicrobiomicsState Key Laboratory of Applied Microbiology Southern ChinaGuangdong Microbial Culture Collection Center(GDMCC)Institute of MicrobiologyGuangdong Academy of SciencesGuangzhouGuangdong 510070China

Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to drought stress, remain unclear, which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method. Here, we improved the observation of colonized roots of citrus plants with the sectioning method, which enabled the clear observation of AM fungal structures. Furthermore, we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method. Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization (F%and M%) although it did not affect plant growth performance. Fluorescence probes (WGA 488 and/or Nile red) revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages. Meanwhile, drought stress accelerated arbuscule senescence, which was characterized by the increased accumulation of neutral lipids. Overall, the sectioning method developed in this study enables the in-depth investigation of arbuscule status, and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants. This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses.

关键词： Arbuscular mycorrhizae Citrus Drought stress Arbuscule development Arbuscule senescence Fruit tree species

Perspective on powder technology for all-solid-state batteries:How to pair sulfide electrolyte with high-voltage cathode

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Particuology 2024年第3期86卷 55-66页

作者： Jiangkui Hu Shijie Yang Yingying Pei xilong Wang Yulong Liao Shuai Li Aolong Yue Jia-Qi Huang Hong Yuan School of Materials Science&Engineering Beijing Institute of TechnologyBeijing100081China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing100081China

Sulfide solid electrolytes(SEs)have attracted ever-increasing attention due to their superior roomtemperature ionic conductivity(~10^(-2) S cm^(-1)).Additionally,the integration of sulfide SEs and highvoltage cathodes is promising to achieve higher energy ***,the incompatible interfaces between sulfide SEs and high-voltage cathodes have been one of the key factors limiting their ***,this review presents a critical summarization of the interfacial issues in all-solid-state lithium batteries based on sulfide SEs and high-voltage cathodes and proposes strategies to stabilize the electrolyte/cathode ***,the future research direction of electrolyte/cathode interfaces and application prospects of powder technology in sulfide-based ASSLBs were also discussed.

关键词： Sulfide solid electrolytes High-voltage cathodes Electrode/electrolyte interfaces All-solid-state lithium batteries

Pressure-triggered stacking dependence of interlayer coupling in bilayer WS_(2)

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 177-186页

作者： Zejuan Zhang Chenyin Jiao Shenghai Pei xilong Zhou Jiaze Qin Wanli Zhang Yu Zhou Zenghui Wang Juan Xia School of Integrated Sciences and Engineering(Exemplary School of Microelectronics) University of Electronic Science and Technology of ChinaChengdu 610054China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of ChinaChengdu 611731China School of Physics and Electronics Hunan Key Laboratory of Nanophotonics and DevicesCentral South UniversityChangsha 410083China

Tungsten disulfide(WS_(2))has been reported to show negligible stacking dependence under ambient conditions,impeding its further explorations on physical properties and potential ***,we realize efficient modulation of interlayer coupling in bilayer WS_(2)with 3R and 2H stackings by high pressure,and find that the pressure-triggered interlayer coupling and pressure-induced resonant-to-nonresonant transition exhibit prominent stacking dependence,which are experimentally observed for the first time in *** work may unleash the stacking degree of freedom in designing WS_(2)devices with tailored properties correlated to interlayer coupling.

关键词： tungsten disulfide pressure engineering stacking order interlayer coupling