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静电相互作用有助于蛋白质的热稳定性

静电相互作用有助于蛋白质的热稳定性

The structure of acidic ribosomal P protein and Its Interaction with ribosome-inactivating protein

中国晶体学会第四届全国会员代表大会暨学术会议

作者： kam-bo wong Department of Biochemistry The Chinese University of Hong Kong

＜正＞It has been long assumed that protein is stabilized mainly by the hydrophobic interaction among core residues,while the surface *** interactions contribute little to protein *** we use a ribosomal protein L30e from Thermococcus celer as a model to investigate the electrostatic contributions to protein *** of *** L30e determined by both NMR spectroscopy and X-ray crystallography reveals that the thermophilic homologue has more charge-charge ***-directed mutagenesis studies demonstrated that charge-charge interactions contribute to the thermostability of T.

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The structure of acidic ribosomal P protein and Its Interact...

Computational Approaches for Traditional Chinese Painting: From the “Six Principles of Painting” Perspective

21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology

作者： kam-bo wong Ka-Ming Lee Priscllia.Hui-Mei Too Amanda Nga-Sze Mak Pang-Chul Shaw Department of Biochemistry and Centre for Protein Science and Crystallography Faculty of ScienceThe Chinese University of Hong Kong

＜正＞Ribosome-inactivating proteins（RIPs） inhibit protein synthesis by enzymatically depurinating Adenine-4324 residue at the 28s *** systematic deletion analysis,our group has found that trichosanthin（TCS）,a ribosome-inactivating protein with multiple pharmacological properties,interacts with the C-terminal region of the ribosomal P2 protein,which is conserved among

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Journal of Computer Science & Technology 2024年第2期39卷 269-285页

作者：张玮张建伟黄锦畦王懿芳封颖超杰王璐玮陈为 State Key Laboratory of CAD&CG Zhejiang UniversityHangzhou 310058China Department of Computer Science and Engineering The Hong Kong University of Science and Technology Hong Kong 999077China Kellogg School of Management Northwestern UniversityEvanston 60208U.S.A Laboratory of Art and Archaeology Image Zhejiang UniversityHangzhou 310058China

Traditional Chinese painting(TCP)is an invaluable cultural heritage resource and a unique visual art *** recent years,there has been a growing emphasis on the digitalization of TCP for cultural preservation and *** resulting digital copies have enabled the advancement of computational methods for a structured and systematic understanding of *** explore this topic,we conduct an in-depth analysis of 94 pieces of *** examine the current use of computer technologies on TCP from three perspectives,based on numerous conversations with ***,in light of the“Six Principles of Painting”theory,we categorize the articles according to their research focus on artistic ***,we create a four-stage framework to illustrate the purposes of TCP ***,we summarize the popular computational techniques applied to *** work also provides insights into potential applications and prospects,with professional opinion.

关键词： traditional Chinese painting(TCP) digital humanities cultural heritage computer vision deep learning

Chemical looping reforming of the micromolecular component from biomass pyrolysis via Fe_(2)O_(3)@SBA-16

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International Journal of Coal Science & Technology 2024年第3期11卷 120-134页

作者： Yunchang Li bo Zhang Xiantan Yang bolun Yang Shengyong Zhang Zhiqiang Wu Shaanxi Key Laboratory of Energy Chemical Process Intensification School of Chemical Engineering and TechnologyXi’an Jiaotong UniversityXi’an710049ShaanxiPeople’s Republic of China

To solve the problems of low gasification efficiency and high tar content caused by solid–solid contact between biomass and oxygen carrier in traditional biomass chemical looping gasification *** decoupling strategy was adopted to decouple the biomass gasification process,and the composite oxygen carrier was prepared by embedding Fe_(2)O_(3) in molecular sieve SBA-16 for the chemical looping reforming process of pyrolysis micromolecular model compound methane,which was expected to realize the directional reforming of pyrolysis volatiles to prepare hydrogen-rich *** analysis of the reaction system was carried out based on the Gibbs free energy minimization method,and the reforming performance was evaluated by a fixed bed reactor,and the kinetic parameters were solved based on the gas–solid reaction *** analysis verified the feasibility of the reaction and provided theoretical guidance for experimental *** experimental results showed that the reaction performance of Fe_(2)O_(3)@SBA-16 was compared with that of pure Fe_(2)O_(3) and Fe_(2)O_(3)@SBA-15,and the syngas yield was increased by 55.3%and 20.7%respectively,and it had good cycle *** analysis showed that the kinetic model changed from three-dimensional diffusion to first-order reaction with the increase of *** activation energy was 192.79 kJ/mol by *** paper provides basic data for the directional preparation of hydrogen-rich syngas from biomass and the design of oxygen carriers for pyrolysis of all-component chemical looping reforming.

关键词： Biomass pyrolysis Methane Chemical looping reforming Oxygen carrier Kinetic analysis

Continuous-wave operation of 1550 nm low-threshold triple-lattice photonic-crystal surface-emitting lasers

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Light(Science & Applications) 2024年第3期13卷 446-453页

作者： Ziye Wang Xia Liu Pinyao Wang Huanyu Lu bo Meng Wei Zhang Lijie Wang Yanjing Wang Cunzhu Tong State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Central Research Institute Planning Dept 2012 LabsHuawei Technologies Company Ltd.Shenzhen 518129China

Benefitting from narrow beam divergence,photonic crystal surface-emitting lasers are expected to play an essential role in the ever-growing fields of optical communication and light detection and *** operating with 1.55μm wavelengths have attracted particular attention due to their minimum fiber loss and high eye-safe ***,high interband absorption significantly decreases their performance at this 1.55μm ***,stronger optical feedback is needed to reduce their threshold and thus improve the output *** this goal,photonic-crystal resonators with deep holes and high dielectric contrast are often ***,the relevant techniques for high-contrast photonic crystals inevitably complicate fabrication and reduce the final *** this paper,we demonstrate the first continuous-wave operation of 1.55μm photonic-crystal surface-emitting lasers by using a‘triple-lattice photonic-crystal resonator’,which superimposes three lattice point groups to increase the strength of in-plane optical *** this geometry,the in-plane 180°coupling can be enhanced threefold compared to the normal single-lattice *** theoretical and experimental investigations demonstrate the much lower threshold current density of this structure compared to‘single-lattice’and‘double-lattice’photonic-crystal resonators,verifying our design *** findings provide a new strategy for photonic crystal laser miniaturization,which is crucial for realizing their use in future high-speed applications.

关键词： resonator crystal lattice

Schematic interface of sound creation for computer animators

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第7期7卷 1141-1151页

作者： TONG kam-pang Maya wong kam-wah School of Creative Media City University of Hong Kong Hong Kong China

As an audiovisual medium, computer animations require superior image quality and professional soundtrack to lead audiences into their fascinating virtual world. Without sound, the impact of storytelling is reduced or the story is even not under- standable. Despite the importance of sound, most animators are unfamiliar with sound editing software. Limited budget projects such as independent or student works have difficulty hiring sound professionals to create tailor-made soundtrack. Therefore, we need a suitable sound tool to express their individual ideas. In this paper, we propose an approach using schematic for both computer animation and sound. Our approach provides (1) physical simulation on sound through animation parameters and (2) a new channel for animators to add aesthetic values in sound through their experience of using schematics in existing animation software. We demonstrate our idea through several examples, such as Doppler shift, obstacle effect, importance, energetic, and sputtering effect.

关键词： Computer animation Multimedia Digital media Schematics Physical simulation Aesthetic Sound effects Soundtrack

探本溯源——夏含夷《〈周易〉的起源与早期演变》英文版导读

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国际儒学（中英文） 2024年第1期4卷 160-170,189,190页

作者：王嘉宝北京师范大学—香港浸会大学联合国际学院通识教育学院珠海519087

《〈周易〉的起源与早期演变》展示了夏含夷四十年来治易的成果,此书可谓英文学界《周易》古经研究中的里程碑著作。在内容上,此书结合出土材料与传世文献,系统地考辨公元前800—前500年《周易》的起源及其演变。在方法上,夏含夷持有严... 详细信息

《〈周易〉的起源与早期演变》展示了夏含夷四十年来治易的成果,此书可谓英文学界《周易》古经研究中的里程碑著作。在内容上,此书结合出土材料与传世文献,系统地考辨公元前800—前500年《周易》的起源及其演变。在方法上,夏含夷持有严格的经传分观,以《周易》为筮占之书,以《易经》及易学著作为以传释经的哲理智慧之书。此书强调《周易》文本的形成只有放回到“筮占情境”当中,方可探求文本的本来意涵。夏含夷始终从“筮占情境”出发,对《周易》文本形成的诸种细节进行剖析,反对由哲学路径解读《周易》。

关键词：夏含夷卜筮《〈周易〉的起源与早期演变》

Development of An ICR Mouse Bioassay for Toxicity Evaluation in Neurotoxic Poisoning Toxins-Contaminated Shellfish

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Biomedical and Environmental Sciences 2013年第5期26卷 346-364页

作者： wong Chun Kwan HUNG Patricia kam Kai Man Biotoxin Laboratory Microbiology DivisionPublic Health Laboratory Services BranchCentre for Health ProtectionDepartment of HealthShek Kip MeiKowloon

Objective To develop an ICR （female） mouse bioassay （MBA） for toxicity confirmation and evaluation of neurotoxins （brevetoxins）-contaminated shellfish. Methods Brevetoxins （BTX-B） as a causative agent of neurotoxic shellfish poisoning （NSP） under different shellfish matrices were intraperitoneally injected at different doses into mice to study their toxic effects and to differentiate the range of lethal and sublethal dosages. Their sensitivity and specificity were analyzed with 2 competitive ELISA kits for quantitative determination of standard BTX-B and dihydroBTX-B under different shellfish matrix-diluent combinations. Detection rates of MBA and two antibody-based assays for BTX-B from field NSP-positive shellfish samples were compared. Results BTX-B could be detected in shellfish tissues at concentration of 50-400 μg/100 g under shellfish matrix-Tween-saline media, which were appropriate to identify toxic shellfish at or above the regulatory limit （80 μg/100 g shellfish tissues）. The LD 50 identified was 455 g/kg for BTX-B under general shellfish matrices （excluding oyster matrices） dissolved in Tween-saline. The presence of shellfish matrices, of oyster matrices in particular, retarded the occurrence of death and toxicity presentation in mice. Two antibody-based assays, even in the presence of different shellfish matrix-diluent combinations, showed acceptable results in quantifying BTX-B and dihydroBTX-B well below the regulatory limit. Conclusion The two ELISA analyses agree favorably （correlation coefficient, r 0.96; Student＇s t-tests, P〉0.05） with the developed bioassay.

关键词： Antibody-based assay Brevetoxin ICR female mice LD 50 Mouse bioassay Neurotoxic shellfish poisoning

Attenuation of mitochondrial,but not cytosolic,Ca2+ overload reduces myocardial injury induced by ischemia and reperfusion

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Acta Pharmacologica Sinica 2006年第7期27卷 911-918页

作者： Kenneth WL kam James SK SHAM Tlak-ming wong Department of Physiology the University of Hong KongHong Kong SARChina Division of Pulmonary Critical Care Medicine Johns Hopkins School of MedicineBaltimoreMaryland 21224USA

Aim:Attenuation of mitochondrial Ca2+([Ca2+]m),but not cytosolic Ca2+([Ca2+]c),overload improves contractile *** hypothesized that attenuation of[Ca2+]m,but not[Ca2+]c,overload confers cardioprotection against ischemia/reperfusion-induced ***:Infarct size from isolated perfused rat heart,cell viability,and electrically-induced Ca2+transient in isolated rat ventricularmyocytes were *** determined the effects of BAPTA-AM,a Ca2+chelator,at concentrations that abolish the overload of both[Ca2+]cand[Ca2+]m,and ruthenium red,an inhibitor of mitochondrial uniporter of Ca2+transport,atconcentrations that abolish the overload of[Ca2+]m,but not[Ca2+]c,on cardiacinjury induced by ischemia/***:Attenuation of both[Ca2+]mand[Ca2+]cby BAPTA-AM,and attenuation of[Ca2+]m,but not[Ca2+]c,overloadby ruthenium red,reduced the cardiac injury observations,indicating the impor-tance of[Ca2+]min cardioprotection and contractile recovery in response to is-chemia/***:The study has provided unequivocal evidenceusing a cause-effect approach that attenuation of[Ca2+]m,but not[Ca2+]c,over-load is responsible for cardioprotection against ischemia/*** also confirmed the previous observation that attenuation of[Ca2+]m,but not[Ca2+]c,by ruthenium red improves contractile recovery following ischemia/reperfusion.

关键词： ruthenium red BAPTA-AM infarct size electrically-induced Ca2+transient isolated perfused rat heart cardiac myocytes