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Rapid in VitroMultiplication of an Endangered Medicinal Plant Sarpgandha (Rauwolfia serpentina)

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American Journal of Plant Sciences 2012年第4期3卷 437-442页

作者： Subhadra Rani Mallick Ram chandra Jena kailash chandra samal Department of Agricultural Biotechnology Orissa University of Agriculture and Technology Bhubaneswar India Department of Agricultural Biotechnology Orissa University of Agriculture and Technology Bhubaneswar India.

An efficient and reproducible procedure for clonal multiplication through in vitro culture of Rauwolfia serpentina (L.) Benth. (Sarpagandha) is standardized. The different explants like leaf and stem were used for callus induction and regeneration of the complete plantlets. High-frequency callusing was induced in leaf and stem explant of Rauwolfia serpentina on modified Murashige and Skoog (MS) medium supplemented with 2.5 mg/l 2,4-D. Maximum regeneration of shoots from callus (90%) was observed in MS medium supplemented with 0.2 mg/l NAA and 1.5 mg/l BA. However direct regeneration (96%) was recorded best in MS medium supplemented with BAP 2.5 mg/l. The maximum number of shoots per explant (6.5) was also highest in this phyto-hormone combination. Higher induction of root (100%) was observed in MS medium supplemented with NAA 0.5 mg/l. The rooted plantlets were successfully established in the field.

关键词： Rauwolfia serpentine In Vitro Propagation Medicinal Plant Endangered Species

Omics Studies and Systems Biology Perspective towards Abiotic Stress Response in Plants

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American Journal of Plant Sciences 2020年第12期11卷 2172-2194页

作者： Jyoti Prakash Sahoo Laxmipreeya Behera Siddhartha Shankar Sharma Jannila Praveena Suman Kumari Nayak kailash chandra samal Department of Agricultural Biotechnology Odisha University of Agriculture and Technology Bhubaneswar India Department of Genetics and Plant Breeding Uttar Banga Krishi Viswavidyalaya Cooch Behar India Department of Fruit Science and Horticulture Technology Odisha University of Agriculture and Technology Bhubaneswar India Department of Plant Physiology Odisha University of Agriculture and Technology Bhubaneswar India

Plant abiotic stress responses are vital yield-restricting aspect in agriculture. Recent technology in plant biology allows research of such stress responses at a molecular scale in plants. Network analysis provides in-depth knowledge regarding omics information visualisation as it reduces the intrinsic intricacy of such data. The use of integrated functional genomics helps to understand the relationship between the genomic profile and the phenotypic profile in different environmental conditions of an organism. Plants’ responses to abiotic stress are often considered as a complex process. Systems biology approaches allow visualising and understanding how plant life works to overcome abiotic stress. The combination of integrated functional genomics along with bioinformatics will put a hand in additional in-depth research knowledge on stress tolerance to plants by exploiting available genetic information and continuously improving techniques and strategies. Most of the omics technologies are high throughput with very rapid data generation rates and humongous outputs. These technologies have made noticeable contributions to the modern-day improvements in our knowledge of plant biology. So, in this review, omics studies and the system biology approach towards abiotic stress tolerance in plants are highlighted.

关键词： Genomics Proteomics Transcriptomics System Biology Abiotic Stress Plants

The Golden Spice Turmeric (Curcuma longa) and Its Feasible Benefits in Prospering Human Health—A Review

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American Journal of Plant Sciences 2021年第3期12卷 455-475页

作者： Jyoti Prakash Sahoo Laxmipreeya Behera Jannila Praveena Shraddha Sawant Ankita Mishra Siddhartha Shankar Sharma Lipi Ghosh Ambika Prasad Mishra Asit Ranjan Sahoo Pranay Pradhan Subhasmita Sahu Ankit Moharana kailash chandra samal Department of Agricultural Biotechnology Odisha University of Agriculture and Technology Bhubaneswar India Department of Fruit Science and Horticulture Technology Odisha University of Agriculture and Technology Bhubaneswar India Department of Plant Pathology Odisha University of Agriculture and Technology Bhubaneswar India Department of Plant Breeding and Genetics Odisha University of Agriculture and Technology Bhubaneswar India Department of Genetics and Plant Breeding and Seed Science & Technology Centurion University of Technology and Management Parlakhemundi India School of Development Studies Tata Institute of Social Sciences Mumbai India Department of Soil Science and Agricultural Chemistry Faculty of Agriculture Sri Sri University Cuttack India Department of Nematology Odisha University of Agriculture and Technology Bhubaneswar India Department of Floriculture and Landscaping Odisha University of Agriculture and Technology Bhubaneswar India Department of Seed Science and Technology Odisha University of Agriculture and Technology Bhubaneswar India

From the evolution of the mankind, Turmeric has been used in conventional medication. India is in lead for producing, marketing and exporting the Turmeric and its value added products. Curcuma longa (Turmeric) is an Indian rhizomatous medicinal herb from the Zingiberaceae family that is common and widely available across the globe. The components of Turmeric are curcumin, demethoxycurcumin and bisdemethoxycurcumin and these are collectively known as curcuminoids. Curcumin, the active ingredient of Turmeric is generally investigated by the scientific community for its wide range of antioxidant activity, anti-Inflammatory properties and anti-cancer activity, anti-metabolic syndrome activities, neuroprotective activity, antimicrobial effects, anti-arthritis effects, anti-viral effects, anti-asthma and anti-diabetic effects, anti-obesity, cardio and liver toxicity protection activity, anti-depression and anxiety activities. Turmeric has been widely used as a typical household treatment for cough, sore throat, respiratory ailments and could be an effective immunity booster against SARS-CoV-2 therapy during the ongoing pandemic situation. Safety evaluation studies indicate that both turmeric and curcumin are well tolerated at a very high dose without any toxic effects. Thus, turmeric and its constituents have the potential for the development of modern medicine for the treatment of various diseases. So in this review, we describe the various metabolic roles of curcumin and activities for the benefit of human health.

关键词： Turmeric Plant Extracts Curcumin Medicinal Properties SARS-CoV-2 Immunity

The Determination of Heritability in palatal Rugae Pattern among Parents and their Off-Springs in Western India-A Cross-sectional Study

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Journal of Forensic Science and Medicine 2024年第1期10卷 42-46页

作者： Aishwarya Bhatnagar Sugandha Arya Jyothi S Kumar Aishwarya Arya kailash chandra Morya Sudhir Baroopal Department of Oral Medicine and Radiology Vyas Dental College and HospitalJodhpurRajasthanIndia Department of Conservative and Endodontics Vyas Dental College and HospitalJodhpurRajasthanIndia

Background:Palatal rugae are asymmetrical elevations present on hard palate and are significant due to their uniqueness,stability and thus,aid in forensic ***:The objective is to determine palatal rugae morphology between parents and their offsprings and to determine their genetic *** and Methods:This randomized controlled cross-sectional single-blinded study was conducted on 50 families(randomly selected)who visited Vyas Dental College and Hospital.A total of 150 maxillary study models were examined and rugae were delineated and shape,size,and pattern of rugae were ***:Weak but significant correlation was seen in the nonspecific and angular type of palatal rugae between child and mother and child and father as P<*** with either or both of their parent was seen for curved,wavy,divergent,straight,horseshoe,angular,and nonspecific type of rugae with the higher%of wavy pattern matching(52%with mother and 54%with father).A considerable number of samples showed high heritability of primary and secondary rugae from both parents,but more predominantly to their mothers(branched,nonspecific,and straight).Conclusion:Both environmental and genetic factors can influence the rugae ***,studies should be conducted on a larger sample size to get more significant results.

关键词： Forensic identification heredity palatal rugae uniqueness

Incidence and management of white grub, Schizonycha ruficollis on seedlings of teak （Tectona grandis Linn, f,）

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Insect Science 2007年第5期14卷 411-418页

作者： NITIN KULKARNI kailash chandra PRAFULLA NARAHAR WAGH kailash chandra JOSHI RAM BHAJAN SINGH Forest Entomology Division Tropical Forest Research Institute Jabalpur (M. P.) Central Regional Station Zoological Survey of India Vijay Nagar Jabalpur (M. P.) Forest Development Corporation of Maharashtra Nagpur India

In investigation was conducted annually from 2002 to 2005 between the second week of May and the end of September on the incidence of scarab beetle, Schizonycha ruficollis （Fabricius） in a teak （Tectona grandis L.f.） nursery at Ramdongari, Nagpur, India. The immature stages, that is white grubs of the scarab beetle damaged 14%-52% of teak seedlings in the nursery beds, whereas the adult of the species, that is scarab beetles, were recorded on three new host trees （ Ziziphus xylopyra, Acacia catechu and A. leucophloea） in addition to the reported hosts such as ziziphus （Z. jujuba and Z. mauritiana）. Beetles of the S. ruficollis started emerging from the nursery beds just after the pre-monsoon showers and continued for 10-18 days. While beetle of S. ruficollis defoliated tree hosts available in the surroundings, the immature stages of the beetle caused major damage by feeding on root systerfls of teak seedlings with symptoms of wilting and mortality in forest nursery beds. The result of chemical and biological treatments for the management of grubs of S. ruficollis in nursery beds indicated phorate 10% granular at the rate of 20 g/m^2 and chlorpyriphos 20% emulsifiable concentrate at the rate of 5.0 mL/m^2 to be effective. Grubs of S. ruficollis have not been recorded previously as a major pest of teak （T. grandis）. This is also the first report on occurrence of S. ruficollis adults on tree hosts such as Z. xylopyra, A. catechu and A. leucophloea. White grubs being pests of economic importance and the toughest insects to manage, information given in this paper on incidence, pest status and management of Schizonycha ruficollis is of importance to researchers and forest nursery managers.

关键词： bioecology Coleoptera field incidence forest nursery insect pest management,insecticides Melolonthinae Scarabaeidae

Comparative Electrocatalytic Oxygen Evolution Reaction Studies of Spinel NiFe_(2)O_(4) and Its Nanocarbon Hybrids

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Transactions of Tianjin University 2022年第1期28卷 80-88页

作者： Pratik V.Shinde Rutuparna samal chandra Sekhar Rout Centre for Nano and Material Sciences Jain UniversityBengaluruKarnataka 562112India

Electrocatalytic oxygen evolution reaction(OER)is one of the crucial reactions for converting renewable electricity into chemical fuel in the form of *** date,there is still a challenge in designing ideal cost-effective OER catalysts with excellent activity and robust *** hybridization of transition metal oxides and carbonaceous materials is one of the most effective and promising strategies to develop high-performance ***,this work synthesized hybrids of NiFe_2 O_4 spinel materials with two-dimensional(2D)graphene oxide and one-dimensional(1 D)carbon nanotubes using a facile solvothermal *** activities of NiFe_(2)O_(4) with 2 D graphene oxide toward OER were realized to be superior even to the 1 D carbon nanotube-based electro catalyst in terms of overpotential to reach a current density of10 mA/cm^(2) as well as Tafel *** NiFe_(2)O_(4) with 2 D graphene oxide hybrid exhibits good stability with an overpotential of 327 mV at a current density of 10 mA/cm^(2) and a Tafel slope of 103 mV/*** high performance of NiFe_(2)O_(4) with 2 D graphene oxide is mainly attributed to its unique morphology,more exposed active sites,and a porous structure with a high surface ***,an approach of hybridizing a metal oxide with a carbonaceous material offers an attractive platform for developing an efficient electrocatalyst for water electrochemistry applications.

关键词： Oxygen evolution reaction Metal oxide Graphene oxide Carbon nanotubes Stability

White grubs, Holotrichia rustica and Holotrichia mucida （Coleoptera： Scarabaeidae） as pests of teak （Tectona grandis L. f.） seedlings

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Insect Science 2009年第6期16卷 519-525页

作者： Nitin Kulkarni Sanjay Paunikar kailash chandra Joshi John Rogers Tropical Forest Research Institute Jabalpur India Research Connections and Consulting P.O. Box 350 Toowong Queensland 4066 Australia

Field observations were carried out between 2002 and 2005 on bio-ecology of white grubs infesting teak （Tectona grandis Linn. f.） seedlings at Ramdongari Forest Nursery, Forest Development Corporation of Maharashtra, Nagpur in central India. The study collected the data required for an integrated pest management strategy against the white grubs. The species damaging teak seedlings were Holotrichia rustica （Burmeister） and H. mucida Gyllenhal. The beetles （adults） of both these species began to emerge just after the pre-monsoon showers and when the relative humidity had risen rapidly over several days, but before the first monsoon rains. While the adults fed and mated on naturally growing bushes of Ziziphus jujuba, Z mauritiana, Z. xylopyra, Acacia leucophloea and A. catechu immediately after the emergence from the teak beds in the nursery, the immature stages （grubs） of both species fed on roots of the teak seedling, leading to plant death. This is the first report of （i） damage caused to teak seedlings by grubs ofH. rustica and H. mucida, and （ii） of *** and Z. mauritiana being hosts for the beetles ofH. mucida and H, rustica. It is also the first detailed report on the field bio-ecology of the H. rustica and H. mucida as major pests ofteak. White grubs are among the toughest-to-manage pests of economic importance and information presented here on the pest incidence and damage ofH. rustica and H. mucida is important to researchers and nursery field managers for the management of these pests on teak.

关键词： adult emergence chafer beetles environmental factors incidence Indian scarabs insect pests Melolonthinae population density Ziziphus

An Acoustic Analysis of Fluctuations for Inter-and Intra-Speaker Variability in Speech Sounds

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Journal of Forensic Science and Medicine 2023年第1期9卷 38-43页

作者： Jasdeep Kaur kailash chandra Juglan Kush Sharma Vishal Sharma Department of Physics School of Chemical Engineering and Physical Sciences Lovely Professional UniversityPhagwaraPunjab Data Manager School of Community Medicine and Public HealthPostgraduate Institute of Medical Education and ResearchChandigarh Institute of Forensic Science and Criminology Panjab UniversityChandigarhIndia

Background:Variation in the speech of speakers is a crucial issue for the forensic *** main reason behind incorrect speaker identification is greater intra-speaker *** the forensic state of play,a lot of research has been carried out on speaker *** inter variations and intra fluctuations in speakers for the Punjabi language is still a grey *** and Objectives:Our aim is to study acoustic analysis of fluctuations for inter and intra speaker variability in speech *** our study,we will consider Punjabi vowel with *** Statistical methods will be applied to analyze the data;firstly,the Shapiro-Wilk test will be checked for normality and then Levene’s Test to assess the equality of *** and Method:Five vowels were selected with different *** were combined to make meaningful *** these meaningful words were embedded in *** speakers participated *** are students of A.S College at Khanna in *** individuals were aged between 20-22 years with no hearing or speech *** voice samples were recorded with help of good quality microphone and by Goldwave software in the sound proof *** were introduced directly into PRAAT software by the use of a Sony microphone and with sampling rate of 44100 Hz *** Analysis has been done with help of Goldwave software in form of *** and Conclusion:Each formant shows a different value for inter variations and inter speaker fluctuations.F1 and F2 shows lesser speaker variation than the high-frequency region in F3 and F4,so we can say that in comparison with the lower part,high-frequency regions are more *** assumptions for TWO-WAY ANOVA is violated and hence,we have used the non-parametric Friedman Test and performed its Post hoc *** Posthoc analysis,we can say that F1 and F2(p>0.05)and F2 and F3(p>0.05)gave the same type of ***,from the results of these st

关键词： Forensic voice identification Friedman test intervariations and intrafluctuations Punjabi

Cation Distribution in Lithium Ferrite(LiFe_(5)O_(8))Prepared via Aerosol Route

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Journal of Electromagnetic Analysis and Applications 2010年第1期2卷 51-55页

作者： Sonal Singhal kailash chandra Department of Chemistry Panjab UniversityChandigarhIndia Institute Instrumentation Centre Indian Institute of TechnologyRoorkeeIndia

Nano size lithium ferrite was prepared through aerosol route and characterized using TEM, XRD, magnetic measurements and M?ssbauer spectroscopy. The particle size of as obtained samples were found to be ~10 nm through TEM, that increases upto ~80 nm on annealing at 1200 oC. The unit cell parameter ‘a’ calculated using XRD, confirms the formation of ?-LiFe5O8. Room temperature M?ssbauer spectra of as obtained sample of all the ferrites exhibited broad doublet suggesting super paramagnetic nature. This doublet further resolved into two doublets and assigned to the surface region atoms and internal region atoms of the particles. The annealed samples (1200 oC) show broad sextets, which were fitted with two sextets indicating different local environment of both tetrahedrally and octahedrally coordinated Fe-cation. Cation distribution obtained from the X-ray, magnetic and M?ssbauer data confirms that the three fifth of the iron atom goes in to the octahedral site.

关键词： Nano Particles Saturation Magnetization Coercivity X-Ray Diffraction Mossbauer Spectra