检索结果-南通市图书馆

Application of laparoscopic total mesorectal excision combined with sphincter-preserving surgery in low or ultralow rectal cancer

引用

Journal of Innovative Optical Health Sciences 2016年第5期9卷 8-14页

作者： Yan Liu Xiao-Ming Lu Yan-Feng Niu kai-xiong tao Guo-Bin Wang Department of Gastrointestinal Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 P.R.China

This study aimed to investigate the influence of laparoscopic total mesorectal excision combined with sphincter-preserving surgery on the postoperative defecation function,urinary function and sexual function in low or ultralow rectal cancer.A retrospective study was undertaken on 107 patients(65 laparoscopic resection and 42 laparoctomic resection)with rectal cancer undergoing laparoscopic or open laparoscopic total mesorectal excision combined with sph incter-preserving surgery from April 2009 to April *** quality of life outcomes of all patients,including defection,urinary and sexual function,were assessed at 6,12 and 24 months after *** quality of life index(GQOLI)was used to evaluate the fecal incont inence and bowel *** and sexual functions were studied by means of questionnaires on the basis of the international prostatic symptom score(IPSS)and international index of erectile function(IEF),*** laparoscopic surgery group,there were two cases of anastomotic leakage,three cases of anast omotic strict ure,seven cases of local recurrence,ten cases of hepatic metastasis and five cases of lung *** satisfaction rates of patients about their defe-cation function reached 60.3%(35/58,84.5%(49/58)and 913%(53/58)at6,12,and 24 months follow-up,*** assessment after one year showed that the overall incidence of uri-nary dysfunction was 10.7%(7/65);Among male patients,18.4%(7/36)sufered from erectile dysfunction and 27.8%(10/36)sufered from ejaculatory dysfunction;65.5%(19/29)female patients investigated were satisfied with their postoperative sexual *** open surgery group,there were two cases of anastomotic leakage,two cases of anastomotic stricture,nine cases of local recurrence,ten cases of hepatic metastasis and seven cases of kung *** satisfaction rates of patients about their defecation function were 56.4%(22/39),82.1%(32/39)and 94.8%(37/39)at 6,12,and 24 months follow-up,resp

关键词： Rectal cancer laparoscope sphincter-preserving surgery quality of life.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enhanced Chemotherapy Sensitivity of Human Colon Cancer Cells to 5-Fluorouracil by siRNA Recombinant Expression Vector Targeting Survivin Gene

引用

Chinese Medical Sciences Journal 2009年第2期24卷 97-101页

作者： Ming Cai Guo-bin wang kai-xiong tao Chang-xue Cai Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Pathogenic Biology School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective To investigate the effects of small interfering RNA （siRNA） recombinant expression vector targeting survivin gene on chemotherapy sensitivity of human colon cancer cells to 5-fluorouracil. Methods siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cell lines LOVO. After 48 hours of transfection, cells were harvested for analysis of survivin mRNA and protein expressions using RT-PCR and Western blot. In addition, after human colon cancer cell lines were treated with Survivin siRNA and/or 5-fluorouracil, MTT assay and flow cytometry were used to analyze cell proliferation and apoptosis. Results Restriction endonuclease analysis confirmed that siRNA recombinant expression vector targeting survivin gene was successfully constructed. Inhibitory ratios of survivin mRNA and protein expressions by Survivin siRNA were 36.33% and 44.65%, respectively. Survivin siRNA combined with 5-fluorouracil significantly increased the cell proliferation inhibitory ratio and apoptosis ratio compared with 5-fluorouracil treatin~ alone （P〈0.05）. Conclusion The siRNA recombinant expression vector targeting survivin gene can inhibit the expression of survivin gene, and enhance chemotherapy sensitivity of human colon cancer cells to 5- fluorouracil.

关键词： survivin RNA interference gene therapy chemotherapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a magnetite-gene complex for gene transfection

引用

Frontiers of Medicine 2010年第2期4卷 241-246页

作者： Jian XIN Yan LUO Ze-Feng XIA kai-xiong tao kai-Lin CAI Gao-xiong HAN Xiao-Ming SHUAI Ji-Liang WANG Han-Song DU Guo-Bin WANG Department of Laparoscopic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Ophthalmology Wuhan No.1 HospitalWuhan 430022China

The key to successful gene therapy is toﬁnd a suitable method and carrier for transfection to allow a gene to be transferred into a cell and integrated into the target *** aim of this study was to determine whether biomagnetic material could be combined with the nucleic acid for gene ***-coated iron oxide nanoparticles(DCIONPs)were prepared and mixed with the plasmid pGenesil-1 containing the test gene,which expresses enhanced greenﬂuorescent protein(eGFP).PGenesil-1 empty vector was used as a *** binding ability was assessed by electrophoresis of the DNA on agarose gels and quantiﬁcation using BANDS-CAN *** different gene carriers,Lipofecta-mine 2000,Sofast,and DCIONPs,the large intestine cancer(Lovo)cell line was transfected in vitro with or without a magneticﬁ*** expression of eGFP was observed byﬂuorescence microscopy,and the transfection efﬁciency was *** results showed there was a rapid increase in combining rate when the quality ratio of DCIONPs and pGenesil-1 ascended from 1∶1 to 5∶***,the combining rate increased less rapidly as the quality ratio continued *** expression of eGFP showed that the early transfection rate could be improved by applying a magneticﬁ*** conclusion,the DCIONPs we synthesized are able to carry plasmid DNA and enhance the early transfection efﬁciency when using a magneticﬁeld.

关键词： nanoparticle magnetite gene therapy mag-netofection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Risk Factors of Anastomotic Leakage After Anterior Resection for Rectal Cancer Patients

引用

Current Medical Science 2022年第6期42卷 1256-1266页

作者： Xiang-nan YU Lu-ming XU Ya-wen BIN Ye YUAN Shao-bo TIAN Bo CAI kai-xiong tao Lin WANG Guo-bin WANG Zheng WANG Department of Gastrointestinal Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Research Center for Tissue Engineering and Regenerative Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Clinical Laboratory Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Objective Anastomotic leakage(AL)is one of the serious complications after anterior resection for rectal *** stoma(DS)is one of the most widely used approaches to prevent it;however,the effect of DS on the occurrence of AL remains *** study aimed to investigate risk factors of AL and assess the effect of DS after anterior resection for rectal cancer *** A retrospective analysis was conducted for the data of 1840 patients who underwent anterior resection for rectal cancer from January 2014 to December *** The results showed the overall AL incidence was 7.5%.Multivariate analyses revealed that males[odds ratio(OR)1.562]and T3–T4 stage(OR 1.729)were independent risk factors for all *** propensity score matching analysis,the AL incidence was 14.1%in the group with no DS and 6.4%in the DS group(P<0.001).The clinical AL(grade B+grade C)incidence was 12.4%in no DS group and 4.6%in the DS group(P<0.001).Conclusion The study suggested that males and T3–T4 stage were independent risk factors of *** addition,DS could reduce the rate of symptomatic AL.

关键词： anastomotic leakage risk factor defunctioning stoma propensity score matching

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Strategies and recommendations for the management of gastrointestinal surgery during the COVID-19 pandemic:experience shared by Chinese surgeons

引用

Gastroenterology Report 2020年第3期8卷 167-174,I0001页

作者： Jia Ke Nan Lan Ting Wang Jin-Jie Wu Zhen He Xiao-Sheng He kai-xiong tao Qun Qian Ping-Hong Zhou Guo-Xin Li Min-Hua Zheng Zhong-tao Zhang Jia-Fu Ji Ping Lan Department of Colorectal Surgery The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdongP.R.China Guangdong Institute of Gastroenterology Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor DiseasesGuangzhouGuangdongP.R.China Department of Gastrointestinal Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Department of General Surgery Zhongnan Hospital of Wuhan UniversityWuhanHubeiP.R.China Endoscopy Center Zhongshan HospitalFudan UniveristyShanghaiP.R.China Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Department of Gastrointestinal Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiP.R.China Department of General Surgery Beijing Friendship HospitalCapital Medical UniversityNational Clinical Research Center for Digestive DiseasesBeijingP.R.China Department of Gastrointestinal Cancer Center Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education)Peking University Cancer Hospital&InstituteBeijingP.R.China

Novel coronavirus disease-2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is an ongoing public-health pandemic *** SARS-CoV-2 has been known to spread primarily through respiratory droplets,recent evidence also supports fecal/oral as an additional route of transmission,raising concerns over gastrointestinal(GI)transmission of the ***,we,as the front-line Chinese GI surgeons,would like to share our experience and lessons in the combat against *** is essential to create science-based,rational,and practical strategies during the outbreak of ***,we provide multi-institutional consensus on minimizing disease transmission while continuing to provide care from all aspects for patients in GI surgery,including outpatient clinics,inpatient units,gastrointestinal endoscopy centers,and adjustments in perioperative *** experiences and recommendations are worth sharing and may help to establish specific infection-control and outcome measures.

关键词： gastroenterological surgery novel coronavirus disease-2019 perioperative care severe acute respiratory syndrome coronavirus 2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Performance of risk stratification systems for gastrointestinal stromal tumors: A multicenter study

引用

World Journal of Gastroenterology 2019年第10期25卷 1238-1247页

作者： tao Chen Liang-Ying Ye Xing-Yu Feng Hai-Bo Qiu Peng Zhang Yi-Xin Luo Li-Yi Yuan Xin-Hua Chen Yan-Feng Hu Hao Liu Yong Li kai-xiong tao Jiang Yu Guo-Xin Li Department of General Surgery Nanfang HospitalSouthern Medical University Department of Gastroenterology Nanfang HospitalSouthern Medical University Department of General Surgery Guangdong General HospitalGuangdong Academy of Medical Sciences Department of Gastric Surgery Sun Yat-sen University Cancer CenterSun Yatsen University Department of General Surgery Wuhan Union HospitalHuazhong University of Science and Technology

BACKGROUND Gastrointestinal stromal tumors(GISTs) are the most common mesenchymal tumor type in the gastrointestinal system. Presently, various classification systems to prognosticate GISTs have been *** To evaluate the application value of four different risk stratification systems for *** Patients who were diagnosed with GISTs and underwent surgical resection at four hospitals from 1998 to 2015 were identified from a database. Risk of recurrence was stratified by the modified National Institute of Health(NIH)criteria, the Armed Forces Institute of Pathology(AFIP) criteria, the Memorial Sloan Kettering Cancer Center(MSKCC) prognostic nomogram, and the contour maps. Receiver operating characteristic(ROC) curves were established to compare the four abovementioned risk stratification systems based on the area under the curve(AUC).RESULTS A total of 1303 patients were included in the study. The mean age of the patients was 55.77 ± 13.70 yr; 52.3% of the patients were male. The mean follow-up period was 64.91 ± 35.79 mo. Approximately 67.0% the tumors were located in the stomach, and 59.5% were smaller than 5 cm; 67.3% of the patients had a mitotic count ≤ 5/50 high-power fields(HPFs). Thirty-four tumors ruptured before and during surgery. Univariate analysis demonstrated that tumor size > 5 cm(P 5/50 HPFs(P < 0.05), non-gastric location(P < 0.05), and tumor rupture(P < 0.05) were significantly associated with increased recurrence rates. According to the ROC curve, the AFIP criteria showed the largest AUC(0.754).CONCLUSION According to our data, the AFIP criteria were associated with a larger AUC than the NIH modified criteria, the MSKCC nomogram, and the contour maps, which might indicate that the AFIP criteria have better accuracy to support therapeutic decision-making for patients with GISTs.

关键词： Gastrointestinal stromal tumors Risk stratification Prognosis Modified National Institute of Health criteria Armed Forces Institute of Pathology criteria Memorial Sloan Kettering Cancer Center prognostic nomogram Contour maps Gastrointestinal tumors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinicopathological Characteristics and Prognosis of Hepatoid Adenocarcinoma of the Stomach:Evaluation of a Pooled Case Series

引用

Current Medical Science 2018年第6期38卷 1054-1061页

作者： Xiang-yu ZENG Yu-ping YIN Hua XIAO Peng ZHANG Jun HE Wei-zhen LIU Jin-bo GAO Xiao-ming SHUAI Guo-bin WANG Xiu-li WU kai-xiong tao Department of Gastrointestinal Surgery Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Gastroduodenal and Pancreatic Surgery Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine Central South UniversityChangsha 410013China Department of Pathology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Hepatoid adenocarcinoma of the stomach (HAS)is an extremely rare and unique gastric *** present study aimed to examine the relevance of the clinicopathological characteristics of HAS with patient *** retrospectively reviewed clinical data of 34 HAS patients treated at our institution between January 2010 and December 2016,as well as 294 cases reported prior to 2017 in research *** these patients,45.6%(115/252)had lesions in the gastric antrum and 77.0%(235/305)were *** levels of serum alpha-fetoprotein (AFP)were detected in most patients(75/93,80.6%).Vascular invasion(199/286,69.6%),lymph node metastasis (222/283,78.4%),and preoperative distant metastasis (121/328,36.9%)were commonly *** 5-year disease-free survival (DFS)and disease-specific survival (DSS) were 20.7%and 29.2%,*** and DSS of patients receiving neoadjuvant therapy were significantly higher than those of patients receiving postoperative adjuvant therapy [DFS:P<0.001, hazard ratio (HR)=-1.831,95%confidence interval (CI):0.060-0.429;DSS:P<0.001,HR=-2.185, 95%CI:0.032-0.401].In conclusion,HAS exhibits distinct clinicopathological characteristics and a strikingly worse prognosis when compared with common gastric *** surgery,early pTNM stage,and adjuvant therapy may predict a more favorable *** therapy is strongly recommended for patients with lymph node metastasis or/and preoperative distant metastasis.

关键词： hepatoid adenocarcinoma stomach clinicopathological characteristics adjuvant therapy neoadjuvant therapy prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

hTERT表达的表观遗传调控研究进展

引用

世界华人消化杂志 2010年第10期18卷 1026-1031页

作者：李伟陶凯雄华中科技大学同济医学院附属协和医院普外科湖北省武汉市430022

端粒酶逆转录酶(hTERT)是端粒酶的限速成分,hTERT基因的表达调控对端粒酶表达活性调节起决定性作用.表观遗传学是在不改变DNA序列情况下,通过DNA和组蛋白修饰或者非编码RNA影响基因表达,这种改变具有可遗传性,其在基因表达调控中具有重... 详细信息

端粒酶逆转录酶(hTERT)是端粒酶的限速成分,hTERT基因的表达调控对端粒酶表达活性调节起决定性作用.表观遗传学是在不改变DNA序列情况下,通过DNA和组蛋白修饰或者非编码RNA影响基因表达,这种改变具有可遗传性,其在基因表达调控中具有重要作用同样,表观遗传学可通过DNA甲基化,组蛋白乙酰化与甲基化和非编码RNA来调节hTERT基因表达,是端粒酶活性调节的重要机制.

关键词：端粒酶逆转录酶表观遗传学甲基化乙酰化非编码RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

GATIS score for predicting the prognosis of rectal neuroendocrine neoplasms:A Chinese multicenter study of 12-year experience

引用

World Journal of Gastroenterology 2024年第28期30卷 3403-3417页

作者： Xin-Yu Zeng Ming Zhong Guo-Le Lin Cheng-Guo Li Wei-Zhong Jiang Wei Zhang Li-Jian Xia Mao-Jun Di Hong-Xue Wu Xiao-Feng Liao Yue-Ming Sun Min-Hao Yu kai-xiong tao Yong Li Rui Zhang Peng Zhang Department of Gastrointestinal Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430074Hubei ProvinceChina Department of Gastrointestinal Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of General Surgery Peking Union Medical College HospitalPeking Union Medical CollegeBeijing 100730China Department of Colorectal Surgery Fujian Medical UniversityFuzhou 350401Fujian ProvinceChina Department of Colorectal Surgery Changhai HospitalShanghai 200433China Department of Gastrointestinal Surgery The First Affiliated Hospital of Shandong First Medical UniversityJinan 250118Shandong ProvinceChina Department of Gastrointestinal Surgery Taihe HospitalHubei University of MedicineShiyan 442099Hubei ProvinceChina Department of Gastrointestinal Surgery I Section Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina Department of General Surgery Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021Hubei ProvinceChina Department of Colorectal Surgery Jiangsu Province HospitalNanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Gastrointestinal Surgery School of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of General Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080Guangdong ProvinceChina Department of Colorectal Surgery Liaoning Cancer HospitalShenyang 110042Liaoning ProvinceChina

BACKGROUND There is currently a shortage of accurate,efficient,and precise predictive instruments for rectal neuroendocrine neoplasms(NENs).AIM To develop a predictive model for individuals with rectal NENs(R-NENs)using data from a large *** Data from patients with primary R-NENs were retrospectively collected from 17 large-scale referral medical centers in *** forest and Cox proportional hazard models were used to identify the risk factors for overall survival and progression-free survival,and two nomograms were *** A total of 1408 patients with R-NENs were *** grade,T stage,tumor size,age,and a prognostic nutritional index were important risk factors for *** GATIS score was calculated based on these five *** overall survival prediction,the respective C-indexes in the training set were 0.915(95%confidence interval:0.866-0.964)for overall survival prediction and 0.908(95%confidence interval:0.872-0.944)for progression-free survival *** to decision curve analysis,net benefit of the GATIS score was higher than that of a single *** time-dependent area under the receiver operating characteristic curve showed that the predictive power of the GATIS score was higher than that of the TNM stage and pathological grade at all time *** The GATIS score had a good predictive effect on the prognosis of patients with R-NENs,with efficacy superior to that of the World Health Organization grade and TNM stage.

关键词： Rectal neuroendocrine neoplasm Nomogram Random forest Prognosis Overall survival Progression-free survival

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ERβ和VEGF在大肠癌中的表达及临床意义

引用

世界华人消化杂志 2007年第24期15卷 2652-2656页

作者：李建文陶凯雄蔡开琳华中科技大学同济医学院附属协和医院普通外科湖北省武汉市430022

目的:探讨雌激素受体β(ERβ)、血管内皮生长因子(VEGF)在大肠癌组织中的表达以及与大肠癌分化程度之间的关系.方法:应用免疫组织化学SP法,检测45例大肠癌中ERβ,VEGF的表达,并以10例正常大肠黏膜组织作对照,分析大肠癌组织中ERB与VEGF... 详细信息

目的:探讨雌激素受体β(ERβ)、血管内皮生长因子(VEGF)在大肠癌组织中的表达以及与大肠癌分化程度之间的关系.方法:应用免疫组织化学SP法,检测45例大肠癌中ERβ,VEGF的表达,并以10例正常大肠黏膜组织作对照,分析大肠癌组织中ERB与VEGF的表达与大肠癌的关系.结果:45例大肠癌组织中,ERβ阳性23例(51.11%),VEGF阳性33例(73.33%).ERβ和VEGF的表达与肿瘤的分化程度有关(P= 0.024,P=0.005),而与肿瘤的临床分期无关(P=0.289,P=0.173).ERB阳性组中,VEGF的表达明显低于ERβ阴性组(56.52% vs 90.90%,P<0.05).结论:ERβ的阳性表达与大肠癌的发生、发展和预后密切相关,可联合VEGF作为有价值的肿瘤标记和预后指标.

关键词：大肠癌雌激素受体β 血管内皮生长因子免疫组化

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：