Development of a magnetite-gene complex for gene transfection
为基因 transfection 的磁铁矿基因建筑群的开发作者机构：Department of Laparoscopic SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of OphthalmologyWuhan No.1 HospitalWuhan 430022China
出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)
年 卷 期：2010年第4卷第2期
页 面：241-246页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：nanoparticle magnetite gene therapy mag-netofection
摘 要：The key to successful gene therapy is tofind a suitable method and carrier for transfection to allow a gene to be transferred into a cell and integrated into the target *** aim of this study was to determine whether biomagnetic material could be combined with the nucleic acid for gene ***-coated iron oxide nanoparticles(DCIONPs)were prepared and mixed with the plasmid pGenesil-1 containing the test gene,which expresses enhanced greenfluorescent protein(eGFP).PGenesil-1 empty vector was used as a *** binding ability was assessed by electrophoresis of the DNA on agarose gels and quantification using BANDS-CAN *** different gene carriers,Lipofecta-mine 2000,Sofast,and DCIONPs,the large intestine cancer(Lovo)cell line was transfected in vitro with or without a magneticfi*** expression of eGFP was observed byfluorescence microscopy,and the transfection efficiency was *** results showed there was a rapid increase in combining rate when the quality ratio of DCIONPs and pGenesil-1 ascended from 1∶1 to 5∶***,the combining rate increased less rapidly as the quality ratio continued *** expression of eGFP showed that the early transfection rate could be improved by applying a magneticfi*** conclusion,the DCIONPs we synthesized are able to carry plasmid DNA and enhance the early transfection efficiency when using a magneticfield.
维普期刊数据库