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Ethanol Increases Mechanical Pain Sensitivity in Rats via Activation of GABA_A Receptors in Medial Prefrontal Cortex

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Neuroscience Bulletin 2016年第5期32卷 433-444页

作者： kai-wen geng Ting He Rui-Rui Wang Chun-Li Li wen-Jun Luo Fang-Fang Wu Yan Wang Zhen Li Yun-Fei Lu Su-Min Guan Jun Chen Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders Tangdu Hospital The Fourth Military Medical University Xi'an 710038 China Key Laboratory of Brain Stress and Behavior People's Liberation Army Xi'an 710038 China Beijing Institute for Brain Disorders Beijing 100069 China School of Stomatology The Fourth Military Medical University Xi'an 710032 China

Ethanol is widely known for its ability to cause dramatic changes in emotion, social cognition, and behavior following systemic administration in *** neuroimaging studies suggest that alcohol dependence and chronic pain may share common mechanisms through amygdala-medial prefrontal cortex（m PFC） interactions. However, whether acute administration of ethanol in the m PFC can modulate pain perception is *** we showed that bilateral microinjections of ethanol into the prelimbic and infralimbic areas of the m PFC lowered the bilateral mechanical pain threshold for 48 h without influencing thermal pain sensitivity in adult ***, bilateral microinjections of artificial cerebrospinal fluid into the m PFC or bilateral microinjections of ethanol into the dorsolateral PFC（also termed as motor cortex area 1 in Paxinos and Watson＇s atlas of The Rat Brain. Elsevier Academic Press, Amsterdam, 2005） failed to do so, suggesting regional selectivity of the effects of ethanol. Moreover, bilateral microinjections of ethanol didnot change the expression of either pro-apoptotic（caspase-3 and Bax） or anti-apoptotic（Bcl-2） proteins, suggesting that the dose was safe and validating the method used in the current study. To determine whether c-aminobutyric acid A（GABA_A） receptors are involved in mediating the ethanol effects, muscimol, a selective GABA_Areceptor agonist, or bicuculline, a selective GABA_A receptor antagonist, was administered alone or co-administered with ethanol through the same route into the bilateral m PFC. The results showed that muscimol mimicked the effects of ethanol while bicuculline completely reversed the effects of ethanol and muscimol. In conclusion, ethanol increases mechanical pain sensitivity through activation of GABA_A receptors in the m PFC of rats.

关键词： Ethanol mPFC Pain perception GABAA receptor Empathy

Image-Forming Visual Basis of Empathy for Pain in Mice

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Neuroscience Bulletin 2020年第12期36卷 1563-1569页

作者： kai-wen geng Rui Du Na Wei Chun-Li Li Yan Wang Wei Sun Tao Chen Dong-Yu Wei Yang Yu Ting He wen-Jun Luo Rui-Rui Wang Zhou-Feng Chen Jun Chen Institute for Biomedical Sciences of Pain Tangdu HospitalThe Fourth Military Medical UniversityXi'an 710038China Key Laboratory of Brain Stress and Behavior People's Liberation ArmyXi'an 710038China Center of Clinical Aerospace Medicine The Fourth Military Medical UniversityXi'an 710032China Center for the Study of Itch Washington University School of MedicineSt.LouisMO 63110USA Department of Anesthesiology Washington University School of MedicineSt.LouisMO 63110USA Department of Psychiatry Washington University School of MedicineSt.LouisMO 63110USA Department of Developmental Biology Washington University School of MedicineSt.LouisMO 63110USA

Dear *** for distress refers to the highly evolutionarilyconserved ability of humans and other social animals to feel,recognize,and understand others’painful conditions(pain,fear,and catastrophe)[1,2]and even benefit others by releasing distress through sharing,caring,and cooperation[1].In the past decade,several types of lower empathic response have been gradually identified and characterized in laboratory rodents(rats and mice),referred to as empathic contagious pain[3-5],observational fear learning[2],and contagious itch[6].

关键词： distress catastrophe Visual

Stress relaxation properties of calcium silicate hydrate:a molecular dynamics study

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第2期25卷 97-115页

作者： Zhicheng geng Shengwen TANG Yang WANG Hubao A Zhen HE kai WU Lei WANG State Key Laboratory of Water Resources Engineering and Management Wuhan UniversityWuhan430072China Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education School of Materials Science and EngineeringTongji UniversityShanghai200092China College of Materials Science and Engineering Xi’an University of Architecture and TechnologyXi’an710055China

The time-dependent viscoelastic response of cement-based materials to applied deformation is far from fully understood at the atomic *** silicate hydrate(C-S-H),the main hydration product of Portland cement,is responsible for the viscoelastic mechanism of cement-based *** this study,a molecular model of C-S-H was developed to explain the stress relaxation characteristics of C-S-H at different initial deformation states,Ca/Si ratios,temperatures,and water contents,which cannot be accessed *** stress relaxation of C-S-H occurs regardless of whether it is subjected to initial shear,tensile,or compressive deformation,and shows a heterogeneous *** plays a crucial role in the stress relaxation process.A large Ca/Si ratio and high temperature reduce the cohesion between the calcium-silicate layer and the interlayer region,and the viscosity of the interlayer region,thereby accelerating the stress relaxation of *** effect of the hydrogen bond network and the morphology of C-S-H on the evolution of the stress relaxation characteristics of C-S-H at different water contents was elucidated by nonaffine mean squared *** results shed light on the stress relaxation characteristics of C-S-H from a microscopic perspective,bridging the gap between the microscopic phenomena and the underlying atomic-level mechanisms.

关键词： Calcium silicate hydrate(C-S-H) Stress relaxation Ca/Si ratio Temperature Water content Atomic simulation

ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

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World Journal of Gastroenterology 2024年第12期30卷 1764-1776页

作者： Tao Zhang Shuai Zhu geng-wen Huang Department of General Surgery Xiangya HospitalCentral South UniversityChangsha 410005HunanChina. National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410005HunanChina.

BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological *** autophagy is a critical hallmark of acute pancreatitis(AP).AIM To explore the role of the m6A modification of ZKSCAN3 in the regulation of autophagy in *** The AP mouse cell model was established by cerulein-treated mouse pancreatic acinar cells(MPC-83),and the results were confirmed by the levels of amylase and inflammatory *** activity was evaluated by specific identification of the autophagy-related microstructure and the expression of autophagy-related ***3 and ALKBH5 were knocked down to study the function in AP.A m6A RNA binding protein immunoprecipitation assay was used to study how the m6A modification of ZKSCAN3 mRNA is regulated by *** The increased expression of amylase and inflammatory factors in the supernatant and the accumulation of autophagic vacuoles verified that the AP mouse cell model was *** downregulation of LAMP2 and upregulation of LC3-II/I and SQSTM1 demonstrated that autophagy was impaired in *** expression of ZKSCAN3 was upregulated in *** of ZKSCAN3 increased the expression of LAMP2 and decreased the expression of the inflammatory factors,LC3-II/I and ***,ALKBH5 was upregulated in *** of ALKBH5 downregulated ZKSCAN3 expression and restored decreased autophagic flux in ***,the bioinformatic analysis revealed 23 potential m6A modification sites on ZKSCAN3 *** m6A modification of ZKSCAN3 mRNA was significantly decreased in *** of ALKBH5 increased the modification of ZKSCAN3 mRNA,which confirmed that ALKBH5 upregulated ZKSCAN3 expression in a m6A-dependent *** ALKBH5 inhibits autophagic flux through m6A demethylation of ZKSCAN3 mRNA in AP,thereby aggravating the severity of the disease.

关键词： Acute pancreatitis Autophagy ZKSCAN3 N6-methyladenosine ALKBH5

Molecular recognition of cyclophanes in water

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Chinese Chemical Letters 2024年第5期35卷 32-44页

作者： Fang-Yuan Chen wen-Chao geng Kang Cai Dong-Sheng Guo College of Chemistry State Key Laboratory of Elemento-Organic ChemistryKey Laboratory of Functional Polymer Materials(Ministry of Education)Frontiers Science Center for New Organic MatterCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China College of Chemistry Nankai UniversityTianjin 300071China

Molecular recognition in water,the biological solvent,always receives significant research focus in supramolecular *** mechanisms of molecular recognition in water is key to comprehending biological processes at the molecular *** the past five decades,supramolecular chemists have developed a vast array of synthetic receptors with highly diverse structures and recognition *** them,cyclophanes represent an important family of macrocyclic receptors that have been extensively *** aromatic moieties in cyclophanes not only facilitate chemical modifications to impart water solubility but also enable forming hydrophobic cavities for guest inclusion in aqueous *** by Koga et al.,who reported the first inclusion complex of cyclophanes in water and solid state,numerous water-soluble cyclophanes,including derivatives of blue box,calixarenes,resorcinarenes,pillararenes,octopusarenes,biphenarenes,coronarenes,and naphthotubes,etc.,have been synthesized and subjected to investigation of the recognition capabilities in aqueous *** review provides an overview of cyclophane receptors designed to bind organic guests in *** categorize them into two classes based on the modifications made to their hydrophobic cavities:those with“exo-functionalized hydrophobic cavities”and those with“endo-functionalized hydrophobic cavities”.We introduce their distinctive features and discuss strategies to enhance recognition affinity and *** review aims to inspire the development of novel synthetic receptors with intriguing properties and foster practical applications of cyclophanes.

关键词： Biomimetic receptor Cyclophane Molecular recognition Hydrophobic effect High-affinity Macrocycles

Sinomenine increases osteogenesis in mice with ovariectomyinduced bone loss by modulating autophagy

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World Journal of Stem Cells 2024年第5期16卷 486-498页

作者： Hai-Xiang Xiao Lei Yu Yu Xia kai Chen wen-Ming Li Gao-Ran Ge Wei Zhang Qing Zhang Hong-Tao Zhang De-Chun geng Department of Orthopedics The Fourth Affiliated Hospital of Soochow UniversitySuzhou Dushu Lake HospitalMedical Centre of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Orthopedics Jingjiang People’s Hospital Affiliated to Yangzhou UniversityJingjiang 214500Jiangsu ProvinceChina Department of Orthopedics The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Orthopedics Hai’an People’s HospitalHai’an 226600Jiangsu ProvinceChina Department of Orthopedics The Affiliated Huai’an Hospital of Xuzhou Medical UniversityHuai’an Second People’s HospitalXuzhou 223002Jiangsu ProvinceChina Department of Orthopaedics The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND A decreased autophagic capacity of bone marrow mesenchymal stromal cells(BMSCs)has been suggested to be an important cause of decreased osteogenic differentiation.A pharmacological increase in autophagy of BMSCs is a potential therapeutic option to increase osteoblast viability and ameliorate *** To explore the effects of sinomenine(SIN)on the osteogenic differentiation of BMSCs and the underlying *** For in vitro experiments,BMSCs were extracted from sham-treated mice and ovariectomized mice,and the levels of autophagy markers and osteogenic differentiation were examined after treatment with the appropriate concen-trations of SIN and the autophagy inhibitor *** vivo,the therapeutic effect of SIN was verified by establishing an ovariectomy-induced mouse model and by morphological and histological assays of the mouse *** SIN reduced the levels of AKT and mammalian target of the rapamycin(mTOR)phosphorylation in the phosphatidylinositol 3-kinase(PI3K)/AKT/mTOR signaling pathway,inhibited mTOR activity,and increased autophagy ability of BMSCs,thereby promoting the osteogenic differentiation of BMSCs and effectively alleviating bone loss in ovariectomized mice in *** The Chinese medicine SIN has potential for the treatment of various types of osteoporosis,bone homeostasis disorders,and autophagy-related diseases.

关键词： Sinomenine Osteogenesis Autophagy Ovariectomy Osteoporosis

Metal ion metabolism and osteoporosis:possible implications for pharmaceutical biotechnology and tissue engineering

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Science China(Life Sciences) 2024年第8期67卷 1763-1765页

作者： Huaqiang Tao Qiufei Wang kai Chen Pengfei Zhu Ye Gu Dechun geng Department of Orthopedics The First Affiliated Hospital of Soochow UniversitySuzhou 215000China Department of Orthopedics Changshu Hospital Affiliated to Soochow UniversityFirst People’s Hospital of Changshu CityChangshu 215506China Department of Orthopedics Hai’an People’s HospitalHai’an 226600China

Osteoporosis(OP)is a systemic metabolic bone disease characterized by low bone mass,damage to bone microstructure and increased bone *** imbalance of functional coupling between osteoblasts and osteoclasts contributes to the pathological progression of the disease(Rachner et al.,2011).The pathogenesis of OP is precisely controlled by the complex regulatory network of the bone *** elements,although present in small amounts in the body,are essential for their influence on the physiological activities of *** ions act as effective catalysts and have applications in all stages of cell biochemical evolution(Qian et al.,2021).Bone tissue contains various metal elements,and metal ions play indispensable roles in bone metabolism at the molecular and cellular levels(Luo et al.,2023).However,it is important to note that these metal ions can also contribute to the progression of OP by influencing multiple cellular pathways either directly or indirectly.

关键词： metabolism biotechnology indispensable

Highly Stretchable and Tunable Thermo-fluorochromic Polymer Elastomer through Eu3+ Dynamic Coordination Cross-linking

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Chinese Journal of Polymer Science 2024年第10期42卷 1449-1458页

作者： Qiu-Li Tan kai Gou Jing-Zhi Tang Ming-Hui Wei Chong Wang Yi-Jing Nie geng-Sheng weng School of Materials Science and Chemical Engineering State Key Laboratory Base of Novel Functional Materials and Preparation Science Ningbo University Institute of Polymer Materials School of Materials Science and Engineering Jiangsu University

Thermochromic polymers with tunable thermochromism, high stretchability and mechanical strength are of interests for optical information storage and encryption. In this work, we demonstrate a new design of thermo-fluorochromic carboxylated nitrile butadiene rubber(XNBR) elastomer cross-linked by Eu3+-COOH dynamic coordination with deprotonated imidazole (DPIm) as the ancillary ligand. The presence of DPIm not only improves the mechanical strength and stretchability, but also dramatically intensifies the fluorescence emission and lifetime of the Eu-containing elastomer. The elastomer behaves reversible thermo-fluorochromism with easily tunable transition temperature and emission intensity by the simple change of the deprotonation degree of imidazole. The facile tunable thermo-fluorochromism, exceptional mechanical strength, and high stretchability (up to about 5000%) enable the Eu-containing XNBR elastomer with potential applications in soft electronics where optical information storage and encryption are required.

关键词： Dynamic coordination Elastomer Europium Highly stretchable Thermochromism

Effects of intranasal oxytocin on social transfer of pain in rodents

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Effects of intranasal oxytocin on social transfer of pain in...