检索结果-南通市图书馆

A novel norsesquiterpene alkaloid from the mushroom-forming fungus Flammulina velutipes

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2013年第1期24卷 57-58页

作者： Zhen-Yu Xu Zhi-Ang Wu kai-shun bi School of Business Administration Shenyang Pharmaceutical University Center for Drug Evaluation SFDA

A new norsesquiterpe alkaloid （1） was isolated from the solid culture of mushroom-forming fungus Flammulina velutipes fermented on rice. The structure of I was elucidated by spectroscopic methods. The absolute confi... 详细信息

关键词： MushroomNorsesquiterpeneFlammulina velutipesCytotoxicity

QUALITY ASSESSMENT OF GINSENG BY CHEMICAL FUZZY PATTERN RECOGNITION

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1991年第5期2卷 391-392页

作者： kai shun bi Xu LUO Xi WANG Shenyang College of Pharmacy Shenyang 110015

A new method of assessing ginseng and screening its bioactive constituents by fuzzy pattern recognition has been proposed.

关键词： QUALITY ASSESSMENT OF GINSENG BY CHEMICAL FUZZY PATTERN RECOGNITION HPLC

Authentication and distinction of Shenmai injection with HPLC fingerprint analysis assisted by pattern recognition techniques

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2012年第5期2卷 327-333页

作者： Xue-Feng Lu kai-shun bi Xu Zhao Xiao-Hui Chen School of Pharmacy Shenyang Pharmaceutical UniversityWenhua Road 103Shenhe DistrictShenyang 110016China

In this paper, the feasibility and advantages of employing high performance liquid chromatographic （HPLC） fingerprints combined with pattern recognition techniques for quality control of Shenmai injection were investigated and demonstrated. The Similarity Evaluation System was employed to evaluate the similarities of samples of Shenmai injection, and the HPLC generated chromatographic data were analyzed using hierarchical clustering analysis （HCA） and soft independent modeling of class analogy （SIMCA）. Consistent results were obtained to show that the authentic samples and the blended samples were successfully classified by SIMCA, which could be applied to accurate discrimination and quality control of Shenmai injection. Furthermore, samples could also be grouped in accordance with manufacturers. Our results revealed that the developed method has potential perspective for the original discrimination and quality control of Shenmai injection.

关键词： Shenmai injection High performance liquidchromatography Fingerprint Pattern recognition

Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2011年第3期1卷 191-196页

作者： Ruo-Wen Zhang Wen-Tao Liu Lu-Lu Geng Xiao-Hui Chen kai-shun bi School of Pharmacy Shenyang Pharmaceutical University Shenyang 110016 China School of Pharmacy Medical Biology Centre Queen's University Belfast UK

We described the first results of a quantitative ultra performance liquid chromatographytandem mass spectrometry method for a novel antimicrobial peptide （phylloseptin, PSN-1）. Chromatographic separation was accomplished on a Waters bridged ethyl hybrid （BEH） C18 （50mm× 2.1 mm, 1.7 μm） column with acetonitrile-water （25：75, v/v） as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 （PSN-1） and m/z 340.7/165 （Thymopentin, IS）. Protein precipitation was investigated and the recovery was satisfactory （above 82%）. The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 lag/mL with r〉0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration.

关键词： Antimicrobial peptide Phylloseptin Ultra performanceliquid chromatographytandem massspectrometry Pharmacokinetie

Simultaneous determination of four active components in Alisma orientale (Sam.) Juz. by HPLC–DAD using a single reference standard

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2015年第2期5卷 85-92页

作者： Yao-Wen Zhang Qing Li Chun-Xiao Lv Xiu-Jia Liu Xiao-Hui Chen kai-shun bi School of Pharmacy Shenyang Pharmaceutical University Liaoning Institute for Drug Control National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control Shenyang Pharmaceutical University

A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector（HPLC–DAD） was developed to evaluate the quality of Alisma orientale（Sam.） *** a simultaneous determination of four major active triterpenes using a single standard to determine the multi-components（SSDMCs）. Alisol B 23-acetate was selected as the reference compound for calculating the relative response factors. All calibration curves showed good linearity（R240.9998） within test ranges. RSDs for intra- and inter-day of four analytes were less than 3.6% and 2.3%; the overall recovery was 92.1–110.2%（SSDMC）. The proposed method was successfully applied to quantify the four components in 20 samples from different localities in China. Moreover, significant variations were demonstrated in the content of these compounds. In addition, hierarchical clustering analysis（HCA） and principal components analysis（PCA） were performed to differentiate and classify the samples based on the contents of Alisol C 23-acetate, Alisol A, Alisol A 24-acetate and Alisol B 23-acetate. This simple,rapid, low-cost and reliable HPLC–DAD method using SSDMC is suitable for routine quantitative analysis and quality control of A. orientale（Sam.） Juz.

关键词： SSDMC Alisma orientale(Sam.) Juz Quality control HCA PCA

Development of the fingerprints for the quality evaluation of Viscum coloratum by high performance liquid chromatography

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2011年第2期1卷 113-118页

作者： Yun-Li Zhao Rong-Hua Fan Hong-Xia Yuan Miao Yu kai-shun bi Zhi-Guo Yu Shenyang Pharmaceutical University Shenyang 110016 China Pharmaland Technology Development Co. Ltd Tianjin Economic Technological Development Area Tianjin 300457 China Shanxi College of Traditional Chinese Medicine Taiyuan 030024 China

A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.

关键词： Viscum coloratum quality high-performance liquid chromatography fingerprint

Quantitative Evaluation of the Compatibility Effects of Aidi Injection on the Treatment of Hepatocellular Carcinoma Using Targeted Metabolomics:A New Strategy on the Mechanism Study of an Anticancer Compound in Traditional Chinese Medicine

在线全文

学校读者我要写书评

暂无评论

World Journal of Traditional Chinese Medicine 2021年第1期7卷 111-119页

作者： Ran Liu Lin-Lin Zhu Chun-Yu Yu Ya-Ping Shuai Ling-Ling Sun kai-shun bi Qing Li School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China Liaoning Institute of Drug Inspection and Testing Shenyang 110030China

Objective:Compound traditional Chinese medicine(CTCM)with the application of compatibility from multiple active ingredients with multiple-specific targets can achieve a synergistic effect on cancer *** study is aimed to observe the compatibility effects of Aidi injection on the treatment of hepatocellular carcinoma and to explore the mechanism of ***:Aidi injection is a clinical compound prescription containing Mylabris,Ginseng,Astragalus,and Acanthopanax,which can inhibit tumor growth and induce *** this study,the anticancer activity of Aidi injection,as well as its disassembled and combined compositions,had been evaluated by varying levels of polyamine biomarkers on human hepatoma Hep-G2 cells detected using ultrahigh-performance liquid chromatography-tandem mass ***:According to the different variations in polyamine levels,it was revealed that Mylabris and Ginseng had an antitumor effect,while Astragalus acted as an assistant and Acanthopanax had weak anticancer *** increased level of polyamines in Hep-G2 cells had been found in HL-7702 *** combining Mylabris and Ginseng,polyamine levels went close to the normal level,which was even more marked when Astragalus was *** injection acted like the combination of Mylabris,Ginseng,and ***:This study established a quantitative evaluation of the compatibility effects of Aidi injection based on polyamine biomarkers and evaluated the consistency of its anticancer effect,providing a manner to research the efficacy evaluation of ***,the correlation between polyamine metabolism and anticancer activity can be used in anticancer drug screening.

关键词： Compatibility effects evaluation compound traditional Chinese medicine hepatocellular carcinoma polyamine biomarker

LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of suspensions of traditional Chinese medicine Erzhi Wan and Fructus Ligustri lucidi

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2011年第1期1卷 8-12页

作者： Ze-Yun Li Qing Li Jiang Lu Jun-Hong Ling Xi-Hua Yu Xiao-Hui Chen kai-shun bi School of Pharmacy Shenyang Pharmaceutical University Shenyang 110016 China.

A simple,rapid and sensitive liquid chromatography-mass spectrometry（LC-MS）method was developed for the determination of salidroside in rat plasma and study of its pharmacokinetics after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi into Wistar *** sample of 200 μL was extracted with acetic ether-isopropanol（2∶1）and the extraction was performed on a Kromasil C18 column（150 mm×4.6 mm,5 μm）with the mobile phase of methanol-water（41∶59,v/v）within a run time of 6.0 *** analyte was monitored with positive electrospray ionization（ESI）by selected ion monitoring（SIM）*** target ions were m/z 323.05 for salidroside and m/z 411.05 for internal standard（IS）geniposide.A good linear relationship was obtained over the range of 5.0-500.0 ng/mL and the lower limit of quantification was 5.0 ng/*** validated method was successfully applied to the pharmacokinetic study of salidroside in rat plasma after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi.

关键词： liquid chromatography-mass spectrometry(LC-MS) pharmacokinetics salidroside Erzhi Wan Fructus Ligustri lucidi

Study on changes of polyamine levels in mice with the development of U14 cervical cancer

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2013年第1期3卷 20-27页

作者： Qian Wang Yi-Xiang Wang Ran Liu Yan Zhou Ying Jia Xiang-Lin Wang Yu Hu kai-shun bi Qing Li School of Pharmacy Shenyang Pharmaceutical University School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenzhen Rainbow Light Marketing Plans Co. Ltd.

This study was performed to investigate the possible involvement of polyamincs in the development of cervical cancer. The objective of the present study was therefore to lind the specilic polyamine indicators, which could be used as useful markers for the early determination of cervical cancer. A simple method for the simultaneous determination of plasma concentrations ol live polyamines in normal and UI4 model mice was developed by using HPLC-MS. The samples were derivatized by benzoyl chloride. The derived polyamines were separated on a C18 colunm by a gradient elution with methanol water, and then detected with HPLC-MS. The results showed tha! all polyamine levels in the U 14 model mice were higher than those in normal ones. Thc cadaverine. putrescine and 1,3-diaminopropane levels were significantly higher in U 14 model mice plasma than those in normal mice plasma, especially the putrescine and 1, 3-diaminopropane （P〈0.01）. The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U 14 mice model urine than those in normal mice urine, especially the cadaverine and 1, 3-diaminopropane （P〈0.01）. Putrescine, cadaverine and I, 3-diaminopropane might be the indicators of the cervical cancer.

关键词： Polyamines Cervical cancer Plasma Urine Mice HPLC-MS