Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry

作     者：Ruo-Wen Zhang Wen-Tao Liu Lu-Lu Geng Xiao-Hui Chen Kai-Shun Bi 

作者机构：School of Pharmacy Shenyang Pharmaceutical University Shenyang 110016 China School of Pharmacy Medical Biology Centre Queen's University Belfast UK 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2011年第1卷第3期

页      面：191-196页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 0817[工学-化学工程与技术] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 0702[理学-物理学] 

基　　金：Chao Ma for analysis assistance 

主　　题：Antimicrobial peptide Phylloseptin Ultra performanceliquid chromatographytandem massspectrometry Pharmacokinetie 

摘      要：We described the first results of a quantitative ultra performance liquid chromatographytandem mass spectrometry method for a novel antimicrobial peptide （phylloseptin, PSN-1）. Chromatographic separation was accomplished on a Waters bridged ethyl hybrid （BEH） C18 （50mm× 2.1 mm, 1.7 μm） column with acetonitrile-water （25：75, v/v） as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 （PSN-1） and m/z 340.7/165 （Thymopentin, IS）. Protein precipitation was investigated and the recovery was satisfactory （above 82%）. The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 lag/mL with r〉0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration.