检索结果-南通市图书馆

Sinomenine increases osteogenesis in mice with ovariectomyinduced bone loss by modulating autophagy

World Journal of Stem Cells 2024年第5期16卷 486-498页

作者： Hai-xiang xiao Lei Yu Yu xia kai Chen Wen-Ming Li Gao-Ran Ge Wei Zhang Qing Zhang Hong-Tao Zhang De-Chun Geng Department of Orthopedics The Fourth Affiliated Hospital of Soochow UniversitySuzhou Dushu Lake HospitalMedical Centre of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Orthopedics Jingjiang People’s Hospital Affiliated to Yangzhou UniversityJingjiang 214500Jiangsu ProvinceChina Department of Orthopedics The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Orthopedics Hai’an People’s HospitalHai’an 226600Jiangsu ProvinceChina Department of Orthopedics The Affiliated Huai’an Hospital of Xuzhou Medical UniversityHuai’an Second People’s HospitalXuzhou 223002Jiangsu ProvinceChina Department of Orthopaedics The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND A decreased autophagic capacity of bone marrow mesenchymal stromal cells(BMSCs)has been suggested to be an important cause of decreased osteogenic differentiation.A pharmacological increase in autophagy of BMSCs is a potential therapeutic option to increase osteoblast viability and ameliorate osteoporosis.AIM To explore the effects of sinomenine(SIN)on the osteogenic differentiation of BMSCs and the underlying mechanisms.METHODS For in vitro experiments,BMSCs were extracted from sham-treated mice and ovariectomized mice,and the levels of autophagy markers and osteogenic differentiation were examined after treatment with the appropriate concen-trations of SIN and the autophagy inhibitor 3-methyladenine.In vivo,the therapeutic effect of SIN was verified by establishing an ovariectomy-induced mouse model and by morphological and histological assays of the mouse femur.RESULTS SIN reduced the levels of AKT and mammalian target of the rapamycin(mTOR)phosphorylation in the phosphatidylinositol 3-kinase(PI3K)/AKT/mTOR signaling pathway,inhibited mTOR activity,and increased autophagy ability of BMSCs,thereby promoting the osteogenic differentiation of BMSCs and effectively alleviating bone loss in ovariectomized mice in vivo.CONCLUSION The Chinese medicine SIN has potential for the treatment of various types of osteoporosis,bone homeostasis disorders,and autophagy-related diseases.

关键词： Sinomenine Osteogenesis Autophagy Ovariectomy Osteoporosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

SRY-Box transcription factor 9 triggers YAP nuclear entry via direct interaction in tumors

引用

Signal Transduction and Targeted Therapy 2024年第5期9卷 2285-2297页

作者： Hui Qian Chen-Hong Ding Fang Liu Shi-Jie Chen Chen-kai Huang Meng-Chao xiao xia-Lu Hong Ming-Chen Wang Fang-Zhi Yan kai Ding Ya-Lu Cui Bai-Nan Zheng Jin Ding Cheng Luo Xin Zhang Wei-Fen Xie Department of Gastroenterology Changzheng HospitalNaval Medical UniversityShanghaiChina Department of Gastroenterology Shanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina Drug Discovery and Design Center CAS Key Laboratory of Receptor ResearchState Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina Department of Gastroenterology First Affiliated Hospital of Nanchang UniversityNanchangChina Clinical Cancer Institute Center for Translational MedicineNaval Medical UniversityShanghaiChina

The translocation of YAP from the cytoplasm to the nucleus is critical for its activation and plays a key role in tumor progression.However,the precise molecular mechanisms governing the nuclear import of YAP are not fully understood.In this study,we have uncovered a crucial role of SOX9 in the activation of YAP.SOX9 promotes the nuclear translocation of YAP by direct interaction.Importantly,we have identified that the binding between Asp-125 of SOX9 and Arg-124 of YAP is essential for SOX9-YAP interaction and subsequent nuclear entry of YAP.Additionally,we have discovered a novel asymmetrical dimethylation of YAP at Arg-124(YAP-R124me2a)catalyzed by PRMT1.YAP-R124me2a enhances the interaction between YAP and SOX9 and is associated with poor prognosis in multiple cancers.Furthermore,we disrupted the interaction between SOX9 and YAP using a competitive peptide,S-A1,which mimics anα-helix of SOX9 containing Asp-125.S-A1 significantly inhibits YAP nuclear translocation and effectively suppresses tumor growth.This study provides the first evidence of SOX9 as a pivotal regulator driving YAP nuclear translocation and presents a potential therapeutic strategy for YAP-driven human cancers by targeting SOX9-YAP interaction.

关键词： interaction. interaction precise

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epigenetic regulation by long noncoding RNAs in osteo-/adipogenic differentiation of mesenchymal stromal cells and degenerative bone diseases

引用

World Journal of Stem Cells 2022年第1期14卷 92-103页

作者： kai xia Li-Yuan Yu Xin-Qi Huang Zhi-He Zhao Jun Liu State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Orthodontics West China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina

Bone is a complex tissue that undergoes constant remodeling to maintain homeostasis,which requires coordinated multilineage differentiation and proper proliferation of mesenchymal stromal cells(MSCs).Mounting evidence indicates that a disturbance of bone homeostasis can trigger degenerative bone diseases,including osteoporosis and osteoarthritis.In addition to conventional genetic modifications,epigenetic modifications(i.e.,DNA methylation,histone modifications,and the expression of noncoding RNAs)are considered to be contributing factors that affect bone homeostasis.Long noncoding RNAs(lncRNAs)were previously regarded as‘transcriptional noise’with no biological functions.However,substantial evidence suggests that lncRNAs have roles in the epigenetic regulation of biological processes in MSCs and related diseases.In this review,we summarized the interactions between lncRNAs and epigenetic modifiers associated with osteo-/adipogenic differentiation of MSCs and the pathogenesis of degenerative bone diseases and highlighted promising lncRNA-based diagnostic and therapeutic targets for bone diseases.

关键词： Long noncoding RNA Epigenetics DNA methylation Histones Cell differentiation Bone diseases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

引用

International Journal of Oral Science 2022年第3期14卷 360-370页

作者： Liyuan Yu kai xia Jing Zhou Zhiai Hu Xing Yin Chenchen Zhou Shujuan Zou Jun Liu State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Orthodontics West China Hospital of StomatologySichuan UniversityChengduChina Department of Stomatology Kunming Yan’an hospitalKunmingChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina

Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.

关键词： regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis miR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overlooked uneven progress across sustainable development goals at the global scale: Challenges and opportunities

引用

The Innovation 2024年第2期5卷 42-50页

作者： Yali Liu Jianqing Du Yanfen Wang xiaoyong Cui Jichang Dong Pan Gu Yanbin Hao kai Xue Hongbo Duan Anquan xia Yi Hu Zhi Dong Bingfang Wu Jüurgen P.Kropp Bojie Fu School of Grassland Science Beijing Forestry UniversityBeijing 100083China Beijing Yanshan Earth Critical Zone National Research Station University of Chinese Academy of SciencesBeijing 101408China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Tibetan Plateau Earth System Environment and ResourcesChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China School of Economics and Management University of Chinese Academy of SciencesBeijing 100190China School of Humanities University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001China School of Innovation and Entrepreneurship University of Chinese Academy of SciencesBeijing 100190China State Key Laboratory of Remote Sensing Science Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100101China Potsdam Institute for Climate Impact Research 14412 PotsdamGermany Institute for Environmental Science and Geography University of Potsdam14412 PotsdamGermany State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

Differences in progress across sustainable development goals(SDGs)are widespread globally;meanwhile,the rising call for prioritizing specific SDGs may exacerbate such gaps.Nevertheless,how these progress differences would influence global sustainable development has been long neglected.Here,we present the first quantitative assessment of SDGs’progress differences globally by adopting the SDGs progress evenness index.Our results highlight that the uneven progress across SDGs has been a hindrance to sustainable development because(1)it is strongly associated with many public health risks(e.g.,air pollution),social inequalities(e.g.,gender inequality,modern slavery,wealth gap),and a reduction in life expectancy;(2)it is also associated with deforestation and habitat loss in terrestrial and marine ecosystems,increasing the challenges related to biodiversity conservation;(3)most countries with low average SDGs performance show lower progress evenness,which further hinders their fulfillment of SDGs;and(4)many countries with high average SDGs performance also showcase stagnation or even retrogression in progress evenness,which is partly ascribed to the antagonism between climate actions and other goals.These findings highlight that while setting SDGs priorities may be more realistic under the constraints of multiple global stressors,caution must be exercised to avoid new problems from intensifying uneven progress across goals.Moreover,our study reveals that the urgent needs regarding SDGs of different regions seem complementary,emphasizing that regional collaborations(e.g.,demand-oriented carbon trading between SDGs poorly performed and well-performed countries)may promote sustainable development achievements at the global scale.

关键词： globally priori inequality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of the Mathieu combined tunnel technique for repairing glans dehiscence after failed hypospadias repair

引用

Asian Journal of Andrology 2022年第3期24卷 311-316页

作者： Qi-Gen Xie kai xia xiang-Ping Li Peng Luo Zuo-Qing Li Cheng Su Chun-Hua Deng Department of Pediatric Surgery First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510080China Department of Andrology First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510080China

Repairing glans dehiscence after failed hypospadias repair is challenging for pediatric surgeons.Here,we introduced and evaluated a newly modified Mathieu technique,Mathieu combined tunnel(MCT),which involves multiple custom-designed flaps for the shortage of flap source material after repeated operations;we also constructed a tunnel to avoid the glans incision that may carry new risks of dehiscence.This retrospective study included 26 patients who were consecutively admitted to the First Affiliated Hospital of Sun Yat-Sen University(Guangzhou,China)for glans dehiscence repair after failed hypospadias repair from October 2014 to October 2020;sixteen patients underwent surgery using the MCT(MCT group)and ten patients underwent surgery using the tubularized incised plate(TIP)technique(TIP group).The operative time,blood loss,postoperative complications,normal urethral meatus rate,success rate,and Hypospadias Objective Penile Evaluation(HOPE)score were compared between the two groups.The MCT group achieved an overall satisfactory penile appearance and voiding function,with a higher rate of normal urethral meatus(15/16,93.8%)and a lower rate of glans dehiscence(1/16,6.2%),compared with the TIP group(70.0%and 30.0%,respectively).However,these differences were not statistically significant,possibly because of the limited number of patients(all P>0.05).Mean postoperative HOPE scores were similar in the MCT group(mean±standard deviation:8.83±0.89)and TIP group(8.94±0.57)(P>0.05).No significant differences were found between the two groups in terms of blood loss and success rate,nor in the rates of various complications(e.g.,fistula,urethral stricture,and glans dehiscence).In conclusion,the MCT technique appears to be feasible and reliable for repairing glans dehiscence after failed hypospadias repair.

关键词： failed hypospadias glans dehiscence Mathieu tubularized incised plate(TIP) tunnel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Timing of individualized surgical intervention in Crohn’s disease

引用

World Journal of Gastrointestinal Surgery 2022年第12期14卷 1320-1328页

作者： kai xia Ren-Yuan Gao xiao-Cai Wu Lu Yin Chun-Qiu Chen Diagnostic and Treatment Center for Refractory Diseases of Abdomen Surgery Shanghai Tenth People’s HospitalTongji University School of MedicineShanghai 200072China

Crohn’s disease(CD)is a chronic inflammatory disorder of the gastrointestinal tract with an increasing incidence worldwide.Comprehensive therapy for CD focuses on symptom control and healing the intestinal mucosa to improve the quality of life and prevent complications.Surgical intervention plays a vital role in comprehensive therapy.However,deciding the optimal timing for surgical intervention has long been a focus of controversy.This review provides insights into the timing of surgery for CD and guides clinicians in daily treatment.

关键词： Crohn’s disease Surgical intervention Timing of surgery Individualization Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Breaking the linear correlations for enhanced electrochemical nitrogen reduction by carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6)

引用

Journal of Energy Chemistry 2022年第8期31卷 182-187,I0006页

作者： Lehui Ma Fanfan Xu Linlin Zhang Zhongfen Nie kai xia Mingxia Guo Mingzhu Li Xin Ding College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071ShandongChina

Electrochemical nitrogen reduction(NRR)is deemed as a consummate answer for the traditional Haber–Bosch technology.Breaking the linear correlations between adsorption and transition-state energies of intermediates is vital to improve the kinetics of ammonia synthesis and obtain a less energy-intensive process.Herein,carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6) was prepared and applied as an electrocatalyst for high-efficiency NRR.A dramatic faradaic efficiency(FE)of 36.93%and an NH_(3) production rate of 13.1μg h^(-1) mg_(cat)^(-1) were obtained at-0.3 V versus RHE,superior to nearly all Fe-based catalysts.Experiments and DFT calculations revealed that the superior performance was ascribed to the synergistic effect of mixed-valence iron pair,which braked the linear correlations to improve the kinetics of ammonia from collaborative hydrogenation and*NH_(3) separation.This work proves the feasibility of mixedvalence catalysts for nitrogen reduction and thus opening a new avenue towards artificial nitrogenfixation catalysts.

关键词： N2 reduction reaction(NRR) Mixed-valence Linear correlations Nitrogen fixation N_(2)activation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multi-surrogate framework with an adaptive selection mechanism for production optimization

引用

Petroleum Science 2024年第1期21卷 366-383页

作者： Jia-Lin Wang Li-Ming Zhang kai Zhang Jian Wang Jian-Ping Zhou Wen-Feng Peng Fa-Liang Yin Chao Zhong xia Yan Pi-Yang Liu Hua-Qing Zhang Yong-Fei Yang Hai Sun School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Qingdao University of Technology Qingdao266580ShandongChina College of Science China University of PetroleumQingdao266580ShandongChina Zepu Oil and Gas Development Department PetroChina Tarim Oilfield CompanyKorla841000XinjiangChina Hainan Branch of CNOOC Ltd Haikou70311HainanChina

Data-driven surrogate models that assist with efficient evolutionary algorithms to find the optimal development scheme have been widely used to solve reservoir production optimization problems.However,existing research suggests that the effectiveness of a surrogate model can vary depending on the complexity of the design problem.A surrogate model that has demonstrated success in one scenario may not perform as well in others.In the absence of prior knowledge,finding a promising surrogate model that performs well for an unknown reservoir is challenging.Moreover,the optimization process often relies on a single evolutionary algorithm,which can yield varying results across different cases.To address these limitations,this paper introduces a novel approach called the multi-surrogate framework with an adaptive selection mechanism(MSFASM)to tackle production optimization problems.MSFASM consists of two stages.In the first stage,a reduced-dimensional broad learning system(BLS)is used to adaptively select the evolutionary algorithm with the best performance during the current optimization period.In the second stage,the multi-objective algorithm,non-dominated sorting genetic algorithm II(NSGA-II),is used as an optimizer to find a set of Pareto solutions with good performance on multiple surrogate models.A novel optimal point criterion is utilized in this stage to select the Pareto solutions,thereby obtaining the desired development schemes without increasing the computational load of the numerical simulator.The two stages are combined using sequential transfer learning.From the two most important perspectives of an evolutionary algorithm and a surrogate model,the proposed method improves adaptability to optimization problems of various reservoir types.To verify the effectiveness of the proposed method,four 100-dimensional benchmark functions and two reservoir models are tested,and the results are compared with those obtained by six other surrogate-model-based methods.The results demonstrate that our approach can obtain th

关键词： Production optimization Multi-surrogate models Multi-evolutionary algorithms Dimension reduction Broad learning system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-efficiency gene editing in Anopheles sinensis using ReMOT control

引用

Insect Science 2024年第1期31卷 307-312页

作者： xiao-lin Yang xia Ling Quan Sun Pin-pin Qiu kai xiang Jun-feng Hong Shu-lin He Jie Chen Xin Ding Hai Hu Zheng-bo He Cao Zhou Bin Chen Liang Qiao Chongqing Key Laboratory of Vector Insects Institute of Entomology and Molecular BiologyCollege of Life SciencesChongqing Normal UniversityChongqingChina Xunjian Life Science&Technology Co.Ltd. ChongqingChina State Key Laboratory of Resource Insects Southwest UniversityChongqingChina

Dear editor,Functional genomics studies of mosquito vectors are crucial for understanding their physiological behavior,pathogen transmission,and evolution,and for providing essential molecular targets for genetic control(Severson&Behura,201l;Alphey,2014;Ruzzante et al.,2019;Hong et al.,2022).The main methods involve clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene editing via embryo microinjection(Criscione et al.,2015;Kistler et al.,2015).

关键词： editor editing injection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：