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The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa： differences from the zona pellucida

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Asian Journal of Andrology 2011年第1期13卷 159-165页

作者： lulio C Chaivez Gerardo A de Blas Josd l de la Vega-Beltran Takuya Nishigaki Mayel Chirinos Maria Elena Gonzaez-Gonzalez Fernando larrea Alejandra Soiis Alberto Darszon Claudia l Trevino Department of Developmental Genetics and Molecular Physiology Biotechnology Institute UNAM Cuernavaca Mexico Department of Reproduction Biology National Institute of Medical Sciences and Nutrition ＇Salvador Zubiran＇ Mexico City Mexico

The acrosome reaction （AR）, an absolute requirement for spermatozoa and egg fusion, requires the influx of Ca2＋ into the spermatozoa through voltage-dependent Ca2＋ channels and store-operated channels. Maitotoxin （MTx）, a Ca2＋-mobilizing agent, has been shown to be a potent inducer of the mouse sperm AR, with a pharmacology similar to that of the zona pellucida （ZP）, possibly suggesting a common pathway for both inducers. Using recombinant human ZP3 （rhZP3）, mouse ZP and two MTx channel blockers （U73122 and U73343）, we investigated and compared the MTx- and ZP-induced ARs in human and mouse spermatozoa. herein, we report that MTx induced AR and elevated intracellular Ca2＋（[Ca2＋]~） in human spermatozoa, both of which were blocked by U73122 and U73343. These two compounds also inhibited the MTx-induced AR in mouse spermatozoa. In disagreement with our previous proposal, the AR triggered by rhZP3 or mouse ZP was not blocked by U73343, indicating that in human and mouse spermatozoa, the AR induction by the physiological ligands or by MTx occurred through distinct pathways. U73122, but not U73343 （inactive analogue）, can block phospholipase C （PlC）. Another PlC inhibitor, edelfosine, also blocked the rhZP3- and ZP-induced ARs. These findings confirmed the participation of a PlC-dependent signalling pathway in human and mouse zona protein-induced AR. Notably, edelfosine also inhibited the MTx-induced mouse sperm AR but not that of the human, suggesting that toxin-induced AR is PlC-dependent in mice and PlC-independent in humans.

关键词： acrosome reaction calcium channels human sperm maitotoxin mouse sperm

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Calcium handling:a strategy to fight neurodegeneration in Alzheimer's disease

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Neural Regeneration Research 2023年第12期18卷 2685-2686页

作者： livia la Barbera Elena Spoleti Marcello D’Amelio Department of Sciences and Technologies for humans and Environment UniversitàCampus Bio-Medico di RomaRomeItaly Department of Experimental Neurosciences IRCCS Santa Lucia FoundationRomeItaly Department of Medicine and Surgery UniversitàCampus Bio-Medico di RomaRomeItaly

In the last few years,preclinical and clinical studies identified the ventral tegmental area(VTA)as one of the first brain regions to be affected in the prodromal phase of Alzheimer's disease(AD).The VTA is a deep midbrain nucleus rich in dopaminergic neurons that innervates and releases dopamine(DA)in several cortical and subcortical brain *** plays a crucial role in the modulation of both cognitive and noncognitive functions and failure of its release underlies both cognitive and neuropsychiatric symptoms in patients with AD.

关键词： Alzheimer clinical release

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POlYMERIZATION AND ChARACTERIZATION OF hIGh CONDUCTIVITY AND GOOD ADhENSION POlYPYRROlE FIlMS FOR ElECTROMAGNETIC INTERFERENCE ShIElDING

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Chinese Journal of Polymer Science 2010年第6期28卷 923-930页

作者： Yong-sheng Qiao la-zhen Shen Tao Dou Ming hu Institute of Special Chemicals Taiyuan University of Technology Taiyuan 030024 China School of Chemistry and Chemical Engineering Shanxi Datong University Datong 037009 China School of Electronic Information Engineering Tianjin University Tianjin 300072 China

Polypyrrole（PPy） shows a favorable application in the electromagnetic interference（EMI） shielding due to its good electrical conductivity and outstanding air *** PPy films with high conductivity and good adhesion were successfully polymerized on the surface of insulating epoxy resin substrates using chemical *** factors affecting the properties of PPy films,such as the surface morphology,adhesion between PPy film and substrate,electrical conductivity,EMI shielding effectiveness（SE）,were *** adhesion was improved significantly through a three-step surface pretreatment of epoxy resin substrates including removing impurities,roughening,and surface modification with silane coupling *** enhancement in the conductivity of PPy films of about one order of magnitude was achieved by adding dopant in FeCl_3 *** higher the conductivity,the better the shielding *** sodium p-toluenesulfonate doped PPy film as example,EMI SE was in the practically useful range of about 30 dB over a wide frequency range from 30 Mhz to 1500 *** PPy film samples were characterized by scanning electron microscopy （SEM）,infrared spectra（IR）,X-ray photoelectron spectroscopy（XPS） and the flange coaxial transmission *** fourpoint probe method was used to measure conductivity of PPy films.

关键词： Polypyrrole film EMI Conductivity Adhesion

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Enzymatically prepared alginate oligosaccharides improve broiler chicken growth performance by modulating the gut microbiota and growth hormone signals

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Journal of Animal Science and Biotechnology 2023年第5期14卷 2107-2127页

作者： A la Teng Zhu la Yuqing Feng Die hu Yimei Feng Xiaolu Jin Dan liu Yuming Guo Gong Cheng Yongfei hu State Key laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China State Key laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China

Background Alginate oligosaccharide(AOS)holds great potential as a novel feed supplement in farm ***,the effects of AOS on chicken health and the underlying mechanisms are not fully *** study aimed to optimize the enzymatic preparation of AOS by using bacterial alginate lyases expressed in yeast,investigate the effects of the prepared AOS on the growth performance and gut health of broiler chickens,and reveal the underlying *** Five alginate lyases from bacteria were cloned into Pichia pastoris GS115 and the alginate lyase PDE9 was expressed at relatively high yield,activity and stability in *** trials were carried out using 3201-day-old male Arbor Acres broilers(four groups;8 replicates/group×10 chicks/replicate)receiving either a basal diet or the same diet supplemented with 100,200 and 400 mg/kg PDE9-prepared AOS for 42 *** results showed that dietary supplementation of 200 mg/kg AOS displayed the highest activity in promoting the birds’ADG and ADFI(Pliorated the intestinal morphology,absorption function and barrier function,as indicated by the enhanced(Pl villus height,maltase activity,and the expression of PEPT,SGlT1,ZNT1,and *** also increased serum insulin-like growth factor-1,ghrelin(Ph hormone(Phe concentrations of acetate,isobutyrate,isovalerate,valerate,and total SCFAs in cecum of birds fed AOS were significantly higher than the control birds(Plysis indicated that AOS modulated the chicken gut microbiota structure,function,and microbial interactions and promoted the growth of SCFAs-producing bacteria,for example,Dorea sp.002160985;SCFAs,especially acetate,were found positively correlated with the chicken growth performance and growth-related hormone signals(Pher verified that AOS can be utilized by Dorea *** grow and to produce acetate in *** We demonstrated that the enzymatically

关键词： Acetate Alginate lyases Alginate oligosaccharides Dorea sp. Gut microbiota

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Point-of-care ultrasonography in geriatric medicine: usefulness for approaching infectious endocarditis diagnosis

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Journal of Geriatric Cardiology 2022年第12期19卷 1001-1002页

作者： Pablo Solla Patricia Cancelo Eva lópez Jesús de la hera César Morís José Gutiérrez Geriatrics Department Geriatrics Clinical Management AreaHospital Monte NarancoOviedoSpain health Research Institute of Asturias OviedoSpain Cardiology Department Cardiac AreaHospital Universitario Central de AsturiasOviedoSpain

Infective endocarditis has a high morbimortality rate,and delay in diagnosis and treatment is associated with a higher prevalence of *** clinical presentation is often atypical in older adults,and even when the classic symptoms are present,they may overlap with those of other conditions,making management more *** present the case of a nonagenarian in whom cardiac-centered point-of-care ultrasound facilitated real-time decision making and the diagnosis of mitral endocarditis.

关键词： diagnosis endocarditis mortality

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Post-hepatectomy liver failure:A timeline centered review

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hepatobiliary & Pancreatic Diseases International 2023年第6期22卷 554-569页

作者： Mohamed Bekheit lisa Grundy Ahmed ka Salih Petru Bucur Eric Vibert Mudassar Ghazanfar Department of Surgery NHS GrampianForesterhill Health CampusAshgrove RoadAB252ZN AberdeenUK Institute of Medical Sciences Medical SchoolForesterhill Health CampusAshgrove RoadAB252ZN AberdeenUK hépatica Integrated Center of HPB CareElite HospitalAgriculture RoadAlexandriaEgypt Department of Surgery University Hospital ToursVal de la Loire 37000France Centre hépatobiliaire Paul Brousse Hospital12 Paul Valliant Couturier94804 VillejuifFrance

Background: Post-hepatectomy liver failure(PhlF) is a leading cause of postoperative mortality after liver surgery. Due to its significant impact, it is imperative to understand the risk stratification and preventative strategies for PhlF. The main objective of this review is to highlight the role of these strategies in a timeline centered way around curative resection. Data sources: This review includes studies on both humans and animals, where they addressed PhlF. A literature search was conducted across the Cochrane library, Embase, MEDlINE/Pub Med, and Web of Knowledge electronic databases for English language studies published between July 1997 and June 2020. Studies presented in other languages were equally considered. The quality of included publications was assessed using Downs and Black’s checklist. The results were presented in qualitative summaries owing to the lack of studies qualifying for quantitative analysis. Results: This systematic review with 245 studies, provides insight into the current prediction, prevention, diagnosis, and management options for PhlF. This review highlighted that liver volume manipulation is the most frequently studied preventive measure against PhlF in clinical practice, with modest improvement in the treatment strategies over the past decade. Conclusions: Remnant liver volume manipulation is the most consistent preventive measure against PhlF.

关键词： Post-hepatectomy liver failure liver neoplasms Prediction Prevention Management

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Treatment of intractable orbital implant exposure with a large conjunctival defect by secondary insertion of the implant after preceding dermis fat graft

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International Journal of Ophthalmology(English edition) 2013年第2期6卷 193-197页

作者： hyun Kyung Kim Tae Yoon la Department of Ophthalmology St. Vincent's Hospital College of Medicine The Catholic University of Korea

AIM: To report a procedure and results of a two-stage operation to manage intractable extensive orbital implant exposure with a large conjunctival defect which was difficult to treat with dermis fat grafts due to repeated graft necrosis. METhODS: A retrospective chart review of four patients who had extensive orbital implant exposures with large conjunctival defects and had past histories of repeated autologous or preserved dermis graft failures was done. As a first-stage operation, the problematic pre-existing orbital implants were removed and autologous dermis fat grafts alone were performed on the defect area. Four months later, new orbital implants were secondarily inserted after confirmation of graft survival. The size of the conjunctival defects and state of the extraocular muscles were checked preoperatively. Success of the operations and complications were ***: The mean size of the conjuctival defects was 17.3mm×16.0mm, and the mean time from the initial diagnosis of orbital implant exposure to implant removal and autologous dermis fat graft was 20.8 months. After implant removal and autologous dermis fat graft, no graft necrosis was observed in any patients. Also, implant exposure or fornix shortening was not observed in any patients after new orbital implant insertion. CONClUSION: The secondary insertion of a new orbital implant after pre-existing implant removal and preceding dermis fat graft is thought to be an another selective management of intractable orbital implant exposure in which dermis fat grafts persistently fail.

关键词： conjunctival defect dermis fat graft orbital implant exposure

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An Enterococcus faecalis isolate harboring both aerobic and anaerobic uric acid metabolic pathways alleviates hyperuricemia in mice

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Food Science and human Wellness 2024年

作者： Depeng li Xinyue Yang A la Teng Zhu la Meihong Zhang Zhengtian lü Yuqing Feng Dan liu Yuming Guo Yongfei hu State Key laboratory of Animal Nutrition and Feeding， College of Animal Science and Technology， China Agricultural University

Probiotics have been demonstrated to be effective and safe for hyperuricemia (hUA) and gout treatment. hence， identifying microbes with high uric acid- or purine-metabolizing ability is highly important. Due to the high load of uric acid in chicken feces， we hypothesized that the chicken gut would be an excellent source for new uric acid or purine-metabolizing microbes. In this study， we examined chicken gut contents to isolate an Enterococcus faecalis strain (designated CMl390) that displayed high uric acid-degrading ability. In vitro experiments indicated that E. faecalis CMl390 could effectively metabolize uric acid under both aerobic and anaerobic conditions， and the richer the nutrients， the higher the rate of uric acid degradation. By integrating genomics， transcriptomics， and metabolomics analyses， we revealed two pathways involved in aerobic and anaerobic uric acid degradation， respectively， in this isolate. E. faecalis CMl390 relies on oxygen to metabolize uric acid into substances such as allantoin under aerobic conditions， while it also harbors a conserved uric acid-inducible gene cluster for anaerobic uric acid metabolism. An in vivo experiment showed that gavage with live E. faecalis CMl390 in a mouse model of hUA (established using a combination of uric acid and potassium oxonate) significantly reduced serum uric acid levels and alleviated hUA. Our findings contribute to the exploration of the pathways and mechanisms of uric acid metabolism in intestinal microorganisms and provide a candidate probiotic for the treatment of hUA and gout.

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Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane-cell wall nexus

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Journal of Integrative Plant Biology 2018年第8期60卷 649-669页

作者： Trevor h.Yeats Antony Bacic Kim l.Johnson School of Integrated Plant Sciences Section of Plant Biology Cornell University Robert W.holley Center for Agriculture and health USDA-ARS Cornell University Australian Research Council Centre of Excellence in Plant Cell Walls School of Bio Sciences University of Melbourne la Trobe Institute for Agriculture & Food Department of Animal Plant and Soil Sciences La Trobe University

Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphospha- tidylinositol （GPI） anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. Whereas the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPl-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPl-anchored proteome being further modified with plant-specific arabinogalactan （AG） O-glycans. The impor- tance of the GPl-anchored proteome to plant development is underscored by the fact that GPI biosynthetic null mutants exhibit embryo lethality. Mutations in genes encoding specific GPl-anchored proteins （GAPs） further supports their contribution to diverse biological processes, occurring at the interface of the plasma membrane and cell wall, including signaling, cell wall metabolism, cell wall polymer cross-linking, and plasmodesmatal transport. here, we review the literature concerning plant GPl-anchored proteins, in the context of their potential to act as molecular hubs that mediate interactions between the plasma membrane and the cell wall, and their potential to transduce the signal into the protoplast and, thereby, activate signal transduction pathways.

关键词： GPI Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane-cell wall nexus

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First Total Synthesis of Optically Active Oplopandiol Acetate, a Potent Antimycobacterial Polyyne Isolated from Oplopanax horridus

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Chinese Chemical letters 2000年第3期11卷 213-216页

作者： Xu, la Wu, Xh Zheng, GR Cai, JC Chinese Acad Sci Shanghai Inst Mat Med Shanghai 200031 Peoples R China

The first stereoselective total synthesis of oplopandiol acetate 1, a potent antimycobacterial polyyne isolated from Oplopanax horridus, is presented. And its absolute configuration is confirmed to be (11S,16S).

关键词： total synthesis oplopandiol acetate

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